{"id":"https://openalex.org/W2072543338","doi":"https://doi.org/10.1186/1752-0509-3-120","title":"Flux Design: In silico design of cell factories based on correlation of pathway fluxes to desired properties","display_name":"Flux Design: In silico design of cell factories based on correlation of pathway fluxes to desired properties","publication_year":2009,"publication_date":"2009-12-01","ids":{"openalex":"https://openalex.org/W2072543338","doi":"https://doi.org/10.1186/1752-0509-3-120","mag":"2072543338","pmid":"https://pubmed.ncbi.nlm.nih.gov/20035624"},"language":"en","primary_location":{"id":"doi:10.1186/1752-0509-3-120","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-3-120","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-3-120","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-3-120","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002106456","display_name":"Guido Melzer","orcid":"https://orcid.org/0000-0003-0081-5050"},"institutions":[{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Guido Melzer","raw_affiliation_strings":["Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gaussstr 17, 38106 Braunschweig, Germany. g.melzer@tu-bs.de","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gaussstr 17, 38106 Braunschweig, Germany. g.melzer@tu-bs.de","institution_ids":["https://openalex.org/I94509681"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany","institution_ids":["https://openalex.org/I94509681"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043809041","display_name":"Manely Eslahpazir Esfandabadi","orcid":null},"institutions":[{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Manely Eslahpazir Esfandabadi","raw_affiliation_strings":["Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","institution_ids":["https://openalex.org/I94509681"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany","institution_ids":["https://openalex.org/I94509681"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031893561","display_name":"Ezequiel Franco\u2010Lara","orcid":null},"institutions":[{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ezequiel Franco-Lara","raw_affiliation_strings":["Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","institution_ids":["https://openalex.org/I94509681"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany","institution_ids":["https://openalex.org/I94509681"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084674318","display_name":"Christoph Wittmann","orcid":"https://orcid.org/0000-0002-7952-985X"},"institutions":[{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Christoph Wittmann","raw_affiliation_strings":["Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemical Engineering, Technische Universit\u00e4t Braunschweig, Gau\u00dfstr 17, 38106, Braunschweig, Germany","institution_ids":["https://openalex.org/I94509681"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig  Braunschweig Germany","institution_ids":["https://openalex.org/I94509681"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5002106456"],"corresponding_institution_ids":["https://openalex.org/I94509681"],"apc_list":null,"apc_paid":null,"fwci":3.3783,"has_fulltext":true,"cited_by_count":87,"citation_normalized_percentile":{"value":0.92830437,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":100},"biblio":{"volume":"3","issue":"1","first_page":"120","last_page":"120"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9937999844551086,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9937999844551086,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10791","display_name":"Advanced Control Systems Optimization","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2207","display_name":"Control and Systems Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/in-silico","display_name":"In silico","score":0.8019307851791382},{"id":"https://openalex.org/keywords/systems-biology","display_name":"Systems biology","score":0.645104169845581},{"id":"https://openalex.org/keywords/flux","display_name":"Flux (metallurgy)","score":0.6115854382514954},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5111551880836487},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.43769511580467224},{"id":"https://openalex.org/keywords/biochemical-engineering","display_name":"Biochemical engineering","score":0.42702099680900574},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.3987036347389221},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.3810306191444397},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.23026397824287415},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.2141585648059845},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.15697813034057617}],"concepts":[{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.8019307851791382},{"id":"https://openalex.org/C152662350","wikidata":"https://www.wikidata.org/wiki/Q815297","display_name":"Systems biology","level":2,"score":0.645104169845581},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.6115854382514954},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5111551880836487},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.43769511580467224},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.42702099680900574},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3987036347389221},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3810306191444397},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.23026397824287415},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.2141585648059845},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.15697813034057617},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001234","descriptor_name":"Aspergillus niger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004851","descriptor_name":"Epoxide Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004851","descriptor_name":"Epoxide Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004851","descriptor_name":"Epoxide Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005087","descriptor_name":"Glucan 1,4-alpha-Glucosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005087","descriptor_name":"Glucan 1,4-alpha-Glucosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005087","descriptor_name":"Glucan 1,4-alpha-Glucosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D013213","descriptor_name":"Starch","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013213","descriptor_name":"Starch","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013213","descriptor_name":"Starch","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019295","descriptor_name":"Computational Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D048230","descriptor_name":"Corynebacterium glutamicum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049490","descriptor_name":"Systems Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D049490","descriptor_name":"Systems Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D049490","descriptor_name":"Systems Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1186/1752-0509-3-120","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-3-120","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-3-120","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},{"id":"pmid:20035624","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20035624","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC systems biology","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:2808316","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2808316","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Syst Biol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/1752-0509-3-120","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1752-0509-3-120","pdf_url":"https://bmcsystbiol.biomedcentral.com/counter/pdf/10.1186/1752-0509-3-120","source":{"id":"https://openalex.org/S92363890","display_name":"BMC Systems Biology","issn_l":"1752-0509","issn":["1752-0509"],"is_oa":true,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Systems Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G2290824390","display_name":null,"funder_award_id":"SFB 578","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2072543338.pdf","grobid_xml":"https://content.openalex.org/works/W2072543338.grobid-xml"},"referenced_works_count":55,"referenced_works":["https://openalex.org/W154635942","https://openalex.org/W565446105","https://openalex.org/W1002111426","https://openalex.org/W1485904186","https://openalex.org/W1534982905","https://openalex.org/W1549598114","https://openalex.org/W1793404797","https://openalex.org/W1969983517","https://openalex.org/W1975413073","https://openalex.org/W1980899764","https://openalex.org/W1998777096","https://openalex.org/W2004125187","https://openalex.org/W2012459591","https://openalex.org/W2023290064","https://openalex.org/W2023324052","https://openalex.org/W2031782630","https://openalex.org/W2032664706","https://openalex.org/W2046760236","https://openalex.org/W2053391580","https://openalex.org/W2055371288","https://openalex.org/W2065095031","https://openalex.org/W2065325476","https://openalex.org/W2072708674","https://openalex.org/W2073779101","https://openalex.org/W2077572824","https://openalex.org/W2079339133","https://openalex.org/W2083693802","https://openalex.org/W2089041533","https://openalex.org/W2090846925","https://openalex.org/W2091369078","https://openalex.org/W2091782040","https://openalex.org/W2091793452","https://openalex.org/W2093241068","https://openalex.org/W2094014683","https://openalex.org/W2099039198","https://openalex.org/W2100818174","https://openalex.org/W2102164505","https://openalex.org/W2110355460","https://openalex.org/W2119938020","https://openalex.org/W2121235957","https://openalex.org/W2123698848","https://openalex.org/W2131178894","https://openalex.org/W2134440848","https://openalex.org/W2134756070","https://openalex.org/W2135476401","https://openalex.org/W2155570608","https://openalex.org/W2157149907","https://openalex.org/W2159179186","https://openalex.org/W2161227644","https://openalex.org/W2161263507","https://openalex.org/W2184815744","https://openalex.org/W2192454018","https://openalex.org/W2222402790","https://openalex.org/W2411426692","https://openalex.org/W2498752631"],"related_works":["https://openalex.org/W2912862995","https://openalex.org/W3129816939","https://openalex.org/W2101501829","https://openalex.org/W2985427592","https://openalex.org/W2003194288","https://openalex.org/W2060263731","https://openalex.org/W391093122","https://openalex.org/W1971640599","https://openalex.org/W4306842392","https://openalex.org/W1969033266"],"abstract_inverted_index":{"BACKGROUND:":[0],"The":[1,122,318],"identification":[2,84,100],"of":[3,14,37,58,69,85,101,169,223,237,245,253,275,306],"genetic":[4,70,291],"target":[5,51,153],"genes":[6],"is":[7,24,155],"a":[8,26,35,67,107,152,162,235,268,343],"key":[9],"step":[10],"for":[11,81,186,293,333],"rational":[12],"engineering":[13,212,316,335],"production":[15,31,116,192,199,228,295],"strains":[16,117],"towards":[17,50,113],"bio-based":[18],"chemicals,":[19],"fuels":[20],"or":[21,90,144],"therapeutics.":[22],"This":[23],"often":[25],"difficult":[27],"task,":[28],"because":[29],"superior":[30],"performance":[32,193],"typically":[33],"requires":[34],"combination":[36],"multiple":[38],"targets,":[39,238],"whereby":[40],"the":[41,56,99,135,158,170,203,221,243,250,254,259,272,299,303,307],"complex":[42],"metabolic":[43,75,211,251,273,304,315,334],"networks":[44,274],"complicate":[45],"straightforward":[46],"identification.":[47],"Recent":[48],"attempts":[49],"prediction":[52,57],"mainly":[53],"focus":[54],"on":[55,166,249,258,298],"gene":[59],"deletion":[60,331],"targets":[61,86,102,138,205,247,292,332],"and":[62,175,194,256,279,289,302,330,340,352],"therefore":[63],"can":[64,321],"cover":[65],"only":[66],"part":[68],"modifications":[71],"proven":[72],"valuable":[73],"in":[74,78,161,191,200,229,313],"engineering.":[76],"Efficient":[77],"silico":[79],"methods":[80],"simultaneous":[82],"genome-scale":[83],"to":[87,106,139,157,179,325,346],"be":[88,140,311,322,347],"amplified":[89,141],"deleted":[91],"are":[92],"still":[93],"lacking.":[94],"RESULTS:":[95],"Here":[96],"we":[97,124],"propose":[98],"via":[103],"flux":[104,110,231,264],"correlation":[105,232,265],"chosen":[108],"objective":[109],"as":[111],"approach":[112],"improved":[114],"biotechnological":[115],"with":[118,208],"optimally":[119],"designed":[120],"fluxes.":[121,196],"approach,":[123],"name":[125],"Flux":[126],"Design,":[127],"computes":[128],"elementary":[129],"modes":[130,183],"and,":[131],"by":[132,149],"search":[133],"through":[134],"modes,":[136],"identifies":[137],"(positive":[142],"correlation)":[143],"down-regulated":[145],"(negative":[146],"correlation).":[147],"Supported":[148],"statistical":[150],"evaluation,":[151],"potential":[154],"attributed":[156],"identified":[159,204],"reactions":[160],"quantitative":[163],"manner.":[164],"Based":[165],"systems-wide":[167],"models":[168],"industrial":[171],"microorganisms":[172],"Corynebacterium":[173],"glutamicum":[174,202],"Aspergillus":[176],"niger,":[177],"up":[178],"more":[180],"than":[181],"20,000":[182],"were":[184],"obtained":[185],"each":[187],"case,":[188],"differing":[189],"strongly":[190],"intracellular":[195],"For":[197,226],"lysine":[198],"C.":[201],"nicely":[206],"matched":[207],"reported":[209],"successful":[210],"strategies.":[213,317],"In":[214],"addition,":[215],"simulations":[216],"revealed":[217],"insights,":[218],"e.g.":[219],"into":[220,271,349],"flexibility":[222],"energy":[224],"metabolism.":[225],"enzyme":[227],"A.niger":[230],"analysis":[233,266],"suggested":[234],"number":[236],"including":[239],"non-obvious":[240],"ones.":[241],"Hereby,":[242],"relevance":[244],"most":[246],"depended":[248],"state":[252,305],"cell":[255,308],"also":[257],"carbon":[260],"source.":[261],"CONCLUSIONS:":[262],"Objective":[263],"provided":[267],"detailed":[269],"insight":[270],"industrially":[276],"relevant":[277,290],"prokaryotic":[278],"eukaryotic":[280],"microorganisms.":[281],"It":[282],"was":[283],"shown":[284],"that":[285],"capacity,":[286],"pathway":[287],"usage,":[288],"optimal":[294],"partly":[296],"depend":[297],"network":[300],"structure":[301],"which":[309],"should":[310],"considered":[312],"future":[314],"presented":[319],"strategy":[320,345],"generally":[323],"used":[324],"identify":[326],"priority":[327],"sorted":[328],"amplification":[329],"purposes":[336],"under":[337],"various":[338],"conditions":[339],"thus":[341],"displays":[342],"useful":[344],"incorporated":[348],"efficient":[350],"strain":[351],"bioprocess":[353],"optimization.":[354]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":14},{"year":2012,"cited_by_count":13}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}