{"id":"https://openalex.org/W4293117604","doi":"https://doi.org/10.1109/robio54168.2021.9739468","title":"Deep-Learning-Based Detection of Neurons for Two-Photon Imaging Patch Clamp System in vivo","display_name":"Deep-Learning-Based Detection of Neurons for Two-Photon Imaging Patch Clamp System in vivo","publication_year":2021,"publication_date":"2021-12-27","ids":{"openalex":"https://openalex.org/W4293117604","doi":"https://doi.org/10.1109/robio54168.2021.9739468"},"language":"en","primary_location":{"id":"doi:10.1109/robio54168.2021.9739468","is_oa":false,"landing_page_url":"https://doi.org/10.1109/robio54168.2021.9739468","pdf_url":null,"source":{"id":"https://openalex.org/S4363607846","display_name":"2021 IEEE International Conference on Robotics and Biomimetics (ROBIO)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2021 IEEE International Conference on Robotics and Biomimetics (ROBIO)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100738962","display_name":"Jie Li","orcid":"https://orcid.org/0000-0002-4430-7608"},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Jie Li","raw_affiliation_strings":["Nankai University,College of Artificial Intelligence,Tianjin,China","College of Artificial Intelligence, Nankai University, Tianjin, China","Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China"],"affiliations":[{"raw_affiliation_string":"Nankai University,College of Artificial Intelligence,Tianjin,China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"College of Artificial Intelligence, Nankai University, Tianjin, China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China","institution_ids":["https://openalex.org/I205237279"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039388105","display_name":"Liangpeng Wei","orcid":"https://orcid.org/0000-0002-7921-4532"},"institutions":[{"id":"https://openalex.org/I5740404","display_name":"Tianjin Medical University","ror":"https://ror.org/02mh8wx89","country_code":"CN","type":"education","lineage":["https://openalex.org/I5740404"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Liangpeng Wei","raw_affiliation_strings":["Tianjin Medical University,School of Biomedical Engineering and Technology,Tianjin,China","School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Tianjin Medical University,School of Biomedical Engineering and Technology,Tianjin,China","institution_ids":["https://openalex.org/I5740404"]},{"raw_affiliation_string":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, China","institution_ids":["https://openalex.org/I5740404"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090578099","display_name":"Qili Zhao","orcid":"https://orcid.org/0000-0002-9517-7460"},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qili Zhao","raw_affiliation_strings":["Nankai University,College of Artificial Intelligence,Tianjin,China","College of Artificial Intelligence, Nankai University, Tianjin, China","Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China"],"affiliations":[{"raw_affiliation_string":"Nankai University,College of Artificial Intelligence,Tianjin,China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"College of Artificial Intelligence, Nankai University, Tianjin, China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China","institution_ids":["https://openalex.org/I205237279"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101923674","display_name":"Mingzhu Sun","orcid":"https://orcid.org/0000-0003-0562-7665"},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mingzhu Sun","raw_affiliation_strings":["Nankai University,College of Artificial Intelligence,Tianjin,China","College of Artificial Intelligence, Nankai University, Tianjin, China","Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China"],"affiliations":[{"raw_affiliation_string":"Nankai University,College of Artificial Intelligence,Tianjin,China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"College of Artificial Intelligence, Nankai University, Tianjin, China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China","institution_ids":["https://openalex.org/I205237279"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062876714","display_name":"Hui Shen","orcid":"https://orcid.org/0000-0002-1500-4560"},"institutions":[{"id":"https://openalex.org/I5740404","display_name":"Tianjin Medical University","ror":"https://ror.org/02mh8wx89","country_code":"CN","type":"education","lineage":["https://openalex.org/I5740404"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hui Shen","raw_affiliation_strings":["Tianjin Medical University,School of Biomedical Engineering and Technology,Tianjin,China","School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Tianjin Medical University,School of Biomedical Engineering and Technology,Tianjin,China","institution_ids":["https://openalex.org/I5740404"]},{"raw_affiliation_string":"School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, China","institution_ids":["https://openalex.org/I5740404"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101672156","display_name":"Xin Zhao","orcid":"https://orcid.org/0000-0002-2978-0595"},"institutions":[{"id":"https://openalex.org/I205237279","display_name":"Nankai University","ror":"https://ror.org/01y1kjr75","country_code":"CN","type":"education","lineage":["https://openalex.org/I205237279"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xin Zhao","raw_affiliation_strings":["Nankai University,College of Artificial Intelligence,Tianjin,China","College of Artificial Intelligence, Nankai University, Tianjin, China","Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China"],"affiliations":[{"raw_affiliation_string":"Nankai University,College of Artificial Intelligence,Tianjin,China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"College of Artificial Intelligence, Nankai University, Tianjin, China","institution_ids":["https://openalex.org/I205237279"]},{"raw_affiliation_string":"Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, China","institution_ids":["https://openalex.org/I205237279"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5100738962"],"corresponding_institution_ids":["https://openalex.org/I205237279"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.63675214,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1485","last_page":"1490"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.991599977016449,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9882000088691711,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6892009973526001},{"id":"https://openalex.org/keywords/clamp","display_name":"Clamp","score":0.5583114624023438},{"id":"https://openalex.org/keywords/patch-clamp","display_name":"Patch clamp","score":0.5112509727478027},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.4883994460105896},{"id":"https://openalex.org/keywords/pipette","display_name":"Pipette","score":0.4612845778465271},{"id":"https://openalex.org/keywords/biomedical-engineering","display_name":"Biomedical engineering","score":0.4477095305919647},{"id":"https://openalex.org/keywords/two-photon-excitation-microscopy","display_name":"Two-photon excitation microscopy","score":0.4447666108608246},{"id":"https://openalex.org/keywords/neuron","display_name":"Neuron","score":0.441190242767334},{"id":"https://openalex.org/keywords/clamping","display_name":"Clamping","score":0.4344802796840668},{"id":"https://openalex.org/keywords/automation","display_name":"Automation","score":0.41021743416786194},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.3444901704788208},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.2607845664024353},{"id":"https://openalex.org/keywords/neuroscience","display_name":"Neuroscience","score":0.24327218532562256},{"id":"https://openalex.org/keywords/electrophysiology","display_name":"Electrophysiology","score":0.22805994749069214},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.18928870558738708},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.13041117787361145},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.11416932940483093},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.11070266366004944}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6892009973526001},{"id":"https://openalex.org/C2776161997","wikidata":"https://www.wikidata.org/wiki/Q846600","display_name":"Clamp","level":3,"score":0.5583114624023438},{"id":"https://openalex.org/C147944092","wikidata":"https://www.wikidata.org/wiki/Q176366","display_name":"Patch clamp","level":3,"score":0.5112509727478027},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.4883994460105896},{"id":"https://openalex.org/C151552171","wikidata":"https://www.wikidata.org/wiki/Q163373","display_name":"Pipette","level":2,"score":0.4612845778465271},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.4477095305919647},{"id":"https://openalex.org/C47785574","wikidata":"https://www.wikidata.org/wiki/Q373859","display_name":"Two-photon excitation microscopy","level":3,"score":0.4447666108608246},{"id":"https://openalex.org/C2778794669","wikidata":"https://www.wikidata.org/wiki/Q43054","display_name":"Neuron","level":2,"score":0.441190242767334},{"id":"https://openalex.org/C84111939","wikidata":"https://www.wikidata.org/wiki/Q5125465","display_name":"Clamping","level":2,"score":0.4344802796840668},{"id":"https://openalex.org/C115901376","wikidata":"https://www.wikidata.org/wiki/Q184199","display_name":"Automation","level":2,"score":0.41021743416786194},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.3444901704788208},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2607845664024353},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.24327218532562256},{"id":"https://openalex.org/C185263204","wikidata":"https://www.wikidata.org/wiki/Q1154774","display_name":"Electrophysiology","level":2,"score":0.22805994749069214},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.18928870558738708},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13041117787361145},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.11416932940483093},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.11070266366004944},{"id":"https://openalex.org/C78519656","wikidata":"https://www.wikidata.org/wiki/Q101333","display_name":"Mechanical engineering","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/robio54168.2021.9739468","is_oa":false,"landing_page_url":"https://doi.org/10.1109/robio54168.2021.9739468","pdf_url":null,"source":{"id":"https://openalex.org/S4363607846","display_name":"2021 IEEE International Conference on Robotics and Biomimetics (ROBIO)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2021 IEEE International Conference on Robotics and Biomimetics (ROBIO)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2022341425","https://openalex.org/W2149437626","https://openalex.org/W3036944672","https://openalex.org/W2375338697","https://openalex.org/W4231525483","https://openalex.org/W2020386169","https://openalex.org/W1683412264","https://openalex.org/W2384001192","https://openalex.org/W2365506001","https://openalex.org/W50277463"],"abstract_inverted_index":{"Targeting":[0],"a":[1,35,78,89,108,120,129],"neuron":[2,36,53,83],"is":[3,47],"an":[4],"essential":[5],"and":[6,30,55,145,177],"crucial":[7],"procedure":[8],"in":[9,12,19,20,40,85,160],"patch-clamp":[10,150],"recording":[11],"vivo.":[13],"While":[14],"considerable":[15],"levels":[16,176],"of":[17,71,92,124,132,143,170],"automation":[18,46],"vivo":[21,161],"patch":[22,162,172,181],"clamp":[23,173],"has":[24],"recently":[25],"been":[26],"achieved,":[27],"manually":[28],"selecting":[29],"close":[31],"attention":[32],"to":[33,81,102,110,148,157],"track":[34],"are":[37,66],"normally":[38],"involved":[39],"the":[41,68,72,112,168],"procedure.":[42],"Coupling":[43],"this":[44,136],"with":[45,94,174],"difficult":[48],"because":[49],"micropipette":[50],"penetration":[51],"induces":[52],"deformation":[54],"displacement.":[56],"And":[57,164],"neurons":[58,93,144,158],"can":[59,97,139,153,166],"only":[60],"be":[61,98],"clearly":[62],"observed":[63],"when":[64],"they":[65],"on":[67,128],"focal":[69],"plane":[70],"two-photon":[73],"microscopy.":[74],"Here,":[75],"we":[76,106],"report":[77],"deep-learning-based":[79],"framework":[80],"realize":[82],"detection":[84,122],"vivo,":[86],"by":[87],"which":[88,141,146],"topological":[90],"map":[91],"three-dimensional":[95],"coordinate":[96],"obtained.":[99],"In":[100],"order":[101],"train":[103],"our":[104,117],"model,":[105],"collect":[107],"dataset":[109],"feed":[111],"network.":[113],"Experiments":[114],"show":[115],"that":[116],"method":[118],"achieves":[119],"satisfying":[121],"accuracy":[123],"more":[125],"than":[126],"90%":[127],"testing":[130],"set":[131],"500":[133],"images.":[134],"With":[135],"technique,":[137],"operators":[138],"personalize":[140],"type":[142],"one":[147],"obtain":[149],"recording.":[151],"It":[152],"help":[154],"facilitate":[155],"access":[156],"for":[159],"clamping.":[163],"it":[165],"improve":[167],"development":[169],"automatic":[171],"advanced":[175],"also":[178],"benefit":[179],"manual":[180],"clamp.":[182]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}