{"id":"https://openalex.org/W3108605953","doi":"https://doi.org/10.1109/tbme.2020.3040337","title":"In Vitro Experimental and Numerical Studies on the Preferential Ablation of Chemo-Resistant Tumor Cells Induced by High-Voltage Nanosecond Pulsed Electric Fields","display_name":"In Vitro Experimental and Numerical Studies on the Preferential Ablation of Chemo-Resistant Tumor Cells Induced by High-Voltage Nanosecond Pulsed Electric Fields","publication_year":2020,"publication_date":"2020-11-25","ids":{"openalex":"https://openalex.org/W3108605953","doi":"https://doi.org/10.1109/tbme.2020.3040337","mag":"3108605953","pmid":"https://pubmed.ncbi.nlm.nih.gov/33232222"},"language":"en","primary_location":{"id":"doi:10.1109/tbme.2020.3040337","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tbme.2020.3040337","pdf_url":null,"source":{"id":"https://openalex.org/S5240358","display_name":"IEEE Transactions on Biomedical Engineering","issn_l":"0018-9294","issn":["0018-9294","1558-2531"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Biomedical Engineering","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100376985","display_name":"Hongmei Liu","orcid":"https://orcid.org/0000-0001-5193-9462"},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Hongmei Liu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-5193-9462","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087988000","display_name":"Chenguo Yao","orcid":"https://orcid.org/0000-0002-1781-2756"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chenguo Yao","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1781-2756","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060978725","display_name":"Yajun Zhao","orcid":"https://orcid.org/0000-0003-3029-0291"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yajun Zhao","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3029-0291","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100408254","display_name":"Xiaoling Chen","orcid":"https://orcid.org/0000-0002-5153-2547"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiaoling Chen","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5153-2547","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060589907","display_name":"Shoulong Dong","orcid":"https://orcid.org/0000-0001-6104-0282"},"institutions":[{"id":"https://openalex.org/I158842170","display_name":"Chongqing University","ror":"https://ror.org/023rhb549","country_code":"CN","type":"education","lineage":["https://openalex.org/I158842170"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shoulong Dong","raw_affiliation_strings":["State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing, China"],"raw_orcid":"https://orcid.org/0000-0001-6104-0282","affiliations":[{"raw_affiliation_string":"State Key Laboratory of Power Transmission Equipment and System Security and New Technology, Chongqing University, Chongqing, China","institution_ids":["https://openalex.org/I158842170"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100336266","display_name":"Li Wang","orcid":"https://orcid.org/0000-0003-4959-0729"},"institutions":[{"id":"https://openalex.org/I151075929","display_name":"Army Medical University","ror":"https://ror.org/05w21nn13","country_code":"CN","type":"education","lineage":["https://openalex.org/I151075929"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Wang","raw_affiliation_strings":["Institute of Immunology PLA and Department of Immunology, Army Medical University (Third Military Medical University), Chongqing, China"],"raw_orcid":"https://orcid.org/0000-0003-4959-0729","affiliations":[{"raw_affiliation_string":"Institute of Immunology PLA and Department of Immunology, Army Medical University (Third Military Medical University), Chongqing, China","institution_ids":["https://openalex.org/I151075929"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027511386","display_name":"Rafael V. Davalos","orcid":"https://orcid.org/0000-0003-1503-9509"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rafael V. Davalos","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1503-9509","affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5100376985"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.787,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.83556987,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":99},"biblio":{"volume":"68","issue":"8","first_page":"2400","last_page":"2411"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.9947999715805054,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.9947999715805054,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10642","display_name":"Plasma Applications and Diagnostics","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11920","display_name":"Pulsed Power Technology Applications","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2207","display_name":"Control and Systems Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/electroporation","display_name":"Electroporation","score":0.8287577629089355},{"id":"https://openalex.org/keywords/irreversible-electroporation","display_name":"Irreversible electroporation","score":0.7589751482009888},{"id":"https://openalex.org/keywords/viability-assay","display_name":"Viability assay","score":0.6167559623718262},{"id":"https://openalex.org/keywords/biophysics","display_name":"Biophysics","score":0.5493027567863464},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.4886382222175598},{"id":"https://openalex.org/keywords/ablation","display_name":"Ablation","score":0.48597609996795654},{"id":"https://openalex.org/keywords/intracellular","display_name":"Intracellular","score":0.4703429341316223},{"id":"https://openalex.org/keywords/cancer-research","display_name":"Cancer research","score":0.4232163727283478},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.4140196144580841},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.4069826006889343},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.21289560198783875},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.20324492454528809},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.12789708375930786}],"concepts":[{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.8287577629089355},{"id":"https://openalex.org/C2778716261","wikidata":"https://www.wikidata.org/wiki/Q17164846","display_name":"Irreversible electroporation","level":4,"score":0.7589751482009888},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.6167559623718262},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5493027567863464},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4886382222175598},{"id":"https://openalex.org/C2778902805","wikidata":"https://www.wikidata.org/wiki/Q322177","display_name":"Ablation","level":2,"score":0.48597609996795654},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.4703429341316223},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4232163727283478},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4140196144580841},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4069826006889343},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.21289560198783875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20324492454528809},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12789708375930786},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004560","descriptor_name":"Electricity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004560","descriptor_name":"Electricity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004560","descriptor_name":"Electricity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":true},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":true},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000628","qualifier_name":"therapy","is_major_topic":true},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1109/tbme.2020.3040337","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tbme.2020.3040337","pdf_url":null,"source":{"id":"https://openalex.org/S5240358","display_name":"IEEE Transactions on Biomedical Engineering","issn_l":"0018-9294","issn":["0018-9294","1558-2531"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Biomedical Engineering","raw_type":"journal-article"},{"id":"pmid:33232222","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33232222","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE transactions on bio-medical engineering","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8100000023841858}],"awards":[{"id":"https://openalex.org/G2567514699","display_name":null,"funder_award_id":"51877022","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G2948337013","display_name":null,"funder_award_id":"51807016","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G934361122","display_name":null,"funder_award_id":"2019CDCGDQ317","funder_id":"https://openalex.org/F4320335787","funder_display_name":"Fundamental Research Funds for the Central Universities"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320335787","display_name":"Fundamental Research Funds for the Central Universities","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":73,"referenced_works":["https://openalex.org/W112026335","https://openalex.org/W627627653","https://openalex.org/W628139540","https://openalex.org/W1481421933","https://openalex.org/W1512927198","https://openalex.org/W1521859708","https://openalex.org/W1597938223","https://openalex.org/W1931094976","https://openalex.org/W1932344038","https://openalex.org/W1965896778","https://openalex.org/W1967650453","https://openalex.org/W1972708469","https://openalex.org/W1973838853","https://openalex.org/W1977540990","https://openalex.org/W1983784258","https://openalex.org/W1995453310","https://openalex.org/W2004973808","https://openalex.org/W2012550889","https://openalex.org/W2013485244","https://openalex.org/W2014974514","https://openalex.org/W2026667284","https://openalex.org/W2033455306","https://openalex.org/W2033580055","https://openalex.org/W2043729132","https://openalex.org/W2046449631","https://openalex.org/W2047860376","https://openalex.org/W2057929059","https://openalex.org/W2060040620","https://openalex.org/W2062475588","https://openalex.org/W2069494822","https://openalex.org/W2069825136","https://openalex.org/W2072759354","https://openalex.org/W2078175688","https://openalex.org/W2079530087","https://openalex.org/W2089985908","https://openalex.org/W2099580531","https://openalex.org/W2103805973","https://openalex.org/W2104408549","https://openalex.org/W2114144028","https://openalex.org/W2118967271","https://openalex.org/W2126867444","https://openalex.org/W2132473980","https://openalex.org/W2137554605","https://openalex.org/W2138045682","https://openalex.org/W2141606387","https://openalex.org/W2146526868","https://openalex.org/W2154290132","https://openalex.org/W2170454539","https://openalex.org/W2172501680","https://openalex.org/W2238653099","https://openalex.org/W2272984102","https://openalex.org/W2286554493","https://openalex.org/W2344880335","https://openalex.org/W2485164715","https://openalex.org/W2556616425","https://openalex.org/W2566109146","https://openalex.org/W2606109717","https://openalex.org/W2768278937","https://openalex.org/W2768738060","https://openalex.org/W2889646458","https://openalex.org/W2896382962","https://openalex.org/W2911188335","https://openalex.org/W2942750823","https://openalex.org/W2994323246","https://openalex.org/W3024549659","https://openalex.org/W4231216647","https://openalex.org/W4243218694","https://openalex.org/W4244904354","https://openalex.org/W4247555605","https://openalex.org/W4254021855","https://openalex.org/W6604491461","https://openalex.org/W6640414814","https://openalex.org/W6695642976"],"related_works":["https://openalex.org/W4254021855","https://openalex.org/W1499022678","https://openalex.org/W2328152481","https://openalex.org/W197636502","https://openalex.org/W2186342673","https://openalex.org/W2945460259","https://openalex.org/W4394821614","https://openalex.org/W2973234734","https://openalex.org/W2523682967","https://openalex.org/W2110949835"],"abstract_inverted_index":{"Chemoresistance":[0],"causes":[1],"tumor":[2,21,34,126,195],"recurrence":[3],"and":[4,11,14,85,117,131,160,176,203,215],"metastasis,":[5],"resulting":[6],"in":[7,219],"poor":[8],"clinical":[9],"outcomes":[10],"low":[12],"survival,":[13],"has":[15],"been":[16],"considered":[17],"an":[18,48,201],"obstacle":[19],"to":[20,40,67,93,122,171],"therapy.":[22],"The":[23,61,76,129],"development":[24],"of":[25,63,71,88,189,209,222],"novel":[26,223],"therapeutic":[27,52],"approaches":[28],"that":[29,135,184],"can":[30,148],"effectively":[31],"kill":[32],"chemoresistant":[33,102],"cells":[35,103,127],"(CRTCs)":[36],"is":[37,66],"therefore":[38],"critical":[39],"overcoming":[41],"these":[42],"obstacles.":[43],"OBJECTIVE:":[44],"Here,":[45],"we":[46],"introduce":[47],"emerging":[49],"physical":[50],"feature-based":[51],"approach":[53],"based":[54],"on":[55,73,81,164,168],"nanosecond":[56],"pulsed":[57],"electric":[58],"fields":[59],"(nsPEFs).":[60],"goal":[62],"this":[64,144],"study":[65,199,208],"investigate":[68],"the":[69,165,207,220],"effect":[70],"nsPEFs":[72,89,136,147,185],"CRTCs.":[74],"METHODS:":[75],"cell":[77,108,177],"viability,":[78],"ablation":[79,115,119,188],"effects":[80,152],"a":[82,106],"3D-cultured":[83],"scaffold,":[84],"lethal":[86],"thresholds":[87],"were":[90],"evaluated":[91],"according":[92],"fluorescence":[94,162],"staining":[95],"assays.":[96],"RESULTS:":[97],"nsPEF":[98],"treatment":[99],"preferentially":[100],"affected":[101],"(A549/CDDP)":[104],"with":[105],"higher":[107,150,154],"viability":[109],"inhibition":[110],"ability/cell":[111],"death":[112],"rate,":[113],"larger":[114,157,173],"area,":[116,159],"lower":[118],"threshold":[120],"compared":[121],"their":[123,172,192],"respective":[124,193],"homologous":[125,194],"(A549).":[128],"experimental":[130,202],"theoretical":[132,204],"studies":[133],"suggested":[134],"displayed":[137],"selective":[138,145],"behavior":[139],"toward":[140],"intracellular":[141],"structures.":[142],"With":[143],"character,":[146],"induce":[149],"electroporation":[151,158],"(e.g.,":[153],"pore":[155],"number,":[156],"faster":[161],"dissipation":[163],"nuclear":[166,174],"envelope)":[167],"CRTCs":[169,190,211],"due":[170],"size":[175],"membrane":[178],"capacitance.":[179],"CONCLUSION:":[180],"These":[181],"findings":[182],"demonstrated":[183],"induced":[186],"preferential":[187],"over":[191],"cells.":[196],"SIGNIFICANCE:":[197],"This":[198],"provides":[200],"basis":[205],"for":[206],"killing":[210],"by":[212],"electrical":[213],"treatments":[214],"suggests":[216],"potential":[217],"applications":[218],"optimization":[221],"anti-chemoresistance":[224],"methods.":[225]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":2}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}