{"id":"https://openalex.org/W2080475980","doi":"https://doi.org/10.1186/1752-0509-5-34","title":"Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products","display_name":"Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products","publication_year":2011,"publication_date":"2011-02-25","ids":{"openalex":"https://openalex.org/W2080475980","doi":"https://doi.org/10.1186/1752-0509-5-34","mag":"2080475980","pmid":"https://pubmed.ncbi.nlm.nih.gov/21352531"},"language":"en","primary_location":{"id":"doi:10.1186/1752-0509-5-34","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-5-34","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-5-34","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-5-34","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003864155","display_name":"Nuno Carinhas","orcid":"https://orcid.org/0000-0002-3321-1221"},"institutions":[{"id":"https://openalex.org/I4210159117","display_name":"Instituto de Biologia Experimental e Tecnol\u00f3gica","ror":"https://ror.org/0599z7n30","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210159117"]},{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":true,"raw_author_name":"Nuno Carinhas","raw_affiliation_strings":["REQUIMTE, Systems Biology&Engineering Group, Chemistry Department, Universidade Nova de Lisboa, P-2829-516 Caparica, Portugal","Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"REQUIMTE, Systems Biology&Engineering Group, Chemistry Department, Universidade Nova de Lisboa, P-2829-516 Caparica, Portugal","institution_ids":["https://openalex.org/I83558840"]},{"raw_affiliation_string":"Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal","institution_ids":["https://openalex.org/I4210159117","https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032022895","display_name":"Vicente Bernal","orcid":"https://orcid.org/0000-0003-2482-3477"},"institutions":[{"id":"https://openalex.org/I80180929","display_name":"Universidad de Murcia","ror":"https://ror.org/03p3aeb86","country_code":"ES","type":"education","lineage":["https://openalex.org/I80180929"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Vicente Bernal","raw_affiliation_strings":["Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular B e Inmunolog\u00eda, Facultad de Qu\u00edmica, Universidad de Murcia, E-30100, Murcia, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular B e Inmunolog\u00eda, Facultad de Qu\u00edmica, Universidad de Murcia, E-30100, Murcia, Spain","institution_ids":["https://openalex.org/I80180929"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059860426","display_name":"Ana P. Teixeira","orcid":"https://orcid.org/0000-0002-9208-8737"},"institutions":[{"id":"https://openalex.org/I4210159117","display_name":"Instituto de Biologia Experimental e Tecnol\u00f3gica","ror":"https://ror.org/0599z7n30","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210159117"]},{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Ana P Teixeira","raw_affiliation_strings":["Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal","institution_ids":["https://openalex.org/I4210159117","https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021060711","display_name":"Manuel J.T. Carrondo","orcid":"https://orcid.org/0000-0002-4550-4574"},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]},{"id":"https://openalex.org/I4210138594","display_name":"Rede de Qu\u00edmica e Tecnologia","ror":"https://ror.org/04j14t006","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210138594"]},{"id":"https://openalex.org/I4210159117","display_name":"Instituto de Biologia Experimental e Tecnol\u00f3gica","ror":"https://ror.org/0599z7n30","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210159117"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Manuel JT Carrondo","raw_affiliation_strings":["Chemistry Department, REQUIMTE, Systems Biology&Engineering Group, Universidade Nova de Lisboa, P-2829-516, Caparica, Portugal","Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, REQUIMTE, Systems Biology&Engineering Group, Universidade Nova de Lisboa, P-2829-516, Caparica, Portugal","institution_ids":["https://openalex.org/I83558840","https://openalex.org/I4210138594"]},{"raw_affiliation_string":"Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal","institution_ids":["https://openalex.org/I4210159117","https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041231390","display_name":"Paula M. Alves","orcid":"https://orcid.org/0000-0003-1445-3556"},"institutions":[{"id":"https://openalex.org/I4210159117","display_name":"Instituto de Biologia Experimental e Tecnol\u00f3gica","ror":"https://ror.org/0599z7n30","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210159117"]},{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Paula M Alves","raw_affiliation_strings":["Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Tecnologia Quimica e Biol\u00f3gica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnol\u00f3gica (ITQB-UNL/IBET), Apartado 12, P-2781-901, Oeiras, Portugal","institution_ids":["https://openalex.org/I4210159117","https://openalex.org/I83558840"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034295628","display_name":"Rui Oliveira","orcid":"https://orcid.org/0000-0001-8077-4177"},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]},{"id":"https://openalex.org/I4210138594","display_name":"Rede de Qu\u00edmica e Tecnologia","ror":"https://ror.org/04j14t006","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210138594"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Rui Oliveira","raw_affiliation_strings":["Chemistry Department, REQUIMTE, Systems Biology&Engineering Group, Universidade Nova de Lisboa, P-2829-516, Caparica, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Department, REQUIMTE, Systems Biology&Engineering Group, Universidade Nova de Lisboa, P-2829-516, Caparica, Portugal","institution_ids":["https://openalex.org/I83558840","https://openalex.org/I4210138594"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5003864155"],"corresponding_institution_ids":["https://openalex.org/I4210159117","https://openalex.org/I83558840"],"apc_list":null,"apc_paid":null,"fwci":2.9266,"has_fulltext":true,"cited_by_count":52,"citation_normalized_percentile":{"value":0.91509717,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":"5","issue":"1","first_page":"34","last_page":"34"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.6665999889373779,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.6665999889373779,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.3165000081062317,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/flux-balance-analysis","display_name":"Flux balance analysis","score":0.757016658782959},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"Metabolic engineering","score":0.7265466451644897},{"id":"https://openalex.org/keywords/systems-biology","display_name":"Systems biology","score":0.6910743713378906},{"id":"https://openalex.org/keywords/metabolic-flux-analysis","display_name":"Metabolic flux analysis","score":0.6819589138031006},{"id":"https://openalex.org/keywords/context","display_name":"Context (archaeology)","score":0.6533774733543396},{"id":"https://openalex.org/keywords/in-silico","display_name":"In silico","score":0.6279019713401794},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.6119007468223572},{"id":"https://openalex.org/keywords/metabolic-network","display_name":"Metabolic network","score":0.5918626189231873},{"id":"https://openalex.org/keywords/flux","display_name":"Flux (metallurgy)","score":0.5261672735214233},{"id":"https://openalex.org/keywords/bottleneck","display_name":"Bottleneck","score":0.5166897773742676},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.4816252589225769},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.44976410269737244},{"id":"https://openalex.org/keywords/monte-carlo-method","display_name":"Monte Carlo method","score":0.418062686920166},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.4174623489379883},{"id":"https://openalex.org/keywords/identification","display_name":"Identification (biology)","score":0.4137343764305115},{"id":"https://openalex.org/keywords/biochemical-engineering","display_name":"Biochemical engineering","score":0.33559322357177734},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.14382630586624146},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.12280082702636719},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.11508351564407349},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.0968414843082428}],"concepts":[{"id":"https://openalex.org/C167091322","wikidata":"https://www.wikidata.org/wiki/Q5463029","display_name":"Flux balance analysis","level":2,"score":0.757016658782959},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.7265466451644897},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.6910743713378906},{"id":"https://openalex.org/C160941953","wikidata":"https://www.wikidata.org/wiki/Q6822342","display_name":"Metabolic flux analysis","level":3,"score":0.6819589138031006},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6533774733543396},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.6279019713401794},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6119007468223572},{"id":"https://openalex.org/C101810790","wikidata":"https://www.wikidata.org/wiki/Q2263094","display_name":"Metabolic network","level":2,"score":0.5918626189231873},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.5261672735214233},{"id":"https://openalex.org/C2780513914","wikidata":"https://www.wikidata.org/wiki/Q18210350","display_name":"Bottleneck","level":2,"score":0.5166897773742676},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.4816252589225769},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.44976410269737244},{"id":"https://openalex.org/C19499675","wikidata":"https://www.wikidata.org/wiki/Q232207","display_name":"Monte Carlo method","level":2,"score":0.418062686920166},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4174623489379883},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.4137343764305115},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.33559322357177734},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.14382630586624146},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12280082702636719},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.11508351564407349},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0968414843082428},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.0},{"id":"https://openalex.org/C149635348","wikidata":"https://www.wikidata.org/wiki/Q193040","display_name":"Embedded system","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009010","descriptor_name":"Monte Carlo Method","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009010","descriptor_name":"Monte Carlo Method","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009010","descriptor_name":"Monte Carlo Method","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018411","descriptor_name":"Spodoptera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1186/1752-0509-5-34","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-5-34","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-5-34","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},{"id":"pmid:21352531","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21352531","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC systems biology","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:3236310","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3236310","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Syst Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/1752-0509-5-34","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-5-34","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-5-34","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334779","display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","ror":"https://ror.org/00snfqn58"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2080475980.pdf","grobid_xml":"https://content.openalex.org/works/W2080475980.grobid-xml"},"referenced_works_count":57,"referenced_works":["https://openalex.org/W1006945596","https://openalex.org/W1529435619","https://openalex.org/W1574470162","https://openalex.org/W1758395617","https://openalex.org/W1969983517","https://openalex.org/W1976251851","https://openalex.org/W1981656731","https://openalex.org/W2004964623","https://openalex.org/W2005321445","https://openalex.org/W2010088414","https://openalex.org/W2014092278","https://openalex.org/W2017987256","https://openalex.org/W2026089579","https://openalex.org/W2028332226","https://openalex.org/W2030209236","https://openalex.org/W2031693543","https://openalex.org/W2035787551","https://openalex.org/W2038444936","https://openalex.org/W2042901969","https://openalex.org/W2044482436","https://openalex.org/W2052434790","https://openalex.org/W2054475777","https://openalex.org/W2055063781","https://openalex.org/W2056924389","https://openalex.org/W2057537794","https://openalex.org/W2065705538","https://openalex.org/W2071090551","https://openalex.org/W2072543338","https://openalex.org/W2073503722","https://openalex.org/W2075802407","https://openalex.org/W2077341939","https://openalex.org/W2082948758","https://openalex.org/W2084094917","https://openalex.org/W2094000957","https://openalex.org/W2094781659","https://openalex.org/W2097057782","https://openalex.org/W2098824895","https://openalex.org/W2100818174","https://openalex.org/W2101484232","https://openalex.org/W2103170157","https://openalex.org/W2103520139","https://openalex.org/W2106576546","https://openalex.org/W2109880272","https://openalex.org/W2123793943","https://openalex.org/W2126176298","https://openalex.org/W2128746754","https://openalex.org/W2130364940","https://openalex.org/W2131178894","https://openalex.org/W2133783666","https://openalex.org/W2151618686","https://openalex.org/W2154767435","https://openalex.org/W2156875887","https://openalex.org/W2160079005","https://openalex.org/W2163855763","https://openalex.org/W2164293128","https://openalex.org/W2169561592","https://openalex.org/W2610694850"],"related_works":["https://openalex.org/W2046546139","https://openalex.org/W1992844569","https://openalex.org/W1999460937","https://openalex.org/W2113329733","https://openalex.org/W1918238777","https://openalex.org/W4285237197","https://openalex.org/W2341681417","https://openalex.org/W1984242440","https://openalex.org/W2613838074","https://openalex.org/W2133150343"],"abstract_inverted_index":{"BACKGROUND:":[0],"Stoichiometric":[1],"models":[2,26,52,236],"constitute":[3,248],"the":[4,11,21,28,47,106,120,152,158,189,195,202,240,258],"basic":[5],"framework":[6,81,210],"for":[7,27,38,53,74,215,231],"fluxome":[8,160],"quantification":[9,219],"in":[10,39,46,136,220,238,256],"realm":[12],"of":[13,23,30,49,60,110,157,184,197,242],"metabolic":[14,41,86,98,108,137,207,216,224,244,259],"engineering.":[15],"A":[16,79],"recurrent":[17],"bottleneck,":[18],"however,":[19],"is":[20,82,211],"establishment":[22],"consistent":[24],"stoichiometric":[25,51],"synthesis":[29],"recombinant":[31],"proteins":[32],"or":[33,222],"viruses.":[34],"Although":[35],"optimization":[36],"algorithms":[37],"silico":[40],"redesign":[42],"have":[43],"been":[44],"developed":[45],"context":[48],"genome-scale":[50],"small":[54],"molecule":[55],"production,":[56],"still":[57],"rudimentary":[58],"knowledge":[59],"how":[61],"different":[62],"cellular":[63,235],"levels":[64],"are":[65,237],"regulated":[66],"and":[67,123,218,228],"phenotypically":[68],"expressed":[69],"prevents":[70],"their":[71],"full":[72],"applicability":[73],"complex":[75],"product":[76],"optimization.":[77],"RESULTS:":[78],"hybrid":[80,176,206,243],"presented":[83],"combining":[84],"classical":[85],"flux":[87,208,245],"analysis":[88,209,246],"with":[89,100,130,151,261],"projection":[90],"to":[91,94,147,162,167,187,252],"latent":[92],"structures":[93],"further":[95],"link":[96],"estimated":[97,159],"fluxes":[99],"measured":[101],"productivities.":[102],"We":[103],"first":[104],"explore":[105],"functional":[107],"decomposition":[109],"a":[111,140,182,249],"baculovirus-producing":[112],"insect":[113],"cell":[114,263],"line":[115],"from":[116,154],"experimental":[117,227],"data,":[118],"highlighting":[119],"TCA":[121],"cycle":[122],"mitochondrial":[124],"respiration":[125],"as":[126],"pathways":[127],"strongly":[128],"associated":[129],"viral":[131],"replication.":[132],"To":[133],"reduce":[134],"uncertainty":[135],"target":[138],"identification,":[139],"Monte":[141],"Carlo":[142],"sampling":[143],"method":[144],"was":[145,178],"used":[146],"select":[148],"meaningful":[149],"associations":[150],"target,":[153],"which":[155],"66%":[156],"had":[161],"be":[163],"screened":[164],"out":[165],"due":[166],"weak":[168],"correlations":[169],"and/or":[170],"high":[171],"estimation":[172],"errors.":[173],"The":[174],"proposed":[175],"model":[177],"then":[179],"validated":[180],"using":[181],"subset":[183],"preliminary":[185],"experiments":[186],"pinpoint":[188],"same":[190],"determinant":[191],"pathways,":[192],"while":[193],"predicting":[194],"productivity":[196],"independent":[198],"cultures.":[199],"CONCLUSIONS:":[200],"Overall,":[201],"results":[203],"indicate":[204],"our":[205],"an":[212],"advantageous":[213],"tool":[214],"identification":[217],"incomplete":[221],"ill-defined":[223],"networks.":[225],"As":[226],"computational":[229,254],"solutions":[230],"constructing":[232],"comprehensive":[233],"global":[234],"development,":[239],"contribution":[241],"should":[247],"valuable":[250],"complement":[251],"current":[253],"platforms":[255],"bridging":[257],"state":[260],"improved":[262],"culture":[264],"performance.":[265]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":5}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}