{"id":"https://openalex.org/W4416664127","doi":"https://doi.org/10.1371/journal.pcbi.1013682","title":"Splicing-aware scRNA-Seq resolution reveals execution-ready programs in effector Tregs","display_name":"Splicing-aware scRNA-Seq resolution reveals execution-ready programs in effector Tregs","publication_year":2025,"publication_date":"2025-11-10","ids":{"openalex":"https://openalex.org/W4416664127","doi":"https://doi.org/10.1371/journal.pcbi.1013682","pmid":"https://pubmed.ncbi.nlm.nih.gov/41212920"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pcbi.1013682","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013682","pdf_url":null,"source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1371/journal.pcbi.1013682","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019088814","display_name":"Daniil K. Lukyanov","orcid":"https://orcid.org/0000-0002-8037-4752"},"institutions":[{"id":"https://openalex.org/I337778094","display_name":"Pirogov Russian National Research Medical University","ror":"https://ror.org/018159086","country_code":"RU","type":"education","lineage":["https://openalex.org/I337778094","https://openalex.org/I4210117042"]},{"id":"https://openalex.org/I4210105571","display_name":"Institute of Bioorganic Chemistry","ror":"https://ror.org/01dg04253","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210105571","https://openalex.org/I4210129690"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["FR","RU"],"is_corresponding":true,"raw_author_name":"Daniil K. Lukyanov","raw_affiliation_strings":["Center for Molecular and Cellular Biology, Moscow, Russia","Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia"],"affiliations":[{"raw_affiliation_string":"Center for Molecular and Cellular Biology, Moscow, Russia","institution_ids":["https://openalex.org/I4210158086"]},{"raw_affiliation_string":"Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","institution_ids":["https://openalex.org/I4210105571"]},{"raw_affiliation_string":"Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia","institution_ids":["https://openalex.org/I337778094"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008945411","display_name":"Evgeniy S. Egorov","orcid":"https://orcid.org/0009-0005-3003-1267"},"institutions":[{"id":"https://openalex.org/I19880235","display_name":"Lomonosov Moscow State University","ror":"https://ror.org/010pmpe69","country_code":"RU","type":"education","lineage":["https://openalex.org/I19880235"]},{"id":"https://openalex.org/I4210105571","display_name":"Institute of Bioorganic Chemistry","ror":"https://ror.org/01dg04253","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210105571","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Evgeniy S. Egorov","raw_affiliation_strings":["Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia","Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia"],"affiliations":[{"raw_affiliation_string":"Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia","institution_ids":["https://openalex.org/I19880235"]},{"raw_affiliation_string":"Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","institution_ids":["https://openalex.org/I4210105571"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5095034143","display_name":"Valeriia Kriukova","orcid":"https://orcid.org/0000-0002-5168-6511"},"institutions":[{"id":"https://openalex.org/I32021983","display_name":"Christian-Albrechts-Universit\u00e4t zu Kiel","ror":"https://ror.org/04v76ef78","country_code":"DE","type":"education","lineage":["https://openalex.org/I32021983"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Valeriia V. Kriukova","raw_affiliation_strings":["Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany"],"affiliations":[{"raw_affiliation_string":"Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany","institution_ids":["https://openalex.org/I32021983"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072769349","display_name":"Denis Syrko","orcid":"https://orcid.org/0000-0002-3263-4230"},"institutions":[{"id":"https://openalex.org/I337778094","display_name":"Pirogov Russian National Research Medical University","ror":"https://ror.org/018159086","country_code":"RU","type":"education","lineage":["https://openalex.org/I337778094","https://openalex.org/I4210117042"]},{"id":"https://openalex.org/I4210105571","display_name":"Institute of Bioorganic Chemistry","ror":"https://ror.org/01dg04253","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210105571","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Denis Syrko","raw_affiliation_strings":["Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia"],"affiliations":[{"raw_affiliation_string":"Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","institution_ids":["https://openalex.org/I4210105571"]},{"raw_affiliation_string":"Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia","institution_ids":["https://openalex.org/I337778094"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062285061","display_name":"V. Kotliar","orcid":"https://orcid.org/0000-0002-7443-4042"},"institutions":[{"id":"https://openalex.org/I4210105571","display_name":"Institute of Bioorganic Chemistry","ror":"https://ror.org/01dg04253","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210105571","https://openalex.org/I4210129690"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Victor V. Kotliar","raw_affiliation_strings":["Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia"],"affiliations":[{"raw_affiliation_string":"Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","institution_ids":["https://openalex.org/I4210105571"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049518622","display_name":"Kristin Ladell","orcid":"https://orcid.org/0000-0002-9856-2938"},"institutions":[{"id":"https://openalex.org/I2800861664","display_name":"University Hospital of Wales","ror":"https://ror.org/04fgpet95","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1299209451","https://openalex.org/I2800861664","https://openalex.org/I4210144625"]},{"id":"https://openalex.org/I79510175","display_name":"Cardiff University","ror":"https://ror.org/03kk7td41","country_code":"GB","type":"education","lineage":["https://openalex.org/I79510175"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Kristin Ladell","raw_affiliation_strings":["Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","Systems Immunity Research Institute, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom"],"affiliations":[{"raw_affiliation_string":"Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","institution_ids":["https://openalex.org/I2800861664","https://openalex.org/I79510175"]},{"raw_affiliation_string":"Systems Immunity Research Institute, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","institution_ids":["https://openalex.org/I2800861664","https://openalex.org/I79510175"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100694592","display_name":"David A. Price","orcid":"https://orcid.org/0000-0001-9416-2737"},"institutions":[{"id":"https://openalex.org/I2800861664","display_name":"University Hospital of Wales","ror":"https://ror.org/04fgpet95","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I1299209451","https://openalex.org/I2800861664","https://openalex.org/I4210144625"]},{"id":"https://openalex.org/I79510175","display_name":"Cardiff University","ror":"https://ror.org/03kk7td41","country_code":"GB","type":"education","lineage":["https://openalex.org/I79510175"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"David A. Price","raw_affiliation_strings":["Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","Systems Immunity Research Institute, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom"],"affiliations":[{"raw_affiliation_string":"Division of Infection and Immunity, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","institution_ids":["https://openalex.org/I2800861664","https://openalex.org/I79510175"]},{"raw_affiliation_string":"Systems Immunity Research Institute, Cardiff University School of Medicine, University Hospital of Wales, Cardiff, United Kingdom","institution_ids":["https://openalex.org/I2800861664","https://openalex.org/I79510175"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073345578","display_name":"Andr\u00e9 Franke","orcid":"https://orcid.org/0000-0003-1530-5811"},"institutions":[{"id":"https://openalex.org/I32021983","display_name":"Christian-Albrechts-Universit\u00e4t zu Kiel","ror":"https://ror.org/04v76ef78","country_code":"DE","type":"education","lineage":["https://openalex.org/I32021983"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Andre Franke","raw_affiliation_strings":["Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany"],"affiliations":[{"raw_affiliation_string":"Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany","institution_ids":["https://openalex.org/I32021983"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060028352","display_name":"Dmitriy M. Chudakov","orcid":"https://orcid.org/0000-0003-0430-790X"},"institutions":[{"id":"https://openalex.org/I1310815967","display_name":"Central European Institute of Technology","ror":"https://ror.org/01p7k1986","country_code":"CZ","type":"education","lineage":["https://openalex.org/I1310815967"]},{"id":"https://openalex.org/I337778094","display_name":"Pirogov Russian National Research Medical University","ror":"https://ror.org/018159086","country_code":"RU","type":"education","lineage":["https://openalex.org/I337778094","https://openalex.org/I4210117042"]},{"id":"https://openalex.org/I4210105571","display_name":"Institute of Bioorganic Chemistry","ror":"https://ror.org/01dg04253","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210105571","https://openalex.org/I4210129690"]},{"id":"https://openalex.org/I4210158086","display_name":"Institut de Biologie Mol\u00e9culaire et Cellulaire","ror":"https://ror.org/05qpmg879","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4210158086","https://openalex.org/I68947357"]}],"countries":["CZ","FR","RU"],"is_corresponding":true,"raw_author_name":"Dmitry M. Chudakov","raw_affiliation_strings":["Abu Dhabi Stem Cell Center, Al Muntazah, United Arab Emirates","Center for Molecular and Cellular Biology, Moscow, Russia","Central European Institute of Technology, Brno, Czech Republic","Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia"],"affiliations":[{"raw_affiliation_string":"Abu Dhabi Stem Cell Center, Al Muntazah, United Arab Emirates","institution_ids":[]},{"raw_affiliation_string":"Center for Molecular and Cellular Biology, Moscow, Russia","institution_ids":["https://openalex.org/I4210158086"]},{"raw_affiliation_string":"Central European Institute of Technology, Brno, Czech Republic","institution_ids":["https://openalex.org/I1310815967"]},{"raw_affiliation_string":"Genomics of Adaptive Immunity Department, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia","institution_ids":["https://openalex.org/I4210105571"]},{"raw_affiliation_string":"Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia","institution_ids":["https://openalex.org/I337778094"]}]}],"institutions":[],"countries_distinct_count":5,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5008945411","https://openalex.org/A5019088814","https://openalex.org/A5049518622","https://openalex.org/A5060028352","https://openalex.org/A5062285061","https://openalex.org/A5072769349","https://openalex.org/A5073345578","https://openalex.org/A5095034143","https://openalex.org/A5100694592"],"corresponding_institution_ids":["https://openalex.org/I1310815967","https://openalex.org/I19880235","https://openalex.org/I2800861664","https://openalex.org/I32021983","https://openalex.org/I337778094","https://openalex.org/I4210105571","https://openalex.org/I4210158086","https://openalex.org/I79510175"],"apc_list":{"value":2655,"currency":"USD","value_usd":2655},"apc_paid":{"value":2655,"currency":"USD","value_usd":2655},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.31878462,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"11","first_page":"e1013682","last_page":"e1013682"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.939300000667572,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.939300000667572,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.04610000178217888,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-splicing","display_name":"RNA splicing","score":0.6620000004768372},{"id":"https://openalex.org/keywords/effector","display_name":"Effector","score":0.6158999800682068},{"id":"https://openalex.org/keywords/messenger-rna","display_name":"Messenger RNA","score":0.5871000289916992},{"id":"https://openalex.org/keywords/rna","display_name":"RNA","score":0.5002999901771545},{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"Alternative splicing","score":0.47909998893737793},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.424699991941452},{"id":"https://openalex.org/keywords/gene-expression","display_name":"Gene expression","score":0.4097000062465668},{"id":"https://openalex.org/keywords/transcription","display_name":"Transcription (linguistics)","score":0.39160001277923584},{"id":"https://openalex.org/keywords/curse-of-dimensionality","display_name":"Curse of dimensionality","score":0.3440999984741211}],"concepts":[{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.6620000004768372},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6549000144004822},{"id":"https://openalex.org/C51785407","wikidata":"https://www.wikidata.org/wiki/Q2627568","display_name":"Effector","level":2,"score":0.6158999800682068},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5871000289916992},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5002999901771545},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4968000054359436},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.47909998893737793},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4722999930381775},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.424699991941452},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4097000062465668},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.39160001277923584},{"id":"https://openalex.org/C111030470","wikidata":"https://www.wikidata.org/wiki/Q1430460","display_name":"Curse of dimensionality","level":2,"score":0.3440999984741211},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.33480000495910645},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33169999718666077},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.3296000063419342},{"id":"https://openalex.org/C36823959","wikidata":"https://www.wikidata.org/wiki/Q373027","display_name":"Exon","level":3,"score":0.32899999618530273},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3260999917984009},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.319599986076355},{"id":"https://openalex.org/C2776950831","wikidata":"https://www.wikidata.org/wiki/Q15635432","display_name":"Single-cell analysis","level":3,"score":0.2978000044822693},{"id":"https://openalex.org/C2780114586","wikidata":"https://www.wikidata.org/wiki/Q40260","display_name":"Cell nucleus","level":3,"score":0.2953000068664551},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.28369998931884766},{"id":"https://openalex.org/C94671646","wikidata":"https://www.wikidata.org/wiki/Q207551","display_name":"Intron","level":3,"score":0.2816999852657318},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2791000008583069},{"id":"https://openalex.org/C189014844","wikidata":"https://www.wikidata.org/wiki/Q189118","display_name":"Cell type","level":3,"score":0.2759000062942505},{"id":"https://openalex.org/C102063271","wikidata":"https://www.wikidata.org/wiki/Q7243183","display_name":"Precursor mRNA","level":5,"score":0.26669999957084656},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.2632000148296356},{"id":"https://openalex.org/C2780723820","wikidata":"https://www.wikidata.org/wiki/Q1934178","display_name":"Nucleus","level":2,"score":0.2581999897956848},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.2554999887943268}],"mesh":[{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000092386","descriptor_name":"Single-Cell Gene Expression Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000092386","descriptor_name":"Single-Cell Gene Expression Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000092386","descriptor_name":"Single-Cell Gene Expression Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012326","descriptor_name":"RNA Splicing","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012326","descriptor_name":"RNA Splicing","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012326","descriptor_name":"RNA Splicing","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050378","descriptor_name":"T-Lymphocytes, Regulatory","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D050378","descriptor_name":"T-Lymphocytes, Regulatory","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D050378","descriptor_name":"T-Lymphocytes, Regulatory","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051858","descriptor_name":"Forkhead Transcription Factors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1371/journal.pcbi.1013682","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013682","pdf_url":null,"source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},{"id":"pmid:41212920","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/41212920","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS computational biology","raw_type":null},{"id":"pmh:oai:https://orca.cardiff.ac.uk:182723","is_oa":false,"landing_page_url":null,"pdf_url":null,"source":{"id":"https://openalex.org/S4306401195","display_name":"ORCA Online Research @Cardiff (Cardiff University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I79510175","host_organization_name":"Cardiff University","host_organization_lineage":["https://openalex.org/I79510175"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},{"id":"pmh:oai:doaj.org/article:9f982b7076d84ab8812d5b644d3ef852","is_oa":true,"landing_page_url":"https://doaj.org/article/9f982b7076d84ab8812d5b644d3ef852","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS Computational Biology, Vol 21, Iss 11, p e1013682 (2025)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1371/journal.pcbi.1013682","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pcbi.1013682","pdf_url":null,"source":{"id":"https://openalex.org/S86033158","display_name":"PLoS Computational Biology","issn_l":"1553-734X","issn":["1553-734X","1553-7358"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS Computational Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G663090419","display_name":null,"funder_award_id":"25-75-30013","funder_id":"https://openalex.org/F4320324099","funder_display_name":"Russian Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320324099","display_name":"Russian Science Foundation","ror":"https://ror.org/03y2gwe85"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":74,"referenced_works":["https://openalex.org/W1541174147","https://openalex.org/W1583391226","https://openalex.org/W1972916221","https://openalex.org/W1977871412","https://openalex.org/W1980693461","https://openalex.org/W1990295999","https://openalex.org/W1991344211","https://openalex.org/W2002726823","https://openalex.org/W2021878934","https://openalex.org/W2050002679","https://openalex.org/W2053763669","https://openalex.org/W2062564388","https://openalex.org/W2076655821","https://openalex.org/W2085596919","https://openalex.org/W2097811455","https://openalex.org/W2100142435","https://openalex.org/W2106190040","https://openalex.org/W2126072190","https://openalex.org/W2127533496","https://openalex.org/W2148392989","https://openalex.org/W2158715209","https://openalex.org/W2160506199","https://openalex.org/W2191493147","https://openalex.org/W2267789790","https://openalex.org/W2294086086","https://openalex.org/W2396424530","https://openalex.org/W2400276917","https://openalex.org/W2401985889","https://openalex.org/W2534681704","https://openalex.org/W2560111197","https://openalex.org/W2743416243","https://openalex.org/W2759609596","https://openalex.org/W2766437506","https://openalex.org/W2783929588","https://openalex.org/W2789442959","https://openalex.org/W2794361994","https://openalex.org/W2808983332","https://openalex.org/W2944948027","https://openalex.org/W2951506174","https://openalex.org/W2952100718","https://openalex.org/W2983248500","https://openalex.org/W2984472267","https://openalex.org/W2994080338","https://openalex.org/W3005104413","https://openalex.org/W3005483243","https://openalex.org/W3013135919","https://openalex.org/W3014985924","https://openalex.org/W3020061516","https://openalex.org/W3039795571","https://openalex.org/W3096010551","https://openalex.org/W3108065600","https://openalex.org/W3111092509","https://openalex.org/W3122872001","https://openalex.org/W3124271842","https://openalex.org/W3128821036","https://openalex.org/W3135017744","https://openalex.org/W3165135330","https://openalex.org/W3172180133","https://openalex.org/W3193599673","https://openalex.org/W3197899290","https://openalex.org/W4200228129","https://openalex.org/W4214673148","https://openalex.org/W4220867642","https://openalex.org/W4224937177","https://openalex.org/W4307274169","https://openalex.org/W4308523008","https://openalex.org/W4311820912","https://openalex.org/W4320730824","https://openalex.org/W4367601845","https://openalex.org/W4378212544","https://openalex.org/W4378903215","https://openalex.org/W4386923465","https://openalex.org/W4390546694","https://openalex.org/W4409167519"],"related_works":[],"abstract_inverted_index":{"Single-cell":[0],"RNA":[1,29],"sequencing":[2],"(scRNA-Seq)":[3],"provides":[4],"valuable":[5],"insights":[6],"into":[7],"cell":[8,41,57,81,136,173],"biology.":[9],"However,":[10],"current":[11,89,190],"scRNA-Seq":[12,116],"analytic":[13],"approaches":[14],"do":[15],"not":[16,85],"distinguish":[17],"between":[18,143],"spliced":[19,64,155],"and":[20,100,109,146,151,161,169,194],"unspliced":[21,37,70],"mRNA":[22,38,65,71],"at":[23],"the":[24,34,88,107,144],"level":[25],"of":[26,36,46,76,91,115,154],"dimensionality":[27],"reduction.":[28],"velocity":[30],"paradigm":[31],"suggests":[32],"that":[33],"presence":[35],"reflects":[39],"transitional":[40],"states,":[42],"informative":[43],"for":[44,73,198],"studies":[45],"dynamic":[47],"processes":[48],"such":[49],"as":[50],"embryogenesis":[51],"or":[52],"tissue":[53],"regeneration.":[54],"Alternatively,":[55],"stable":[56],"subsets":[58,82],"may":[59,83],"also":[60,96,176],"maintain":[61],"translationally":[62],"repressed":[63],"(e.g.,":[66],"in":[67,87,97,101,106,164,189],"P-bodies)":[68],"and/or":[69],"reservoirs":[72],"prompt":[74],"initiation":[75],"transcription-independent":[77],"expression.":[78],"Thus,":[79],"functional":[80],"differ":[84],"only":[86],"levels":[90,153],"actively":[92],"produced":[93],"mRNAs,":[94],"but":[95],"which":[98],"mRNAs":[99,163],"what":[102],"forms":[103],"are":[104],"stored":[105],"nucleus":[108],"cytoplasm.":[110],"To":[111],"enable":[112],"splicing-aware":[113],"analysis":[114,192],"data,":[117],"we":[118],"developed":[119],"a":[120,140],"method":[121],"called":[122],"SANSARA":[123,129,175,184],"(Splicing-Aware":[124],"scrNa-Seq":[125],"AppRoAch).":[126],"We":[127],"employed":[128],"to":[130,187],"characterize":[131],"peripheral":[132],"blood":[133],"regulatory":[134],"T":[135,172],"(Treg)":[137],"subsets,":[138,174],"revealing":[139],"complementary":[141],"interplay":[142],"FOXP3":[145],"Helios":[147],"master":[148],"transcription":[149],"factors":[150],"high":[152],"IL10RA,":[156],"LGALS3,":[157],"FCRL3,":[158],"CD38,":[159],"ITGAL,":[160],"LEF1":[162],"effector":[165],"Tregs.":[166],"Among":[167],"Th1":[168],"cytotoxic":[170],"CD4+":[171],"revealed":[177],"substantial":[178],"splicing":[179],"heterogeneity":[180],"across":[181],"subset-specific":[182],"genes.":[183],"is":[185],"straightforward":[186],"implement":[188],"data":[191],"pipelines":[193],"opens":[195],"new":[196],"dimensions":[197],"scRNA-Seq-based":[199],"discoveries.":[200]},"counts_by_year":[],"updated_date":"2026-04-09T08:11:56.329763","created_date":"2025-11-10T00:00:00"}