{"id":"https://openalex.org/W4311413449","doi":"https://doi.org/10.1109/sensors52175.2022.9967202","title":"Low-cost colorimetric alternative of qPCR for DNA sensing based on intercalation with methylene blue","display_name":"Low-cost colorimetric alternative of qPCR for DNA sensing based on intercalation with methylene blue","publication_year":2022,"publication_date":"2022-10-30","ids":{"openalex":"https://openalex.org/W4311413449","doi":"https://doi.org/10.1109/sensors52175.2022.9967202"},"language":"en","primary_location":{"id":"doi:10.1109/sensors52175.2022.9967202","is_oa":false,"landing_page_url":"https://doi.org/10.1109/sensors52175.2022.9967202","pdf_url":null,"source":{"id":"https://openalex.org/S4363604906","display_name":"2022 IEEE Sensors","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2022 IEEE Sensors","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033878688","display_name":"Ruchira Nandeshwar","orcid":"https://orcid.org/0000-0003-1119-1036"},"institutions":[{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]},{"id":"https://openalex.org/I4210135821","display_name":"Bombay Hospital","ror":"https://ror.org/03xmsh521","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210135821"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Ruchira Nandeshwar","raw_affiliation_strings":["IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","institution_ids":["https://openalex.org/I162827531","https://openalex.org/I4210135821"]},{"raw_affiliation_string":"Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014476494","display_name":"Avani Kulkarni","orcid":null},"institutions":[{"id":"https://openalex.org/I4210135821","display_name":"Bombay Hospital","ror":"https://ror.org/03xmsh521","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210135821"]},{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Avani Kulkarni","raw_affiliation_strings":["IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","institution_ids":["https://openalex.org/I162827531","https://openalex.org/I4210135821"]},{"raw_affiliation_string":"Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033740806","display_name":"Shruti Ahuja","orcid":null},"institutions":[{"id":"https://openalex.org/I4210135821","display_name":"Bombay Hospital","ror":"https://ror.org/03xmsh521","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210135821"]},{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Shruti Ahuja","raw_affiliation_strings":["IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India","Centre for Research in Nanotechnology and Science (CRNTS), IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","institution_ids":["https://openalex.org/I162827531","https://openalex.org/I4210135821"]},{"raw_affiliation_string":"Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]},{"raw_affiliation_string":"Centre for Research in Nanotechnology and Science (CRNTS), IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102002845","display_name":"Manish Kumar","orcid":"https://orcid.org/0000-0001-8035-3399"},"institutions":[{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"M. S. Kumar","raw_affiliation_strings":["IIT Bombay,Department of Biosciences and Bioengineering (BSBE),Mumbai,India","Department of Biosciences and Bioengineering (BSBE), IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,Department of Biosciences and Bioengineering (BSBE),Mumbai,India","institution_ids":["https://openalex.org/I162827531"]},{"raw_affiliation_string":"Department of Biosciences and Bioengineering (BSBE), IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009556533","display_name":"Kiran Kondabagil","orcid":"https://orcid.org/0000-0002-7942-023X"},"institutions":[{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Kiran Kondabagil","raw_affiliation_strings":["IIT Bombay,Department of Biosciences and Bioengineering (BSBE),Mumbai,India","Department of Biosciences and Bioengineering (BSBE), IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,Department of Biosciences and Bioengineering (BSBE),Mumbai,India","institution_ids":["https://openalex.org/I162827531"]},{"raw_affiliation_string":"Department of Biosciences and Bioengineering (BSBE), IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072493084","display_name":"Siddharth Tallur","orcid":"https://orcid.org/0000-0003-1399-2187"},"institutions":[{"id":"https://openalex.org/I162827531","display_name":"Indian Institute of Technology Bombay","ror":"https://ror.org/02qyf5152","country_code":"IN","type":"education","lineage":["https://openalex.org/I162827531"]},{"id":"https://openalex.org/I4210135821","display_name":"Bombay Hospital","ror":"https://ror.org/03xmsh521","country_code":"IN","type":"healthcare","lineage":["https://openalex.org/I4210135821"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Siddharth Tallur","raw_affiliation_strings":["IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India"],"affiliations":[{"raw_affiliation_string":"IIT Bombay,AIMS Lab,Department of Electrical Engineering (EE),Mumbai,India","institution_ids":["https://openalex.org/I162827531","https://openalex.org/I4210135821"]},{"raw_affiliation_string":"Department of Electrical Engineering (EE), AIMS Lab, IIT Bombay, Mumbai, India","institution_ids":["https://openalex.org/I162827531"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5033878688"],"corresponding_institution_ids":["https://openalex.org/I162827531","https://openalex.org/I4210135821"],"apc_list":null,"apc_paid":null,"fwci":0.3206,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.49788775,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"4"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.9987000226974487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11472","display_name":"Analytical Chemistry and Sensors","score":0.9965000152587891,"subfield":{"id":"https://openalex.org/subfields/1502","display_name":"Bioengineering"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intercalation","display_name":"Intercalation (chemistry)","score":0.9082223176956177},{"id":"https://openalex.org/keywords/absorbance","display_name":"Absorbance","score":0.898926317691803},{"id":"https://openalex.org/keywords/methylene-blue","display_name":"Methylene blue","score":0.8649333715438843},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.6190915107727051},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.5366358757019043},{"id":"https://openalex.org/keywords/linear-range","display_name":"Linear range","score":0.45532846450805664},{"id":"https://openalex.org/keywords/detection-limit","display_name":"Detection limit","score":0.3991120457649231},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.3916131556034088},{"id":"https://openalex.org/keywords/analytical-chemistry","display_name":"Analytical Chemistry (journal)","score":0.3849455714225769},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.214124858379364},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.11355242133140564},{"id":"https://openalex.org/keywords/inorganic-chemistry","display_name":"Inorganic chemistry","score":0.09275004267692566}],"concepts":[{"id":"https://openalex.org/C137824038","wikidata":"https://www.wikidata.org/wiki/Q175562","display_name":"Intercalation (chemistry)","level":2,"score":0.9082223176956177},{"id":"https://openalex.org/C98015330","wikidata":"https://www.wikidata.org/wiki/Q907315","display_name":"Absorbance","level":2,"score":0.898926317691803},{"id":"https://openalex.org/C2779191827","wikidata":"https://www.wikidata.org/wiki/Q422134","display_name":"Methylene blue","level":4,"score":0.8649333715438843},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6190915107727051},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5366358757019043},{"id":"https://openalex.org/C10349863","wikidata":"https://www.wikidata.org/wiki/Q6553497","display_name":"Linear range","level":3,"score":0.45532846450805664},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.3991120457649231},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3916131556034088},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.3849455714225769},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.214124858379364},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11355242133140564},{"id":"https://openalex.org/C179104552","wikidata":"https://www.wikidata.org/wiki/Q11165","display_name":"Inorganic chemistry","level":1,"score":0.09275004267692566},{"id":"https://openalex.org/C65165184","wikidata":"https://www.wikidata.org/wiki/Q218831","display_name":"Photocatalysis","level":3,"score":0.0},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/sensors52175.2022.9967202","is_oa":false,"landing_page_url":"https://doi.org/10.1109/sensors52175.2022.9967202","pdf_url":null,"source":{"id":"https://openalex.org/S4363604906","display_name":"2022 IEEE Sensors","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2022 IEEE Sensors","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W2023786370","https://openalex.org/W2033105713","https://openalex.org/W2084353434","https://openalex.org/W2125164011","https://openalex.org/W2133240637","https://openalex.org/W2155221828","https://openalex.org/W2322786785","https://openalex.org/W2340376277","https://openalex.org/W2529061178","https://openalex.org/W2907937002","https://openalex.org/W3081876595","https://openalex.org/W3108611440","https://openalex.org/W3115514697","https://openalex.org/W3164662379","https://openalex.org/W3214442426","https://openalex.org/W4205694116","https://openalex.org/W4281487095","https://openalex.org/W4389174769","https://openalex.org/W6859640787"],"related_works":["https://openalex.org/W2319334673","https://openalex.org/W4382053572","https://openalex.org/W2116763612","https://openalex.org/W2966296742","https://openalex.org/W4362452082","https://openalex.org/W2367517815","https://openalex.org/W1986805515","https://openalex.org/W2050356512","https://openalex.org/W2383409080","https://openalex.org/W2357046628"],"abstract_inverted_index":{"When":[0],"methylene":[1],"blue":[2],"(MB)":[3],"dye":[4],"is":[5,17],"added":[6],"to":[7,20,41,65,102],"a":[8,13,34,43,59,72,99],"test":[9],"sample":[10],"containing":[11],"DNA,":[12],"change":[14,51,83],"in":[15,52,84,92],"absorbance":[16],"observed":[18],"due":[19],"intercalation":[21],"of":[22,74,88,90],"MB":[23],"into":[24],"the":[25,50],"DNA":[26,68],"base":[27],"pairs.":[28],"In":[29],"this":[30],"work,":[31],"we":[32],"demonstrate":[33],"low-cost":[35,100],"phase":[36],"sensitive":[37,45],"detection":[38],"(PSD)":[39],"system":[40],"realize":[42],"highly":[44],"colorimetric":[46],"sensor":[47,57,79],"for":[48,62],"detecting":[49],"absorbance.":[53],"The":[54,78],"optical":[55],"PSD":[56],"produces":[58,81],"linear":[60],"response":[61],"1":[63],"ng/\u00b5L":[64,67],"15":[66],"concentration":[69],"range":[70],"with":[71,86],"sensitivity":[73],"approximately":[75],"20":[76],"mV/ng/\u00b5L.":[77],"also":[80],"measurable":[82],"output":[85],"progression":[87],"number":[89],"cycles":[91],"PCR,":[93],"and":[94],"could":[95],"be":[96],"used":[97],"as":[98],"alternative":[101],"quantitative":[103],"PCR":[104],"(qPCR).":[105]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}
