{"id":"https://openalex.org/W4417507904","doi":"https://doi.org/10.1371/journal.pcbi.1013819","title":"Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud","display_name":"Democratising high performance computing for bioinformatics through serverless cloud computing: A case study on CRISPR-Cas9 guide RNA design with Crackling Cloud","publication_year":2025,"publication_date":"2025-12-19","ids":{"openalex":"https://openalex.org/W4417507904","doi":"https://doi.org/10.1371/journal.pcbi.1013819","pmid":"https://pubmed.ncbi.nlm.nih.gov/41417859"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pcbi.1013819","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013819","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1013819&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1013819&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009487082","display_name":"Jacob Bradford","orcid":"https://orcid.org/0000-0003-3619-5682"},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Jacob Bradford","raw_affiliation_strings":["Centre for Data Science, Queensland University of Technology, Brisbane City, Queensland, Australia","School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":"https://orcid.org/0000-0003-3619-5682","affiliations":[{"raw_affiliation_string":"Centre for Data Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]},{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Divya Joy","orcid":null},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Divya Joy","raw_affiliation_strings":["School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115005069","display_name":"Mattias Winsen","orcid":null},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Mattias Winsen","raw_affiliation_strings":["School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115005070","display_name":"Nicholas Meurant","orcid":null},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Nicholas Meurant","raw_affiliation_strings":["School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Mackenzie Wilkins","orcid":null},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Mackenzie Wilkins","raw_affiliation_strings":["School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Laurence O.W. Wilson","orcid":null},"institutions":[{"id":"https://openalex.org/I1292875679","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07","country_code":"AU","type":"government","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801453606","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I4210141844","display_name":"Australian e-Health Research Centre","ror":"https://ror.org/04ywhbc61","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801244131","https://openalex.org/I2801453606","https://openalex.org/I4210141844","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I99043593","display_name":"Macquarie University","ror":"https://ror.org/01sf06y89","country_code":"AU","type":"education","lineage":["https://openalex.org/I99043593"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Laurence O.W. Wilson","raw_affiliation_strings":["Applied BioSciences, Faculty of Science and Engineering, Macquarie University, Macquarie Park, New South Wales, Australia","Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied BioSciences, Faculty of Science and Engineering, Macquarie University, Macquarie Park, New South Wales, Australia","institution_ids":["https://openalex.org/I99043593"]},{"raw_affiliation_string":"Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I1292875679","https://openalex.org/I4210141844"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107979977","display_name":"Denis C. Bauer","orcid":"https://orcid.org/0000-0001-8033-9810"},"institutions":[{"id":"https://openalex.org/I129604602","display_name":"The University of Sydney","ror":"https://ror.org/0384j8v12","country_code":"AU","type":"education","lineage":["https://openalex.org/I129604602"]},{"id":"https://openalex.org/I31746571","display_name":"UNSW Sydney","ror":"https://ror.org/03r8z3t63","country_code":"AU","type":"education","lineage":["https://openalex.org/I31746571"]},{"id":"https://openalex.org/I4210141844","display_name":"Australian e-Health Research Centre","ror":"https://ror.org/04ywhbc61","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1292875679","https://openalex.org/I2801244131","https://openalex.org/I2801453606","https://openalex.org/I4210141844","https://openalex.org/I4387156119"]},{"id":"https://openalex.org/I99043593","display_name":"Macquarie University","ror":"https://ror.org/01sf06y89","country_code":"AU","type":"education","lineage":["https://openalex.org/I99043593"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Denis C. Bauer","raw_affiliation_strings":["Applied BioSciences, Faculty of Science and Engineering, Macquarie University, Macquarie Park, New South Wales, Australia","Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Adelaide, South Australia, Australia","School of Medical Sciences, Department of Biomedical Informatics and Digital Health, University of Sydney, Sydney, New South Wales, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Applied BioSciences, Faculty of Science and Engineering, Macquarie University, Macquarie Park, New South Wales, Australia","institution_ids":["https://openalex.org/I99043593"]},{"raw_affiliation_string":"Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Adelaide, South Australia, Australia","institution_ids":["https://openalex.org/I4210141844"]},{"raw_affiliation_string":"School of Medical Sciences, Department of Biomedical Informatics and Digital Health, University of Sydney, Sydney, New South Wales, Australia","institution_ids":["https://openalex.org/I129604602","https://openalex.org/I31746571"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089156481","display_name":"Dimitri Perrin","orcid":"https://orcid.org/0000-0002-4007-5256"},"institutions":[{"id":"https://openalex.org/I160993911","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752","country_code":"AU","type":"education","lineage":["https://openalex.org/I160993911"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Dimitri Perrin","raw_affiliation_strings":["Centre for Data Science, Queensland University of Technology, Brisbane City, Queensland, Australia","School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre for Data Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]},{"raw_affiliation_string":"School of Computer Science, Faculty of Science, Queensland University of Technology, Brisbane City, Queensland, Australia","institution_ids":["https://openalex.org/I160993911"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5009487082","https://openalex.org/A5089156481"],"corresponding_institution_ids":["https://openalex.org/I160993911"],"apc_list":{"value":3043,"currency":"USD","value_usd":3043},"apc_paid":{"value":3043,"currency":"USD","value_usd":3043},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.22921142,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"12","first_page":"e1013819","last_page":"e1013819"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9277999997138977,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9277999997138977,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11986","display_name":"Scientific Computing and Data Management","score":0.007799999788403511,"subfield":{"id":"https://openalex.org/subfields/1802","display_name":"Information Systems and Management"},"field":{"id":"https://openalex.org/fields/18","display_name":"Decision Sciences"},"domain":{"id":"https://openalex.org/domains/2","display_name":"Social Sciences"}},{"id":"https://openalex.org/T10714","display_name":"Software-Defined Networks and 5G","score":0.005900000222027302,"subfield":{"id":"https://openalex.org/subfields/1705","display_name":"Computer Networks and Communications"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cloud-computing","display_name":"Cloud computing","score":0.9126999974250793},{"id":"https://openalex.org/keywords/scalability","display_name":"Scalability","score":0.6381999850273132},{"id":"https://openalex.org/keywords/adaptation","display_name":"Adaptation (eye)","score":0.46889999508857727},{"id":"https://openalex.org/keywords/software","display_name":"Software","score":0.4490000009536743},{"id":"https://openalex.org/keywords/supercomputer","display_name":"Supercomputer","score":0.44029998779296875},{"id":"https://openalex.org/keywords/cloud-testing","display_name":"Cloud testing","score":0.3199999928474426}],"concepts":[{"id":"https://openalex.org/C79974875","wikidata":"https://www.wikidata.org/wiki/Q483639","display_name":"Cloud computing","level":2,"score":0.9126999974250793},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.7479000091552734},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.6381999850273132},{"id":"https://openalex.org/C139807058","wikidata":"https://www.wikidata.org/wiki/Q352374","display_name":"Adaptation (eye)","level":2,"score":0.46889999508857727},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.4490000009536743},{"id":"https://openalex.org/C83283714","wikidata":"https://www.wikidata.org/wiki/Q121117","display_name":"Supercomputer","level":2,"score":0.44029998779296875},{"id":"https://openalex.org/C120314980","wikidata":"https://www.wikidata.org/wiki/Q180634","display_name":"Distributed computing","level":1,"score":0.4059000015258789},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.38830000162124634},{"id":"https://openalex.org/C2522767166","wikidata":"https://www.wikidata.org/wiki/Q2374463","display_name":"Data science","level":1,"score":0.32429999113082886},{"id":"https://openalex.org/C120115606","wikidata":"https://www.wikidata.org/wiki/Q5135723","display_name":"Cloud testing","level":4,"score":0.3199999928474426},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.30329999327659607},{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.29109999537467957},{"id":"https://openalex.org/C115903868","wikidata":"https://www.wikidata.org/wiki/Q80993","display_name":"Software engineering","level":1,"score":0.28619998693466187},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.2851000130176544},{"id":"https://openalex.org/C69131567","wikidata":"https://www.wikidata.org/wiki/Q2068215","display_name":"Structural bioinformatics","level":3,"score":0.2797999978065491},{"id":"https://openalex.org/C118643609","wikidata":"https://www.wikidata.org/wiki/Q189210","display_name":"Web application","level":2,"score":0.2506999969482422}],"mesh":[{"descriptor_ui":"D000067917","descriptor_name":"Cloud Computing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000067917","descriptor_name":"Cloud Computing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000067917","descriptor_name":"Cloud Computing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000067917","descriptor_name":"Cloud Computing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1371/journal.pcbi.1013819","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013819","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1013819&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},{"id":"pmid:41417859","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41417859","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS computational biology","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:ffec56504b9b4d119dc3488774e13425","is_oa":true,"landing_page_url":"https://doaj.org/article/ffec56504b9b4d119dc3488774e13425","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS Computational Biology, Vol 21, Iss 12, p e1013819 (2025)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12742744","is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12742744/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS Comput Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1371/journal.pcbi.1013819","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013819","pdf_url":"https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1013819&type=printable","source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G4668895115","display_name":"Safer gene editing tools for Australian livestock and biotech industries","funder_award_id":"DP210103401","funder_id":"https://openalex.org/F4320334704","funder_display_name":"Australian Research Council"}],"funders":[{"id":"https://openalex.org/F4320320386","display_name":"Commonwealth Scientific and Industrial Research Organisation","ror":"https://ror.org/03qn8fb07"},{"id":"https://openalex.org/F4320320983","display_name":"Queensland University of Technology","ror":"https://ror.org/03pnv4752"},{"id":"https://openalex.org/F4320334704","display_name":"Australian Research Council","ror":"https://ror.org/05mmh0f86"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4417507904.pdf","grobid_xml":"https://content.openalex.org/works/W4417507904.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W2050480410","https://openalex.org/W2283794411","https://openalex.org/W2794757234","https://openalex.org/W2913847081","https://openalex.org/W2964265365","https://openalex.org/W2970173547","https://openalex.org/W2972675127","https://openalex.org/W2973142253","https://openalex.org/W2997231488","https://openalex.org/W3036330109","https://openalex.org/W3102171738","https://openalex.org/W3178931483","https://openalex.org/W3196617666","https://openalex.org/W4281852142","https://openalex.org/W4282913281","https://openalex.org/W4376614239","https://openalex.org/W4388967998","https://openalex.org/W4406260142"],"related_works":[],"abstract_inverted_index":{"Organisations":[0],"are":[1],"challenged":[2],"when":[3],"meeting":[4],"the":[5,25,67,79,91,127,136,165],"computational":[6,140],"requirements":[7],"of":[8,27,71,90,134],"large-scale":[9,20,30],"bioinformatics":[10,61,76,143],"analyses":[11],"using":[12,107],"their":[13],"own":[14],"resources.":[15],"Cloud":[16,150],"computing":[17,54,74,105],"has":[18,55],"democratised":[19],"resources,":[21],"and":[22,40,102,126,144,158],"to":[23,82,131,138,154],"reduce":[24],"barriers":[26],"working":[28],"with":[29,119],"compute,":[31],"leading":[32,123],"cloud":[33,124],"vendors":[34],"offer":[35],"serverless":[36,53,73],"computing,":[37],"a":[38,99],"low-maintenance":[39],"low-cost":[41],"model":[42],"that":[43],"provides":[44],"ample":[45],"resources":[46],"for":[47,75,145],"highly":[48],"scalable":[49],"software":[50],"applications.":[51],"While":[52],"broad":[56],"use,":[57],"its":[58],"adoption":[59],"in":[60,142],"remains":[62],"poor.":[63],"Here,":[64],"we":[65],"demonstrate":[66],"most":[68],"extensive":[69],"use":[70],"high-performance":[72,104],"by":[77,111],"applying":[78],"available":[80,110,161],"technologies":[81,108,120],"CRISPR-Cas9":[83,146],"guide":[84],"RNA":[85],"(gRNA)":[86],"design.":[87,148],"Our":[88],"adaptation":[89],"established":[92],"gRNA":[93,147],"design":[94],"tool,":[95],"named":[96],"Crackling,":[97],"implements":[98],"novel,":[100],"cloud-native":[101],"serverless-based,":[103],"environment":[106],"made":[109],"Amazon":[112],"Web":[113],"Services":[114],"(AWS).":[115],"The":[116],"architecture,":[117],"compatible":[118],"from":[121],"all":[122],"vendors,":[125],"AWS":[128,156],"implementation,":[129],"contributes":[130],"an":[132],"effort":[133],"reducing":[135],"barrier":[137],"large":[139],"capacity":[141],"Crackling":[149],"can":[151],"be":[152],"deployed":[153],"any":[155],"account,":[157],"is":[159],"freely":[160],"on":[162],"GitHub":[163],"under":[164],"BSD":[166],"3-clause":[167],"license:":[168],"https://github.com/bmds-lab/Crackling-AWS.":[169]},"counts_by_year":[],"updated_date":"2026-08-27T14:10:00.468798","created_date":"2025-12-19T00:00:00"}