{"id":"https://openalex.org/W4396934436","doi":"https://doi.org/10.1186/s12859-024-05809-3","title":"Readsynth: short-read simulation for consideration of composition-biases in reduced metagenome sequencing approaches","display_name":"Readsynth: short-read simulation for consideration of composition-biases in reduced metagenome sequencing approaches","publication_year":2024,"publication_date":"2024-05-15","ids":{"openalex":"https://openalex.org/W4396934436","doi":"https://doi.org/10.1186/s12859-024-05809-3","pmid":"https://pubmed.ncbi.nlm.nih.gov/38750423"},"language":"en","primary_location":{"id":"doi:10.1186/s12859-024-05809-3","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1186/s12859-024-05809-3","pdf_url":"https://link.springer.com/content/pdf/10.1186/s12859-024-05809-3.pdf","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://link.springer.com/content/pdf/10.1186/s12859-024-05809-3.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046453564","display_name":"Ryan Kuster","orcid":"https://orcid.org/0000-0003-2477-090X"},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ryan Kuster","raw_affiliation_strings":["Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA. rkuster@utk.edu","Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA. rkuster@utk.edu","institution_ids":["https://openalex.org/I75027704"]},{"raw_affiliation_string":"Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA","institution_ids":["https://openalex.org/I75027704"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021572745","display_name":"Margaret Staton","orcid":"https://orcid.org/0000-0003-2971-9353"},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Margaret Staton","raw_affiliation_strings":["Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA","institution_ids":["https://openalex.org/I75027704"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5046453564"],"corresponding_institution_ids":["https://openalex.org/I75027704"],"apc_list":{"value":2790,"currency":"USD","value_usd":2790},"apc_paid":{"value":2790,"currency":"USD","value_usd":2790},"fwci":0.1643,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.47091572,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":97},"biblio":{"volume":"25","issue":"1","first_page":"191","last_page":"191"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.8330000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.12759999930858612,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.008799999952316284,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/metagenomics","display_name":"Metagenomics","score":0.9001993536949158},{"id":"https://openalex.org/keywords/software","display_name":"Software","score":0.5397002100944519},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5221631526947021},{"id":"https://openalex.org/keywords/deep-sequencing","display_name":"Deep sequencing","score":0.507845938205719},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.48358768224716187},{"id":"https://openalex.org/keywords/profiling","display_name":"Profiling (computer programming)","score":0.47661033272743225},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.44997698068618774},{"id":"https://openalex.org/keywords/amplicon","display_name":"Amplicon","score":0.4458268880844116},{"id":"https://openalex.org/keywords/data-mining","display_name":"Data mining","score":0.4333884119987488},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.42789649963378906},{"id":"https://openalex.org/keywords/amplicon-sequencing","display_name":"Amplicon sequencing","score":0.42333418130874634},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.3146476745605469},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.1974354088306427},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.11307734251022339}],"concepts":[{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.9001993536949158},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.5397002100944519},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5221631526947021},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.507845938205719},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.48358768224716187},{"id":"https://openalex.org/C187191949","wikidata":"https://www.wikidata.org/wiki/Q1138496","display_name":"Profiling (computer programming)","level":2,"score":0.47661033272743225},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.44997698068618774},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.4458268880844116},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.4333884119987488},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42789649963378906},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.42333418130874634},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.3146476745605469},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1974354088306427},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11307734251022339},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000069196","descriptor_name":"Gastrointestinal Microbiome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000069196","descriptor_name":"Gastrointestinal Microbiome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000069196","descriptor_name":"Gastrointestinal Microbiome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000069196","descriptor_name":"Gastrointestinal Microbiome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D054892","descriptor_name":"Metagenome","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1186/s12859-024-05809-3","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1186/s12859-024-05809-3","pdf_url":"https://link.springer.com/content/pdf/10.1186/s12859-024-05809-3.pdf","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmid:38750423","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38750423","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:11095026","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11095026","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11095026/pdf/12859_2024_Article_5809.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:2eba3d3d84d54d2493f7dae9cfb4fcc6","is_oa":true,"landing_page_url":"https://doaj.org/article/2eba3d3d84d54d2493f7dae9cfb4fcc6","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, Vol 25, Iss 1, Pp 1-18 (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/s12859-024-05809-3","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1186/s12859-024-05809-3","pdf_url":"https://link.springer.com/content/pdf/10.1186/s12859-024-05809-3.pdf","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4396934436.pdf","grobid_xml":"https://content.openalex.org/works/W4396934436.grobid-xml"},"referenced_works_count":25,"referenced_works":["https://openalex.org/W2036897871","https://openalex.org/W2085222782","https://openalex.org/W2095498620","https://openalex.org/W2095882149","https://openalex.org/W2102308328","https://openalex.org/W2103441770","https://openalex.org/W2135293770","https://openalex.org/W2142802314","https://openalex.org/W2159474015","https://openalex.org/W2161184021","https://openalex.org/W2169773990","https://openalex.org/W2263977295","https://openalex.org/W2345658906","https://openalex.org/W2418933939","https://openalex.org/W2792871760","https://openalex.org/W2886557383","https://openalex.org/W2952074209","https://openalex.org/W2990618091","https://openalex.org/W3014821714","https://openalex.org/W3079060667","https://openalex.org/W3113153648","https://openalex.org/W3131055609","https://openalex.org/W4210472216","https://openalex.org/W4247053599","https://openalex.org/W4386372879"],"related_works":["https://openalex.org/W2506843120","https://openalex.org/W2945285105","https://openalex.org/W4390154386","https://openalex.org/W2306179760","https://openalex.org/W4206175384","https://openalex.org/W2940838442","https://openalex.org/W2979695756","https://openalex.org/W2051814207","https://openalex.org/W2093083173","https://openalex.org/W4360610477"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,194,215],"application":[2,97],"of":[3,40,49,60,76,98,116,146,158,168,181,196,225,241],"reduced":[4,231],"metagenomic":[5,54,105,227],"sequencing":[6,17,21,55,106,121,233],"approaches":[7,22],"holds":[8],"promise":[9],"as":[10,29,86,88],"a":[11,30,147,230],"middle":[12],"ground":[13],"between":[14],"targeted":[15],"amplicon":[16],"and":[18,71,96,120,138,179,199,213,245],"whole":[19],"metagenome":[20],"but":[23,235],"has":[24],"not":[25],"been":[26],"widely":[27],"adopted":[28],"technique.":[31],"A":[32],"major":[33],"barrier":[34],"to":[35,45,67,83,112,142,166,186],"adoption":[36],"is":[37],"the":[38,72,94,114,176,183,222,239,247],"lack":[39],"read":[41,129],"simulation":[42,99],"software":[43],"built":[44],"handle":[46],"characteristic":[47],"features":[48],"these":[50,77,143],"novel":[51,81,148],"approaches.":[52],"Reduced":[53],"(RMS)":[56],"produces":[57],"unique":[58],"patterns":[59],"fragmentation":[61],"per":[62,131],"genome":[63],"that":[64,209],"are":[65,206],"sensitive":[66],"restriction":[68,197],"enzyme":[69],"choice,":[70],"non-uniform":[73],"size":[74,200],"selection":[75,201],"fragments":[78],"may":[79],"introduce":[80],"challenges":[82],"taxonomic":[84,153],"assignment":[85],"well":[87],"relative":[89],"abundance":[90],"estimates.":[91],"RESULTS:":[92],"Through":[93],"development":[95,240],"software,":[100],"readsynth,":[101],"we":[102],"compare":[103],"simulated":[104,159],"libraries":[107,228],"with":[108,192,229],"existing":[109],"RMS":[110,184],"data":[111,249],"assess":[113],"influence":[115],"multiple":[117],"library":[118,204],"preparation":[119],"steps":[122,202],"on":[123,128],"downstream":[124,211],"analytical":[125],"results.":[126],"Based":[127],"depth":[130],"position,":[132],"readsynth":[133],"achieved":[134],"0.79":[135],"Pearson's":[136],"correlation":[137,141],"0.94":[139],"Spearman's":[140],"benchmarks.":[144],"Application":[145],"estimation":[149],"approach,":[150,234],"fixed":[151],"length":[152],"ratios,":[154],"improved":[155],"quantification":[156],"accuracy":[157],"human":[160],"gut":[161],"microbial":[162,188],"communities":[163,189],"when":[164],"compared":[165],"estimates":[167],"mean":[169],"or":[170],"median":[171],"coverage.":[172],"CONCLUSIONS:":[173],"We":[174],"investigate":[175],"possible":[177,223],"strengths":[178],"weaknesses":[180],"applying":[182],"technique":[185],"profiling":[187,212],"via":[190],"simulations":[191,216],"readsynth.":[193],"choice":[195],"enzymes":[198],"in":[203,218],"prep":[205],"non-trivial":[207],"decisions":[208],"bias":[210],"quantification.":[214],"investigated":[217],"this":[219,252],"study":[220],"illustrate":[221],"limits":[224],"preparing":[226],"representation":[232],"also":[236],"allow":[237],"for":[238,243],"strategies":[242],"producing":[244],"handling":[246],"sequence":[248],"produced":[250],"by":[251],"promising":[253],"application.":[254]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-07-23T08:03:31.855105","created_date":"2025-10-10T00:00:00"}
