{"id":"https://openalex.org/W2118854076","doi":"https://doi.org/10.1186/1471-2105-8-198","title":"A novel approach to sequence validating protein expression clones with automated decision making","display_name":"A novel approach to sequence validating protein expression clones with automated decision making","publication_year":2007,"publication_date":"2007-06-13","ids":{"openalex":"https://openalex.org/W2118854076","doi":"https://doi.org/10.1186/1471-2105-8-198","mag":"2118854076","pmid":"https://pubmed.ncbi.nlm.nih.gov/17567908"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2105-8-198","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-8-198","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-8-198","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-8-198","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054568887","display_name":"Elena Taycher","orcid":null},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210156643","display_name":"Harvard Global Health Institute","ror":"https://ror.org/055avk103","country_code":"US","type":"education","lineage":["https://openalex.org/I4210156643"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Elena Taycher","raw_affiliation_strings":["Harvard Institute of Proteomics, Harvard Medical School, Cambridge, MA 02141, USA. elena_taycher@hms.harvard.edu <elena_taycher@hms.harvard.edu>","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, MA 02141, USA. elena_taycher@hms.harvard.edu <elena_taycher@hms.harvard.edu>","institution_ids":["https://openalex.org/I4210156643","https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015001048","display_name":"Andreas Rolfs","orcid":null},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andreas Rolfs","raw_affiliation_strings":["Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025902765","display_name":"Yanhui Hu","orcid":"https://orcid.org/0000-0003-1494-1402"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yanhui Hu","raw_affiliation_strings":["Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071597724","display_name":"Dongmei Zuo","orcid":"https://orcid.org/0000-0001-8701-9238"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210134579","display_name":"Dana-Farber/Harvard Cancer Center","ror":"https://ror.org/03pvyf116","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210134579"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dongmei Zuo","raw_affiliation_strings":["DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I4210134579","https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085104422","display_name":"Stephanie E. Mohr","orcid":"https://orcid.org/0000-0001-9639-7708"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210134579","display_name":"Dana-Farber/Harvard Cancer Center","ror":"https://ror.org/03pvyf116","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210134579"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephanie E Mohr","raw_affiliation_strings":["DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I4210134579","https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041036396","display_name":"Janice Williamson","orcid":null},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Janice Williamson","raw_affiliation_strings":["Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046993686","display_name":"Joshua LaBaer","orcid":"https://orcid.org/0000-0001-5788-9697"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210134579","display_name":"Dana-Farber/Harvard Cancer Center","ror":"https://ror.org/03pvyf116","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210117453","https://openalex.org/I4210134579"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joshua LaBaer","raw_affiliation_strings":["DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA","Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, 320 Charles St., Cambridge, MA, 02141, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"DF/HCC DNA Resource Core, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I4210134579","https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Institute of Proteomics, Harvard Medical School, Cambridge, USA","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5046993686"],"corresponding_institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210134579"],"apc_list":{"value":1690,"currency":"GBP","value_usd":2072},"apc_paid":{"value":1690,"currency":"GBP","value_usd":2072},"fwci":0.533,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.66163658,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"8","issue":"1","first_page":"198","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8389999866485596,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.8389999866485596,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.02590000070631504,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.01889999955892563,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clone","display_name":"clone (Java method)","score":0.858035147190094},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6477731466293335},{"id":"https://openalex.org/keywords/contig","display_name":"Contig","score":0.5905495285987854},{"id":"https://openalex.org/keywords/software","display_name":"Software","score":0.5694339275360107},{"id":"https://openalex.org/keywords/sequence","display_name":"Sequence (biology)","score":0.5605745315551758},{"id":"https://openalex.org/keywords/set","display_name":"Set (abstract data type)","score":0.4998445510864258},{"id":"https://openalex.org/keywords/data-mining","display_name":"Data mining","score":0.4195694625377655},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.3500049114227295},{"id":"https://openalex.org/keywords/programming-language","display_name":"Programming language","score":0.3360818326473236},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.2502186894416809},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.17979788780212402},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.09075072407722473},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.08776503801345825}],"concepts":[{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.858035147190094},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6477731466293335},{"id":"https://openalex.org/C59582021","wikidata":"https://www.wikidata.org/wiki/Q1128751","display_name":"Contig","level":4,"score":0.5905495285987854},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.5694339275360107},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.5605745315551758},{"id":"https://openalex.org/C177264268","wikidata":"https://www.wikidata.org/wiki/Q1514741","display_name":"Set (abstract data type)","level":2,"score":0.4998445510864258},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.4195694625377655},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3500049114227295},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.3360818326473236},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2502186894416809},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17979788780212402},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.09075072407722473},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08776503801345825}],"mesh":[{"descriptor_ui":"D001185","descriptor_name":"Artificial Intelligence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001185","descriptor_name":"Artificial Intelligence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001185","descriptor_name":"Artificial Intelligence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D003661","descriptor_name":"Decision Support Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003661","descriptor_name":"Decision Support Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003661","descriptor_name":"Decision Support Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1186/1471-2105-8-198","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-8-198","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-8-198","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmid:17567908","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17567908","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":null},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.829.4211","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.829.4211","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"https://dash.harvard.edu/bitstream/handle/1/4739147/1914086.pdf?sequence%3D1","raw_type":"text"},{"id":"pmh:oai:dash.harvard.edu:1/4739147","is_oa":true,"landing_page_url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:4739147","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:4b583addecc64e249591cd0b3d98a313","is_oa":false,"landing_page_url":"https://doaj.org/article/4b583addecc64e249591cd0b3d98a313","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, Vol 8, Iss 1, p 198 (2007)","raw_type":"article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1914086","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1914086","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1186/1471-2105-8-198","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-8-198","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-8-198","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G1389899710","display_name":null,"funder_award_id":"R01HG003041","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G3836241885","display_name":null,"funder_award_id":"R01 HG003041","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2118854076.pdf","grobid_xml":"https://content.openalex.org/works/W2118854076.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W1499500424","https://openalex.org/W1528782506","https://openalex.org/W1939833584","https://openalex.org/W1971307410","https://openalex.org/W1981924974","https://openalex.org/W1985726486","https://openalex.org/W1996423252","https://openalex.org/W2043052104","https://openalex.org/W2044586667","https://openalex.org/W2055043387","https://openalex.org/W2063993421","https://openalex.org/W2072603644","https://openalex.org/W2074231493","https://openalex.org/W2104086373","https://openalex.org/W2109432249","https://openalex.org/W2113679691","https://openalex.org/W2119923823","https://openalex.org/W2121016876","https://openalex.org/W2121832839","https://openalex.org/W2125973903","https://openalex.org/W2128374023","https://openalex.org/W2134909038","https://openalex.org/W2135074566","https://openalex.org/W2137503836","https://openalex.org/W2137682295","https://openalex.org/W2153211479","https://openalex.org/W2155437662","https://openalex.org/W2155758168","https://openalex.org/W2158714788","https://openalex.org/W2159497035","https://openalex.org/W2162983919","https://openalex.org/W2166265186"],"related_works":["https://openalex.org/W2015535546","https://openalex.org/W2074687144","https://openalex.org/W2074936491","https://openalex.org/W1994449010","https://openalex.org/W1545755951","https://openalex.org/W2162517701","https://openalex.org/W140544075","https://openalex.org/W2043343084","https://openalex.org/W2069489638","https://openalex.org/W2360829643"],"abstract_inverted_index":{"BACKGROUND:":[0],"Whereas":[1],"the":[2,62,100,112,133,140,159,173,191,243,290,299,312,330],"molecular":[3],"assembly":[4],"of":[5,20,37,67,69,71,124,162,164,176,216,259,293,301,314,332],"protein":[6,58],"expression":[7,59],"clones":[8,22,146,177,217,228,288],"is":[9,23,39,75],"readily":[10],"automated":[11,244],"and":[12,30,82,135,200,209,236,251,303,310],"routinely":[13],"accomplished":[14],"in":[15,57,65,186],"high":[16,271],"throughput,":[17],"sequence":[18,50,106,120,138,193,274,316],"verification":[19,107,114,275],"these":[21],"still":[24],"largely":[25],"performed":[26],"manually,":[27],"an":[28,93],"arduous":[29],"time":[31,250,302],"consuming":[32],"process.":[33],"The":[34,277,295],"ultimate":[35],"goal":[36],"validation":[38,194],"to":[40,52,171,207,213,224,269,283,306,328,336],"determine":[41],"if":[42,204],"a":[43,76,122,130,221,240,256,260],"given":[44],"plasmid":[45,105],"clone":[46,87,113,119,134,210,263,273,308],"matches":[47],"its":[48,136],"reference":[49],"sufficiently":[51],"be":[53,151],"\"acceptable\"":[54],"for":[55,79,184,340],"use":[56,185],"experiments.":[60,188],"Given":[61],"accelerating":[63],"increase":[64],"availability":[66],"tens":[68],"thousands":[70,215],"unverified":[72],"clones,":[73],"there":[74],"strong":[77],"demand":[78],"rapid,":[80],"efficient":[81],"accurate":[83,254],"software":[84,108,278,296],"that":[85,157],"automates":[86,111],"validation.":[88],"RESULTS:":[89],"We":[90],"have":[91],"developed":[92],"Automated":[94],"Clone":[95],"Evaluation":[96],"(ACE)":[97],"system":[98],"-":[99],"first":[101],"comprehensive,":[102],"multi-platform,":[103],"web-based":[104],"package.":[109],"ACE":[110,189,219,247,266,326],"process":[115,195],"by":[116,246],"defining":[117],"each":[118,128,165],"as":[121,182],"list":[123,149],"multidimensional":[125],"discrepancy":[126],"objects,":[127],"describing":[129],"difference":[131],"between":[132],"expected":[137],"including":[139,196],"resulting":[141],"polypeptide":[142],"consequences.":[143],"To":[144],"evaluate":[145,284,307],"automatically,":[147],"this":[148],"can":[150],"compared":[152],"against":[153,178],"user":[154],"acceptance":[155,180,237],"criteria":[156,181],"specify":[158],"allowable":[160],"number":[161,313,331],"discrepancies":[163,205],"type.":[166],"This":[167],"strategy":[168],"allows":[169],"users":[170],"re-evaluate":[172],"same":[174],"set":[175],"different":[179],"needed":[183,335],"other":[187],"manages":[190],"entire":[192],"contig":[197],"management,":[198],"identifying":[199],"annotating":[201],"discrepancies,":[202,317],"determining":[203],"correspond":[206],"polymorphisms":[208],"finishing.":[211],"Designed":[212],"manage":[214],"simultaneously,":[218],"maintains":[220],"relational":[222],"database":[223],"store":[225],"information":[226],"about":[227],"at":[229,289],"various":[230],"completion":[231],"stages,":[232],"project":[233],"processing":[234],"parameters":[235],"criteria.":[238],"In":[239,324],"direct":[241],"comparison,":[242],"analysis":[245,258],"took":[248],"less":[249],"was":[252,267],"more":[253,285],"than":[255,286],"manual":[257,322],"93":[261],"gene":[262],"set.":[264],"CONCLUSION:":[265],"designed":[268],"facilitate":[270],"throughput":[272],"projects.":[276],"has":[279],"been":[280],"used":[281],"successfully":[282],"55,000":[287],"Harvard":[291],"Institute":[292],"Proteomics.":[294],"dramatically":[297],"reduced":[298],"amount":[300],"labor":[304],"required":[305],"sequences":[309],"decreased":[311],"missed":[315],"which":[318],"commonly":[319],"occur":[320],"during":[321],"evaluation.":[323],"addition,":[325],"helped":[327],"reduce":[329],"sequencing":[333],"reads":[334],"achieve":[337],"adequate":[338],"coverage":[339],"making":[341],"decisions":[342],"on":[343],"clones.":[344]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-05-21T06:26:12.895304","created_date":"2025-10-10T00:00:00"}