{"id":"https://openalex.org/W2057647571","doi":"https://doi.org/10.1186/1471-2105-9-368","title":"LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens","display_name":"LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens","publication_year":2008,"publication_date":"2008-09-10","ids":{"openalex":"https://openalex.org/W2057647571","doi":"https://doi.org/10.1186/1471-2105-9-368","mag":"2057647571","pmid":"https://pubmed.ncbi.nlm.nih.gov/18783594"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2105-9-368","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-368","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-368","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-368","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075641646","display_name":"Wah Heng Lee","orcid":null},"institutions":[{"id":"https://openalex.org/I165932596","display_name":"National University of Singapore","ror":"https://ror.org/01tgyzw49","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596"]},{"id":"https://openalex.org/I66068411","display_name":"Genome Institute of Singapore","ror":"https://ror.org/05k8wg936","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I66068411"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Wah Heng Lee","raw_affiliation_strings":["Genome Institute of Singapore, Genome, Singapore. leewhc@gis.a-star.edu.sg","Department of Computer Science, National University of Singapore, , Singapore","Genome Institute of Singapore, Genome, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore. leewhc@gis.a-star.edu.sg","institution_ids":["https://openalex.org/I66068411"]},{"raw_affiliation_string":"Department of Computer Science, National University of Singapore, , Singapore","institution_ids":["https://openalex.org/I165932596"]},{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103574732","display_name":"Christopher W. Wong","orcid":null},"institutions":[{"id":"https://openalex.org/I66068411","display_name":"Genome Institute of Singapore","ror":"https://ror.org/05k8wg936","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I66068411"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Christopher W Wong","raw_affiliation_strings":["Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","Genome Institute of Singapore, Genome, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]},{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021729143","display_name":"Wan Yee Leong","orcid":"https://orcid.org/0009-0003-7311-8245"},"institutions":[{"id":"https://openalex.org/I66068411","display_name":"Genome Institute of Singapore","ror":"https://ror.org/05k8wg936","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I66068411"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Wan Yee Leong","raw_affiliation_strings":["Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","Genome Institute of Singapore, Genome, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]},{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024804165","display_name":"Lance D. Miller","orcid":"https://orcid.org/0000-0003-3799-2528"},"institutions":[{"id":"https://openalex.org/I66068411","display_name":"Genome Institute of Singapore","ror":"https://ror.org/05k8wg936","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I66068411"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Lance D Miller","raw_affiliation_strings":["Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","Genome Institute of Singapore, Genome, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]},{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5012563463","display_name":"Wing\u2010Kin Sung","orcid":"https://orcid.org/0000-0001-7806-7086"},"institutions":[{"id":"https://openalex.org/I165932596","display_name":"National University of Singapore","ror":"https://ror.org/01tgyzw49","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596"]},{"id":"https://openalex.org/I66068411","display_name":"Genome Institute of Singapore","ror":"https://ror.org/05k8wg936","country_code":"SG","type":"facility","lineage":["https://openalex.org/I115228651","https://openalex.org/I2801752549","https://openalex.org/I66068411"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Wing Kin Sung","raw_affiliation_strings":["Department of Computer Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore","Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","Department of Computer Science, National University of Singapore, , Singapore","Genome Institute of Singapore, Genome, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Computer Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore","institution_ids":["https://openalex.org/I165932596"]},{"raw_affiliation_string":"Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]},{"raw_affiliation_string":"Department of Computer Science, National University of Singapore, , Singapore","institution_ids":["https://openalex.org/I165932596"]},{"raw_affiliation_string":"Genome Institute of Singapore, Genome, Singapore","institution_ids":["https://openalex.org/I66068411"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1690,"currency":"GBP","value_usd":2072},"apc_paid":{"value":1690,"currency":"GBP","value_usd":2072},"fwci":0.4212,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.64300015,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"9","issue":"1","first_page":"368","last_page":"368"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.4074000120162964,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.4074000120162964,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.3481999933719635,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.029500000178813934,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.6002576947212219},{"id":"https://openalex.org/keywords/dna-microarray","display_name":"DNA microarray","score":0.5574142932891846},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.5001118183135986},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.4536901116371155},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.35526707768440247},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.1472633183002472}],"concepts":[{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6002576947212219},{"id":"https://openalex.org/C95371953","wikidata":"https://www.wikidata.org/wiki/Q591745","display_name":"DNA microarray","level":4,"score":0.5574142932891846},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5001118183135986},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4536901116371155},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.35526707768440247},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1472633183002472},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016477","descriptor_name":"Artifacts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016477","descriptor_name":"Artifacts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016477","descriptor_name":"Artifacts","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1186/1471-2105-9-368","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-368","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-368","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmid:18783594","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18783594","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":null},{"id":"pmh:oai:scholarbank.nus.edu.sg:10635/39740","is_oa":false,"landing_page_url":"http://scholarbank.nus.edu.sg/handle/10635/39740","pdf_url":null,"source":{"id":"https://openalex.org/S7407052290","display_name":"National University of Singapore","issn_l":null,"issn":[],"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scopus","raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:331e736a78ea4dceb42cb28337f52da2","is_oa":true,"landing_page_url":"https://doaj.org/article/331e736a78ea4dceb42cb28337f52da2","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, Vol 9, Iss 1, p 368 (2008)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2553803","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2553803","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/1471-2105-9-368","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-368","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-368","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320696","display_name":"Agency for Science, Technology and Research","ror":"https://ror.org/036wvzt09"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2057647571.pdf","grobid_xml":"https://content.openalex.org/works/W2057647571.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W1559908984","https://openalex.org/W1576737979","https://openalex.org/W1613179247","https://openalex.org/W1905858949","https://openalex.org/W1924121735","https://openalex.org/W1970663985","https://openalex.org/W1980899588","https://openalex.org/W1996811680","https://openalex.org/W1998663222","https://openalex.org/W2007736853","https://openalex.org/W2024833332","https://openalex.org/W2029401821","https://openalex.org/W2037043224","https://openalex.org/W2037142940","https://openalex.org/W2048237985","https://openalex.org/W2107061522","https://openalex.org/W2116309501","https://openalex.org/W2117212863","https://openalex.org/W2119606140","https://openalex.org/W2123050502","https://openalex.org/W2130415678","https://openalex.org/W2130494035","https://openalex.org/W2133939379","https://openalex.org/W2137252440","https://openalex.org/W2138740534","https://openalex.org/W2141687278","https://openalex.org/W2153848419","https://openalex.org/W2158199481","https://openalex.org/W2169734644","https://openalex.org/W2170943623","https://openalex.org/W2173498175","https://openalex.org/W3196576386","https://openalex.org/W4205098749","https://openalex.org/W4247420482"],"related_works":["https://openalex.org/W2748952813","https://openalex.org/W2082860237","https://openalex.org/W2119695867","https://openalex.org/W2130076355","https://openalex.org/W1990804418","https://openalex.org/W1993764875","https://openalex.org/W2046158694","https://openalex.org/W2788277189","https://openalex.org/W2013243191","https://openalex.org/W2151865869"],"abstract_inverted_index":{"BACKGROUND:":[0],"Pathogen":[1],"detection":[2,20],"using":[3],"DNA":[4],"microarrays":[5],"has":[6],"the":[7,31,58,73,95,108,132,137,171],"potential":[8],"to":[9,90,119,166],"become":[10],"a":[11,41,69,77,82,146,156,160,167],"fast":[12],"and":[13,106],"comprehensive":[14],"diagnostics":[15],"tool.":[16],"However,":[17],"since":[18],"pathogen":[19,111,161],"chips":[21],"currently":[22],"utilize":[23],"random":[24,37,61,79,103,121,134,147],"primers":[25,29,122,135,175],"rather":[26],"than":[27],"specific":[28],"for":[30],"RT-PCR":[32],"step,":[33],"bias":[34],"inherent":[35],"in":[36,48,99,155],"PCR":[38,62,104,157],"amplification":[39,63,74,105,126,138],"becomes":[40],"serious":[42],"problem":[43],"that":[44,71,97,123,131],"causes":[45],"large":[46],"inaccuracies":[47],"hybridization":[49,65],"signals.":[50,66],"RESULTS:":[51],"In":[52],"this":[53],"paper,":[54],"we":[55,114],"study":[56],"how":[57],"efficiency":[59,75,139],"of":[60,76,94,101,110,145,173],"affects":[64],"We":[67],"describe":[68],"model":[70],"predicts":[72],"given":[78],"primer":[80,148],"on":[81,159],"target":[83],"viral":[84],"genome.":[85],"The":[86,142],"prediction":[87],"allows":[88],"us":[89],"filter":[91],"false-negative":[92],"probes":[93],"genome":[96],"lie":[98],"regions":[100],"poor":[102],"improves":[107],"accuracy":[109],"detection.":[112],"Subsequently,":[113],"propose":[115],"LOMA,":[116],"an":[117,177],"algorithm":[118],"generate":[120],"have":[124],"good":[125],"efficiency.":[127],"Wet-lab":[128],"validation":[129],"showed":[130],"generated":[133],"improve":[136],"significantly.":[140],"CONCLUSION:":[141],"blind":[143],"use":[144],"with":[149],"attached":[150],"universal":[151],"tag":[152],"(random-tagged":[153],"primer)":[154],"reaction":[158],"sample":[162],"may":[163],"not":[164],"lead":[165],"successful":[168],"amplification.":[169],"Thus,":[170],"design":[172],"random-tagged":[174],"is":[176],"important":[178],"consideration":[179],"when":[180],"performing":[181],"PCR.":[182]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-06-11T09:08:48.828518","created_date":"2025-10-10T00:00:00"}