{"id":"https://openalex.org/W2096359134","doi":"https://doi.org/10.1371/journal.pcbi.1002171","title":"High-Precision, In Vitro Validation of the Sequestration Mechanism for Generating Ultrasensitive Dose-Response Curves in Regulatory Networks","display_name":"High-Precision, In Vitro Validation of the Sequestration Mechanism for Generating Ultrasensitive Dose-Response Curves in Regulatory Networks","publication_year":2011,"publication_date":"2011-10-06","ids":{"openalex":"https://openalex.org/W2096359134","doi":"https://doi.org/10.1371/journal.pcbi.1002171","mag":"2096359134","pmid":"https://pubmed.ncbi.nlm.nih.gov/21998566"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pcbi.1002171","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1002171","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002171&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS Computational Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002171&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018935401","display_name":"Francesco Ricci","orcid":"https://orcid.org/0000-0003-4941-8646"},"institutions":[{"id":"https://openalex.org/I154570441","display_name":"University of California, Santa Barbara","ror":"https://ror.org/02t274463","country_code":"US","type":"education","lineage":["https://openalex.org/I154570441"]},{"id":"https://openalex.org/I4210140048","display_name":"Istituto Nazionale Biostrutture e Biosistemi","ror":"https://ror.org/043bhwh19","country_code":"IT","type":"other","lineage":["https://openalex.org/I4210140048"]},{"id":"https://openalex.org/I116067653","display_name":"University of Rome Tor Vergata","ror":"https://ror.org/02p77k626","country_code":"IT","type":"education","lineage":["https://openalex.org/I116067653"]}],"countries":["IT","US"],"is_corresponding":false,"raw_author_name":"Francesco Ricci","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America","Dipartimento di Scienze e Tecnologie Chimiche, University of Rome, Tor Vergata, Rome, Italy","Consorzio Interuniversitario Biostrutture e Biosistemi \"INBB\", Rome, Italy"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America","institution_ids":["https://openalex.org/I154570441"]},{"raw_affiliation_string":"Dipartimento di Scienze e Tecnologie Chimiche, University of Rome, Tor Vergata, Rome, Italy","institution_ids":["https://openalex.org/I116067653"]},{"raw_affiliation_string":"Consorzio Interuniversitario Biostrutture e Biosistemi \"INBB\", Rome, Italy","institution_ids":["https://openalex.org/I4210140048"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040405132","display_name":"Alexis Vall\u00e9e\u2010B\u00e9lisle","orcid":"https://orcid.org/0000-0002-5009-7715"},"institutions":[{"id":"https://openalex.org/I154570441","display_name":"University of California, Santa Barbara","ror":"https://ror.org/02t274463","country_code":"US","type":"education","lineage":["https://openalex.org/I154570441"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexis Vall\u00e9e-B\u00e9lisle","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America","institution_ids":["https://openalex.org/I154570441"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067240770","display_name":"Kevin W. Plaxco","orcid":"https://orcid.org/0000-0003-4772-8771"},"institutions":[{"id":"https://openalex.org/I154570441","display_name":"University of California, Santa Barbara","ror":"https://ror.org/02t274463","country_code":"US","type":"education","lineage":["https://openalex.org/I154570441"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kevin W. Plaxco","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America","Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, California, United States of America"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of California, Santa Barbara, California, United States of America","institution_ids":["https://openalex.org/I154570441"]},{"raw_affiliation_string":"Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, California, United States of America","institution_ids":["https://openalex.org/I154570441"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5067240770"],"corresponding_institution_ids":["https://openalex.org/I154570441"],"apc_list":{"value":2655,"currency":"USD","value_usd":2655},"apc_paid":{"value":2655,"currency":"USD","value_usd":2655},"fwci":2.5346,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.89769094,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":"7","issue":"10","first_page":"e1002171","last_page":"e1002171"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9957000017166138,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9936000108718872,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/molecular-beacon","display_name":"Molecular beacon","score":0.6979079246520996},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.5574281811714172},{"id":"https://openalex.org/keywords/mechanism","display_name":"Mechanism (biology)","score":0.5486605167388916},{"id":"https://openalex.org/keywords/sensitivity","display_name":"Sensitivity (control systems)","score":0.48434460163116455},{"id":"https://openalex.org/keywords/occupancy","display_name":"Occupancy","score":0.44838830828666687},{"id":"https://openalex.org/keywords/biophysics","display_name":"Biophysics","score":0.44089967012405396},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.41727614402770996},{"id":"https://openalex.org/keywords/biochemical-engineering","display_name":"Biochemical engineering","score":0.4054843783378601},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.37451744079589844},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.3471069037914276},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.2775096893310547},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.18741676211357117},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.18554478883743286},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.1423722207546234},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.13311713933944702},{"id":"https://openalex.org/keywords/ecology","display_name":"Ecology","score":0.09437191486358643}],"concepts":[{"id":"https://openalex.org/C119203544","wikidata":"https://www.wikidata.org/wiki/Q425891","display_name":"Molecular beacon","level":4,"score":0.6979079246520996},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.5574281811714172},{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.5486605167388916},{"id":"https://openalex.org/C21200559","wikidata":"https://www.wikidata.org/wiki/Q7451068","display_name":"Sensitivity (control systems)","level":2,"score":0.48434460163116455},{"id":"https://openalex.org/C160331591","wikidata":"https://www.wikidata.org/wiki/Q7075743","display_name":"Occupancy","level":2,"score":0.44838830828666687},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.44089967012405396},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.41727614402770996},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.4054843783378601},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37451744079589844},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3471069037914276},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2775096893310547},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.18741676211357117},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.18554478883743286},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1423722207546234},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.13311713933944702},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.09437191486358643},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.0},{"id":"https://openalex.org/C24326235","wikidata":"https://www.wikidata.org/wiki/Q126095","display_name":"Electronic engineering","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053263","descriptor_name":"Gene Regulatory Networks","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053263","descriptor_name":"Gene Regulatory Networks","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053263","descriptor_name":"Gene Regulatory Networks","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1371/journal.pcbi.1002171","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1002171","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002171&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS Computational Biology","raw_type":"journal-article"},{"id":"pmid:21998566","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21998566","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS computational biology","raw_type":null},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.290.653","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.290.653","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/7f/67/PLoS_Comput_Biol_2011_Oct_6_7(10)_e1002171.tar.gz","raw_type":"text"},{"id":"pmh:oai:art.torvergata.it:2108/41732","is_oa":false,"landing_page_url":"http://hdl.handle.net/2108/41732","pdf_url":null,"source":{"id":"https://openalex.org/S4306400993","display_name":"Cineca Institutional Research Information System (Tor Vergata University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I116067653","host_organization_name":"University of Rome Tor Vergata","host_organization_lineage":["https://openalex.org/I116067653"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:74f97a214d3b4fcba00d7e72fcfed928","is_oa":true,"landing_page_url":"https://doaj.org/article/74f97a214d3b4fcba00d7e72fcfed928","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS Computational Biology, Vol 7, Iss 10, p e1002171 (2011)","raw_type":"article"},{"id":"pmh:oai:europepmc.org:2195567","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3188500","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:figshare.com:article/132596","is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/High_Precision_In_Vitro_Validation_of_the_Sequestration_Mechanism_for_Generating_Ultrasensitive_Dose_Response_Curves_in_Regulatory_Networks/132596","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pcbi.1002171","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1002171","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1002171&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS Computational Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G3065192081","display_name":null,"funder_award_id":"EB00768","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G8091430692","display_name":null,"funder_award_id":"(MIUR)","funder_id":"https://openalex.org/F4320321873","funder_display_name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca"}],"funders":[{"id":"https://openalex.org/F4320321873","display_name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","ror":"https://ror.org/0166hxq48"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2096359134.pdf","grobid_xml":"https://content.openalex.org/works/W2096359134.grobid-xml"},"referenced_works_count":48,"referenced_works":["https://openalex.org/W1483093256","https://openalex.org/W1492299013","https://openalex.org/W1530371171","https://openalex.org/W1544401280","https://openalex.org/W1557270760","https://openalex.org/W1645305843","https://openalex.org/W1671879096","https://openalex.org/W1967660626","https://openalex.org/W1969644422","https://openalex.org/W1977312644","https://openalex.org/W1985047295","https://openalex.org/W1990410909","https://openalex.org/W1991095439","https://openalex.org/W1998318331","https://openalex.org/W2021099440","https://openalex.org/W2021209514","https://openalex.org/W2025544995","https://openalex.org/W2026710381","https://openalex.org/W2047849624","https://openalex.org/W2057993765","https://openalex.org/W2069089909","https://openalex.org/W2076852768","https://openalex.org/W2077336113","https://openalex.org/W2082260955","https://openalex.org/W2092102609","https://openalex.org/W2100404753","https://openalex.org/W2104123948","https://openalex.org/W2107444209","https://openalex.org/W2109441358","https://openalex.org/W2111870329","https://openalex.org/W2127641960","https://openalex.org/W2128605063","https://openalex.org/W2132796188","https://openalex.org/W2134759932","https://openalex.org/W2135326887","https://openalex.org/W2137458512","https://openalex.org/W2137708006","https://openalex.org/W2137727595","https://openalex.org/W2139972745","https://openalex.org/W2141092850","https://openalex.org/W2144347639","https://openalex.org/W2147752484","https://openalex.org/W2161251399","https://openalex.org/W2164889880","https://openalex.org/W2165597714","https://openalex.org/W2169436912","https://openalex.org/W2172007697","https://openalex.org/W2586256972"],"related_works":["https://openalex.org/W4282043467","https://openalex.org/W2105697914","https://openalex.org/W2202433167","https://openalex.org/W3093197249","https://openalex.org/W1540010871","https://openalex.org/W3023979140","https://openalex.org/W3177545769","https://openalex.org/W2592350013","https://openalex.org/W2904068067","https://openalex.org/W4306842392"],"abstract_inverted_index":{"Our":[0],"ability":[1],"to":[2,25,48,74,122,133,148,171],"recreate":[3,75],"complex":[4],"biochemical":[5],"mechanisms":[6,20],"in":[7,28,39,130,191],"designed,":[8],"artificial":[9,29,224],"systems":[10,222],"provides":[11],"a":[12,68,111,157,178],"stringent":[13],"test":[14],"of":[15,18,54,58,85,95,110,138,145,151,177,185,194,219],"our":[16],"understanding":[17],"these":[19],"and":[21,79,93,97,223],"opens":[22],"the":[23,41,50,55,76,90,107,118,139,143,168,173,188,192,197,201,217],"door":[24],"their":[26],"exploitation":[27],"biotechnologies.":[30],"Motivated":[31],"by":[32,46,182,207],"this":[33,164,212],"philosophy,":[34],"here":[35],"we":[36,63,104,165,209],"have":[37,64,166],"recapitulated":[38],"vitro":[40],"\"target":[42],"sequestration\"":[43],"mechanism":[44,78,170,203],"used":[45],"nature":[47],"improve":[49,216],"sensitivity":[51,144,189],"(the":[52],"steepness":[53],"input/output":[56],"curve)":[57],"many":[59],"regulatory":[60],"cascades.":[61],"Specifically,":[62],"employed":[65,70,206],"molecular":[66,113,146],"beacons,":[67],"commonly":[69],"optical":[71],"DNA":[72],"sensor,":[73],"sequestration":[77,169,202],"performed":[80],"an":[81,127,152,183],"exhaustive,":[82],"quantitative":[83],"study":[84],"its":[86],"key":[87],"determinants":[88],"(e.g.,":[89],"relative":[91],"concentrations":[92],"affinities":[94],"probe":[96],"depletant).":[98],"We":[99],"show":[100],"that,":[101],"using":[102],"sequestration,":[103],"can":[105],"narrow":[106],"pseudo-linear":[108],"range":[109,141],"traditional":[112],"beacon":[114],"from":[115,120],"81-fold":[116,128],"(i.e.,":[117],"transition":[119],"10%":[121],"90%":[123],"target":[124,131],"occupancy":[125,175],"spans":[126],"change":[129],"concentration)":[132],"just":[134],"1.5-fold.":[135],"This":[136],"narrowing":[137],"dynamic":[140],"improves":[142],"beacons":[147],"that":[149,211],"equivalent":[150],"oligomeric,":[153],"allosteric":[154],"receptor":[155],"with":[156,199],"Hill":[158],"coefficient":[159],"greater":[160],"than":[161],"9.":[162],"Following":[163],"adapted":[167],"steepen":[172],"binding-site":[174],"curve":[176],"common":[179],"transcription":[180],"factor":[181],"order":[184],"magnitude":[186],"over":[187],"observed":[190],"absence":[193],"sequestration.":[195],"Given":[196],"success":[198],"which":[200],"has":[204],"been":[205],"nature,":[208],"believe":[210],"strategy":[213],"could":[214],"dramatically":[215],"performance":[218],"synthetic":[220],"biological":[221],"biosensors.":[225]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":4}],"updated_date":"2026-04-10T15:06:20.359241","created_date":"2025-10-10T00:00:00"}