{"id":"https://openalex.org/W2104802208","doi":"https://doi.org/10.1109/bmei.2009.5305096","title":"Isolation and Analysis of Differential Expression Genes on Common Carp Peripheral Leucocyte Stimulated and Non-Stimulated with Mitogen by DDRT-PCR","display_name":"Isolation and Analysis of Differential Expression Genes on Common Carp Peripheral Leucocyte Stimulated and Non-Stimulated with Mitogen by DDRT-PCR","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2104802208","doi":"https://doi.org/10.1109/bmei.2009.5305096","mag":"2104802208"},"language":"en","primary_location":{"id":"doi:10.1109/bmei.2009.5305096","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2009.5305096","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 2nd International Conference on Biomedical Engineering and Informatics","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044702317","display_name":"Lianrui Li","orcid":"https://orcid.org/0009-0003-8912-405X"},"institutions":[{"id":"https://openalex.org/I194450716","display_name":"Jilin University","ror":"https://ror.org/00js3aw79","country_code":"CN","type":"education","lineage":["https://openalex.org/I194450716"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Lianrui Li","raw_affiliation_strings":["Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China","Key Lab. for Zoonosis Res., Jilin Univ., Changchun, China"],"affiliations":[{"raw_affiliation_string":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China","institution_ids":["https://openalex.org/I194450716"]},{"raw_affiliation_string":"Key Lab. for Zoonosis Res., Jilin Univ., Changchun, China","institution_ids":["https://openalex.org/I194450716"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028224186","display_name":"Qiang L\u00fc","orcid":"https://orcid.org/0000-0003-4889-5299"},"institutions":[{"id":"https://openalex.org/I194450716","display_name":"Jilin University","ror":"https://ror.org/00js3aw79","country_code":"CN","type":"education","lineage":["https://openalex.org/I194450716"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiang Lu","raw_affiliation_strings":["Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China","Key Lab. for Zoonosis Res., Jilin Univ., Changchun, China"],"affiliations":[{"raw_affiliation_string":"Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China","institution_ids":["https://openalex.org/I194450716"]},{"raw_affiliation_string":"Key Lab. for Zoonosis Res., Jilin Univ., Changchun, China","institution_ids":["https://openalex.org/I194450716"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084234953","display_name":"Haiyang Gu","orcid":"https://orcid.org/0000-0002-0701-9033"},"institutions":[{"id":"https://openalex.org/I3129293551","display_name":"Tarim University","ror":"https://ror.org/05202v862","country_code":"CN","type":"education","lineage":["https://openalex.org/I3129293551"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Haiyang Gu","raw_affiliation_strings":["College of Life Science, Tarim University, Alar, China","College of Life Science, Tarim University,Alar,China"],"affiliations":[{"raw_affiliation_string":"College of Life Science, Tarim University, Alar, China","institution_ids":["https://openalex.org/I3129293551"]},{"raw_affiliation_string":"College of Life Science, Tarim University,Alar,China","institution_ids":["https://openalex.org/I3129293551"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100756918","display_name":"Lijun Xu","orcid":"https://orcid.org/0000-0001-9515-6128"},"institutions":[{"id":"https://openalex.org/I3129293551","display_name":"Tarim University","ror":"https://ror.org/05202v862","country_code":"CN","type":"education","lineage":["https://openalex.org/I3129293551"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lijun Xu","raw_affiliation_strings":["College of Life Science, Tarim University, Alar, China","College of Life Science, Tarim University,Alar,China"],"affiliations":[{"raw_affiliation_string":"College of Life Science, Tarim University, Alar, China","institution_ids":["https://openalex.org/I3129293551"]},{"raw_affiliation_string":"College of Life Science, Tarim University,Alar,China","institution_ids":["https://openalex.org/I3129293551"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081023644","display_name":"Genyuan Chen","orcid":"https://orcid.org/0000-0002-9231-3842"},"institutions":[{"id":"https://openalex.org/I3129293551","display_name":"Tarim University","ror":"https://ror.org/05202v862","country_code":"CN","type":"education","lineage":["https://openalex.org/I3129293551"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Genyuan Chen","raw_affiliation_strings":["College of Life Science, Tarim University, Alar, China","College of Life Science, Tarim University,Alar,China"],"affiliations":[{"raw_affiliation_string":"College of Life Science, Tarim University, Alar, China","institution_ids":["https://openalex.org/I3129293551"]},{"raw_affiliation_string":"College of Life Science, Tarim University,Alar,China","institution_ids":["https://openalex.org/I3129293551"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102144237","display_name":"Wenbin Zuo","orcid":null},"institutions":[{"id":"https://openalex.org/I3129293551","display_name":"Tarim University","ror":"https://ror.org/05202v862","country_code":"CN","type":"education","lineage":["https://openalex.org/I3129293551"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenbin Zuo","raw_affiliation_strings":["College of Life Science, Tarim University, Alar, China","College of Life Science, Tarim University,Alar,China"],"affiliations":[{"raw_affiliation_string":"College of Life Science, Tarim University, Alar, China","institution_ids":["https://openalex.org/I3129293551"]},{"raw_affiliation_string":"College of Life Science, Tarim University,Alar,China","institution_ids":["https://openalex.org/I3129293551"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5044702317"],"corresponding_institution_ids":["https://openalex.org/I194450716"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.16831089,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"45","issue":null,"first_page":"1","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.9991000294685364,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10506","display_name":"Aquaculture disease management and microbiota","score":0.9991000294685364,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.9991000294685364,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11403","display_name":"Invertebrate Immune Response Mechanisms","score":0.9983999729156494,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/molecular-biology","display_name":"Molecular biology","score":0.6877428293228149},{"id":"https://openalex.org/keywords/complementary-dna","display_name":"Complementary DNA","score":0.648838222026825},{"id":"https://openalex.org/keywords/genbank","display_name":"GenBank","score":0.5711002945899963},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.5538288354873657},{"id":"https://openalex.org/keywords/differential-display","display_name":"Differential display","score":0.5458231568336487},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.515067994594574},{"id":"https://openalex.org/keywords/open-reading-frame","display_name":"Open reading frame","score":0.48465949296951294},{"id":"https://openalex.org/keywords/gene-expression","display_name":"Gene expression","score":0.4484824240207672},{"id":"https://openalex.org/keywords/peptide-sequence","display_name":"Peptide sequence","score":0.29750820994377136},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.21354880928993225}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6877428293228149},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.648838222026825},{"id":"https://openalex.org/C79029880","wikidata":"https://www.wikidata.org/wiki/Q901755","display_name":"GenBank","level":3,"score":0.5711002945899963},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5538288354873657},{"id":"https://openalex.org/C2776657240","wikidata":"https://www.wikidata.org/wiki/Q5275341","display_name":"Differential display","level":4,"score":0.5458231568336487},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.515067994594574},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.48465949296951294},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4484824240207672},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.29750820994377136},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.21354880928993225}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/bmei.2009.5305096","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2009.5305096","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 2nd International Conference on Biomedical Engineering and Informatics","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W164079534","https://openalex.org/W1990858735","https://openalex.org/W2045276302","https://openalex.org/W2060655074","https://openalex.org/W2090377361","https://openalex.org/W2132181266","https://openalex.org/W2132524516","https://openalex.org/W2139818147","https://openalex.org/W2144318866","https://openalex.org/W2165296283","https://openalex.org/W2165977100","https://openalex.org/W2167501366"],"related_works":["https://openalex.org/W4388299304","https://openalex.org/W2358933002","https://openalex.org/W3143391977","https://openalex.org/W2001848758","https://openalex.org/W3150559971","https://openalex.org/W2393887791","https://openalex.org/W3141223289","https://openalex.org/W2393371103","https://openalex.org/W1494783489","https://openalex.org/W241967109"],"abstract_inverted_index":{"DDRT-PCR":[0,27],"is":[1],"an":[2],"effective":[3],"and":[4,20,40,46,57,72,83,93,123,148],"quick":[5],"method":[6],"to":[7,30,61,153,155],"study":[8,25],"differentially":[9],"expressed":[10],"genes":[11],"on":[12],"the":[13,79,98,139,149],"same":[14],"cells":[15],"under":[16],"differently":[17],"physiological":[18],"status":[19],"different":[21,49,70],"stages.":[22],"In":[23],"this":[24],"fluorescence":[26],"was":[28,81,135,151],"used":[29],"compare":[31],"mRNA":[32],"of":[33,36,74,91,100,160],"peripheral":[34],"leucocytes":[35],"Common":[37],"Carp":[38],"stimulated":[39],"non-stimulated":[41],"by":[42],"mitogens":[43],"(LPA,":[44],"ConA":[45],"PHA)":[47],"in":[48],"times":[50],"such":[51],"as":[52],"4":[53],"h,":[54],"12":[55],"h":[56],"24":[58],"h.":[59],"According":[60],"bioinformatic":[62,94],"technology":[63],"it":[64,67],"indicated":[65],"that":[66,97],"had":[68,127],"92":[69],"fragments":[71,86],"87":[73],"them":[75],"were":[76,87,105],"re-amplified.":[77],"After":[78],"gene":[80],"cloned":[82],"sequenced,":[84],"60":[85],"identified.":[88],"The":[89,130],"analysis":[90],"sequence":[92],"information":[95],"revealed":[96],"products":[99],"5":[101],"cDNA":[102,134],"fragments,":[103],"which":[104],"highly":[106],"homologous":[107],"with":[108,138],"thymosin":[109,132],"beta":[110,116,133],",":[111],"proteasome":[112],"activator":[113],"complex":[114],"PA28":[115],"subunit,":[117],"CXC":[118],"chemokine,":[119],"translation":[120],"elongation":[121],"factor-lbeta(EF-lbeta)":[122],"matrix":[124],"metalloproteinase":[125],"(MMPs)":[126],"immune":[128],"response.":[129],"full-length":[131],"528":[136],"bp":[137],"open":[140],"reading":[141],"frame":[142],"encoding":[143],"46":[144],"amino":[145],"acid":[146],"residues,":[147],"sequences":[150],"submitted":[152],"GenBank":[154],"gain":[156],"a":[157],"accession":[158],"number":[159],"AY457946.":[161]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}