{"id":"https://openalex.org/W2045738078","doi":"https://doi.org/10.1109/nems.2013.6559882","title":"Nanofocused electric field for localized single cell nanoelectroporation with membrane reversibility","display_name":"Nanofocused electric field for localized single cell nanoelectroporation with membrane reversibility","publication_year":2013,"publication_date":"2013-04-01","ids":{"openalex":"https://openalex.org/W2045738078","doi":"https://doi.org/10.1109/nems.2013.6559882","mag":"2045738078"},"language":"en","primary_location":{"id":"doi:10.1109/nems.2013.6559882","is_oa":false,"landing_page_url":"https://doi.org/10.1109/nems.2013.6559882","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084793977","display_name":"Tuhin Subhra Santra","orcid":"https://orcid.org/0000-0002-9403-2155"},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":true,"raw_author_name":"Tuhin Subhra Santra","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004144189","display_name":"Chih\u2010Yi Chiu","orcid":"https://orcid.org/0000-0002-2859-6120"},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"C. Chiu","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003565868","display_name":"Nishant Agarwal","orcid":"https://orcid.org/0000-0001-9290-4602"},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Nishant Agarwal","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046041613","display_name":"Aswin Ganatathi","orcid":null},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Aswin Ganatathi","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062278211","display_name":"Pen\u2010Cheng Wang","orcid":"https://orcid.org/0000-0003-1541-6466"},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Pen-Cheng Wang","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059907602","display_name":"Fan\u2010Gang Tseng","orcid":"https://orcid.org/0000-0001-7654-6905"},"institutions":[{"id":"https://openalex.org/I25846049","display_name":"National Tsing Hua University","ror":"https://ror.org/00zdnkx70","country_code":"TW","type":"education","lineage":["https://openalex.org/I25846049"]},{"id":"https://openalex.org/I84653119","display_name":"Academia Sinica","ror":"https://ror.org/05bxb3784","country_code":"TW","type":"facility","lineage":["https://openalex.org/I84653119"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Fan-Gang Tseng","raw_affiliation_strings":["Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan"],"affiliations":[{"raw_affiliation_string":"Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taiwan","institution_ids":["https://openalex.org/I84653119"]},{"raw_affiliation_string":"Institute of Nano Engineering and Microsystems, Department of Engineering and System Science, National Tsing Hua University, Taiwan","institution_ids":["https://openalex.org/I25846049"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5084793977"],"corresponding_institution_ids":["https://openalex.org/I25846049","https://openalex.org/I84653119"],"apc_list":null,"apc_paid":null,"fwci":0.346,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.67572259,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":null,"issue":null,"first_page":"961","last_page":"964"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.9897000193595886,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9728999733924866,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/electroporation","display_name":"Electroporation","score":0.9074838757514954},{"id":"https://openalex.org/keywords/electric-field","display_name":"Electric field","score":0.6311610341072083},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.5649645328521729},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.5643357634544373},{"id":"https://openalex.org/keywords/biomolecule","display_name":"Biomolecule","score":0.5455299615859985},{"id":"https://openalex.org/keywords/cell-membrane","display_name":"Cell membrane","score":0.544411838054657},{"id":"https://openalex.org/keywords/membrane","display_name":"Membrane","score":0.5182997584342957},{"id":"https://openalex.org/keywords/viability-assay","display_name":"Viability assay","score":0.508953332901001},{"id":"https://openalex.org/keywords/hela","display_name":"HeLa","score":0.4880870580673218},{"id":"https://openalex.org/keywords/electrode","display_name":"Electrode","score":0.4863479733467102},{"id":"https://openalex.org/keywords/biophysics","display_name":"Biophysics","score":0.4857047498226166},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.48263248801231384},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.30232831835746765},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.14887729287147522},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.07214313745498657}],"concepts":[{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.9074838757514954},{"id":"https://openalex.org/C60799052","wikidata":"https://www.wikidata.org/wiki/Q46221","display_name":"Electric field","level":2,"score":0.6311610341072083},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5649645328521729},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5643357634544373},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.5455299615859985},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.544411838054657},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.5182997584342957},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.508953332901001},{"id":"https://openalex.org/C2777366897","wikidata":"https://www.wikidata.org/wiki/Q847482","display_name":"HeLa","level":3,"score":0.4880870580673218},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.4863479733467102},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4857047498226166},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.48263248801231384},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.30232831835746765},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14887729287147522},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.07214313745498657},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/nems.2013.6559882","is_oa":false,"landing_page_url":"https://doi.org/10.1109/nems.2013.6559882","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321040","display_name":"National Science Council","ror":"https://ror.org/02kv4zf79"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1490324405","https://openalex.org/W1965469172","https://openalex.org/W1970047913","https://openalex.org/W1980354101","https://openalex.org/W1980895206","https://openalex.org/W1985040895","https://openalex.org/W2006772808","https://openalex.org/W2051658955","https://openalex.org/W2065723545","https://openalex.org/W2074039393","https://openalex.org/W2075279905","https://openalex.org/W2082781273","https://openalex.org/W2166434586","https://openalex.org/W2407854904"],"related_works":["https://openalex.org/W2085776530","https://openalex.org/W2785437298","https://openalex.org/W2516326828","https://openalex.org/W2029447323","https://openalex.org/W4245893494","https://openalex.org/W2324662686","https://openalex.org/W2072032726","https://openalex.org/W2063820044","https://openalex.org/W99661837","https://openalex.org/W2979267483"],"abstract_inverted_index":{"Despite":[0],"the":[1,12,18,31,35,69,73],"significant":[2],"research":[3],"in":[4,16,105],"electroporation,":[5],"high":[6,39,43,126,150,155],"electric":[7,78,97],"field":[8,98],"was":[9],"applied":[10],"to":[11,99,183],"whole":[13],"cells":[14,23],"resulted":[15],"permeabilizing":[17],"membrane":[19,37,62,120],"of":[20,22,34,68,75,146,168,181],"millions":[21],"without":[24],"reversibility":[25],"[1].":[26],"To":[27],"deliver":[28,184],"biomolecules":[29,101,141],"through":[30,142],"specific":[32,66,144],"region":[33,67,134,145],"cell":[36,40,61,71,104,119,127,148,156,169],"with":[38,72,83,125,149],"viability":[41,128],"and":[42,51,86,121,130,154],"transfection":[44,151],"rate":[45,152],"is":[46],"important":[47],"for":[48],"various":[49],"biological":[50],"therapeutic":[52],"applications.This":[53],"report":[54],"presents":[55],"a":[56,143,175],"new":[57],"type":[58],"localized":[59],"single":[60,70,147,186],"electroporation":[63,124,133],"(LSCMEP),":[64],"at":[65],"application":[74],"800":[76],"\u03bcs":[77],"pulse.":[79],"The":[80],"ITO":[81],"nano-electrodes":[82,113],"100nm":[84],"thickness":[85],"500":[87],"nm":[88],"gap":[89,110],"between":[90,111],"two":[91,112],"electrodes":[92],"can":[93,114],"generate":[94],"an":[95],"intense":[96],"track":[100],"inside":[102,185],"HeLa":[103],"our":[106],"studies.":[107],"This":[108,158],"small":[109],"neglect":[115],"thermal":[116],"effect":[117],"on":[118],"permit":[122],"reversible":[123],"(90%)":[129],"minimum":[131],"effected":[132],"(0.48":[135],"\u03bcm).":[136],"Our":[137],"approach":[138],"successfully":[139],"delivers":[140],"(82%)":[153],"viability.":[157],"process,":[159],"not":[160],"only":[161],"generates":[162],"well-controlled":[163],"nano-pores":[164],"allowing":[165],"rapid":[166],"recovery":[167],"membrane,":[170],"but":[171],"also":[172],"it":[173],"provides":[174],"clear":[176],"optical":[177],"path":[178],"potentially":[179],"tracking":[180],"drugs":[182],"cell.":[187]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-03-25T13:04:00.132906","created_date":"2025-10-10T00:00:00"}