{"id":"https://openalex.org/W2595933770","doi":"https://doi.org/10.1117/12.2254567","title":"A fully-automated multiscale kernel graph cuts based particle localization scheme for temporal focusing two-photon microscopy","display_name":"A fully-automated multiscale kernel graph cuts based particle localization scheme for temporal focusing two-photon microscopy","publication_year":2017,"publication_date":"2017-03-13","ids":{"openalex":"https://openalex.org/W2595933770","doi":"https://doi.org/10.1117/12.2254567","mag":"2595933770","pmid":"https://pubmed.ncbi.nlm.nih.gov/29276328"},"language":"en","primary_location":{"id":"doi:10.1117/12.2254567","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2254567","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033757017","display_name":"Xia Huang","orcid":"https://orcid.org/0000-0003-2321-5971"},"institutions":[{"id":"https://openalex.org/I164936912","display_name":"The University of Texas at El Paso","ror":"https://ror.org/04d5vba33","country_code":"US","type":"education","lineage":["https://openalex.org/I164936912"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xia Huang","raw_affiliation_strings":["The Univ. of Texas at El Paso (United States)"],"affiliations":[{"raw_affiliation_string":"The Univ. of Texas at El Paso (United States)","institution_ids":["https://openalex.org/I164936912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101642396","display_name":"Chunqiang Li","orcid":"https://orcid.org/0000-0003-1220-1611"},"institutions":[{"id":"https://openalex.org/I164936912","display_name":"The University of Texas at El Paso","ror":"https://ror.org/04d5vba33","country_code":"US","type":"education","lineage":["https://openalex.org/I164936912"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chunqiang Li","raw_affiliation_strings":["The Univ. of Texas at El Paso (United States)"],"affiliations":[{"raw_affiliation_string":"The Univ. of Texas at El Paso (United States)","institution_ids":["https://openalex.org/I164936912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090377305","display_name":"Chuan Xiao","orcid":"https://orcid.org/0000-0001-6599-8762"},"institutions":[{"id":"https://openalex.org/I164936912","display_name":"The University of Texas at El Paso","ror":"https://ror.org/04d5vba33","country_code":"US","type":"education","lineage":["https://openalex.org/I164936912"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chuan Xiao","raw_affiliation_strings":["The Univ. of Texas at El Paso (United States)"],"affiliations":[{"raw_affiliation_string":"The Univ. of Texas at El Paso (United States)","institution_ids":["https://openalex.org/I164936912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103092864","display_name":"Wenqing Sun","orcid":"https://orcid.org/0000-0002-0861-0941"},"institutions":[{"id":"https://openalex.org/I164936912","display_name":"The University of Texas at El Paso","ror":"https://ror.org/04d5vba33","country_code":"US","type":"education","lineage":["https://openalex.org/I164936912"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wenqing Sun","raw_affiliation_strings":["The Univ. of Texas at El Paso (United States)"],"affiliations":[{"raw_affiliation_string":"The Univ. of Texas at El Paso (United States)","institution_ids":["https://openalex.org/I164936912"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100374757","display_name":"Wei Qian","orcid":"https://orcid.org/0000-0002-9563-721X"},"institutions":[{"id":"https://openalex.org/I164936912","display_name":"The University of Texas at El Paso","ror":"https://ror.org/04d5vba33","country_code":"US","type":"education","lineage":["https://openalex.org/I164936912"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wei Qian","raw_affiliation_strings":["The Univ. of Texas at El Paso (United States)"],"affiliations":[{"raw_affiliation_string":"The Univ. of Texas at El Paso (United States)","institution_ids":["https://openalex.org/I164936912"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5033757017"],"corresponding_institution_ids":["https://openalex.org/I164936912"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.03375982,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"10137","issue":null,"first_page":"101371I","last_page":"101371I"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9991999864578247,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9991999864578247,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9957000017166138,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/kernel","display_name":"Kernel (algebra)","score":0.6298850774765015},{"id":"https://openalex.org/keywords/particle-filter","display_name":"Particle filter","score":0.615618109703064},{"id":"https://openalex.org/keywords/tracking","display_name":"Tracking (education)","score":0.5931338667869568},{"id":"https://openalex.org/keywords/kalman-filter","display_name":"Kalman filter","score":0.5582853555679321},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.5482776761054993},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.5465058088302612},{"id":"https://openalex.org/keywords/pixel","display_name":"Pixel","score":0.5462304353713989},{"id":"https://openalex.org/keywords/segmentation","display_name":"Segmentation","score":0.511459231376648},{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.5075646638870239},{"id":"https://openalex.org/keywords/graph","display_name":"Graph","score":0.5012071132659912},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.47636863589286804},{"id":"https://openalex.org/keywords/particle","display_name":"Particle (ecology)","score":0.4701680541038513},{"id":"https://openalex.org/keywords/noise","display_name":"Noise (video)","score":0.45192453265190125},{"id":"https://openalex.org/keywords/image-segmentation","display_name":"Image segmentation","score":0.44596588611602783},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.3782585859298706},{"id":"https://openalex.org/keywords/algorithm","display_name":"Algorithm","score":0.35018765926361084},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.3215966820716858},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.30100661516189575},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.22881633043289185},{"id":"https://openalex.org/keywords/image","display_name":"Image (mathematics)","score":0.13585513830184937}],"concepts":[{"id":"https://openalex.org/C74193536","wikidata":"https://www.wikidata.org/wiki/Q574844","display_name":"Kernel (algebra)","level":2,"score":0.6298850774765015},{"id":"https://openalex.org/C52421305","wikidata":"https://www.wikidata.org/wiki/Q1151499","display_name":"Particle filter","level":3,"score":0.615618109703064},{"id":"https://openalex.org/C2775936607","wikidata":"https://www.wikidata.org/wiki/Q466845","display_name":"Tracking (education)","level":2,"score":0.5931338667869568},{"id":"https://openalex.org/C157286648","wikidata":"https://www.wikidata.org/wiki/Q846780","display_name":"Kalman filter","level":2,"score":0.5582853555679321},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.5482776761054993},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.5465058088302612},{"id":"https://openalex.org/C160633673","wikidata":"https://www.wikidata.org/wiki/Q355198","display_name":"Pixel","level":2,"score":0.5462304353713989},{"id":"https://openalex.org/C89600930","wikidata":"https://www.wikidata.org/wiki/Q1423946","display_name":"Segmentation","level":2,"score":0.511459231376648},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5075646638870239},{"id":"https://openalex.org/C132525143","wikidata":"https://www.wikidata.org/wiki/Q141488","display_name":"Graph","level":2,"score":0.5012071132659912},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.47636863589286804},{"id":"https://openalex.org/C2778517922","wikidata":"https://www.wikidata.org/wiki/Q7140482","display_name":"Particle (ecology)","level":2,"score":0.4701680541038513},{"id":"https://openalex.org/C99498987","wikidata":"https://www.wikidata.org/wiki/Q2210247","display_name":"Noise (video)","level":3,"score":0.45192453265190125},{"id":"https://openalex.org/C124504099","wikidata":"https://www.wikidata.org/wiki/Q56933","display_name":"Image segmentation","level":3,"score":0.44596588611602783},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.3782585859298706},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.35018765926361084},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3215966820716858},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.30100661516189575},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.22881633043289185},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.13585513830184937},{"id":"https://openalex.org/C19417346","wikidata":"https://www.wikidata.org/wiki/Q7922","display_name":"Pedagogy","level":1,"score":0.0},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.0},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.0},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0},{"id":"https://openalex.org/C80444323","wikidata":"https://www.wikidata.org/wiki/Q2878974","display_name":"Theoretical computer science","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1117/12.2254567","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2254567","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},{"id":"pmid:29276328","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29276328","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of SPIE--the International Society for Optical Engineering","raw_type":null},{"id":"pmh:oai:europepmc.org:4650191","is_oa":false,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5737779","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1970535101","https://openalex.org/W1991875582","https://openalex.org/W2052250923","https://openalex.org/W2082823589","https://openalex.org/W2103706175","https://openalex.org/W2119300483","https://openalex.org/W2142916759","https://openalex.org/W2156568645","https://openalex.org/W2324834951","https://openalex.org/W6648348262","https://openalex.org/W6701155052"],"related_works":["https://openalex.org/W2015530857","https://openalex.org/W2556064263","https://openalex.org/W1991846142","https://openalex.org/W1583020711","https://openalex.org/W1521151968","https://openalex.org/W1580685205","https://openalex.org/W2128655648","https://openalex.org/W2900672867","https://openalex.org/W4252024964","https://openalex.org/W4253883008"],"abstract_inverted_index":{"The":[0],"temporal":[1],"focusing":[2],"two-photon":[3],"microscope":[4],"(TFM)":[5],"is":[6],"developed":[7,46],"to":[8,22,50],"perform":[9],"depth":[10],"resolved":[11],"wide":[12],"field":[13],"fluorescence":[14],"imaging":[15],"by":[16],"capturing":[17],"frames":[18],"sequentially.":[19],"However,":[20],"due":[21],"strong":[23],"nonignorable":[24],"noises":[25],"and":[26,82,86,96,116],"diffraction":[27],"rings":[28],"surrounding":[29],"particles,":[30],"further":[31],"researches":[32],"are":[33],"extremely":[34],"formidable":[35],"without":[36],"a":[37,47,67,76,87],"precise":[38],"particle":[39,72,91],"localization":[40],"technique.":[41],"In":[42],"this":[43],"paper,":[44],"we":[45],"fully-automated":[48],"scheme":[49,59],"locate":[51],"particles":[52,98,115,119],"positions":[53],"with":[54,103],"high":[55],"noise":[56,64],"tolerance.":[57],"Our":[58],"includes":[60],"the":[61],"following":[62],"procedures:":[63],"reduction":[65],"using":[66],"hybrid":[68],"Kalman":[69],"filter":[70],"method,":[71],"segmentation":[73,84],"based":[74,90],"on":[75,109],"multiscale":[77],"kernel":[78],"graph":[79],"cuts":[80],"global":[81],"local":[83],"algorithm,":[85],"kinematic":[88],"estimation":[89],"tracking":[92],"method.":[93],"Both":[94],"isolated":[95,114],"partial-overlapped":[97,118],"can":[99],"be":[100],"accurately":[101],"identified":[102],"removal":[104],"of":[105],"unrelated":[106],"pixels.":[107],"Based":[108],"our":[110],"quantitative":[111],"analysis,":[112],"96.22%":[113],"84.19%":[117],"were":[120],"successfully":[121],"detected.":[122]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}