{"id":"https://openalex.org/W1980311495","doi":"https://doi.org/10.1117/12.2081691","title":"Detecting cells in time varying intensity images in confocal microscopy for gene expression studies in living cells","display_name":"Detecting cells in time varying intensity images in confocal microscopy for gene expression studies in living cells","publication_year":2015,"publication_date":"2015-03-18","ids":{"openalex":"https://openalex.org/W1980311495","doi":"https://doi.org/10.1117/12.2081691","mag":"1980311495"},"language":"en","primary_location":{"id":"doi:10.1117/12.2081691","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2081691","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064391008","display_name":"Debasis Mitra","orcid":"https://orcid.org/0000-0002-4351-1252"},"institutions":[{"id":"https://openalex.org/I106959904","display_name":"Florida Institute of Technology","ror":"https://ror.org/04atsbb87","country_code":"US","type":"education","lineage":["https://openalex.org/I106959904"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Debasis Mitra","raw_affiliation_strings":["Florida Institute of Technology (United States)","Florida Institute of Technology, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Florida Institute of Technology (United States)","institution_ids":["https://openalex.org/I106959904"]},{"raw_affiliation_string":"Florida Institute of Technology, United States","institution_ids":["https://openalex.org/I106959904"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086373592","display_name":"Rostyslav Boutchko","orcid":null},"institutions":[{"id":"https://openalex.org/I148283060","display_name":"Lawrence Berkeley National Laboratory","ror":"https://ror.org/02jbv0t02","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I148283060","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rostyslav Boutchko","raw_affiliation_strings":["Lawrence Berkeley National Lab. (United States)","Lawrence Berkeley National Lab (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lawrence Berkeley National Lab. (United States)","institution_ids":["https://openalex.org/I148283060"]},{"raw_affiliation_string":"Lawrence Berkeley National Lab (United States)","institution_ids":["https://openalex.org/I148283060"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038342734","display_name":"Judhajeet Ray","orcid":"https://orcid.org/0000-0003-1524-2603"},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Judhajeet Ray","raw_affiliation_strings":["Iowa State Univ. (United States)","Iowa state University, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Iowa State Univ. (United States)","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Iowa state University, United States","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057520930","display_name":"Marit Nilsen\u2010Hamilton","orcid":"https://orcid.org/0000-0002-5496-1814"},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marit Nilsen-Hamilton","raw_affiliation_strings":["Iowa State Univ. (United States)","Iowa state University, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Iowa State Univ. (United States)","institution_ids":["https://openalex.org/I173911158"]},{"raw_affiliation_string":"Iowa state University, United States","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4087,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.80242614,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"9420","issue":null,"first_page":"942011","last_page":"942011"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9986000061035156,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/f\u00f6rster-resonance-energy-transfer","display_name":"F\u00f6rster resonance energy transfer","score":0.7588688731193542},{"id":"https://openalex.org/keywords/confocal","display_name":"Confocal","score":0.632204532623291},{"id":"https://openalex.org/keywords/population","display_name":"Population","score":0.5784751176834106},{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.562580406665802},{"id":"https://openalex.org/keywords/confocal-microscopy","display_name":"Confocal microscopy","score":0.5375764966011047},{"id":"https://openalex.org/keywords/fluorescence-microscope","display_name":"Fluorescence microscope","score":0.502587080001831},{"id":"https://openalex.org/keywords/gene-expression","display_name":"Gene expression","score":0.5011844635009766},{"id":"https://openalex.org/keywords/microscope","display_name":"Microscope","score":0.49903059005737305},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.3728938102722168},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.36648058891296387},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.34072035551071167},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.32171905040740967},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.26605623960494995},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.2634180188179016},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.245292067527771},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.14772331714630127}],"concepts":[{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.7588688731193542},{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.632204532623291},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5784751176834106},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.562580406665802},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.5375764966011047},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.502587080001831},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5011844635009766},{"id":"https://openalex.org/C67649825","wikidata":"https://www.wikidata.org/wiki/Q196538","display_name":"Microscope","level":2,"score":0.49903059005737305},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3728938102722168},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36648058891296387},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.34072035551071167},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.32171905040740967},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.26605623960494995},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.2634180188179016},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.245292067527771},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14772331714630127},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1117/12.2081691","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2081691","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},{"id":"pmh:oai:lib.dr.iastate.edu:bbmb_ag_conf-1000","is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/bbmb_ag_conf/1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry, Biophysics, and Molecular Biology Presentations and Posters","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy","score":0.7699999809265137}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W2037059908","https://openalex.org/W2094517427","https://openalex.org/W2106093304","https://openalex.org/W2122955765","https://openalex.org/W2151103935","https://openalex.org/W2170696666","https://openalex.org/W6611357420"],"related_works":["https://openalex.org/W3102476392","https://openalex.org/W4245158446","https://openalex.org/W2776588274","https://openalex.org/W2032337137","https://openalex.org/W2064110996","https://openalex.org/W2726111829","https://openalex.org/W2615020551","https://openalex.org/W4244928564","https://openalex.org/W4312294172","https://openalex.org/W1782140124"],"abstract_inverted_index":{"In":[0],"this":[1],"work":[2],"we":[3],"present":[4],"a":[5,29,39,59,123,166,182,194],"time-lapsed":[6,92],"confocal":[7,47],"microscopy":[8,48],"image":[9,125],"analysis":[10],"technique":[11],"for":[12,76,148,172],"an":[13,131],"automated":[14],"gene":[15,64,159],"expression":[16,160],"study":[17],"of":[18,42,49,85,91,113,117,175,178,207],"multiple":[19,146],"single":[20],"living":[21,183],"cells.":[22],"Fluorescence":[23],"Resonance":[24],"Energy":[25],"Transfer":[26],"(FRET)":[27],"is":[28,66,108],"technology":[30],"by":[31],"which":[32,138],"molecule-to-molecule":[33],"interactions":[34,188],"are":[35,74],"visualized.":[36],"We":[37,128],"analyzed":[38,96],"dynamic":[40],"series":[41],"~10<sup>2</sup>":[43],"images":[44,73],"obtained":[45],"using":[46],"fluorescence":[50],"in":[51,110,158,162,165,181,193,202],"yeast":[52],"cells":[53,164,190],"containing":[54],"RNA":[55],"reporters":[56],"that":[57,191],"give":[58],"FRET":[60],"signal":[61],"when":[62],"the":[63,81,86,114,118,140,170,176,187,199,203],"promoter":[65],"activated.":[67],"For":[68],"each":[69,99],"time":[70,103],"frame,":[71],"separate":[72],"available":[75],"three":[77],"spectral":[78],"channels":[79],"and":[80,104,111,186,205],"integrated":[82],"intensity":[83],"snapshot":[84],"system.":[87],"A":[88],"large":[89],"number":[90],"frames":[93],"must":[94],"be":[95],"to":[97,154],"identify":[98],"cell":[100,184],"individually":[101],"across":[102],"space,":[105],"as":[106,198],"it":[107,122],"moving":[109],"out":[112],"focal":[115],"plane":[116],"microscope.":[119],"This":[120],"makes":[121],"difficult":[124],"processing":[126],"problem.":[127],"have":[129],"proposed":[130],"algorithm":[132,144],"here,":[133],"based":[134],"on":[135],"scale-space":[136],"technique,":[137],"solves":[139],"problem":[141],"satisfactorily.":[142],"The":[143,152],"has":[145],"directions":[147],"even":[149],"further":[150],"improvement.":[151],"ability":[153],"rapidly":[155],"measure":[156],"changes":[157],"simultaneously":[161],"many":[163],"population":[167,185],"will":[168],"open":[169],"opportunity":[171],"real-time":[173],"studies":[174],"heterogeneity":[177],"genetic":[179],"response":[180],"between":[189],"occur":[192],"mixed":[195],"population,":[196],"such":[197],"ones":[200],"found":[201],"organs":[204],"tissues":[206],"multicellular":[208],"organisms.":[209]},"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-06-11T09:08:48.828518","created_date":"2025-10-10T00:00:00"}
