{"id":"https://openalex.org/W1974671435","doi":"https://doi.org/10.3390/s100100697","title":"Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System","display_name":"Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System","publication_year":2010,"publication_date":"2010-01-19","ids":{"openalex":"https://openalex.org/W1974671435","doi":"https://doi.org/10.3390/s100100697","mag":"1974671435","pmid":"https://pubmed.ncbi.nlm.nih.gov/22315563"},"language":"en","primary_location":{"id":"doi:10.3390/s100100697","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s100100697","pdf_url":"https://www.mdpi.com/1424-8220/10/1/697/pdf?version=1403312079","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/1424-8220/10/1/697/pdf?version=1403312079","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033173613","display_name":"Dasheng Lee","orcid":"https://orcid.org/0000-0002-1182-5070"},"institutions":[{"id":"https://openalex.org/I118292597","display_name":"National Taipei University of Technology","ror":"https://ror.org/00cn92c09","country_code":"TW","type":"education","lineage":["https://openalex.org/I118292597"]}],"countries":["TW"],"is_corresponding":true,"raw_author_name":"Da-Sheng Lee","raw_affiliation_strings":["Department of Energy and Refrigerating Air Conditioning Engineering, National Taipei University of Technology, Taipei, Taiwan"],"affiliations":[{"raw_affiliation_string":"Department of Energy and Refrigerating Air Conditioning Engineering, National Taipei University of Technology, Taipei, Taiwan","institution_ids":["https://openalex.org/I118292597"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5033173613"],"corresponding_institution_ids":["https://openalex.org/I118292597"],"apc_list":{"value":2400,"currency":"CHF","value_usd":2598},"apc_paid":{"value":2400,"currency":"CHF","value_usd":2598},"fwci":0.2703,"has_fulltext":true,"cited_by_count":14,"citation_normalized_percentile":{"value":0.57768634,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"10","issue":"1","first_page":"697","last_page":"718"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9918000102043152,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9908000230789185,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/robustness","display_name":"Robustness (evolution)","score":0.6525478363037109},{"id":"https://openalex.org/keywords/chip","display_name":"Chip","score":0.6174896359443665},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5153283476829529},{"id":"https://openalex.org/keywords/system-on-a-chip","display_name":"System on a chip","score":0.5124319195747375},{"id":"https://openalex.org/keywords/reliability-engineering","display_name":"Reliability engineering","score":0.4956170320510864},{"id":"https://openalex.org/keywords/lift","display_name":"Lift (data mining)","score":0.48793432116508484},{"id":"https://openalex.org/keywords/lab-on-a-chip","display_name":"Lab-on-a-chip","score":0.4393681585788727},{"id":"https://openalex.org/keywords/reliability","display_name":"Reliability (semiconductor)","score":0.42799103260040283},{"id":"https://openalex.org/keywords/embedded-system","display_name":"Embedded system","score":0.38618502020835876},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.33382469415664673},{"id":"https://openalex.org/keywords/data-mining","display_name":"Data mining","score":0.14304515719413757},{"id":"https://openalex.org/keywords/microfluidics","display_name":"Microfluidics","score":0.12049740552902222}],"concepts":[{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.6525478363037109},{"id":"https://openalex.org/C165005293","wikidata":"https://www.wikidata.org/wiki/Q1074500","display_name":"Chip","level":2,"score":0.6174896359443665},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5153283476829529},{"id":"https://openalex.org/C118021083","wikidata":"https://www.wikidata.org/wiki/Q610398","display_name":"System on a chip","level":2,"score":0.5124319195747375},{"id":"https://openalex.org/C200601418","wikidata":"https://www.wikidata.org/wiki/Q2193887","display_name":"Reliability engineering","level":1,"score":0.4956170320510864},{"id":"https://openalex.org/C139002025","wikidata":"https://www.wikidata.org/wiki/Q3001212","display_name":"Lift (data mining)","level":2,"score":0.48793432116508484},{"id":"https://openalex.org/C138942068","wikidata":"https://www.wikidata.org/wiki/Q633261","display_name":"Lab-on-a-chip","level":3,"score":0.4393681585788727},{"id":"https://openalex.org/C43214815","wikidata":"https://www.wikidata.org/wiki/Q7310987","display_name":"Reliability (semiconductor)","level":3,"score":0.42799103260040283},{"id":"https://openalex.org/C149635348","wikidata":"https://www.wikidata.org/wiki/Q193040","display_name":"Embedded system","level":1,"score":0.38618502020835876},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.33382469415664673},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.14304515719413757},{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.12049740552902222},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C163258240","wikidata":"https://www.wikidata.org/wiki/Q25342","display_name":"Power (physics)","level":2,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003199","descriptor_name":"Computer Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003199","descriptor_name":"Computer Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003199","descriptor_name":"Computer Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004867","descriptor_name":"Equipment Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004867","descriptor_name":"Equipment Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004867","descriptor_name":"Equipment Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017076","descriptor_name":"Computer-Aided Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017076","descriptor_name":"Computer-Aided Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017076","descriptor_name":"Computer-Aided Design","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019544","descriptor_name":"Equipment Failure Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019544","descriptor_name":"Equipment Failure Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019544","descriptor_name":"Equipment Failure Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D020411","descriptor_name":"Oligonucleotide Array Sequence Analysis","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.3390/s100100697","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s100100697","pdf_url":"https://www.mdpi.com/1424-8220/10/1/697/pdf?version=1403312079","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},{"id":"pmid:22315563","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22315563","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors (Basel, Switzerland)","raw_type":null},{"id":"pmh:oai:doaj.org/article:25d30a054c104d19b8f7eeb96cb50228","is_oa":true,"landing_page_url":"https://doaj.org/article/25d30a054c104d19b8f7eeb96cb50228","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors, Vol 10, Iss 1, Pp 697-718 (2010)","raw_type":"article"},{"id":"pmh:oai:europepmc.org:2281352","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3270864","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:mdpi.com:/1424-8220/10/1/697/","is_oa":true,"landing_page_url":"https://dx.doi.org/10.3390/s100100697","pdf_url":null,"source":{"id":"https://openalex.org/S4306400947","display_name":"MDPI (MDPI AG)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I4210097602","host_organization_name":"Multidisciplinary Digital Publishing Institute (Switzerland)","host_organization_lineage":["https://openalex.org/I4210097602"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sensors; Volume 10; Issue 1; Pages: 697-718","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3390/s100100697","is_oa":true,"landing_page_url":"https://doi.org/10.3390/s100100697","pdf_url":"https://www.mdpi.com/1424-8220/10/1/697/pdf?version=1403312079","source":{"id":"https://openalex.org/S101949793","display_name":"Sensors","issn_l":"1424-8220","issn":["1424-8220"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sensors","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4300000071525574,"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320324663","display_name":"National Cheng Kung University","ror":"https://ror.org/01b8kcc49"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1974671435.pdf","grobid_xml":"https://content.openalex.org/works/W1974671435.grobid-xml"},"referenced_works_count":48,"referenced_works":["https://openalex.org/W1510554288","https://openalex.org/W1534486080","https://openalex.org/W1569947224","https://openalex.org/W1577577364","https://openalex.org/W1594653092","https://openalex.org/W1608192907","https://openalex.org/W1840387029","https://openalex.org/W1844220054","https://openalex.org/W1964596877","https://openalex.org/W1977771778","https://openalex.org/W1993080359","https://openalex.org/W1993231925","https://openalex.org/W2005400351","https://openalex.org/W2007575935","https://openalex.org/W2009491794","https://openalex.org/W2013835328","https://openalex.org/W2014780569","https://openalex.org/W2015783852","https://openalex.org/W2020918696","https://openalex.org/W2027598903","https://openalex.org/W2037045869","https://openalex.org/W2042578974","https://openalex.org/W2046052524","https://openalex.org/W2050395229","https://openalex.org/W2058665460","https://openalex.org/W2060197659","https://openalex.org/W2064290205","https://openalex.org/W2069775954","https://openalex.org/W2071352882","https://openalex.org/W2073857965","https://openalex.org/W2074660033","https://openalex.org/W2080347240","https://openalex.org/W2083675479","https://openalex.org/W2084354317","https://openalex.org/W2093451884","https://openalex.org/W2138321075","https://openalex.org/W2140858267","https://openalex.org/W2146069457","https://openalex.org/W2151053476","https://openalex.org/W2153489623","https://openalex.org/W2153534897","https://openalex.org/W2162321815","https://openalex.org/W2164095252","https://openalex.org/W2170994550","https://openalex.org/W4251261706","https://openalex.org/W4254533614","https://openalex.org/W6661126699","https://openalex.org/W6720614258"],"related_works":["https://openalex.org/W4389397071","https://openalex.org/W2770593030","https://openalex.org/W4281727072","https://openalex.org/W2560201613","https://openalex.org/W2136854845","https://openalex.org/W2375007538","https://openalex.org/W2115579119","https://openalex.org/W2017236304","https://openalex.org/W2387172946","https://openalex.org/W2569660137"],"abstract_inverted_index":{"Chip-based":[0],"DNA":[1,70,119],"quantification":[2,71],"systems":[3],"are":[4],"widespread,":[5],"and":[6,72],"used":[7],"in":[8,118],"many":[9],"point-of-care":[10],"applications.":[11],"However,":[12],"instruments":[13],"for":[14,31,77,111,140],"such":[15],"applications":[16],"may":[17],"not":[18,122],"be":[19],"maintained":[20],"or":[21],"calibrated":[22],"regularly.":[23],"Since":[24],"machine":[25,47],"reliability":[26,90],"is":[27],"a":[28,37,60,74,78,142],"key":[29],"issue":[30],"normal":[32],"operation,":[33],"this":[34,101],"study":[35],"presents":[36],"system":[38,105],"model":[39,58],"of":[40,69,91,127,136],"the":[41,50,67,89,92,97,125,128,134],"real-time":[42,79,103],"Polymerase":[43],"Chain":[44],"Reaction":[45],"(PCR)":[46],"to":[48,65,87,96,108],"analyze":[49],"instrument":[51],"design":[52,76],"through":[53],"numerical":[54],"experiments.":[55],"Based":[56],"on":[57],"analysis,":[59],"systematic":[61,138],"approach":[62],"was":[63,85,106],"developed":[64],"lower":[66],"variation":[68],"achieve":[73],"robust":[75,143],"PCR-on-a-chip":[80,104],"system.":[81],"Accelerated":[82],"lift":[83],"testing":[84],"adopted":[86],"evaluate":[88],"chip":[93],"prototype.":[94],"According":[95],"life":[98],"test":[99],"plan,":[100],"proposed":[102],"simulated":[107],"work":[109],"continuously":[110],"over":[112],"three":[113],"years":[114],"with":[115],"similar":[116],"reproducibility":[117],"quantification.":[120],"This":[121],"only":[123],"shows":[124],"robustness":[126],"lab-on-a-chip":[129],"system,":[130],"but":[131],"also":[132],"verifies":[133],"effectiveness":[135],"our":[137],"method":[139],"achieving":[141],"design.":[144]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-03-20T23:20:44.827607","created_date":"2025-10-10T00:00:00"}