{"id":"https://openalex.org/W4300305017","doi":"https://doi.org/10.2390/biecoll-jib-2015-283","title":"Online High-throughput Mutagenesis Designer Using Scoring Matrix of Sequence-specific Endonucleases","display_name":"Online High-throughput Mutagenesis Designer Using Scoring Matrix of Sequence-specific Endonucleases","publication_year":2015,"publication_date":"2015-01-01","ids":{"openalex":"https://openalex.org/W4300305017","doi":"https://doi.org/10.2390/biecoll-jib-2015-283"},"language":"en","primary_location":{"id":"pmh:oai:doaj.org/article:1c69127795cb4389802c42780763922b","is_oa":true,"landing_page_url":"https://doaj.org/article/1c69127795cb4389802c42780763922b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Integrative Bioinformatics, Vol 12, Iss 1, Pp 35-48 (2015)","raw_type":"article"},"type":"article","indexed_in":["datacite","doaj"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doaj.org/article/1c69127795cb4389802c42780763922b","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101300916","display_name":"Dayong Guo","orcid":null},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Guo, Dayong","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100383712","display_name":"Xiaojing Li","orcid":"https://orcid.org/0000-0003-0454-5916"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Li, Xiaojing","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100583076","display_name":"Pan Zhu","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhu, Pan","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101125495","display_name":"Yanrong Feng","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Feng, Yanrong","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103666143","display_name":"Yang Juan","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yang, Juan","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057855424","display_name":"Zhihong Zheng","orcid":null},"institutions":[{"id":"https://openalex.org/I91656880","display_name":"China Medical University","ror":"https://ror.org/032d4f246","country_code":"CN","type":"education","lineage":["https://openalex.org/I91656880"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zheng, Zhihong","raw_affiliation_strings":["Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China","institution_ids":["https://openalex.org/I91656880"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103985828","display_name":"Yang Wei","orcid":null},"institutions":[{"id":"https://openalex.org/I91656880","display_name":"China Medical University","ror":"https://ror.org/032d4f246","country_code":"CN","type":"education","lineage":["https://openalex.org/I91656880"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yang, Wei","raw_affiliation_strings":["Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China","institution_ids":["https://openalex.org/I91656880"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001215328","display_name":"Zhang Enuo","orcid":null},"institutions":[{"id":"https://openalex.org/I91656880","display_name":"China Medical University","ror":"https://ror.org/032d4f246","country_code":"CN","type":"education","lineage":["https://openalex.org/I91656880"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhang, Enuo","raw_affiliation_strings":["Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China","institution_ids":["https://openalex.org/I91656880"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yu, Yang","orcid":null},"institutions":[{"id":"https://openalex.org/I91656880","display_name":"China Medical University","ror":"https://ror.org/032d4f246","country_code":"CN","type":"education","lineage":["https://openalex.org/I91656880"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yu, Yang","raw_affiliation_strings":["Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratory of Transgenic Animal Research, Laboratory Animal Center of Liaoning Province, China Medical University, Shenyang, China","institution_ids":["https://openalex.org/I91656880"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Zhou, Shenglai","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhou, Shenglai","raw_affiliation_strings":["Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Syncbiotech Co. Ltd, 12 Xuefu Rd, High-tech District, Nanjing, China China","institution_ids":[]}]},{"author_position":"last","author":{"id":null,"display_name":"Wang, Hongyu","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wang, Hongyu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":11,"corresponding_author_ids":["https://openalex.org/A5101300916"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3012,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.64381176,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9628000259399414,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9628000259399414,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9623000025749207,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mutagenesis","display_name":"Mutagenesis","score":0.5648534297943115},{"id":"https://openalex.org/keywords/throughput","display_name":"Throughput","score":0.5612558722496033},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5400373339653015},{"id":"https://openalex.org/keywords/matrix","display_name":"Matrix (chemical analysis)","score":0.5319573879241943},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5290732979774475},{"id":"https://openalex.org/keywords/sequence","display_name":"Sequence (biology)","score":0.45979899168014526},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.3041050434112549},{"id":"https://openalex.org/keywords/mutation","display_name":"Mutation","score":0.2812139391899109},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.22371196746826172},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.15198037028312683},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.12173232436180115},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.11773338913917542},{"id":"https://openalex.org/keywords/operating-system","display_name":"Operating system","score":0.06503298878669739}],"concepts":[{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5648534297943115},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.5612558722496033},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5400373339653015},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.5319573879241943},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5290732979774475},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.45979899168014526},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3041050434112549},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.2812139391899109},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22371196746826172},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.15198037028312683},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12173232436180115},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.11773338913917542},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.06503298878669739},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:doaj.org/article:1c69127795cb4389802c42780763922b","is_oa":true,"landing_page_url":"https://doaj.org/article/1c69127795cb4389802c42780763922b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Integrative Bioinformatics, Vol 12, Iss 1, Pp 35-48 (2015)","raw_type":"article"},{"id":"doi:10.2390/biecoll-jib-2015-283","is_oa":true,"landing_page_url":"https://doi.org/10.2390/biecoll-jib-2015-283","pdf_url":null,"source":{"id":"https://openalex.org/S4306401670","display_name":"PUB \u2013 Publications at Bielefeld University (Bielefeld University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I20121455","host_organization_name":"Bielefeld University","host_organization_lineage":["https://openalex.org/I20121455"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"pmh:oai:doaj.org/article:1c69127795cb4389802c42780763922b","is_oa":true,"landing_page_url":"https://doaj.org/article/1c69127795cb4389802c42780763922b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Integrative Bioinformatics, Vol 12, Iss 1, Pp 35-48 (2015)","raw_type":"article"},"sustainable_development_goals":[{"score":0.4699999988079071,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1576852562","https://openalex.org/W3141735581","https://openalex.org/W2136583354","https://openalex.org/W191244194","https://openalex.org/W2111238207","https://openalex.org/W2386140904","https://openalex.org/W2760721665","https://openalex.org/W1827023575","https://openalex.org/W4388384997","https://openalex.org/W2411516357"],"abstract_inverted_index":{"CRISPR":[0],"Cas9":[1],"and":[2,13,18],"other":[3],"sequence-specific":[4],"endonucleases":[5],"are":[6],"fundamental":[7],"genome":[8],"editors":[9],"supporting":[10],"gene":[11,14,29],"knockout":[12],"therapy.":[15],"A":[16],"speedy":[17],"accurate":[19],"computational":[20],"allele":[21],"designer":[22],"is":[23],"required":[24],"for":[25],"a":[26],"high":[27],"through-put":[28],"mutagenesis":[30],"pipeline":[31],"using":[32],"these":[33],"new":[34],"techniques.":[35]},"counts_by_year":[{"year":2017,"cited_by_count":1}],"updated_date":"2026-05-06T08:25:59.206177","created_date":"2025-10-10T00:00:00"}