{"id":"https://openalex.org/W4384929567","doi":"https://doi.org/10.3390/s23146544","title":"A Lateral Flow Assay for the Detection of Leptospira\u00a0lipL32 Gene Using CRISPR Technology","display_name":"A Lateral Flow Assay for the Detection of Leptospira\u00a0lipL32 Gene Using CRISPR Technology","publication_year":2023,"publication_date":"2023-07-20","ids":{"openalex":"https://openalex.org/W4384929567","doi":"https://doi.org/10.3390/s23146544","pmid":"https://pubmed.ncbi.nlm.nih.gov/37514838"},"language":"en","primary_location":{"id":"doi:10.3390/s23146544","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s23146544","pdf_url":"https://www.mdpi.com/1424-8220/23/14/6544/pdf?version=1689836070","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1424-8220/23/14/6544/pdf?version=1689836070","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054839222","display_name":"Satheesh Natarajan","orcid":"https://orcid.org/0000-0002-1045-7472"},"institutions":[{"id":"https://openalex.org/I24676775","display_name":"Indian Institute of Technology Madras","ror":"https://ror.org/03v0r5n49","country_code":"IN","type":"education","lineage":["https://openalex.org/I24676775"]},{"id":"https://openalex.org/I4210102197","display_name":"Healthcare Technology Innovation Centre","ror":"https://ror.org/017mw4x28","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210102197"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Satheesh Natarajan","raw_affiliation_strings":["Healthcare Technology Innovation Centre, Indian Institute of Technology Madras, Chennai 600113, India"],"raw_orcid":"https://orcid.org/0000-0002-1045-7472","affiliations":[{"raw_affiliation_string":"Healthcare Technology Innovation Centre, Indian Institute of Technology Madras, Chennai 600113, India","institution_ids":["https://openalex.org/I24676775","https://openalex.org/I4210102197"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020295407","display_name":"Jayaraj Joseph","orcid":"https://orcid.org/0000-0002-7279-9099"},"institutions":[{"id":"https://openalex.org/I24676775","display_name":"Indian Institute of Technology Madras","ror":"https://ror.org/03v0r5n49","country_code":"IN","type":"education","lineage":["https://openalex.org/I24676775"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Jayaraj Joseph","raw_affiliation_strings":["Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai 600036, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical Engineering, Indian Institute of Technology Madras, Chennai 600036, India","institution_ids":["https://openalex.org/I24676775"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077454819","display_name":"V. Balamurugan","orcid":"https://orcid.org/0000-0001-7821-7427"},"institutions":[{"id":"https://openalex.org/I179420787","display_name":"Indian Council of Agricultural Research","ror":"https://ror.org/04fw54a43","country_code":"IN","type":"government","lineage":["https://openalex.org/I179420787"]},{"id":"https://openalex.org/I4210160780","display_name":"National Institute Of Veterinary Epidemiology And Disease Informatics","ror":"https://ror.org/04s9fyw02","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I4210160780"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Balamurugan Vinayagamurthy","raw_affiliation_strings":["Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Bangalore 560064, India"],"raw_orcid":"https://orcid.org/0000-0001-7821-7427","affiliations":[{"raw_affiliation_string":"Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Bangalore 560064, India","institution_ids":["https://openalex.org/I179420787","https://openalex.org/I4210160780"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069467963","display_name":"Pedro Estrela","orcid":"https://orcid.org/0000-0001-6956-1146"},"institutions":[{"id":"https://openalex.org/I51601045","display_name":"University of Bath","ror":"https://ror.org/002h8g185","country_code":"GB","type":"education","lineage":["https://openalex.org/I51601045"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Pedro Estrela","raw_affiliation_strings":["Centre for Bioengineering & Biomedical Technologies, University of Bath, Bath BA2 7AY, UK","Department of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, UK"],"raw_orcid":"https://orcid.org/0000-0001-6956-1146","affiliations":[{"raw_affiliation_string":"Centre for Bioengineering & Biomedical Technologies, University of Bath, Bath BA2 7AY, UK","institution_ids":["https://openalex.org/I51601045"]},{"raw_affiliation_string":"Department of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, UK","institution_ids":["https://openalex.org/I51601045"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5054839222"],"corresponding_institution_ids":["https://openalex.org/I24676775","https://openalex.org/I4210102197"],"apc_list":{"value":2400,"currency":"CHF","value_usd":2673},"apc_paid":{"value":2400,"currency":"CHF","value_usd":2673},"fwci":1.6787,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.80769609,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"23","issue":"14","first_page":"6544","last_page":"6544"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/leptospirosis","display_name":"Leptospirosis","score":0.8935068845748901},{"id":"https://openalex.org/keywords/leptospira","display_name":"Leptospira","score":0.7344125509262085},{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.6731075048446655},{"id":"https://openalex.org/keywords/dengue-fever","display_name":"Dengue fever","score":0.6162623763084412},{"id":"https://openalex.org/keywords/polymerase-chain-reaction","display_name":"Polymerase chain reaction","score":0.5477955341339111},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.38622480630874634},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.38340502977371216},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.3807823359966278},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.34218674898147583},{"id":"https://openalex.org/keywords/virology","display_name":"Virology","score":0.31263023614883423},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.15915295481681824}],"concepts":[{"id":"https://openalex.org/C2778033228","wikidata":"https://www.wikidata.org/wiki/Q155098","display_name":"Leptospirosis","level":2,"score":0.8935068845748901},{"id":"https://openalex.org/C2780542935","wikidata":"https://www.wikidata.org/wiki/Q133841","display_name":"Leptospira","level":3,"score":0.7344125509262085},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.6731075048446655},{"id":"https://openalex.org/C533803919","wikidata":"https://www.wikidata.org/wiki/Q30953","display_name":"Dengue fever","level":2,"score":0.6162623763084412},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5477955341339111},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38622480630874634},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.38340502977371216},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3807823359966278},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34218674898147583},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.31263023614883423},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15915295481681824}],"mesh":[{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001425","descriptor_name":"Bacterial Outer Membrane Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D008074","descriptor_name":"Lipoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008074","descriptor_name":"Lipoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008074","descriptor_name":"Lipoproteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.3390/s23146544","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s23146544","pdf_url":"https://www.mdpi.com/1424-8220/23/14/6544/pdf?version=1689836070","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},{"id":"pmid:37514838","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37514838","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors (Basel, Switzerland)","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:10385553","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10385553","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10385553/pdf/sensors-23-06544.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors (Basel)","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:a720c7c4c0274b158faea6049bb73eb4","is_oa":true,"landing_page_url":"https://doaj.org/article/a720c7c4c0274b158faea6049bb73eb4","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors, Vol 23, Iss 14, p 6544 (2023)","raw_type":"article"},{"id":"pmh:oai:mdpi.com:/1424-8220/23/14/6544/","is_oa":true,"landing_page_url":"https://dx.doi.org/10.3390/s23146544","pdf_url":null,"source":{"id":"https://openalex.org/S4306400947","display_name":"MDPI (MDPI AG)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I4210097602","host_organization_name":"Multidisciplinary Digital Publishing Institute (Switzerland)","host_organization_lineage":["https://openalex.org/I4210097602"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors; Volume 23; Issue 14; Pages: 6544","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3390/s23146544","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s23146544","pdf_url":"https://www.mdpi.com/1424-8220/23/14/6544/pdf?version=1689836070","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.6399999856948853}],"awards":[],"funders":[{"id":"https://openalex.org/F4320320769","display_name":"Indian Council of Agricultural Research","ror":"https://ror.org/04fw54a43"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4384929567.pdf"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W100350201","https://openalex.org/W1845579074","https://openalex.org/W1863141132","https://openalex.org/W1874879909","https://openalex.org/W1948094720","https://openalex.org/W2001792051","https://openalex.org/W2024457326","https://openalex.org/W2050753962","https://openalex.org/W2581123597","https://openalex.org/W2754221693","https://openalex.org/W2769850348","https://openalex.org/W2799873087","https://openalex.org/W2884548398","https://openalex.org/W2908088254","https://openalex.org/W3082124825","https://openalex.org/W3132332958","https://openalex.org/W3169981325","https://openalex.org/W3178418858","https://openalex.org/W4200160212","https://openalex.org/W4210680521","https://openalex.org/W4283781744","https://openalex.org/W6656599262"],"related_works":["https://openalex.org/W4283816792","https://openalex.org/W1968903599","https://openalex.org/W2895360593","https://openalex.org/W2891142468","https://openalex.org/W2020260041","https://openalex.org/W1967024214","https://openalex.org/W2205587226","https://openalex.org/W2398575331","https://openalex.org/W3192506268","https://openalex.org/W2736551251"],"abstract_inverted_index":{"The":[0,101,115],"clinical":[1],"manifestation":[2],"of":[3,70,88,112],"leptospirosis":[4,80,124],"is":[5,17,50,61,117],"often":[6],"misdiagnosed":[7],"as":[8,13,53],"other":[9],"febrile":[10],"illnesses":[11],"such":[12],"dengue.":[14],"Therefore,":[15],"there":[16],"an":[18],"urgent":[19],"need":[20],"for":[21,41],"a":[22,89,130],"precise":[23],"diagnostic":[24,56],"tool":[25],"at":[26,37],"the":[27,32,38,54,68,86,98,108],"field":[28],"level":[29,40],"to":[30,96],"detect":[31,97],"pathogenic":[33],"Leptospira":[34],"lipL32":[35,99],"gene":[36],"molecular":[39],"prompt":[42],"therapeutic":[43],"decisions.":[44],"Quantitative":[45],"polymerase":[46],"chain":[47],"reaction":[48],"(qPCR)":[49],"widely":[51],"used":[52],"primary":[55],"tool,":[57],"but":[58],"its":[59],"applicability":[60],"limited":[62],"by":[63],"high":[64],"equipment":[65],"cost":[66],"and":[67,120],"lack":[69],"availability":[71],"in":[72,76],"every":[73],"hospital,":[74],"especially":[75],"rural":[77],"areas":[78],"where":[79],"mainly":[81],"occurs.":[82],"Here,":[83],"we":[84],"report":[85],"development":[87],"CRISPR":[90],"dFnCas9-based":[91],"quantitative":[92],"lateral":[93,132],"flow":[94,133],"immunoassay":[95],"gene.":[100],"developed":[102],"assay":[103],"showed":[104],"superior":[105],"performance":[106],"regarding":[107],"lowest":[109],"detectable":[110],"limit":[111],"1":[113],"fg/mL.":[114],"test":[116],"highly":[118],"sensitive":[119],"selective,":[121],"showing":[122],"that":[123],"diagnosis":[125],"can":[126],"be":[127],"achieved":[128],"with":[129],"low-cost":[131],"device.":[134]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1}],"updated_date":"2026-07-22T07:51:19.307946","created_date":"2025-10-10T00:00:00"}