{"id":"https://openalex.org/W2134459523","doi":"https://doi.org/10.3390/s130404553","title":"Internal Calibration F\u00f6rster Resonance Energy Transfer Assay: A Real-Time Approach for Determining Protease Kinetics","display_name":"Internal Calibration F\u00f6rster Resonance Energy Transfer Assay: A Real-Time Approach for Determining Protease Kinetics","publication_year":2013,"publication_date":"2013-04-08","ids":{"openalex":"https://openalex.org/W2134459523","doi":"https://doi.org/10.3390/s130404553","mag":"2134459523"},"language":"en","primary_location":{"id":"doi:10.3390/s130404553","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s130404553","pdf_url":"https://www.mdpi.com/1424-8220/13/4/4553/pdf?version=1403326319","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1424-8220/13/4/4553/pdf?version=1403326319","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101786019","display_name":"Ling Jiang","orcid":"https://orcid.org/0000-0003-3331-6207"},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]},{"id":"https://openalex.org/I169572211","display_name":"Northeast Agricultural University","ror":"https://ror.org/0515nd386","country_code":"CN","type":"education","lineage":["https://openalex.org/I169572211"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Ling Jiang","raw_affiliation_strings":["Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, Heilongjiang, China"],"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, Heilongjiang, China","institution_ids":["https://openalex.org/I169572211"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100351064","display_name":"Yan Liu","orcid":"https://orcid.org/0000-0002-2566-6867"},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yan Liu","raw_affiliation_strings":["Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA"],"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046721150","display_name":"Yang Song","orcid":"https://orcid.org/0000-0001-7716-9216"},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yang Song","raw_affiliation_strings":["Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA"],"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020616702","display_name":"Amanda Saavedra","orcid":null},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amanda Saavedra","raw_affiliation_strings":["Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA"],"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001697817","display_name":"Songqin Pan","orcid":null},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Songqin Pan","raw_affiliation_strings":["Institute for Integrative Genome Biology, University of California at Riverside,  900 University Avenue, Riverside, CA 92521, USA","Keck Proteomics Laboratory, Institute for Integrative Genome Biology,  University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","Keck Proteomics Laboratory, Institute for Integrative Genome Biology, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA"],"affiliations":[{"raw_affiliation_string":"Institute for Integrative Genome Biology, University of California at Riverside,  900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Keck Proteomics Laboratory, Institute for Integrative Genome Biology,  University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Keck Proteomics Laboratory, Institute for Integrative Genome Biology, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060857562","display_name":"Wensheng Xiang","orcid":"https://orcid.org/0000-0002-1826-5985"},"institutions":[{"id":"https://openalex.org/I169572211","display_name":"Northeast Agricultural University","ror":"https://ror.org/0515nd386","country_code":"CN","type":"education","lineage":["https://openalex.org/I169572211"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wensheng Xiang","raw_affiliation_strings":["School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, Heilongjiang, China"],"affiliations":[{"raw_affiliation_string":"School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, Heilongjiang, China","institution_ids":["https://openalex.org/I169572211"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061097665","display_name":"Jiayu Liao","orcid":"https://orcid.org/0000-0002-2624-5781"},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jiayu Liao","raw_affiliation_strings":["Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","Institute for Integrative Genome Biology, University of California at Riverside,  900 University Avenue, Riverside, CA 92521, USA","Institute for Integrative Genome Biology, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA"],"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, Center for Bioengineering Research, Bourns College of Engineering, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Institute for Integrative Genome Biology, University of California at Riverside,  900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Institute for Integrative Genome Biology, University of California at Riverside, 900 University Avenue, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5061097665"],"corresponding_institution_ids":["https://openalex.org/I103635307"],"apc_list":{"value":2400,"currency":"CHF","value_usd":2598},"apc_paid":{"value":2400,"currency":"CHF","value_usd":2598},"fwci":0.5989,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.69679365,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"13","issue":"4","first_page":"4553","last_page":"4570"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":0.9972000122070312,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11150","display_name":"Endoplasmic Reticulum Stress and Disease","score":0.994700014591217,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/f\u00f6rster-resonance-energy-transfer","display_name":"F\u00f6rster resonance energy transfer","score":0.960900068283081},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"Enzyme kinetics","score":0.7355262041091919},{"id":"https://openalex.org/keywords/sumo-protein","display_name":"SUMO protein","score":0.728843092918396},{"id":"https://openalex.org/keywords/proteases","display_name":"Proteases","score":0.7243328094482422},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.5975120067596436},{"id":"https://openalex.org/keywords/kinetics","display_name":"Kinetics","score":0.5143598318099976},{"id":"https://openalex.org/keywords/protease","display_name":"Protease","score":0.46633028984069824},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.4649805724620819},{"id":"https://openalex.org/keywords/acceptor","display_name":"Acceptor","score":0.45645663142204285},{"id":"https://openalex.org/keywords/substrate","display_name":"Substrate (aquarium)","score":0.44074875116348267},{"id":"https://openalex.org/keywords/biophysics","display_name":"Biophysics","score":0.4385482668876648},{"id":"https://openalex.org/keywords/enzyme","display_name":"Enzyme","score":0.2908502519130707},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.219375878572464},{"id":"https://openalex.org/keywords/active-site","display_name":"Active site","score":0.16229262948036194},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.14706522226333618},{"id":"https://openalex.org/keywords/ubiquitin","display_name":"Ubiquitin","score":0.10274386405944824}],"concepts":[{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.960900068283081},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.7355262041091919},{"id":"https://openalex.org/C171350616","wikidata":"https://www.wikidata.org/wiki/Q61506","display_name":"SUMO protein","level":4,"score":0.728843092918396},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.7243328094482422},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5975120067596436},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.5143598318099976},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.46633028984069824},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4649805724620819},{"id":"https://openalex.org/C2779892579","wikidata":"https://www.wikidata.org/wiki/Q912138","display_name":"Acceptor","level":2,"score":0.45645663142204285},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.44074875116348267},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4385482668876648},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2908502519130707},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.219375878572464},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.16229262948036194},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14706522226333618},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.10274386405944824},{"id":"https://openalex.org/C26873012","wikidata":"https://www.wikidata.org/wiki/Q214781","display_name":"Condensed matter physics","level":1,"score":0.0},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/s130404553","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s130404553","pdf_url":"https://www.mdpi.com/1424-8220/13/4/4553/pdf?version=1403326319","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.414.5767","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.414.5767","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.mdpi.com/1424-8220/13/4/4553/pdf?y=1","raw_type":"text"},{"id":"pmh:oai:doaj.org/article:8fa66f919db64724a55cb2ad7481a84b","is_oa":true,"landing_page_url":"https://doaj.org/article/8fa66f919db64724a55cb2ad7481a84b","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors, Vol 13, Iss 4, Pp 4553-4570 (2013)","raw_type":"article"},{"id":"pmh:oai:europepmc.org:2681379","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3673099","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3390/s130404553","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s130404553","pdf_url":"https://www.mdpi.com/1424-8220/13/4/4553/pdf?version=1403326319","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8999999761581421,"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2134459523.pdf","grobid_xml":"https://content.openalex.org/works/W2134459523.grobid-xml"},"referenced_works_count":29,"referenced_works":["https://openalex.org/W1520109974","https://openalex.org/W1969301826","https://openalex.org/W1972653948","https://openalex.org/W1973480232","https://openalex.org/W1993180829","https://openalex.org/W1996880521","https://openalex.org/W2012618272","https://openalex.org/W2013108773","https://openalex.org/W2019437977","https://openalex.org/W2021670268","https://openalex.org/W2031090055","https://openalex.org/W2040494716","https://openalex.org/W2051166632","https://openalex.org/W2052388749","https://openalex.org/W2056801882","https://openalex.org/W2068924803","https://openalex.org/W2070270322","https://openalex.org/W2077628671","https://openalex.org/W2082056621","https://openalex.org/W2112441454","https://openalex.org/W2113191661","https://openalex.org/W2124411936","https://openalex.org/W2125074509","https://openalex.org/W2134270207","https://openalex.org/W2136128861","https://openalex.org/W2139364069","https://openalex.org/W2153789013","https://openalex.org/W2170632398","https://openalex.org/W2172006299"],"related_works":["https://openalex.org/W2053523004","https://openalex.org/W4286007827","https://openalex.org/W2048074445","https://openalex.org/W2142891272","https://openalex.org/W1599075286","https://openalex.org/W2112976975","https://openalex.org/W1988949448","https://openalex.org/W2797913390","https://openalex.org/W1987066967","https://openalex.org/W2079357620"],"abstract_inverted_index":{"F\u00f6rster":[0],"resonance":[1],"energy":[2],"transfer":[3],"(FRET)":[4],"technology":[5],"has":[6],"been":[7],"widely":[8],"used":[9],"in":[10,22,46,154,236],"biological":[11,73],"and":[12,43,117,140,147,157,195,204,239],"biomedical":[13],"research.":[14],"This":[15,64],"powerful":[16],"tool":[17],"can":[18,230],"elucidate":[19],"protein":[20,39,67],"interactions":[21],"either":[23],"a":[24,32,97,237],"dynamic":[25],"or":[26,85],"steady":[27],"state.":[28],"We":[29],"recently":[30],"developed":[31,184],"series":[33],"of":[34,50,107,119,134,144,208,220,225],"FRET-based":[35,100,226],"technologies":[36],"to":[37,58,81,88,185,233],"determine":[38,186],"interaction":[40],"dissociation":[41],"constant":[42],"for":[44,71,103,212],"use":[45],"high-throughput":[47],"screening":[48],"assays":[49],"SUMOylation.":[51],"SUMO":[52,90],"(small":[53],"ubiquitin-like":[54],"modifier)":[55],"is":[56,69,111],"conjugated":[57],"substrates":[59],"through":[60],"an":[61],"enzymatic":[62],"cascade.":[63],"important":[65],"posttranslational":[66],"modification":[68],"critical":[70],"multiple":[72],"processes.":[74],"Sentrin/SUMO-specific":[75],"proteases":[76,235],"(SENPs)":[77],"act":[78],"as":[79,86],"endopeptidases":[80],"process":[82,177],"the":[83,105,114,124,137,141,166,174,192,217],"pre-SUMO":[84],"isopeptidases":[87],"deconjugate":[89],"from":[91,178,191],"its":[92],"substrate.":[93],"Here,":[94],"we":[95],"describe":[96],"novel":[98],"quantitative":[99,115,223],"protease":[101,227],"assay":[102],"determining":[104],"kinetics":[106],"SENP1.":[108],"Our":[109],"strategy":[110],"based":[112],"on":[113],"analysis":[116],"differentiation":[118],"fluorescent":[120,130,142,168],"emission":[121,127,131],"signals":[122,132],"at":[123],"FRET":[125,138,193],"acceptor":[126,148,158],"wavelengths.":[128],"Those":[129],"consist":[133],"three":[135,167],"components:":[136],"signal":[139,194],"emissions":[143,160,169],"donor":[145,156],"(CyPet)":[146],"(YPet).":[149],"Unlike":[150],"our":[151],"previous":[152],"method":[153],"which":[155],"direct":[159,197],"were":[161,170,183,214],"excluded":[162],"by":[163],"standard":[164],"curves,":[165],"determined":[171],"quantitatively":[172],"during":[173],"SENP":[175],"digestion":[176],"onesample.":[179],"New":[180],"mathematical":[181],"algorithms":[182],"digested":[187],"substrate":[188],"concentrations":[189],"directly":[190],"donor/acceptor":[196],"emissions.":[198],"The":[199],"kinetic":[200,228],"parameters,":[201],"kcat,":[202],"KM,":[203],"catalytic":[205,210],"efficiency":[206],"(kcat/KM)":[207],"SENP1":[209],"domain":[211],"pre-SUMO1/2/3":[213],"derived.":[215],"Importantly,":[216],"general":[218],"principles":[219],"this":[221],"new":[222],"methodology":[224],"determinations":[229],"be":[231],"applied":[232],"other":[234],"robust":[238],"systems":[240],"biology":[241],"approach.":[242]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":4}],"updated_date":"2026-04-04T16:13:02.066488","created_date":"2025-10-10T00:00:00"}