{"id":"https://openalex.org/W4406856755","doi":"https://doi.org/10.1109/tase.2025.3533657","title":"A Practical Micropipette-Image Calibration Method for Somatic Cell Microinjection","display_name":"A Practical Micropipette-Image Calibration Method for Somatic Cell Microinjection","publication_year":2025,"publication_date":"2025-01-01","ids":{"openalex":"https://openalex.org/W4406856755","doi":"https://doi.org/10.1109/tase.2025.3533657"},"language":"en","primary_location":{"id":"doi:10.1109/tase.2025.3533657","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tase.2025.3533657","pdf_url":null,"source":{"id":"https://openalex.org/S34881539","display_name":"IEEE Transactions on Automation Science and Engineering","issn_l":"1545-5955","issn":["1545-5955","1558-3783"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Automation Science and Engineering","raw_type":"journal-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086651818","display_name":"Fei Pan","orcid":"https://orcid.org/0000-0003-0550-4024"},"institutions":[{"id":"https://openalex.org/I168719708","display_name":"City University of Hong Kong","ror":"https://ror.org/03q8dnn23","country_code":"HK","type":"education","lineage":["https://openalex.org/I168719708"]}],"countries":["HK"],"is_corresponding":false,"raw_author_name":"Fei Pan","raw_affiliation_strings":["Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China","School of Interdisciplinary Studies, Lingnan University, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0003-0550-4024","affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China","institution_ids":["https://openalex.org/I168719708"]},{"raw_affiliation_string":"School of Interdisciplinary Studies, Lingnan University, Hong Kong, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023373913","display_name":"Shuxun Chen","orcid":"https://orcid.org/0000-0003-1113-2693"},"institutions":[{"id":"https://openalex.org/I168719708","display_name":"City University of Hong Kong","ror":"https://ror.org/03q8dnn23","country_code":"HK","type":"education","lineage":["https://openalex.org/I168719708"]}],"countries":["HK"],"is_corresponding":false,"raw_author_name":"Shuxun Chen","raw_affiliation_strings":["Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0003-1113-2693","affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China","institution_ids":["https://openalex.org/I168719708"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060677853","display_name":"Liushuai Zheng","orcid":"https://orcid.org/0000-0003-0890-5149"},"institutions":[{"id":"https://openalex.org/I168719708","display_name":"City University of Hong Kong","ror":"https://ror.org/03q8dnn23","country_code":"HK","type":"education","lineage":["https://openalex.org/I168719708"]}],"countries":["HK"],"is_corresponding":false,"raw_author_name":"Liushuai Zheng","raw_affiliation_strings":["Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0003-0890-5149","affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China","institution_ids":["https://openalex.org/I168719708"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073815536","display_name":"Shaohua Zhi","orcid":"https://orcid.org/0000-0003-3151-9713"},"institutions":[{"id":"https://openalex.org/I14243506","display_name":"Hong Kong Polytechnic University","ror":"https://ror.org/0030zas98","country_code":"HK","type":"education","lineage":["https://openalex.org/I14243506"]}],"countries":["HK"],"is_corresponding":false,"raw_author_name":"Shaohua Zhi","raw_affiliation_strings":["Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China","School of Interdisciplinary Studies, Lingnan University, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0003-3151-9713","affiliations":[{"raw_affiliation_string":"Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China","institution_ids":["https://openalex.org/I14243506"]},{"raw_affiliation_string":"School of Interdisciplinary Studies, Lingnan University, Hong Kong, China","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xi Chen","orcid":"https://orcid.org/0000-0002-8911-4172"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xi Chen","raw_affiliation_strings":["School of Interdisciplinary Studies, Lingnan University, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0002-8911-4172","affiliations":[{"raw_affiliation_string":"School of Interdisciplinary Studies, Lingnan University, Hong Kong, China","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100382905","display_name":"Dong Sun","orcid":"https://orcid.org/0000-0003-3945-4037"},"institutions":[{"id":"https://openalex.org/I168719708","display_name":"City University of Hong Kong","ror":"https://ror.org/03q8dnn23","country_code":"HK","type":"education","lineage":["https://openalex.org/I168719708"]}],"countries":["HK"],"is_corresponding":false,"raw_author_name":"Dong Sun","raw_affiliation_strings":["Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China"],"raw_orcid":"https://orcid.org/0000-0003-3945-4037","affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China","institution_ids":["https://openalex.org/I168719708"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.0382,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.92365782,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":98},"biblio":{"volume":"22","issue":null,"first_page":"11955","last_page":"11967"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9915000200271606,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9915000200271606,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9815000295639038,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9419999718666077,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pipette","display_name":"Pipette","score":0.6453229188919067},{"id":"https://openalex.org/keywords/calibration","display_name":"Calibration","score":0.6372896432876587},{"id":"https://openalex.org/keywords/microinjection","display_name":"Microinjection","score":0.6209605932235718},{"id":"https://openalex.org/keywords/somatic-cell","display_name":"Somatic cell","score":0.43279343843460083},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.41887587308883667},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.34292322397232056},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.2191496193408966},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.20349335670471191},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.17678803205490112},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.1332755982875824}],"concepts":[{"id":"https://openalex.org/C151552171","wikidata":"https://www.wikidata.org/wiki/Q163373","display_name":"Pipette","level":2,"score":0.6453229188919067},{"id":"https://openalex.org/C165838908","wikidata":"https://www.wikidata.org/wiki/Q736777","display_name":"Calibration","level":2,"score":0.6372896432876587},{"id":"https://openalex.org/C62525196","wikidata":"https://www.wikidata.org/wiki/Q3311945","display_name":"Microinjection","level":2,"score":0.6209605932235718},{"id":"https://openalex.org/C134305767","wikidata":"https://www.wikidata.org/wiki/Q725508","display_name":"Somatic cell","level":3,"score":0.43279343843460083},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.41887587308883667},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.34292322397232056},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.2191496193408966},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20349335670471191},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.17678803205490112},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1332755982875824},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/tase.2025.3533657","is_oa":false,"landing_page_url":"https://doi.org/10.1109/tase.2025.3533657","pdf_url":null,"source":{"id":"https://openalex.org/S34881539","display_name":"IEEE Transactions on Automation Science and Engineering","issn_l":"1545-5955","issn":["1545-5955","1558-3783"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Transactions on Automation Science and Engineering","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G1229344127","display_name":null,"funder_award_id":"11209917","funder_id":"https://openalex.org/F4320322887","funder_display_name":"The Research Council"},{"id":"https://openalex.org/G3887204670","display_name":"Robotic Cell Injection Surgery System for the Safe and Specific Genetic Modification in Single Cells","funder_award_id":"11209917","funder_id":"https://openalex.org/F4320321592","funder_display_name":"Research Grants Council, University Grants Committee"}],"funders":[{"id":"https://openalex.org/F4320321592","display_name":"Research Grants Council, University Grants Committee","ror":"https://ror.org/00djwmt25"},{"id":"https://openalex.org/F4320322887","display_name":"The Research Council","ror":"https://ror.org/03tcppy59"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1629139275","https://openalex.org/W1966709725","https://openalex.org/W1979450868","https://openalex.org/W1997637802","https://openalex.org/W2003742338","https://openalex.org/W2029245933","https://openalex.org/W2048811232","https://openalex.org/W2067744732","https://openalex.org/W2074175621","https://openalex.org/W2078631909","https://openalex.org/W2095232715","https://openalex.org/W2095614026","https://openalex.org/W2110767843","https://openalex.org/W2111912669","https://openalex.org/W2120481893","https://openalex.org/W2124535811","https://openalex.org/W2126703740","https://openalex.org/W2127347749","https://openalex.org/W2136507898","https://openalex.org/W2165767879","https://openalex.org/W2168976549","https://openalex.org/W2171192288","https://openalex.org/W2188472255","https://openalex.org/W2288384663","https://openalex.org/W2337970408","https://openalex.org/W2345393041","https://openalex.org/W2566715632","https://openalex.org/W2766159663","https://openalex.org/W2808732595","https://openalex.org/W2896481637","https://openalex.org/W2998948479","https://openalex.org/W3000565914","https://openalex.org/W3030834860","https://openalex.org/W4312251707","https://openalex.org/W4387789729","https://openalex.org/W4403371452"],"related_works":["https://openalex.org/W2381292741","https://openalex.org/W2116564729","https://openalex.org/W4386820634","https://openalex.org/W2984569590","https://openalex.org/W4245822815","https://openalex.org/W2208703520","https://openalex.org/W85024798","https://openalex.org/W2386443984","https://openalex.org/W3143969868","https://openalex.org/W2068582042"],"abstract_inverted_index":{"This":[0],"paper":[1,82,229,248],"proposes":[2,230],"a":[3,60,89,93,105,153,159,184,231],"practical":[4,232],"micropipette-image":[5],"2-D":[6,181],"pixel":[7,254],"and":[8,46,74,92,112,115,158,190,257,273,283,298],"3-D":[9,191],"spatial":[10,258],"coordination)":[11],"calibration":[12,218,299],"method":[13,26,86],"indispensable":[14],"to":[15,165,170,211,294],"somatic":[16,97,123,208],"cell":[17,129,209],"microinjection,":[18],"leveraging":[19],"advancements":[20],"in":[21,34,75,187,285],"modern":[22],"motorized":[23,221],"micromanipulators.":[24],"The":[25,99,117,247],"determines":[27],"the":[28,32,35,40,43,47,50,66,70,76,81,137,140,172,241,253,265,269,274],"depth":[29],"information":[30],"of":[31,49,156,162,174,183,199],"micropipette":[33,44,90,101,185],"microscope":[36,188],"field":[37],"by":[38,263],"assessing":[39],"contact":[41,58,239],"between":[42,179,252],"tip":[45,67,91,186,238,266],"bottom":[48,72,243,271],"culture":[51],"dish.":[52],"It":[53],"uses":[54],"recoverable":[55,235],"deformation":[56,236],"upon":[57,237],"as":[59],"criterion":[61],"for":[62,87,96,131,244],"precise":[63,245],"positioning,":[64],"ensuring":[65,264],"is":[68],"on":[69,146],"dish\u2019s":[71,270],"surface":[73,272],"microscope\u2019s":[77,275],"focus":[78,276],"plane.":[79,277],"Additionally,":[80],"introduces":[83],"an":[84,196,200],"on-the-spot":[85],"breaking":[88],"preprocessing":[94,118],"technique":[95,119],"cells.":[98],"proposed":[100],"tip-breaking":[102],"method,":[103],"using":[104],"low-cost":[106],"acrylic":[107],"ring,":[108],"overcomes":[109],"previous":[110],"drawbacks":[111],"proves":[113],"quick":[114],"user-friendly.":[116],"converts":[120],"fully":[121],"adherent":[122],"cells":[124],"into":[125],"semi-adherent":[126],"cells,":[127,151],"increasing":[128],"thickness":[130],"easier":[132],"puncturing.":[133],"Combining":[134],"these":[135],"techniques,":[136],"study":[138,167],"validated":[139],"approaches":[141],"through":[142],"over":[143],"900":[144],"injections":[145],"human":[147],"dermal":[148],"fibroblast":[149],"(HDF)":[150],"achieving":[152],"success":[154],"rate":[155,161],"53.3%":[157],"survival":[160],"95.8%.":[163],"Note":[164],"Practitioners\u2013This":[166],"was":[168],"initiated":[169],"address":[171],"challenge":[173],"establishing":[175],"accurate":[176],"mapping":[177],"relationships":[178,251],"observed":[180],"coordinates":[182],"vision":[189],"positional":[192],"data":[193],"obtained":[194],"from":[195],"external":[197],"controller":[198],"XYZ":[201],"micromanipulator.":[202],"However,":[203],"its":[204],"relevance":[205],"extends":[206],"beyond":[207],"microinjection":[210],"other":[212,286],"micromanipulation":[213,287],"applications":[214,288],"like":[215],"micro-assembly.":[216],"Common":[217],"methods":[219],"involve":[220],"objectives":[222],"or":[223],"stereo":[224],"microscopic":[225],"vision,":[226],"whereas":[227],"our":[228],"approach":[233],"utilizing":[234],"with":[240,268],"dish":[242],"positioning.":[246],"mathematically":[249],"characterizes":[250],"coordinate":[255,259],"system":[256,296],"systems,":[260],"demonstrating":[261],"problem-solving":[262],"aligns":[267],"Although":[278],"experiments":[279],"affirm":[280],"feasibility,":[281],"refinement":[282],"testing":[284],"are":[289],"pending.":[290],"Future":[291],"research":[292],"aims":[293],"enhance":[295],"modeling":[297],"accuracy.":[300]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2}],"updated_date":"2025-12-27T23:08:20.325037","created_date":"2025-10-10T00:00:00"}