{"id":"https://openalex.org/W2590809986","doi":"https://doi.org/10.1186/s12918-017-0408-2","title":"Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris","display_name":"Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris","publication_year":2017,"publication_date":"2017-02-21","ids":{"openalex":"https://openalex.org/W2590809986","doi":"https://doi.org/10.1186/s12918-017-0408-2","mag":"2590809986","pmid":"https://pubmed.ncbi.nlm.nih.gov/28222737"},"language":"en","primary_location":{"id":"doi:10.1186/s12918-017-0408-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12918-017-0408-2","pdf_url":"https://bmcsystbiol.biomedcentral.com/track/pdf/10.1186/s12918-017-0408-2","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://bmcsystbiol.biomedcentral.com/track/pdf/10.1186/s12918-017-0408-2","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007108501","display_name":"Francisco Saitua","orcid":null},"institutions":[{"id":"https://openalex.org/I162148367","display_name":"Pontificia Universidad Cat\u00f3lica de Chile","ror":"https://ror.org/04teye511","country_code":"CL","type":"education","lineage":["https://openalex.org/I162148367"]}],"countries":["CL"],"is_corresponding":true,"raw_author_name":"Francisco Saitua","raw_affiliation_strings":["Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile","institution_ids":["https://openalex.org/I162148367"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079897099","display_name":"Paulina Torres","orcid":"https://orcid.org/0000-0001-5569-9830"},"institutions":[{"id":"https://openalex.org/I162148367","display_name":"Pontificia Universidad Cat\u00f3lica de Chile","ror":"https://ror.org/04teye511","country_code":"CL","type":"education","lineage":["https://openalex.org/I162148367"]}],"countries":["CL"],"is_corresponding":false,"raw_author_name":"Paulina Torres","raw_affiliation_strings":["Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile","institution_ids":["https://openalex.org/I162148367"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015284843","display_name":"Jos\u00e9 Ricardo P\u00e9rez\u2010Correa","orcid":"https://orcid.org/0000-0002-1278-7782"},"institutions":[{"id":"https://openalex.org/I162148367","display_name":"Pontificia Universidad Cat\u00f3lica de Chile","ror":"https://ror.org/04teye511","country_code":"CL","type":"education","lineage":["https://openalex.org/I162148367"]}],"countries":["CL"],"is_corresponding":false,"raw_author_name":"Jos\u00e9 Ricardo P\u00e9rez-Correa","raw_affiliation_strings":["Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile","institution_ids":["https://openalex.org/I162148367"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007554807","display_name":"Eduardo Agos\u00edn","orcid":"https://orcid.org/0000-0003-1656-150X"},"institutions":[{"id":"https://openalex.org/I162148367","display_name":"Pontificia Universidad Cat\u00f3lica de Chile","ror":"https://ror.org/04teye511","country_code":"CL","type":"education","lineage":["https://openalex.org/I162148367"]}],"countries":["CL"],"is_corresponding":false,"raw_author_name":"Eduardo Agosin","raw_affiliation_strings":["Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile. agosin@ing.puc.cl","Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile. agosin@ing.puc.cl","institution_ids":[]},{"raw_affiliation_string":"Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Cat\u00f3lica de Chile, Avenida Vicu\u00f1a Mackenna 4860, Santiago, Chile","institution_ids":["https://openalex.org/I162148367"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5007108501"],"corresponding_institution_ids":["https://openalex.org/I162148367"],"apc_list":null,"apc_paid":null,"fwci":2.9114,"has_fulltext":true,"cited_by_count":60,"citation_normalized_percentile":{"value":0.91566142,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"11","issue":"1","first_page":"27","last_page":"27"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8248000144958496,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.8248000144958496,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.08799999952316284,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.06970000267028809,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pichia-pastoris","display_name":"Pichia pastoris","score":0.8993514776229858},{"id":"https://openalex.org/keywords/flux-balance-analysis","display_name":"Flux balance analysis","score":0.6999819278717041},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5712562799453735},{"id":"https://openalex.org/keywords/yeast","display_name":"Yeast","score":0.5447347164154053},{"id":"https://openalex.org/keywords/identifiability","display_name":"Identifiability","score":0.5369309186935425},{"id":"https://openalex.org/keywords/systems-biology","display_name":"Systems biology","score":0.5130264163017273},{"id":"https://openalex.org/keywords/biochemical-engineering","display_name":"Biochemical engineering","score":0.5055222511291504},{"id":"https://openalex.org/keywords/chemostat","display_name":"Chemostat","score":0.49048730731010437},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.4767928123474121},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.47176221013069153},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"Metabolic engineering","score":0.4586105942726135},{"id":"https://openalex.org/keywords/synthetic-biology","display_name":"Synthetic biology","score":0.45679783821105957},{"id":"https://openalex.org/keywords/pichia","display_name":"Pichia","score":0.4449545443058014},{"id":"https://openalex.org/keywords/robustness","display_name":"Robustness (evolution)","score":0.43953871726989746},{"id":"https://openalex.org/keywords/biotechnology","display_name":"Biotechnology","score":0.38348302245140076},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.37355732917785645},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.2095058560371399},{"id":"https://openalex.org/keywords/recombinant-dna","display_name":"Recombinant DNA","score":0.16231101751327515},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.13731643557548523},{"id":"https://openalex.org/keywords/machine-learning","display_name":"Machine learning","score":0.12464529275894165}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.8993514776229858},{"id":"https://openalex.org/C167091322","wikidata":"https://www.wikidata.org/wiki/Q5463029","display_name":"Flux balance analysis","level":2,"score":0.6999819278717041},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5712562799453735},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5447347164154053},{"id":"https://openalex.org/C122770356","wikidata":"https://www.wikidata.org/wiki/Q1656753","display_name":"Identifiability","level":2,"score":0.5369309186935425},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.5130264163017273},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.5055222511291504},{"id":"https://openalex.org/C125111812","wikidata":"https://www.wikidata.org/wiki/Q902311","display_name":"Chemostat","level":3,"score":0.49048730731010437},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.4767928123474121},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47176221013069153},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.4586105942726135},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.45679783821105957},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.4449545443058014},{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.43953871726989746},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.38348302245140076},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.37355732917785645},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2095058560371399},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.16231101751327515},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13731643557548523},{"id":"https://openalex.org/C119857082","wikidata":"https://www.wikidata.org/wiki/Q2539","display_name":"Machine learning","level":1,"score":0.12464529275894165},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000332","descriptor_name":"Aerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000332","descriptor_name":"Aerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000332","descriptor_name":"Aerobiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005110","descriptor_name":"Extracellular Space","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012709","descriptor_name":"Serum Albumin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012709","descriptor_name":"Serum Albumin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012709","descriptor_name":"Serum Albumin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016681","descriptor_name":"Genome, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016681","descriptor_name":"Genome, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016681","descriptor_name":"Genome, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1186/s12918-017-0408-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12918-017-0408-2","pdf_url":"https://bmcsystbiol.biomedcentral.com/track/pdf/10.1186/s12918-017-0408-2","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},{"id":"pmid:28222737","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28222737","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC systems biology","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:5320773","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5320773","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Syst Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/s12918-017-0408-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12918-017-0408-2","pdf_url":"https://bmcsystbiol.biomedcentral.com/track/pdf/10.1186/s12918-017-0408-2","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G11155968","display_name":null,"funder_award_id":"11CEII-9568","funder_id":"https://openalex.org/F4320311278","funder_display_name":"Corporaci\u00f3n de Fomento de la Producci\u00f3n"},{"id":"https://openalex.org/G2058874265","display_name":null,"funder_award_id":"Project 11CEII-9568","funder_id":"https://openalex.org/F4320311278","funder_display_name":"Corporaci\u00f3n de Fomento de la Producci\u00f3n"},{"id":"https://openalex.org/G3233254068","display_name":null,"funder_award_id":"22140230","funder_id":"https://openalex.org/F4320334812","funder_display_name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"},{"id":"https://openalex.org/G48136981","display_name":null,"funder_award_id":"21140759","funder_id":"https://openalex.org/F4320334812","funder_display_name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"}],"funders":[{"id":"https://openalex.org/F4320311278","display_name":"Corporaci\u00f3n de Fomento de la Producci\u00f3n","ror":"https://ror.org/00as45r68"},{"id":"https://openalex.org/F4320334812","display_name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","ror":"https://ror.org/02ap3w078"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2590809986.pdf","grobid_xml":"https://content.openalex.org/works/W2590809986.grobid-xml"},"referenced_works_count":88,"referenced_works":["https://openalex.org/W152301725","https://openalex.org/W829478918","https://openalex.org/W1158629448","https://openalex.org/W1489720547","https://openalex.org/W1574470162","https://openalex.org/W1588504519","https://openalex.org/W1597483105","https://openalex.org/W1604790719","https://openalex.org/W1964812124","https://openalex.org/W1967091544","https://openalex.org/W1978486653","https://openalex.org/W1990577248","https://openalex.org/W2003035995","https://openalex.org/W2006615732","https://openalex.org/W2008141632","https://openalex.org/W2010155325","https://openalex.org/W2010535228","https://openalex.org/W2011778636","https://openalex.org/W2016229032","https://openalex.org/W2018372171","https://openalex.org/W2019133950","https://openalex.org/W2021909746","https://openalex.org/W2032013069","https://openalex.org/W2032612143","https://openalex.org/W2046594775","https://openalex.org/W2048596643","https://openalex.org/W2051403769","https://openalex.org/W2053118024","https://openalex.org/W2054506883","https://openalex.org/W2055792787","https://openalex.org/W2056756011","https://openalex.org/W2059639708","https://openalex.org/W2065665575","https://openalex.org/W2068945449","https://openalex.org/W2069353545","https://openalex.org/W2069940872","https://openalex.org/W2072558684","https://openalex.org/W2073024849","https://openalex.org/W2073259326","https://openalex.org/W2076041897","https://openalex.org/W2083855785","https://openalex.org/W2085989413","https://openalex.org/W2085993965","https://openalex.org/W2086988962","https://openalex.org/W2087737484","https://openalex.org/W2088245061","https://openalex.org/W2088691956","https://openalex.org/W2089449076","https://openalex.org/W2091515710","https://openalex.org/W2092358230","https://openalex.org/W2095691234","https://openalex.org/W2101127697","https://openalex.org/W2102164505","https://openalex.org/W2104510025","https://openalex.org/W2110585362","https://openalex.org/W2111421616","https://openalex.org/W2112736223","https://openalex.org/W2114071827","https://openalex.org/W2120357935","https://openalex.org/W2129217817","https://openalex.org/W2129877320","https://openalex.org/W2134221731","https://openalex.org/W2135624707","https://openalex.org/W2135718750","https://openalex.org/W2135811479","https://openalex.org/W2137128504","https://openalex.org/W2143696976","https://openalex.org/W2143861551","https://openalex.org/W2145658930","https://openalex.org/W2145885789","https://openalex.org/W2147472054","https://openalex.org/W2147901684","https://openalex.org/W2150569643","https://openalex.org/W2151239298","https://openalex.org/W2154018668","https://openalex.org/W2155333588","https://openalex.org/W2155638586","https://openalex.org/W2164293128","https://openalex.org/W2170160990","https://openalex.org/W2267352312","https://openalex.org/W2312891283","https://openalex.org/W2314807489","https://openalex.org/W2326035029","https://openalex.org/W2379350595","https://openalex.org/W2917973694","https://openalex.org/W4235508806","https://openalex.org/W4238101851","https://openalex.org/W4300713618"],"related_works":["https://openalex.org/W1975687798","https://openalex.org/W2256808269","https://openalex.org/W2393867567","https://openalex.org/W1480951157","https://openalex.org/W2394330041","https://openalex.org/W2368039950","https://openalex.org/W2414398983","https://openalex.org/W2369041049","https://openalex.org/W2363862718","https://openalex.org/W2032991144"],"abstract_inverted_index":{"BACKGROUND:":[0],"Pichia":[1,98,311],"pastoris":[2,69,312,345],"shows":[3],"physiological":[4],"advantages":[5],"in":[6,22,79,171,187,287],"producing":[7],"recombinant":[8,223],"proteins,":[9],"compared":[10],"to":[11,74,116,200,211,218,330,338],"other":[12],"commonly":[13],"used":[14,73],"cell":[15,49,77],"factories.":[16],"This":[17],"yeast":[18],"is":[19,30,57],"mostly":[20],"grown":[21],"dynamic":[23,89,305,320],"cultivation":[24],"systems,":[25],"where":[26,163],"the":[27,52,128,148,158,175,180,188,192,202,220,253,267,279,292,296,340],"cell's":[28],"environment":[29],"continuously":[31],"changing":[32],"and":[33,47,108,124,184,210,214,239,264,334],"many":[34],"variables":[35],"influence":[36],"process":[37,215,335],"productivity.":[38],"In":[39,83,299],"this":[40,84,300],"context,":[41],"a":[42,88,135,197,207,273,283,288,304,343],"model":[43,92,104,119,159,176,193,268,309,323],"capable":[44],"of":[45,55,67,97,145,150,182,206,222,229,235,247,272,282,291,295,310,342,347],"explaining":[46],"predicting":[48],"behavior":[50,78],"for":[51,93,106,142,179],"rational":[53],"design":[54],"bioprocesses":[56],"highly":[58],"desirable.":[59],"Currently,":[60],"there":[61],"are":[62],"five":[63],"genome-scale":[64,90],"metabolic":[65,91,203,308,316],"reconstructions":[66],"P.":[68,344],"which":[70],"have":[71],"been":[72],"predict":[75],"extracellular":[76],"stationary":[80],"conditions.":[81,190],"RESULTS:":[82],"work,":[85,301],"we":[86,111,302],"assembled":[87],"glucose-limited,":[94],"aerobic":[95],"cultivations":[96],"pastoris.":[99],"Starting":[100],"from":[101],"an":[102],"initial":[103],"structure":[105],"batch":[107,183],"fed-batch":[109,185,208,284],"cultures,":[110],"performed":[112],"pre/post":[113],"regression":[114],"diagnostics":[115],"ensure":[117],"that":[118,233,270,313],"parameters":[120],"were":[121,131,140],"identifiable,":[122],"significant":[123],"sensitive.":[125],"Once":[126],"identified,":[127],"non-relevant":[129],"ones":[130],"iteratively":[132],"fixed":[133],"until":[134],"priori":[136],"robust":[137],"modeling":[138],"structures":[139,153],"found":[141],"each":[143],"type":[144],"cultivation.":[146],"Next,":[147],"robustness":[149],"these":[151],"reduced":[152],"was":[154,177,194],"confirmed":[155],"by":[156,257],"calibrating":[157],"with":[160,327],"new":[161],"datasets,":[162],"no":[164],"sensitivity,":[165],"identifiability":[166],"or":[167],"significance":[168],"problems":[169],"appeared":[170],"their":[172],"parameters.":[173],"Afterwards,":[174],"validated":[178],"prediction":[181],"dynamics":[186],"studied":[189],"Lastly,":[191],"employed":[195],"as":[196],"case":[198],"study":[199],"analyze":[201],"flux":[204,317],"distribution":[205],"culture":[209,285],"unravel":[212],"genetic":[213,333],"engineering":[216,336],"strategies":[217,337],"improve":[219,339],"production":[221],"Human":[224],"Serum":[225],"Albumin":[226],"(HSA).":[227],"Simulation":[228],"single":[230],"knock-outs":[231],"indicated":[232],"deviation":[234],"carbon":[236],"towards":[237],"cysteine":[238],"tryptophan":[240],"formation":[241],"improves":[242],"HSA":[243,254],"production.":[244],"The":[245,322],"deletion":[246],"methylene":[248],"tetrahydrofolate":[249],"dehydrogenase":[250],"could":[251],"increase":[252,290],"volumetric":[255,293],"productivity":[256,294],"630%.":[258],"Moreover,":[259],"given":[260],"specific":[261,275],"bioprocess":[262],"limitations":[263],"strain":[265,346],"characteristics,":[266],"suggests":[269],"implementation":[271],"decreasing":[274],"growth":[276],"rate":[277],"during":[278],"feed":[280],"phase":[281],"results":[286],"25%":[289],"protein.":[297],"CONCLUSION:":[298],"formulated":[303],"genome":[306],"scale":[307],"yields":[314],"realistic":[315],"distributions":[318],"throughout":[319],"cultivations.":[321],"can":[324],"be":[325],"calibrated":[326],"experimental":[328],"data":[329],"rationally":[331],"propose":[332],"performance":[341],"interest.":[348]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":5}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}