{"id":"https://openalex.org/W4405652550","doi":"https://doi.org/10.3389/fbinf.2024.1483255","title":"PRONAME: a user-friendly pipeline to process long-read nanopore metabarcoding data by generating high-quality consensus sequences","display_name":"PRONAME: a user-friendly pipeline to process long-read nanopore metabarcoding data by generating high-quality consensus sequences","publication_year":2024,"publication_date":"2024-12-20","ids":{"openalex":"https://openalex.org/W4405652550","doi":"https://doi.org/10.3389/fbinf.2024.1483255","pmid":"https://pubmed.ncbi.nlm.nih.gov/39758955"},"language":"en","primary_location":{"id":"doi:10.3389/fbinf.2024.1483255","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbinf.2024.1483255","pdf_url":null,"source":{"id":"https://openalex.org/S4210219554","display_name":"Frontiers in Bioinformatics","issn_l":"2673-7647","issn":["2673-7647"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3389/fbinf.2024.1483255","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046496120","display_name":"B. Dubois","orcid":"https://orcid.org/0000-0001-7891-0272"},"institutions":[{"id":"https://openalex.org/I4210101285","display_name":"Walloon Agricultural Research Centre","ror":"https://ror.org/016n74679","country_code":"BE","type":"facility","lineage":["https://openalex.org/I4210101285"]}],"countries":["BE"],"is_corresponding":true,"raw_author_name":"Benjamin Dubois","raw_affiliation_strings":["Bioengineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, Gembloux, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioengineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, Gembloux, Belgium","institution_ids":["https://openalex.org/I4210101285"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049449347","display_name":"Mathieu Delitte","orcid":null},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Mathieu Delitte","raw_affiliation_strings":["Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":[]},{"raw_affiliation_string":"Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":["https://openalex.org/I95674353"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094163510","display_name":"Salom\u00e9 Lengrand","orcid":null},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Salom\u00e9 Lengrand","raw_affiliation_strings":["Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":[]},{"raw_affiliation_string":"Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":["https://openalex.org/I95674353"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038916235","display_name":"Claude Bragard","orcid":"https://orcid.org/0000-0003-3169-1716"},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Claude Bragard","raw_affiliation_strings":["Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":[]},{"raw_affiliation_string":"Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":["https://openalex.org/I95674353"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044516253","display_name":"Anne Legr\u00e8ve","orcid":"https://orcid.org/0000-0002-7795-4510"},"institutions":[{"id":"https://openalex.org/I95674353","display_name":"UCLouvain","ror":"https://ror.org/02495e989","country_code":"BE","type":"education","lineage":["https://openalex.org/I95674353"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Anne Legr\u00e8ve","raw_affiliation_strings":["Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Earth and Life Institute - Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":[]},{"raw_affiliation_string":"Earth and Life Institute \u2013 Applied Microbiology, Plant Health, UCLouvain, Louvain-la-Neuve, Belgium","institution_ids":["https://openalex.org/I95674353"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5074732952","display_name":"Fr\u00e9d\u00e9ric Debode","orcid":"https://orcid.org/0000-0001-8733-4465"},"institutions":[{"id":"https://openalex.org/I4210101285","display_name":"Walloon Agricultural Research Centre","ror":"https://ror.org/016n74679","country_code":"BE","type":"facility","lineage":["https://openalex.org/I4210101285"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Fr\u00e9d\u00e9ric Debode","raw_affiliation_strings":["Bioengineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, Gembloux, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioengineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, Gembloux, Belgium","institution_ids":["https://openalex.org/I4210101285"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5046496120"],"corresponding_institution_ids":["https://openalex.org/I4210101285"],"apc_list":{"value":1900,"currency":"USD","value_usd":1900},"apc_paid":{"value":1900,"currency":"USD","value_usd":1900},"fwci":5.3455,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.95772604,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"4","issue":null,"first_page":"1483255","last_page":"1483255"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.37560001015663147,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.37560001015663147,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.2709999978542328,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.11569999903440475,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pipeline","display_name":"Pipeline (software)","score":0.7808576822280884},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.7045423984527588},{"id":"https://openalex.org/keywords/nanopore-sequencing","display_name":"Nanopore sequencing","score":0.5642161965370178},{"id":"https://openalex.org/keywords/nanopore","display_name":"Nanopore","score":0.5606649518013},{"id":"https://openalex.org/keywords/process","display_name":"Process (computing)","score":0.5131614804267883},{"id":"https://openalex.org/keywords/quality","display_name":"Quality (philosophy)","score":0.4721648395061493},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.35765254497528076},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.21655097603797913},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.1373814046382904},{"id":"https://openalex.org/keywords/operating-system","display_name":"Operating system","score":0.11617356538772583},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.11008194088935852},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.09308719635009766},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.07693833112716675},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.05950230360031128},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.05551505088806152}],"concepts":[{"id":"https://openalex.org/C43521106","wikidata":"https://www.wikidata.org/wiki/Q2165493","display_name":"Pipeline (software)","level":2,"score":0.7808576822280884},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.7045423984527588},{"id":"https://openalex.org/C126513998","wikidata":"https://www.wikidata.org/wiki/Q8973627","display_name":"Nanopore sequencing","level":4,"score":0.5642161965370178},{"id":"https://openalex.org/C141795571","wikidata":"https://www.wikidata.org/wiki/Q580942","display_name":"Nanopore","level":2,"score":0.5606649518013},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.5131614804267883},{"id":"https://openalex.org/C2779530757","wikidata":"https://www.wikidata.org/wiki/Q1207505","display_name":"Quality (philosophy)","level":2,"score":0.4721648395061493},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35765254497528076},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21655097603797913},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.1373814046382904},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.11617356538772583},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.11008194088935852},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.09308719635009766},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07693833112716675},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.05950230360031128},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.05551505088806152},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.3389/fbinf.2024.1483255","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbinf.2024.1483255","pdf_url":null,"source":{"id":"https://openalex.org/S4210219554","display_name":"Frontiers in Bioinformatics","issn_l":"2673-7647","issn":["2673-7647"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioinformatics","raw_type":"journal-article"},{"id":"pmid:39758955","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39758955","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in bioinformatics","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:11695402","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11695402","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Front Bioinform","raw_type":"Text"},{"id":"pmh:oai:dial.uclouvain.be:boreal:296069","is_oa":true,"landing_page_url":"http://hdl.handle.net/","pdf_url":null,"source":{"id":"https://openalex.org/S4306401902","display_name":"Digital Access to Libraries (Universit\u00e9 catholique de Louvain (UCL), l'Universit\u00e9 de Namur (UNamur) and the Universit\u00e9 Saint-Louis (USL-B))","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I95674353","host_organization_name":"UCLouvain","host_organization_lineage":["https://openalex.org/I95674353"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in bioinformatics, Vol. 4, p. 1-13 (2024)","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:2de8bbc4e5474d22bfee8845e2346f6e","is_oa":true,"landing_page_url":"https://doaj.org/article/2de8bbc4e5474d22bfee8845e2346f6e","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Bioinformatics, Vol 4 (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fbinf.2024.1483255","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fbinf.2024.1483255","pdf_url":null,"source":{"id":"https://openalex.org/S4210219554","display_name":"Frontiers in Bioinformatics","issn_l":"2673-7647","issn":["2673-7647"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W346827628","https://openalex.org/W2007772656","https://openalex.org/W2031050749","https://openalex.org/W2034285706","https://openalex.org/W2036897871","https://openalex.org/W2056279562","https://openalex.org/W2092784580","https://openalex.org/W2099901798","https://openalex.org/W2142678478","https://openalex.org/W2194172909","https://openalex.org/W2195724570","https://openalex.org/W2346680365","https://openalex.org/W2396849069","https://openalex.org/W2513506562","https://openalex.org/W2529838505","https://openalex.org/W2582743722","https://openalex.org/W2788228074","https://openalex.org/W2906533779","https://openalex.org/W2909919296","https://openalex.org/W2940820737","https://openalex.org/W2946022843","https://openalex.org/W2951278111","https://openalex.org/W2963276645","https://openalex.org/W2966597946","https://openalex.org/W2985711816","https://openalex.org/W2994969834","https://openalex.org/W3007172120","https://openalex.org/W3007601977","https://openalex.org/W3038869268","https://openalex.org/W3115745540","https://openalex.org/W3120817351","https://openalex.org/W3121375113","https://openalex.org/W3123501160","https://openalex.org/W3128892236","https://openalex.org/W3164465449","https://openalex.org/W3194252622","https://openalex.org/W3199385470","https://openalex.org/W4210525127","https://openalex.org/W4220926871","https://openalex.org/W4246311412","https://openalex.org/W4284896338","https://openalex.org/W4289203378","https://openalex.org/W4323667223","https://openalex.org/W4353066319","https://openalex.org/W4360826396","https://openalex.org/W4368377691","https://openalex.org/W4378783688","https://openalex.org/W4380988183","https://openalex.org/W4385068503","https://openalex.org/W4385302437","https://openalex.org/W4387906854","https://openalex.org/W4399429896","https://openalex.org/W4404511471","https://openalex.org/W6629479966"],"related_works":["https://openalex.org/W2774675543","https://openalex.org/W2119052929","https://openalex.org/W2326282944","https://openalex.org/W2330402197","https://openalex.org/W2420153309","https://openalex.org/W3201371206","https://openalex.org/W3175842380","https://openalex.org/W2096970964","https://openalex.org/W2107323267","https://openalex.org/W2589591413"],"abstract_inverted_index":{"Background:":[0],"The":[1,159,181],"study":[2],"of":[3,22,33,38,100,173,179,194,236,245,276,282,288,291],"sample":[4],"taxonomic":[5,74],"composition":[6],"has":[7],"evolved":[8],"from":[9],"direct":[10],"observations":[11],"and":[12,41,64,93,105,144,146,150,191,253,264],"labor-intensive":[13],"morphological":[14],"studies":[15,24],"to":[16,69,111,154,199,226,280],"different":[17],"DNA":[18],"sequencing":[19,49,78,133,197,278],"methodologies.":[20],"Most":[21],"these":[23],"leverage":[25],"the":[26,31,39,58,98,171,195,243,274,289],"metabarcoding":[27,174],"approach,":[28],"which":[29],"involves":[30],"amplification":[32],"a":[34,70,81,85,147,164,206,230],"small":[35],"taxonomically-informative":[36],"portion":[37],"genome":[40],"its":[42],"subsequent":[43],"high-throughput":[44],"sequencing.":[45],"Recent":[46],"advances":[47],"in":[48,73],"technology":[50],"brought":[51],"by":[52],"Oxford":[53],"Nanopore":[54,77,132,247,277],"Technologies":[55],"have":[56,209],"revolutionized":[57],"field,":[59],"enabling":[60,170],"portability,":[61],"affordable":[62],"cost":[63],"long-read":[65,114,246,292],"sequencing,":[66,92,284],"therefore":[67,169],"leading":[68],"significant":[71],"increase":[72],"resolution.":[75],"However,":[76],"data":[79,102,175,248],"exhibit":[80],"particular":[82],"profile,":[83],"with":[84,90,221],"higher":[86],"error":[87,265],"rate":[88],"compared":[89],"Illumina":[91,283],"existing":[94,256],"bioinformatics":[95],"pipelines":[96],"for":[97,129,139,176,233],"analysis":[99,172,235],"such":[101],"are":[103],"scarce":[104],"often":[106],"insufficient,":[107],"requiring":[108],"specialized":[109],"tools":[110],"accurately":[112],"process":[113],"sequences.":[115,158,271],"Results:":[116],"We":[117],"present":[118],"PRONAME":[119,135,212,241,267],"(PROcessing":[120],"NAnopore":[121],"MEtabarcoding":[122],"data),":[123],"an":[124],"open-source,":[125],"user-friendly":[126],"pipeline":[127,182],"optimized":[128],"processing":[130],"raw":[131],"data.":[134],"includes":[136],"precompiled":[137],"databases":[138],"complete":[140],"16S":[141],"sequences":[142,215],"(Silva138":[143],"Greengenes2)":[145],"newly":[148],"developed":[149],"curated":[151],"database":[152,166],"dedicated":[153],"bacterial":[155],"16S-ITS-23S":[156],"operon":[157],"user":[160],"can":[161],"also":[162],"provide":[163],"custom":[165],"if":[167],"desired,":[168],"any":[177],"domain":[178],"life.":[180],"significantly":[183],"improves":[184],"sequence":[185],"accuracy,":[186],"implementing":[187],"innovative":[188],"error-correction":[189],"strategies":[190],"taking":[192,286],"advantage":[193,287],"new":[196],"chemistry":[198],"produce":[200],"high-quality":[201],"duplex":[202],"reads.":[203],"Evaluations":[204],"using":[205],"mock":[207],"community":[208],"shown":[210],"that":[211,281],"delivers":[213],"consensus":[214,270],"demonstrating":[216],"at":[217],"least":[218],"99.5%":[219],"accuracy":[220,252,275],"standard":[222],"settings":[223],"(and":[224],"up":[225],"99.7%),":[227],"making":[228],"it":[229],"robust":[231],"tool":[232],"genomic":[234],"complex":[237],"multi-species":[238],"communities.":[239],"Conclusion:":[240],"meets":[242],"challenges":[244],"processing,":[249],"offering":[250],"greater":[251],"versatility":[254],"than":[255],"pipelines.":[257],"By":[258],"integrating":[259],"Nanopore-specific":[260],"quality":[261],"filtering,":[262],"clustering":[263],"correction,":[266],"produces":[268],"high-precision":[269],"This":[272],"brings":[273],"close":[279],"while":[285],"benefits":[290],"technologies.":[293]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":9}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}