{"id":"https://openalex.org/W2086910630","doi":"https://doi.org/10.1186/1471-2105-9-191","title":"Novel computational methods for increasing PCR primer design effectiveness in directed sequencing","display_name":"Novel computational methods for increasing PCR primer design effectiveness in directed sequencing","publication_year":2008,"publication_date":"2008-04-11","ids":{"openalex":"https://openalex.org/W2086910630","doi":"https://doi.org/10.1186/1471-2105-9-191","mag":"2086910630","pmid":"https://pubmed.ncbi.nlm.nih.gov/18405373"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2105-9-191","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-191","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-191","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-191","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101823033","display_name":"Kelvin Li","orcid":"https://orcid.org/0000-0003-3922-5664"},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kelvin Li","raw_affiliation_strings":["The J, Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA. kli@venterinstitute.org","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J, Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA. kli@venterinstitute.org","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002527404","display_name":"Anushka Brownley","orcid":null},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anushka Brownley","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047815294","display_name":"Timothy B. Stockwell","orcid":"https://orcid.org/0000-0002-3595-4539"},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Timothy B Stockwell","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072061647","display_name":"Karen Beeson","orcid":null},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen Beeson","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003454147","display_name":"Tina C. McIntosh","orcid":null},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tina C McIntosh","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083875825","display_name":"Dana Busam","orcid":null},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dana Busam","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001002097","display_name":"Steve Ferriera","orcid":null},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steve Ferriera","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000386688","display_name":"Sean D. Murphy","orcid":"https://orcid.org/0009-0000-4280-3694"},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sean Murphy","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5014460906","display_name":"Samuel L\u00e9vy","orcid":"https://orcid.org/0000-0001-7753-1043"},"institutions":[{"id":"https://openalex.org/I1301553790","display_name":"J. Craig Venter Institute","ror":"https://ror.org/049r1ts75","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1301553790"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samuel Levy","raw_affiliation_strings":["The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","The J. Craig Venter Institute, Rockville, USA"],"affiliations":[{"raw_affiliation_string":"The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD, 20850, USA","institution_ids":["https://openalex.org/I1301553790"]},{"raw_affiliation_string":"The J. Craig Venter Institute, Rockville, USA","institution_ids":["https://openalex.org/I1301553790"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5101823033"],"corresponding_institution_ids":["https://openalex.org/I1301553790"],"apc_list":{"value":1690,"currency":"GBP","value_usd":2072},"apc_paid":{"value":1690,"currency":"GBP","value_usd":2072},"fwci":0.965,"has_fulltext":true,"cited_by_count":38,"citation_normalized_percentile":{"value":0.74729352,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":100},"biblio":{"volume":"9","issue":"1","first_page":"191","last_page":"191"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9993000030517578,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9987000226974487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primer","display_name":"Primer (cosmetics)","score":0.7825703620910645},{"id":"https://openalex.org/keywords/amplicon","display_name":"Amplicon","score":0.7190215587615967},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.6450801491737366},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5658498406410217},{"id":"https://openalex.org/keywords/applications-of-pcr","display_name":"Applications of PCR","score":0.5657186508178711},{"id":"https://openalex.org/keywords/pipeline","display_name":"Pipeline (software)","score":0.5597699880599976},{"id":"https://openalex.org/keywords/in-silico-pcr","display_name":"In silico PCR","score":0.5442855358123779},{"id":"https://openalex.org/keywords/primer-dimer","display_name":"Primer dimer","score":0.505985677242279},{"id":"https://openalex.org/keywords/ensembl","display_name":"Ensembl","score":0.4812157452106476},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.3853123188018799},{"id":"https://openalex.org/keywords/polymerase-chain-reaction","display_name":"Polymerase chain reaction","score":0.37779441475868225},{"id":"https://openalex.org/keywords/genomics","display_name":"Genomics","score":0.33458074927330017},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.32342666387557983},{"id":"https://openalex.org/keywords/multiplex-polymerase-chain-reaction","display_name":"Multiplex polymerase chain reaction","score":0.20244640111923218},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.16074806451797485},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.15657344460487366}],"concepts":[{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7825703620910645},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.7190215587615967},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6450801491737366},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5658498406410217},{"id":"https://openalex.org/C28134486","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Applications of PCR","level":5,"score":0.5657186508178711},{"id":"https://openalex.org/C43521106","wikidata":"https://www.wikidata.org/wiki/Q2165493","display_name":"Pipeline (software)","level":2,"score":0.5597699880599976},{"id":"https://openalex.org/C200658500","wikidata":"https://www.wikidata.org/wiki/Q4112082","display_name":"In silico PCR","level":5,"score":0.5442855358123779},{"id":"https://openalex.org/C52001828","wikidata":"https://www.wikidata.org/wiki/Q2902493","display_name":"Primer dimer","level":5,"score":0.505985677242279},{"id":"https://openalex.org/C141674004","wikidata":"https://www.wikidata.org/wiki/Q1344256","display_name":"Ensembl","level":5,"score":0.4812157452106476},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3853123188018799},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.37779441475868225},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.33458074927330017},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.32342666387557983},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.20244640111923218},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16074806451797485},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.15657344460487366},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1186/1471-2105-9-191","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-191","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-191","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmid:18405373","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18405373","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":null},{"id":"pmh:oai:doaj.org/article:a188fddd0dff4af9a008b05de67285a0","is_oa":true,"landing_page_url":"https://doaj.org/article/a188fddd0dff4af9a008b05de67285a0","pdf_url":null,"source":{"id":"https://openalex.org/S112646816","display_name":"SHILAP Revista de lepidopterolog\u00eda","issn_l":"0300-5267","issn":["0300-5267","2340-4078"],"is_oa":true,"is_in_doaj":true,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, Vol 9, Iss 1, p 191 (2008)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:2396641","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2396641","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/1471-2105-9-191","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-9-191","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-9-191","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2086910630.pdf","grobid_xml":"https://content.openalex.org/works/W2086910630.grobid-xml"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W83140495","https://openalex.org/W1499500424","https://openalex.org/W1968982701","https://openalex.org/W1981101983","https://openalex.org/W1996423252","https://openalex.org/W2009031046","https://openalex.org/W2009095852","https://openalex.org/W2027098970","https://openalex.org/W2047846317","https://openalex.org/W2053691591","https://openalex.org/W2055043387","https://openalex.org/W2069516109","https://openalex.org/W2074929099","https://openalex.org/W2078028348","https://openalex.org/W2082931201","https://openalex.org/W2089667109","https://openalex.org/W2101102205","https://openalex.org/W2105086662","https://openalex.org/W2105192266","https://openalex.org/W2120627772","https://openalex.org/W2123332793","https://openalex.org/W2149410625","https://openalex.org/W4240237951"],"related_works":["https://openalex.org/W95394030","https://openalex.org/W85726586","https://openalex.org/W2922644496","https://openalex.org/W4311189471","https://openalex.org/W2319030365","https://openalex.org/W3049522820","https://openalex.org/W2064206660","https://openalex.org/W193921908","https://openalex.org/W2404129818","https://openalex.org/W1564541111"],"abstract_inverted_index":{"The":[0,28],"high-throughput":[1,74],"PCR":[2],"primer":[3,33,44,58,75],"design":[4,34,59,76],"pipeline":[5],"has":[6,36],"been":[7],"very":[8],"successful":[9,79],"in":[10],"providing":[11],"the":[12,32,52,57,63],"basis":[13],"for":[14,20,71],"high-quality":[15],"directed":[16,80],"sequencing":[17],"results":[18],"and":[19,26,73],"minimizing":[21],"costs":[22],"associated":[23],"with":[24,62],"labor":[25],"reprocessing.":[27],"modular":[29],"architecture":[30],"of":[31],"software":[35],"made":[37],"it":[38],"possible":[39],"to":[40,77],"readily":[41],"integrate":[42],"additional":[43],"critique":[45],"tests":[46],"based":[47],"on":[48],"iterative":[49],"feedback":[50],"from":[51],"laboratory.":[53],"As":[54],"a":[55,68],"result,":[56],"software,":[60],"coupled":[61],"laboratory":[64],"protocols,":[65],"serves":[66],"as":[67],"powerful":[69],"tool":[70],"low":[72],"enable":[78],"sequencing.":[81]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":3}],"updated_date":"2026-03-20T23:20:44.827607","created_date":"2025-10-10T00:00:00"}