{"id":"https://openalex.org/W3033921359","doi":"https://doi.org/10.1109/tbme.2020.2999896","title":"Zebrafish Larva Orientation and Smooth Aspiration Control for Microinjection","display_name":"Zebrafish Larva Orientation and Smooth Aspiration Control for Microinjection","publication_year":2020,"publication_date":"2020-06-04","ids":{"openalex":"https://openalex.org/W3033921359","doi":"https://doi.org/10.1109/tbme.2020.2999896","mag":"3033921359","pmid":"https://pubmed.ncbi.nlm.nih.gov/32746018"},"language":"en","primary_location":{"id":"doi:10.1109/tbme.2020.2999896","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tbme.2020.2999896","pdf_url":null,"source":{"id":"https://openalex.org/S5240358","display_name":"IEEE Transactions on Biomedical Engineering","issn_l":"0018-9294","issn":["0018-9294","1558-2531"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Biomedical Engineering","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044462891","display_name":"Gefei Zhang","orcid":"https://orcid.org/0000-0001-6971-2418"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Gefei Zhang","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":"https://orcid.org/0000-0001-6971-2418","affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056969596","display_name":"Mingsi Tong","orcid":"https://orcid.org/0000-0002-1939-0040"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mingsi Tong","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology, Harbin, China"],"raw_orcid":"https://orcid.org/0000-0002-1939-0040","affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology, Harbin, China","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103848909","display_name":"Songlin Zhuang","orcid":"https://orcid.org/0000-0003-3072-0634"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Songlin Zhuang","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":"https://orcid.org/0000-0003-3072-0634","affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044251375","display_name":"Xinghu Yu","orcid":"https://orcid.org/0000-0001-8181-6199"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xinghu Yu","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100726954","display_name":"Weichao Sun","orcid":"https://orcid.org/0000-0001-6837-3821"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weichao Sun","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":"https://orcid.org/0000-0001-6837-3821","affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024993361","display_name":"Weiyang Lin","orcid":"https://orcid.org/0000-0002-0493-1289"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weiyang Lin","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5036684787","display_name":"Huijun Gao","orcid":"https://orcid.org/0000-0001-5554-5452"},"institutions":[{"id":"https://openalex.org/I204983213","display_name":"Harbin Institute of Technology","ror":"https://ror.org/01yqg2h08","country_code":"CN","type":"education","lineage":["https://openalex.org/I204983213"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huijun Gao","raw_affiliation_strings":["Research Institute of Intelligent Control and Systems, Harbin Institute of Technology"],"raw_orcid":"https://orcid.org/0000-0001-5554-5452","affiliations":[{"raw_affiliation_string":"Research Institute of Intelligent Control and Systems, Harbin Institute of Technology","institution_ids":["https://openalex.org/I204983213"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I204983213"],"apc_list":null,"apc_paid":null,"fwci":2.408,"has_fulltext":false,"cited_by_count":30,"citation_normalized_percentile":{"value":0.8855076,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"68","issue":"1","first_page":"47","last_page":"55"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.8700000047683716,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.8700000047683716,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11486","display_name":"Micro and Nano Robotics","score":0.03200000151991844,"subfield":{"id":"https://openalex.org/subfields/3104","display_name":"Condensed Matter Physics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11170","display_name":"Biomimetic flight and propulsion mechanisms","score":0.025200000032782555,"subfield":{"id":"https://openalex.org/subfields/2202","display_name":"Aerospace Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microinjection","display_name":"Microinjection","score":0.8357059955596924},{"id":"https://openalex.org/keywords/zebrafish","display_name":"Zebrafish","score":0.71994948387146},{"id":"https://openalex.org/keywords/orientation","display_name":"Orientation (vector space)","score":0.5575112700462341},{"id":"https://openalex.org/keywords/anatomy","display_name":"Anatomy","score":0.502302885055542},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.4527474045753479},{"id":"https://openalex.org/keywords/larva","display_name":"Larva","score":0.4225618839263916},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.34364622831344604},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.33278775215148926},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.10841229557991028},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.09162834286689758},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.0797995924949646},{"id":"https://openalex.org/keywords/ecology","display_name":"Ecology","score":0.07596489787101746}],"concepts":[{"id":"https://openalex.org/C62525196","wikidata":"https://www.wikidata.org/wiki/Q3311945","display_name":"Microinjection","level":2,"score":0.8357059955596924},{"id":"https://openalex.org/C2776878037","wikidata":"https://www.wikidata.org/wiki/Q169444","display_name":"Zebrafish","level":3,"score":0.71994948387146},{"id":"https://openalex.org/C16345878","wikidata":"https://www.wikidata.org/wiki/Q107472979","display_name":"Orientation (vector space)","level":2,"score":0.5575112700462341},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.502302885055542},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4527474045753479},{"id":"https://openalex.org/C173758957","wikidata":"https://www.wikidata.org/wiki/Q129270","display_name":"Larva","level":2,"score":0.4225618839263916},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34364622831344604},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.33278775215148926},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10841229557991028},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.09162834286689758},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0797995924949646},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.07596489787101746},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007814","descriptor_name":"Larva","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007814","descriptor_name":"Larva","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007814","descriptor_name":"Larva","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1109/tbme.2020.2999896","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tbme.2020.2999896","pdf_url":null,"source":{"id":"https://openalex.org/S5240358","display_name":"IEEE Transactions on Biomedical Engineering","issn_l":"0018-9294","issn":["0018-9294","1558-2531"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Biomedical Engineering","raw_type":"journal-article"},{"id":"pmid:32746018","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32746018","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE transactions on bio-medical engineering","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G6049830159","display_name":"\u57fa\u4e8e\u5fae\u7eb3\u7c73\u673a\u5668\u4eba\u6280\u672f\u7684\u7ec6\u80de\u5e76\u884c\u64cd\u63a7\u53ca\u591a\u7ef4\u7269\u7406\u4fe1\u606f\u68c0\u6d4b\u7814\u7a76","funder_award_id":"61933008","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320327001","display_name":"State Key Laboratory of Robotics and System","ror":null},{"id":"https://openalex.org/F4320329133","display_name":"Key Laboratory of Micro-Systems and Micro-Structures Manufacturing, Ministry of Education, Harbin Institute of Technology","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1487127700","https://openalex.org/W1508154674","https://openalex.org/W1980699864","https://openalex.org/W1981172646","https://openalex.org/W1981239255","https://openalex.org/W1989100148","https://openalex.org/W1995063639","https://openalex.org/W2000970397","https://openalex.org/W2006136808","https://openalex.org/W2014012238","https://openalex.org/W2061549490","https://openalex.org/W2092932703","https://openalex.org/W2096755293","https://openalex.org/W2104839086","https://openalex.org/W2106200559","https://openalex.org/W2116553446","https://openalex.org/W2120481893","https://openalex.org/W2125349610","https://openalex.org/W2135046321","https://openalex.org/W2170111436","https://openalex.org/W2278041753","https://openalex.org/W2288384663","https://openalex.org/W2318603155","https://openalex.org/W2433282201","https://openalex.org/W2542126794","https://openalex.org/W2572293562","https://openalex.org/W2606303170","https://openalex.org/W2771718748","https://openalex.org/W2796149604","https://openalex.org/W2898871035","https://openalex.org/W2903927617","https://openalex.org/W2908480305","https://openalex.org/W2920513656","https://openalex.org/W6680056290"],"related_works":["https://openalex.org/W2473584654","https://openalex.org/W3129642199","https://openalex.org/W2944413083","https://openalex.org/W4256528904","https://openalex.org/W1597543017","https://openalex.org/W2157877758","https://openalex.org/W4293058866","https://openalex.org/W4245153001","https://openalex.org/W2730292983","https://openalex.org/W1983023387"],"abstract_inverted_index":{"OBJECTIVE:":[0],"The":[1,78,134,149],"zebrafish":[2,25,31,41,60,75,143],"has":[3],"been":[4],"proven":[5],"to":[6,128],"be":[7,124],"a":[8,58,84,100],"significant":[9],"model":[10,102],"organism":[11],"in":[12,19,68,83],"various":[13],"research":[14],"fields.":[15],"For":[16],"investigating":[17],"the":[18,69,90,96,153],"vivo":[20],"properties":[21],"of":[22,103,139,155],"biologics":[23],"within":[24],"with":[26,64],"developed":[27],"organs,":[28],"an":[29,109],"automated":[30],"larva":[32,42,61,91,104,129],"organ":[33],"injection":[34],"system":[35],"is":[36,81,106,113,131,137],"crucially":[37],"needed.":[38],"However,":[39],"current":[40],"manipulation":[43,62],"techniques":[44],"cannot":[45],"accomplish":[46],"this":[47,54],"operation":[48],"efficiently":[49],"and":[50,73,108,126,141,146,160],"continuously.":[51],"METHODS:":[52],"In":[53],"paper,":[55],"we":[56],"present":[57],"novel":[59],"technique":[63],"two":[65],"key":[66],"steps":[67],"microinjection":[70],"system:":[71],"orienting":[72,140],"aspirating":[74,142],"larvae":[76,144],"automatically.":[77],"orientation":[79],"control":[80],"realized":[82],"customized":[85],"microfluidic":[86],"chip,":[87],"after":[88],"which":[89],"moves":[92],"tail-first":[93],"until":[94],"reaching":[95],"channel":[97],"exit.":[98],"Then":[99],"dynamic":[101],"aspiration":[105],"established":[107],"adaptive":[110],"robust":[111],"controller":[112],"designed.":[114],"RESULTS:":[115],"Experimental":[116],"results":[117],"demonstrate":[118],"that":[119],"high":[120],"success":[121],"rate":[122],"can":[123],"reached":[125],"damage":[127],"body":[130],"reduced.":[132],"CONCLUSION:":[133],"presented":[135],"strategy":[136],"capable":[138],"smoothly":[145],"efficiently.":[147],"SIGNIFICANCE:":[148],"proposed":[150],"methods":[151],"have":[152],"advantage":[154],"low":[156],"cost,":[157],"easy":[158],"implementability":[159],"good":[161],"stability.":[162]},"counts_by_year":[{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}