{"id":"https://openalex.org/W3135625811","doi":"https://doi.org/10.1186/s12859-021-04034-6","title":"PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants","display_name":"PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants","publication_year":2021,"publication_date":"2021-03-02","ids":{"openalex":"https://openalex.org/W3135625811","doi":"https://doi.org/10.1186/s12859-021-04034-6","mag":"3135625811","pmid":"https://pubmed.ncbi.nlm.nih.gov/33653259"},"language":"en","primary_location":{"id":"doi:10.1186/s12859-021-04034-6","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12859-021-04034-6","pdf_url":"https://bmcbioinformatics.biomedcentral.com/track/pdf/10.1186/s12859-021-04034-6","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbioinformatics.biomedcentral.com/track/pdf/10.1186/s12859-021-04034-6","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057146838","display_name":"Sebastian M. Siegner","orcid":"https://orcid.org/0000-0001-6811-0474"},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Sebastian M. Siegner","raw_affiliation_strings":["Department of Biology, ETH Zurich, Zurich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011906904","display_name":"Mehmet E. Karasu","orcid":"https://orcid.org/0000-0002-1918-9152"},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Mehmet E. Karasu","raw_affiliation_strings":["Department of Biology, ETH Zurich, Zurich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101625610","display_name":"Markus Schr\u00f6der","orcid":"https://orcid.org/0000-0001-5942-1254"},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Markus S. Schr\u00f6der","raw_affiliation_strings":["Department of Biology, ETH Zurich, Zurich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027497357","display_name":"Zacharias Kontarakis","orcid":"https://orcid.org/0000-0001-8825-8513"},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Zacharias Kontarakis","raw_affiliation_strings":["Department of Biology, ETH Zurich, Zurich, Switzerland","Genome Engineering and Measurement Lab, ETH Zurich, Zurich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]},{"raw_affiliation_string":"Genome Engineering and Measurement Lab, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073502390","display_name":"Jacob E. Corn","orcid":"https://orcid.org/0000-0002-7798-5309"},"institutions":[{"id":"https://openalex.org/I35440088","display_name":"ETH Zurich","ror":"https://ror.org/05a28rw58","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I35440088"]}],"countries":["CH"],"is_corresponding":true,"raw_author_name":"Jacob E. Corn","raw_affiliation_strings":["Department of Biology, ETH Zurich, Zurich, Switzerland. jacob.corn@biol.ethz.ch","Department of Biology, ETH Zurich, Zurich, Switzerland"],"raw_orcid":"https://orcid.org/0000-0002-7798-5309","affiliations":[{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland. jacob.corn@biol.ethz.ch","institution_ids":["https://openalex.org/I35440088"]},{"raw_affiliation_string":"Department of Biology, ETH Zurich, Zurich, Switzerland","institution_ids":["https://openalex.org/I35440088"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5073502390"],"corresponding_institution_ids":["https://openalex.org/I35440088"],"apc_list":{"value":1690,"currency":"GBP","value_usd":2072},"apc_paid":{"value":1432,"currency":"EUR","value_usd":1544},"fwci":26.8829,"has_fulltext":true,"cited_by_count":437,"citation_normalized_percentile":{"value":0.99846992,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":100},"biblio":{"volume":"22","issue":"1","first_page":"101","last_page":"101"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.004999999888241291,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.835712194442749},{"id":"https://openalex.org/keywords/cas9","display_name":"Cas9","score":0.6285069584846497},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.607528567314148},{"id":"https://openalex.org/keywords/genome-editing","display_name":"Genome editing","score":0.6050048470497131},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5403793454170227},{"id":"https://openalex.org/keywords/toolbox","display_name":"Toolbox","score":0.5023989677429199},{"id":"https://openalex.org/keywords/guide-rna","display_name":"Guide RNA","score":0.46488142013549805},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.4134364426136017},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.25358256697654724},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.2189716100692749},{"id":"https://openalex.org/keywords/programming-language","display_name":"Programming language","score":0.15118694305419922}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.835712194442749},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.6285069584846497},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.607528567314148},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6050048470497131},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5403793454170227},{"id":"https://openalex.org/C2777655017","wikidata":"https://www.wikidata.org/wiki/Q1501161","display_name":"Toolbox","level":2,"score":0.5023989677429199},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.46488142013549805},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4134364426136017},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.25358256697654724},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2189716100692749},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.15118694305419922}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003596","descriptor_name":"Cytosine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017394","descriptor_name":"RNA, Guide, Kinetoplastida","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":8,"locations":[{"id":"doi:10.1186/s12859-021-04034-6","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12859-021-04034-6","pdf_url":"https://bmcbioinformatics.biomedcentral.com/track/pdf/10.1186/s12859-021-04034-6","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmid:33653259","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33653259","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":null},{"id":"pmh:oai:www.zora.uzh.ch:210782","is_oa":true,"landing_page_url":null,"pdf_url":null,"source":{"id":"https://openalex.org/S4306401281","display_name":"Zurich Open Repository and Archive (University of Zurich)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I202697423","host_organization_name":"University of Zurich","host_organization_lineage":["https://openalex.org/I202697423"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Siegner, Sebastian M; Karasu, Mehmet E; Schr\u00f6der, Markus S; Kontarakis, Zacharias; Corn, Jacob E  (2021). PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants.  BMC Bioinformatics, 22:101.","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:0a978ceebc854d2f843921067c29d8f5","is_oa":true,"landing_page_url":"https://doaj.org/article/0a978ceebc854d2f843921067c29d8f5","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, Vol 22, Iss 1, Pp 1-12 (2021)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7923538","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7923538","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"},{"id":"pmh:oai:www.research-collection.ethz.ch:20.500.11850/474339","is_oa":true,"landing_page_url":"http://hdl.handle.net/20.500.11850/474339","pdf_url":null,"source":{"id":"https://openalex.org/S4306402302","display_name":"Repository for Publications and Research Data (ETH Zurich)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I35440088","host_organization_name":"ETH Zurich","host_organization_lineage":["https://openalex.org/I35440088"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, 22 (1)","raw_type":"info:eu-repo/semantics/article"},{"id":"doi:10.3929/ethz-b-000474339","is_oa":true,"landing_page_url":"https://doi.org/10.3929/ethz-b-000474339","pdf_url":null,"source":{"id":"https://openalex.org/S7407051236","display_name":"ETH Z\u00fcrich Research Collection","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.5167/uzh-210782","is_oa":true,"landing_page_url":"https://doi.org/10.5167/uzh-210782","pdf_url":null,"source":{"id":"https://openalex.org/S7407051291","display_name":"Universit\u00e4t Z\u00fcrich, ZORA","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.1186/s12859-021-04034-6","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12859-021-04034-6","pdf_url":"https://bmcbioinformatics.biomedcentral.com/track/pdf/10.1186/s12859-021-04034-6","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306947","display_name":"Fanconi Anemia Research Fund","ror":"https://ror.org/04w07g537"},{"id":"https://openalex.org/F4320325162","display_name":"NOMIS Stiftung","ror":"https://ror.org/01zkd3118"},{"id":"https://openalex.org/F4320336859","display_name":"Functional Genomics Center Zurich","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3135625811.pdf","grobid_xml":"https://content.openalex.org/works/W3135625811.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1608084731","https://openalex.org/W1922040927","https://openalex.org/W1973094248","https://openalex.org/W2115603949","https://openalex.org/W2154866190","https://openalex.org/W2160697532","https://openalex.org/W2336828812","https://openalex.org/W2594683972","https://openalex.org/W2737091810","https://openalex.org/W2766599608","https://openalex.org/W2769863451","https://openalex.org/W2803581084","https://openalex.org/W2805505209","https://openalex.org/W2806478486","https://openalex.org/W2883250809","https://openalex.org/W2888908334","https://openalex.org/W2889513238","https://openalex.org/W2894894185","https://openalex.org/W2897295193","https://openalex.org/W2915177587","https://openalex.org/W2916364858","https://openalex.org/W2939749480","https://openalex.org/W2946692293","https://openalex.org/W2950114027","https://openalex.org/W2951233129","https://openalex.org/W2971914211","https://openalex.org/W2981137429","https://openalex.org/W2989635394","https://openalex.org/W2995849392","https://openalex.org/W3012016445","https://openalex.org/W3012327875","https://openalex.org/W3013504322","https://openalex.org/W3013993115","https://openalex.org/W3014506265","https://openalex.org/W3047219457","https://openalex.org/W3087701445","https://openalex.org/W4245053291","https://openalex.org/W4249468678"],"related_works":["https://openalex.org/W2603161682","https://openalex.org/W2960163646","https://openalex.org/W2807464733","https://openalex.org/W2516842346","https://openalex.org/W2134268326","https://openalex.org/W2903635918","https://openalex.org/W3000623258","https://openalex.org/W2437118042","https://openalex.org/W2337751047","https://openalex.org/W2667943050"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1],"rapid":[2],"expansion":[3],"of":[4,88,105],"the":[5,44,86,103,173,186],"CRISPR":[6,34,45,209],"toolbox":[7],"through":[8],"tagging":[9],"effector":[10],"domains":[11],"to":[12,24,58,66,84,119,146,162,166,189],"either":[13],"enzymatically":[14],"inactive":[15],"Cas9":[16,19],"(dCas9)":[17],"or":[18,36,53,126,132,168],"nickase":[20],"(nCas9)":[21],"has":[22],"led":[23],"several":[25],"promising":[26],"new":[27],"gene":[28],"editing":[29,218],"strategies.":[30],"Recent":[31],"additions":[32],"include":[33],"cytosine":[35],"adenine":[37],"base":[38],"editors":[39,47],"(CBEs":[40],"and":[41,43,68,74,91,109,178,184,211,220],"ABEs)":[42],"prime":[46],"(PEs),":[48],"in":[49,72,154],"which":[50,214],"a":[51,99,130,170,204],"deaminase":[52],"reverse":[54],"transcriptase":[55],"are":[56],"fused":[57],"nCas9,":[59],"respectively.":[60],"These":[61],"tools":[62,82],"hold":[63],"great":[64],"promise":[65],"model":[67,147],"correct":[69],"disease-causing":[70],"mutations":[71,152],"animal":[73],"plant":[75],"models.":[76],"But":[77],"so":[78],"far,":[79],"no":[80],"widely-available":[81],"exist":[83],"automate":[85],"design":[87,104,120,163,206,222],"both":[89],"BE":[90,188,212],"PE":[92,210],"reagents.":[93],"RESULTS:":[94],"We":[95],"developed":[96],"PnB":[97,114,141,157,191,200],"Designer,":[98,142],"web-based":[100],"application":[101],"for":[102,107,112,124,208],"pegRNAs":[106,145],"PEs":[108],"guide":[110,122,164],"RNAs":[111,123,165],"BEs.":[113],"Designer":[115,158,192,201],"makes":[116],"it":[117],"easy":[118],"targeting":[121],"single":[125],"multiple":[127,138],"targets":[128],"on":[129],"variant":[131],"reference":[133],"genome":[134,174],"from":[135],"organisms":[136],"spanning":[137],"kingdoms.":[139],"With":[140,199],"we":[143,202],"designed":[144],"all":[148],"known":[149],"disease":[150],"causing":[151],"available":[153],"ClinVar.":[155],"Additionally,":[156],"can":[159],"be":[160],"used":[161],"install":[167],"revert":[169],"SNV,":[171],"scanning":[172],"with":[175],"one":[176],"CBE":[177],"seven":[179],"different":[180],"ABE":[181],"PAM":[182],"variants":[183],"returning":[185],"best":[187],"use.":[190],"is":[193],"publicly":[194],"accessible":[195],"at":[196],"http://fgcz-shiny.uzh.ch/PnBDesigner/":[197],"CONCLUSION:":[198],"created":[203],"user-friendly":[205],"tool":[207],"reagents,":[213],"should":[215],"simplify":[216],"choosing":[217],"strategy":[219],"avoiding":[221],"complications.":[223]},"counts_by_year":[{"year":2026,"cited_by_count":24},{"year":2025,"cited_by_count":77},{"year":2024,"cited_by_count":102},{"year":2023,"cited_by_count":108},{"year":2022,"cited_by_count":90},{"year":2021,"cited_by_count":35},{"year":2020,"cited_by_count":1}],"updated_date":"2026-06-19T15:47:20.252518","created_date":"2025-10-10T00:00:00"}