{"id":"https://openalex.org/W2120643414","doi":"https://doi.org/10.1109/nems.2009.5068572","title":"Development of a novel perfusion microfluidic cell culture device for cell-based assays","display_name":"Development of a novel perfusion microfluidic cell culture device for cell-based assays","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2120643414","doi":"https://doi.org/10.1109/nems.2009.5068572","mag":"2120643414"},"language":"en","primary_location":{"id":"doi:10.1109/nems.2009.5068572","is_oa":false,"landing_page_url":"https://doi.org/10.1109/nems.2009.5068572","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032574555","display_name":"Yunhuan Zheng","orcid":null},"institutions":[{"id":"https://openalex.org/I4210147322","display_name":"Shanghai Institute of Microsystem and Information Technology","ror":"https://ror.org/04nytyj38","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210147322"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yunhuan Zheng","raw_affiliation_strings":["Graduate School, Chinese Academy and Sciences, China","Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China"],"affiliations":[{"raw_affiliation_string":"Graduate School, Chinese Academy and Sciences, China","institution_ids":[]},{"raw_affiliation_string":"Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China","institution_ids":["https://openalex.org/I4210147322"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017441654","display_name":"Jianzhang Wu","orcid":"https://orcid.org/0000-0002-2782-745X"},"institutions":[{"id":"https://openalex.org/I4210147322","display_name":"Shanghai Institute of Microsystem and Information Technology","ror":"https://ror.org/04nytyj38","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210147322"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianzhang Wu","raw_affiliation_strings":["Graduate School, Chinese Academy and Sciences, China","Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China"],"affiliations":[{"raw_affiliation_string":"Graduate School, Chinese Academy and Sciences, China","institution_ids":[]},{"raw_affiliation_string":"Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China","institution_ids":["https://openalex.org/I4210147322"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005481589","display_name":"Jianbo Shao","orcid":"https://orcid.org/0000-0002-4052-0612"},"institutions":[{"id":"https://openalex.org/I4210147322","display_name":"Shanghai Institute of Microsystem and Information Technology","ror":"https://ror.org/04nytyj38","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210147322"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianbo Shao","raw_affiliation_strings":["Graduate School, Chinese Academy and Sciences, China","Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China"],"affiliations":[{"raw_affiliation_string":"Graduate School, Chinese Academy and Sciences, China","institution_ids":[]},{"raw_affiliation_string":"Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China","institution_ids":["https://openalex.org/I4210147322"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074177874","display_name":"Qinghui Jin","orcid":"https://orcid.org/0000-0003-2591-037X"},"institutions":[{"id":"https://openalex.org/I4210147322","display_name":"Shanghai Institute of Microsystem and Information Technology","ror":"https://ror.org/04nytyj38","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210147322"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qinghui Jin","raw_affiliation_strings":["Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China"],"affiliations":[{"raw_affiliation_string":"Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China","institution_ids":["https://openalex.org/I4210147322"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100636906","display_name":"Jianlong Zhao","orcid":"https://orcid.org/0000-0002-8636-204X"},"institutions":[{"id":"https://openalex.org/I4210147322","display_name":"Shanghai Institute of Microsystem and Information Technology","ror":"https://ror.org/04nytyj38","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210147322"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianlong Zhao","raw_affiliation_strings":["Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China"],"affiliations":[{"raw_affiliation_string":"Nanotechnology Laboratory, Shanghai Institute of Micro System and Information Technology, China","institution_ids":["https://openalex.org/I4210147322"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5032574555"],"corresponding_institution_ids":["https://openalex.org/I4210147322"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.16654135,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":null,"first_page":"257","last_page":"260"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microfluidics","display_name":"Microfluidics","score":0.803917646408081},{"id":"https://openalex.org/keywords/cell-culture","display_name":"Cell culture","score":0.6956315040588379},{"id":"https://openalex.org/keywords/concentration-gradient","display_name":"Concentration gradient","score":0.6083595752716064},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.5805640816688538},{"id":"https://openalex.org/keywords/biomedical-engineering","display_name":"Biomedical engineering","score":0.5733261704444885},{"id":"https://openalex.org/keywords/microfluidic-chip","display_name":"Microfluidic chip","score":0.5641969442367554},{"id":"https://openalex.org/keywords/perfusion","display_name":"Perfusion","score":0.5422995090484619},{"id":"https://openalex.org/keywords/organ-on-a-chip","display_name":"Organ-on-a-chip","score":0.5119439363479614},{"id":"https://openalex.org/keywords/tissue-engineering","display_name":"Tissue engineering","score":0.4682629704475403},{"id":"https://openalex.org/keywords/chip","display_name":"Chip","score":0.42310231924057007},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.41653817892074585},{"id":"https://openalex.org/keywords/drug","display_name":"Drug","score":0.4105340242385864},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.40357857942581177},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.34392574429512024},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.32923629879951477},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.1536586582660675},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.13893038034439087},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.1277303695678711},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.12040922045707703},{"id":"https://openalex.org/keywords/pharmacology","display_name":"Pharmacology","score":0.10612419247627258},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.08225101232528687},{"id":"https://openalex.org/keywords/electrical-engineering","display_name":"Electrical engineering","score":0.07944506406784058},{"id":"https://openalex.org/keywords/internal-medicine","display_name":"Internal medicine","score":0.07530513405799866}],"concepts":[{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.803917646408081},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.6956315040588379},{"id":"https://openalex.org/C3018453882","wikidata":"https://www.wikidata.org/wiki/Q17104113","display_name":"Concentration gradient","level":2,"score":0.6083595752716064},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5805640816688538},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.5733261704444885},{"id":"https://openalex.org/C2988198817","wikidata":"https://www.wikidata.org/wiki/Q633261","display_name":"Microfluidic chip","level":3,"score":0.5641969442367554},{"id":"https://openalex.org/C146957229","wikidata":"https://www.wikidata.org/wiki/Q1266915","display_name":"Perfusion","level":2,"score":0.5422995090484619},{"id":"https://openalex.org/C165932988","wikidata":"https://www.wikidata.org/wiki/Q7101746","display_name":"Organ-on-a-chip","level":3,"score":0.5119439363479614},{"id":"https://openalex.org/C49892992","wikidata":"https://www.wikidata.org/wiki/Q1540285","display_name":"Tissue engineering","level":2,"score":0.4682629704475403},{"id":"https://openalex.org/C165005293","wikidata":"https://www.wikidata.org/wiki/Q1074500","display_name":"Chip","level":2,"score":0.42310231924057007},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.41653817892074585},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.4105340242385864},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40357857942581177},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34392574429512024},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.32923629879951477},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1536586582660675},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.13893038034439087},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.1277303695678711},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.12040922045707703},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.10612419247627258},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08225101232528687},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.07944506406784058},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.07530513405799866},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/nems.2009.5068572","is_oa":false,"landing_page_url":"https://doi.org/10.1109/nems.2009.5068572","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.699999988079071,"display_name":"Good health and well-being"}],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W1969435013","https://openalex.org/W2013117405","https://openalex.org/W2019239417","https://openalex.org/W2024971068","https://openalex.org/W2033117797","https://openalex.org/W2045923003","https://openalex.org/W2145759350"],"related_works":["https://openalex.org/W4244444919","https://openalex.org/W1979921047","https://openalex.org/W2058246061","https://openalex.org/W2356576053","https://openalex.org/W2060742235","https://openalex.org/W2912139356","https://openalex.org/W2103956795","https://openalex.org/W4367182701","https://openalex.org/W4200532018","https://openalex.org/W2741970576"],"abstract_inverted_index":{"This":[0],"study":[1],"reports":[2],"on":[3,23,118],"the":[4,54,57,79,83,92,97,112,115,122,151,154,160],"development":[5],"of":[6,31,34,56,91,99,105,114,129,135,169],"a":[7,24,39,60,127],"novel":[8],"perfusion":[9,148],"microfluidic":[10,155],"cell":[11,20,36,156],"culture":[12,37,80,157],"device":[13,29,158],"integrated":[14],"drug":[15,40,75,172],"delivering,":[16],"fluid":[17,93,119],"controlling":[18],"and":[19,43,51,121,140,176],"culturing":[21,149],"functions":[22],"two-layer":[25],"PDMS":[26],"chip.":[27,152],"The":[28],"consists":[30],"an":[32],"array":[33],"6\u00d76":[35],"chambers,":[38],"gradient":[41,67,74,131],"generator":[42],"two":[44],"control":[45,90],"valves.":[46],"Cells":[47],"are":[48,142],"physically":[49],"trapped":[50],"cultured":[52],"in":[53,150,166],"center":[55],"chamber":[58],"by":[59],"C-shaped":[61],"dam":[62],"when":[63],"cells":[64,106,137],"loading.":[65],"Drug":[66],"network":[68],"is":[69],"able":[70],"to":[71,77,95,125,162],"generate":[72,126],"6":[73],"concentrations":[76],"treat":[78],"cells.":[81],"And":[82],"hydraulic":[84,116],"valves":[85,117],"can":[86],"not":[87],"only":[88],"make":[89,163],"flow":[94],"mimic":[96],"microenvironment":[98],"cells,":[100],"but":[101],"keep":[102],"different":[103],"kind":[104],"separately":[107],"cultured.":[108],"We":[109],"have":[110],"investigated":[111],"effect":[113],"control,":[120],"chip's":[123],"ability":[124],"range":[128],"chemical":[130],"concentrations.":[132],"Three":[133],"kinds":[134],"Human":[136],"(L-02,":[138],"SMMC-7721":[139],"HUVEC-2C)":[141],"grown":[143],"well":[144],"after":[145],"three-day":[146],"continuous":[147],"Therefore":[153],"shows":[159],"potential":[161],"significant":[164],"impacts":[165],"cell-based":[167],"research":[168],"high":[170],"throughput":[171],"screening,":[173],"tissue":[174],"engineering":[175],"clinical":[177],"medicine.":[178]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}