{"id":"https://openalex.org/W2306198345","doi":"https://doi.org/10.1109/mhs.2015.7438268","title":"A method to reorganize the bone marrow-like tissue with suspension of bone marrow cells","display_name":"A method to reorganize the bone marrow-like tissue with suspension of bone marrow cells","publication_year":2015,"publication_date":"2015-11-01","ids":{"openalex":"https://openalex.org/W2306198345","doi":"https://doi.org/10.1109/mhs.2015.7438268","mag":"2306198345"},"language":"en","primary_location":{"id":"doi:10.1109/mhs.2015.7438268","is_oa":false,"landing_page_url":"https://doi.org/10.1109/mhs.2015.7438268","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012490423","display_name":"Kanae Sayo","orcid":null},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Kanae Sayo","raw_affiliation_strings":["Yokohama city University, Kanazawa-ku, Yokohama"],"affiliations":[{"raw_affiliation_string":"Yokohama city University, Kanazawa-ku, Yokohama","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065318724","display_name":"Shigehisa Aoki","orcid":"https://orcid.org/0000-0002-1778-8944"},"institutions":[{"id":"https://openalex.org/I177136067","display_name":"Saga University","ror":"https://ror.org/04f4wg107","country_code":"JP","type":"education","lineage":["https://openalex.org/I177136067"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigehisa Aoki","raw_affiliation_strings":["Saga University, Saga, Japan"],"affiliations":[{"raw_affiliation_string":"Saga University, Saga, Japan","institution_ids":["https://openalex.org/I177136067"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003500065","display_name":"Nobuhiko Kojima","orcid":"https://orcid.org/0000-0003-2555-0570"},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Nobuhiko Kojima","raw_affiliation_strings":["Yokohama city University, Kanazawa-ku, Yokohama"],"affiliations":[{"raw_affiliation_string":"Yokohama city University, Kanazawa-ku, Yokohama","institution_ids":["https://openalex.org/I89630735"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5012490423"],"corresponding_institution_ids":["https://openalex.org/I89630735"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.14334852,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"3"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":0.9959999918937683,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":0.9959999918937683,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9894999861717224,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9866999983787537,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bone-marrow","display_name":"Bone marrow","score":0.7869126796722412},{"id":"https://openalex.org/keywords/haematopoiesis","display_name":"Haematopoiesis","score":0.5783339738845825},{"id":"https://openalex.org/keywords/staining","display_name":"Staining","score":0.5683751106262207},{"id":"https://openalex.org/keywords/reticular-connective-tissue","display_name":"Reticular connective tissue","score":0.528399646282196},{"id":"https://openalex.org/keywords/pathology","display_name":"Pathology","score":0.46027207374572754},{"id":"https://openalex.org/keywords/mesenchymal-stem-cell","display_name":"Mesenchymal stem cell","score":0.4602465033531189},{"id":"https://openalex.org/keywords/bone-tissue","display_name":"Bone tissue","score":0.44698524475097656},{"id":"https://openalex.org/keywords/h-e-stain","display_name":"H&E stain","score":0.4420841336250305},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.4011037051677704},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.37508952617645264},{"id":"https://openalex.org/keywords/stem-cell","display_name":"Stem cell","score":0.35962897539138794},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.22443881630897522},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.16701409220695496}],"concepts":[{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.7869126796722412},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.5783339738845825},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.5683751106262207},{"id":"https://openalex.org/C95190701","wikidata":"https://www.wikidata.org/wiki/Q3268002","display_name":"Reticular connective tissue","level":2,"score":0.528399646282196},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.46027207374572754},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.4602465033531189},{"id":"https://openalex.org/C2778606738","wikidata":"https://www.wikidata.org/wiki/Q265868","display_name":"Bone tissue","level":2,"score":0.44698524475097656},{"id":"https://openalex.org/C125473707","wikidata":"https://www.wikidata.org/wiki/Q9914","display_name":"H&E stain","level":3,"score":0.4420841336250305},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4011037051677704},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.37508952617645264},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.35962897539138794},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22443881630897522},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.16701409220695496}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/mhs.2015.7438268","is_oa":false,"landing_page_url":"https://doi.org/10.1109/mhs.2015.7438268","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W2043548457"],"related_works":["https://openalex.org/W3163749585","https://openalex.org/W4399790878","https://openalex.org/W3080438050","https://openalex.org/W4394857391","https://openalex.org/W4394840445","https://openalex.org/W2063962692","https://openalex.org/W2044206368","https://openalex.org/W2392756543","https://openalex.org/W3216304216","https://openalex.org/W4246628858"],"abstract_inverted_index":{"To":[0],"elucidate":[1],"the":[2,11,27,39,44,60,95,101,124,130,145,151,157,177,183,192],"mechanism":[3],"of":[4,13,26,54,73,91,94],"hematopoiesis":[5],"and":[6,109],"bone":[7],"marrow":[8],"(BM)":[9],"diseases,":[10],"realization":[12],"BM":[14,28,41,65,102,159],"three-dimensional":[15],"(3D)":[16],"culture":[17],"system":[18],"is":[19,35,57],"very":[20],"important.":[21],"Because":[22],"more":[23],"than":[24],"90%":[25],"cells":[29,42,66,132,173],"are":[30],"non-adherent":[31],"blood":[32,131],"cells,":[33,103,166,171],"it":[34],"difficult":[36],"to":[37,58,100,190],"organize":[38],"dispersed-state":[40,64],"with":[43,140],"conventional":[45],"aggregation":[46,87],"methods":[47],"e.g.":[48],"hanging":[49],"drop":[50],"method.":[51],"The":[52],"objective":[53],"this":[55],"study":[56],"reproduce":[59],"BM-like":[61,116,146,178,193],"tissue":[62,117,147],"from":[63,120],"in":[67,106,144,156,176],"vitro.":[68],"We":[69],"reported":[70],"a":[71,85],"method":[72,82,98,184],"forming":[74],"aggregates":[75],"using":[76,185],"methylcellulose":[77],"(MC)":[78],"medium":[79,122,187],"[1].":[80],"This":[81,97],"can":[83],"make":[84],"3D":[86],"state":[88],"rapidly":[89],"regardless":[90],"adhesive":[92],"property":[93],"cells.":[96],"applied":[99],"they":[104],"gathered":[105],"10":[107],"minutes":[108],"formed":[110],"tissue-like":[111],"spheroids":[112],"within":[113],"24h.":[114],"Formed":[115],"could":[118],"remove":[119],"MC":[121,186],"on":[123],"Day":[125],"1.":[126],"By":[127,138],"hematoxylin-eosin":[128],"staining,":[129],"were":[133,148,174],"maintained":[134,175],"for":[135,167],"3":[136],"days.":[137],"staining":[139],"anti-CD68":[141],"antibody,":[142],"macrophages":[143],"found":[149],"at":[150],"same":[152],"frequency":[153],"as":[154],"those":[155],"intact":[158],"tissue.":[160,194],"It":[161],"was":[162,188],"confirmed":[163],"that":[164],"mesenchymal":[165],"example":[168],"CXCL12+":[169],"reticular":[170],"PDGFR\u03b1+":[172],"tissues.":[179],"From":[180],"these":[181],"results,":[182],"suitable":[189],"reconstitute":[191]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}