{"id":"https://openalex.org/W2954385317","doi":"https://doi.org/10.1145/3326172.3326178","title":"Advanced Single Cell Imaging Method Using Microfluidics and FUCCI to Determine Cell Cycle Progression and Cell Fate Decisions","display_name":"Advanced Single Cell Imaging Method Using Microfluidics and FUCCI to Determine Cell Cycle Progression and Cell Fate Decisions","publication_year":2019,"publication_date":"2019-03-28","ids":{"openalex":"https://openalex.org/W2954385317","doi":"https://doi.org/10.1145/3326172.3326178","mag":"2954385317"},"language":"en","primary_location":{"id":"doi:10.1145/3326172.3326178","is_oa":false,"landing_page_url":"https://doi.org/10.1145/3326172.3326178","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the 2019 9th International Conference on Biomedical Engineering and Technology","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103857500","display_name":"Ming Xi Jiang","orcid":null},"institutions":[{"id":"https://openalex.org/I3132008252","display_name":"Nanjing Foreign Language School","ror":"https://ror.org/03wvwbe92","country_code":"CN","type":"education","lineage":["https://openalex.org/I3132008252"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Ming Xi Jiang","raw_affiliation_strings":["Chong Qing Foreign Language School, Chong Qing, China"],"affiliations":[{"raw_affiliation_string":"Chong Qing Foreign Language School, Chong Qing, China","institution_ids":["https://openalex.org/I3132008252"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072448090","display_name":"Weichao Zhai","orcid":null},"institutions":[{"id":"https://openalex.org/I2801331674","display_name":"St James's University Hospital","ror":"https://ror.org/013s89d74","country_code":"GB","type":"healthcare","lineage":["https://openalex.org/I2799390153","https://openalex.org/I2801331674"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Weichao Zhai","raw_affiliation_strings":["Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK, St James University Hospital, Leeds, UK"],"affiliations":[{"raw_affiliation_string":"Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK, St James University Hospital, Leeds, UK","institution_ids":["https://openalex.org/I2801331674"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5103857500"],"corresponding_institution_ids":["https://openalex.org/I3132008252"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.05558396,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"31","last_page":"36"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.998199999332428,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.996399998664856,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microfluidics","display_name":"Microfluidics","score":0.6670839190483093},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"Live cell imaging","score":0.6480618715286255},{"id":"https://openalex.org/keywords/optical-imaging","display_name":"Optical imaging","score":0.5618046522140503},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.5385770201683044},{"id":"https://openalex.org/keywords/fluorescence-lifetime-imaging-microscopy","display_name":"Fluorescence-lifetime imaging microscopy","score":0.49469849467277527},{"id":"https://openalex.org/keywords/single-cell-analysis","display_name":"Single-cell analysis","score":0.48542603850364685},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.4534452557563782},{"id":"https://openalex.org/keywords/microscope","display_name":"Microscope","score":0.42317330837249756},{"id":"https://openalex.org/keywords/living-cell","display_name":"Living cell","score":0.4100513160228729},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.3839065432548523},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.28430330753326416},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.2239016592502594},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.19329500198364258},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.12920504808425903},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.09541657567024231}],"concepts":[{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.6670839190483093},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.6480618715286255},{"id":"https://openalex.org/C92630104","wikidata":"https://www.wikidata.org/wiki/Q4115103","display_name":"Optical imaging","level":2,"score":0.5618046522140503},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5385770201683044},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.49469849467277527},{"id":"https://openalex.org/C2776950831","wikidata":"https://www.wikidata.org/wiki/Q15635432","display_name":"Single-cell analysis","level":3,"score":0.48542603850364685},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4534452557563782},{"id":"https://openalex.org/C67649825","wikidata":"https://www.wikidata.org/wiki/Q196538","display_name":"Microscope","level":2,"score":0.42317330837249756},{"id":"https://openalex.org/C2984801823","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Living cell","level":2,"score":0.4100513160228729},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3839065432548523},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.28430330753326416},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2239016592502594},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.19329500198364258},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.12920504808425903},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09541657567024231},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1145/3326172.3326178","is_oa":false,"landing_page_url":"https://doi.org/10.1145/3326172.3326178","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the 2019 9th International Conference on Biomedical Engineering and Technology","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":0.5299999713897705}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1493710824","https://openalex.org/W1969488570","https://openalex.org/W1978372216","https://openalex.org/W1984804160","https://openalex.org/W1996435746","https://openalex.org/W2007951680","https://openalex.org/W2025241604","https://openalex.org/W2031633664","https://openalex.org/W2079832375","https://openalex.org/W2098708326","https://openalex.org/W2111202632","https://openalex.org/W2146428402","https://openalex.org/W2164528882","https://openalex.org/W2557216615"],"related_works":["https://openalex.org/W2043023303","https://openalex.org/W2069571516","https://openalex.org/W2062491242","https://openalex.org/W2016240992","https://openalex.org/W4285203576","https://openalex.org/W2028859130","https://openalex.org/W2042754901","https://openalex.org/W4401487630","https://openalex.org/W2465281819","https://openalex.org/W2615357881"],"abstract_inverted_index":{"Live":[0],"single-cell":[1,8,63],"imaging":[2,65,89,157,167],"has":[3],"emerged":[4],"as":[5],"an":[6],"advanced":[7],"study":[9],"tool":[10],"for":[11,115,155,169],"approaching":[12],"a":[13,62,102,152,161],"quantitative":[14],"understanding":[15],"of":[16,60,71,134],"many":[17],"biological":[18],"questions":[19],"in":[20,132],"recent":[21],"years.":[22],"In":[23,51],"previous":[24],"cell":[25,29,39,41,77,88,136,166],"studies":[26],"using":[27],"bulk":[28],"measurements,":[30],"the":[31,36,45,56,69,81,111,125,135],"population":[32],"averages":[33],"can":[34],"miss":[35],"information":[37],"from":[38],"to":[40,113,159],"variability":[42],"and":[43,49,67,105,128,142,163,172],"mask":[44],"underlying":[46],"signaling":[47],"networks":[48],"mechanisms.":[50],"this":[52,72],"paper,":[53],"I":[54],"address":[55],"key":[57],"design":[58],"considerations":[59],"developing":[61],"live":[64,165],"platform":[66,90,168],"demonstrate":[68],"capability":[70],"technology":[73],"through":[74,80],"analyzing":[75],"mES":[76],"life":[78],"cycle":[79],"FUCCI":[82],"system.":[83],"Overall,":[84],"our":[85,156],"semi-automated":[86],"single":[87],"not":[91,146],"only":[92],"offers":[93],"adjustable":[94],"intervals":[95],"between":[96],"fluorescent":[97],"imaging,":[98],"but":[99],"also":[100],"provides":[101],"constant":[103],"temperature":[104,140],"gas":[106,138],"feeding":[107],"devices":[108],"that":[109,122],"allows":[110],"cells":[112],"proliferate":[114],"extended":[116],"microscope":[117,126],"imaging.":[118],"Commercially":[119],"produced":[120],"incubators":[121],"fit":[123],"onto":[124],"stage":[127],"satisfied":[129],"all":[130],"requirements":[131],"restriction":[133],"movement,":[137],"feeding,":[139],"regulation":[141],"optical":[143],"accessibility":[144],"are":[145],"easily":[147],"available.":[148],"Thus,":[149],"there":[150],"exists":[151],"significant":[153],"potential":[154],"setup":[158],"provide":[160],"versatile":[162],"adaptable":[164],"both":[170],"academia":[171],"industrial":[173],"researchers.":[174]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}