{"id":"https://openalex.org/W2925203176","doi":"https://doi.org/10.3390/jimaging5040042","title":"Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly Zizeeria maha: Establishing the Lycaenid System for Multiscale Bioimaging","display_name":"Real-Time In Vivo Imaging of the Developing Pupal Wing Tissues in the Pale Grass Blue Butterfly Zizeeria maha: Establishing the Lycaenid System for Multiscale Bioimaging","publication_year":2019,"publication_date":"2019-03-28","ids":{"openalex":"https://openalex.org/W2925203176","doi":"https://doi.org/10.3390/jimaging5040042","mag":"2925203176","pmid":"https://pubmed.ncbi.nlm.nih.gov/34460480"},"language":"en","primary_location":{"id":"doi:10.3390/jimaging5040042","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jimaging5040042","pdf_url":"https://www.mdpi.com/2313-433X/5/4/42/pdf?version=1553779040","source":{"id":"https://openalex.org/S2736465063","display_name":"Journal of Imaging","issn_l":"2313-433X","issn":["2313-433X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Imaging","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2313-433X/5/4/42/pdf?version=1553779040","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113564359","display_name":"Kanako Hirata","orcid":null},"institutions":[{"id":"https://openalex.org/I32915989","display_name":"University of the Ryukyus","ror":"https://ror.org/02z1n9q24","country_code":"JP","type":"education","lineage":["https://openalex.org/I32915989"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kanako Hirata","raw_affiliation_strings":["The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan","institution_ids":["https://openalex.org/I32915989"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5041964842","display_name":"Joji M. Otaki","orcid":"https://orcid.org/0000-0003-3425-7085"},"institutions":[{"id":"https://openalex.org/I32915989","display_name":"University of the Ryukyus","ror":"https://ror.org/02z1n9q24","country_code":"JP","type":"education","lineage":["https://openalex.org/I32915989"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Joji M. Otaki","raw_affiliation_strings":["The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan"],"raw_orcid":"https://orcid.org/0000-0003-3425-7085","affiliations":[{"raw_affiliation_string":"The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan","institution_ids":["https://openalex.org/I32915989"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5041964842"],"corresponding_institution_ids":["https://openalex.org/I32915989"],"apc_list":{"value":1600,"currency":"CHF","value_usd":1732},"apc_paid":{"value":1600,"currency":"CHF","value_usd":1732},"fwci":0.8996,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.77360718,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"5","issue":"4","first_page":"42","last_page":"42"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10702","display_name":"Insect and Arachnid Ecology and Behavior","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10702","display_name":"Insect and Arachnid Ecology and Behavior","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10423","display_name":"Neurobiology and Insect Physiology Research","score":0.9986000061035156,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10174","display_name":"Animal Behavior and Reproduction","score":0.9977999925613403,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/wing","display_name":"Wing","score":0.6411672234535217},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.6348782777786255},{"id":"https://openalex.org/keywords/appendage","display_name":"Appendage","score":0.5876820087432861},{"id":"https://openalex.org/keywords/butterfly","display_name":"Butterfly","score":0.5020434856414795},{"id":"https://openalex.org/keywords/dorsum","display_name":"Dorsum","score":0.4937148988246918},{"id":"https://openalex.org/keywords/in-vivo","display_name":"In vivo","score":0.4255194365978241},{"id":"https://openalex.org/keywords/confocal-microscopy","display_name":"Confocal microscopy","score":0.4112969934940338},{"id":"https://openalex.org/keywords/anatomy","display_name":"Anatomy","score":0.35052406787872314},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.347267746925354},{"id":"https://openalex.org/keywords/ecology","display_name":"Ecology","score":0.12210538983345032}],"concepts":[{"id":"https://openalex.org/C97257150","wikidata":"https://www.wikidata.org/wiki/Q161358","display_name":"Wing","level":2,"score":0.6411672234535217},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6348782777786255},{"id":"https://openalex.org/C41451933","wikidata":"https://www.wikidata.org/wiki/Q13415392","display_name":"Appendage","level":2,"score":0.5876820087432861},{"id":"https://openalex.org/C2778605236","wikidata":"https://www.wikidata.org/wiki/Q1018085","display_name":"Butterfly","level":2,"score":0.5020434856414795},{"id":"https://openalex.org/C140530291","wikidata":"https://www.wikidata.org/wiki/Q1225063","display_name":"Dorsum","level":2,"score":0.4937148988246918},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4255194365978241},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.4112969934940338},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.35052406787872314},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.347267746925354},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.12210538983345032},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C146978453","wikidata":"https://www.wikidata.org/wiki/Q3798668","display_name":"Aerospace engineering","level":1,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.3390/jimaging5040042","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jimaging5040042","pdf_url":"https://www.mdpi.com/2313-433X/5/4/42/pdf?version=1553779040","source":{"id":"https://openalex.org/S2736465063","display_name":"Journal of Imaging","issn_l":"2313-433X","issn":["2313-433X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Imaging","raw_type":"journal-article"},{"id":"pmid:34460480","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/34460480","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of imaging","raw_type":null},{"id":"pmh:oai:doaj.org/article:bd01a61b47224dfd92ac10d40c5484ff","is_oa":true,"landing_page_url":"https://doaj.org/article/bd01a61b47224dfd92ac10d40c5484ff","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Imaging, Vol 5, Iss 4, p 42 (2019)","raw_type":"article"},{"id":"pmh:oai:mdpi.com:/2313-433X/5/4/42/","is_oa":true,"landing_page_url":"http://doi.org/10.3390/jimaging5040042","pdf_url":null,"source":{"id":"https://openalex.org/S4306400947","display_name":"MDPI (MDPI AG)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I4210097602","host_organization_name":"Multidisciplinary Digital Publishing Institute (Switzerland)","host_organization_lineage":["https://openalex.org/I4210097602"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Imaging","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:8320941","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8320941","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"J Imaging","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3390/jimaging5040042","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jimaging5040042","pdf_url":"https://www.mdpi.com/2313-433X/5/4/42/pdf?version=1553779040","source":{"id":"https://openalex.org/S2736465063","display_name":"Journal of Imaging","issn_l":"2313-433X","issn":["2313-433X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Imaging","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G6179220653","display_name":null,"funder_award_id":"JP16K07425","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":53,"referenced_works":["https://openalex.org/W1531106880","https://openalex.org/W1574977580","https://openalex.org/W1628414185","https://openalex.org/W1857260962","https://openalex.org/W1972838730","https://openalex.org/W1978780122","https://openalex.org/W1979977264","https://openalex.org/W1983742971","https://openalex.org/W1992166765","https://openalex.org/W2003247910","https://openalex.org/W2003922372","https://openalex.org/W2004104843","https://openalex.org/W2022155399","https://openalex.org/W2032579724","https://openalex.org/W2044089720","https://openalex.org/W2048850398","https://openalex.org/W2056822865","https://openalex.org/W2058019635","https://openalex.org/W2061319561","https://openalex.org/W2067222432","https://openalex.org/W2070669592","https://openalex.org/W2085987045","https://openalex.org/W2090410670","https://openalex.org/W2099540110","https://openalex.org/W2103014089","https://openalex.org/W2106515876","https://openalex.org/W2111008606","https://openalex.org/W2112298681","https://openalex.org/W2113548128","https://openalex.org/W2115823588","https://openalex.org/W2127103762","https://openalex.org/W2132253765","https://openalex.org/W2142916161","https://openalex.org/W2144518227","https://openalex.org/W2154456283","https://openalex.org/W2229362204","https://openalex.org/W2257866972","https://openalex.org/W2289318481","https://openalex.org/W2294886308","https://openalex.org/W2460080738","https://openalex.org/W2574755003","https://openalex.org/W2607153281","https://openalex.org/W2751197416","https://openalex.org/W2791013135","https://openalex.org/W2802611128","https://openalex.org/W2899814043","https://openalex.org/W2904898425","https://openalex.org/W2905272888","https://openalex.org/W2917177663","https://openalex.org/W4244425635","https://openalex.org/W4250903339","https://openalex.org/W4290144863","https://openalex.org/W6697398635"],"related_works":["https://openalex.org/W2390313118","https://openalex.org/W2046682228","https://openalex.org/W2162363184","https://openalex.org/W1991509461","https://openalex.org/W1413765351","https://openalex.org/W2766881779","https://openalex.org/W4395242122","https://openalex.org/W2491755398","https://openalex.org/W2905853915","https://openalex.org/W1789001211"],"abstract_inverted_index":{"To":[0],"systematically":[1],"analyze":[2],"biological":[3],"changes":[4,101],"with":[5,124],"spatiotemporal":[6],"dynamics,":[7],"it":[8],"is":[9,16],"important":[10,146],"to":[11,176,188],"establish":[12],"a":[13,45,50,72,119,148,174],"system":[14,46,77,123,172],"that":[15,61,92,142,163],"amenable":[17],"for":[18,49,95,128],"real-time":[19],"in":[20,36,54,56,102],"vivo":[21,55],"imaging":[22,76],"at":[23,106,131,203],"various":[24],"size":[25],"levels.":[26,136,206],"Herein,":[27],"we":[28,82],"focused":[29],"on":[30],"the":[31,37,62,79,87,103,107,132,178,195,204],"developing":[32],"pupal":[33,64],"wing":[34,65],"tissues":[35],"pale":[38],"grass":[39],"blue":[40],"butterfly,":[41],"Zizeeria":[42],"maha,":[43],"as":[44,147,194],"of":[47,86,150,181,197],"choice":[48],"systematic":[51],"multiscale":[52,205],"approach":[53],"real":[57],"time.":[58],"We":[59,98,116,137,155],"showed":[60],"entire":[63],"could":[66],"be":[67,144,186],"monitored":[68,100],"throughout":[69],"development":[70],"using":[71],"high-resolution":[73],"bright-field":[74],"time-lapse":[75],"under":[78],"forewing-lift":[80],"configuration;":[81],"recorded":[83],"detailed":[84],"dynamics":[85],"dorsal":[88,104],"and":[89,110,134,153,160,184,200],"ventral":[90],"epithelia":[91],"behaved":[93],"independently":[94],"peripheral":[96],"adjustment.":[97],"also":[99,117,156],"hindwing":[105],"compartmental":[108],"level":[109],"directly":[111],"observed":[112],"evaginating":[113],"scale":[114,201],"buds.":[115],"employed":[118],"confocal":[120],"laser":[121],"microscopy":[122],"multiple":[125],"fluorescent":[126],"dyes":[127],"three-dimensional":[129],"observations":[130],"tissue":[133],"cellular":[135,140,151],"discovered":[138],"extensive":[139],"clusters":[141],"may":[143,164],"functionally":[145],"unit":[149],"communication":[152],"differentiation.":[154],"identified":[157],"epithelial":[158,182],"discal":[159],"marginal":[161],"dents":[162],"function":[165],"during":[166],"development.":[167],"Together,":[168],"this":[169],"lycaenid":[170],"forewing":[171],"established":[173],"foundation":[175],"study":[177,189],"differentiation":[179],"process":[180],"cells":[183],"can":[185],"used":[187],"biophysically":[190],"challenging":[191],"mechanisms":[192],"such":[193],"determination":[196],"color":[198],"patterns":[199],"nanoarchitecture":[202]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":1}],"updated_date":"2026-05-21T09:19:25.381259","created_date":"2019-04-01T00:00:00"}