{"id":"https://openalex.org/W2053058572","doi":"https://doi.org/10.1109/mhs.2014.7006151","title":"Efficient generation of mature hepatocyte-like cells from human iPSCs","display_name":"Efficient generation of mature hepatocyte-like cells from human iPSCs","publication_year":2014,"publication_date":"2014-11-01","ids":{"openalex":"https://openalex.org/W2053058572","doi":"https://doi.org/10.1109/mhs.2014.7006151","mag":"2053058572"},"language":"en","primary_location":{"id":"doi:10.1109/mhs.2014.7006151","is_oa":false,"landing_page_url":"https://doi.org/10.1109/mhs.2014.7006151","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001413495","display_name":"Hinatsu Ohgane","orcid":null},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hinatsu Ohgane","raw_affiliation_strings":["Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","institution_ids":["https://openalex.org/I89630735"]},{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034580309","display_name":"Takanori Takebe","orcid":"https://orcid.org/0000-0002-6989-3041"},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takanori Takebe","raw_affiliation_strings":["Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","institution_ids":["https://openalex.org/I89630735"]},{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020626473","display_name":"Keisuke Sekine","orcid":"https://orcid.org/0000-0001-5133-0876"},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Keisuke Sekine","raw_affiliation_strings":["Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","institution_ids":["https://openalex.org/I89630735"]},{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103041481","display_name":"Hideki Taniguchi","orcid":"https://orcid.org/0000-0002-6186-9266"},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hideki Taniguchi","raw_affiliation_strings":["Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, Kanazawa-ku Yokohama, Japan","institution_ids":["https://openalex.org/I89630735"]},{"raw_affiliation_string":"Department of Regenerative Medicine, Yokohama City University, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan","institution_ids":["https://openalex.org/I89630735"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5001413495"],"corresponding_institution_ids":["https://openalex.org/I89630735"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0868316,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"2"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.9886000156402588,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11190","display_name":"3D Printing in Biomedical Research","score":0.9740999937057495,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/induced-pluripotent-stem-cell","display_name":"Induced pluripotent stem cell","score":0.8482058048248291},{"id":"https://openalex.org/keywords/hepatocyte","display_name":"Hepatocyte","score":0.7926762104034424},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.6812251806259155},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.5663834810256958},{"id":"https://openalex.org/keywords/human-induced-pluripotent-stem-cells","display_name":"Human Induced Pluripotent Stem Cells","score":0.5278204083442688},{"id":"https://openalex.org/keywords/transplantation","display_name":"Transplantation","score":0.436431348323822},{"id":"https://openalex.org/keywords/cell-culture","display_name":"Cell culture","score":0.4268462359905243},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.4237414002418518},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.35024818778038025},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.3446460962295532},{"id":"https://openalex.org/keywords/embryonic-stem-cell","display_name":"Embryonic stem cell","score":0.22171056270599365},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.1677015721797943},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.16466370224952698},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.14170435070991516},{"id":"https://openalex.org/keywords/in-vitro","display_name":"In vitro","score":0.13253405690193176},{"id":"https://openalex.org/keywords/internal-medicine","display_name":"Internal medicine","score":0.06858181953430176}],"concepts":[{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.8482058048248291},{"id":"https://openalex.org/C2776200302","wikidata":"https://www.wikidata.org/wiki/Q827450","display_name":"Hepatocyte","level":3,"score":0.7926762104034424},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6812251806259155},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5663834810256958},{"id":"https://openalex.org/C2910924664","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Human Induced Pluripotent Stem Cells","level":5,"score":0.5278204083442688},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.436431348323822},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4268462359905243},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4237414002418518},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35024818778038025},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3446460962295532},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.22171056270599365},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1677015721797943},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16466370224952698},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.14170435070991516},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.13253405690193176},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.06858181953430176}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/mhs.2014.7006151","is_oa":false,"landing_page_url":"https://doi.org/10.1109/mhs.2014.7006151","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W2000151960","https://openalex.org/W2013620034","https://openalex.org/W2020755558","https://openalex.org/W2131635560","https://openalex.org/W2325963751"],"related_works":["https://openalex.org/W4212799663","https://openalex.org/W2913010565","https://openalex.org/W2150919128","https://openalex.org/W2348255200","https://openalex.org/W4387142161","https://openalex.org/W2131877082","https://openalex.org/W1972774086","https://openalex.org/W2098476050","https://openalex.org/W2021121983","https://openalex.org/W73715483"],"abstract_inverted_index":{"A":[0],"huge":[1],"number":[2],"of":[3,8,15,27,73,133],"publications":[4],"reported":[5],"the":[6,33,47,56,77],"production":[7,72],"immature":[9],"hepatocyte-like":[10,98],"cells":[11,42,74],"by":[12,131],"sequential":[13],"addition":[14],"multiple":[16],"inductive":[17],"factors;":[18],"however,":[19],"little":[20],"comparative":[21],"analyses":[22],"were":[23,81],"performed.":[24],"The":[25],"purpose":[26],"this":[28],"study":[29],"is":[30],"to":[31,54],"identify":[32],"optimal":[34],"induction":[35],"system":[36],"using":[37],"human":[38,119,129],"induced":[39],"pluripotent":[40],"stem":[41],"(iPSCs).":[43],"Initially,":[44],"based":[45],"on":[46],"published":[48],"outcomes,":[49],"functional":[50],"screen":[51],"was":[52],"performed":[53],"select":[55],"three":[57],"promising":[58],"step-wise":[59],"differentiation":[60],"protocols.":[61],"Then,":[62],"cellular":[63],"morphology,":[64],"hepatocyte":[65],"marker":[66],"gene":[67],"expressions,":[68],"and":[69],"secreted":[70],"protein":[71],"derived":[75,117],"from":[76,118],"each":[78],"specific":[79],"protocol":[80,95],"characterized":[82],"at":[83],"final":[84],"maturation":[85],"stage.":[86],"Through":[87],"these":[88],"efforts,":[89],"we":[90],"identified":[91],"a":[92],"highly":[93],"efficient":[94],"for":[96],"deriving":[97],"cells.":[99],"In":[100],"combination":[101],"with":[102,121],"our":[103],"recently":[104],"developed":[105],"3-D":[106],"culture":[107],"systems":[108],"(organ":[109],"bud":[110,135],"generation":[111],"method),":[112],"mature":[113],"hepatocytes":[114],"will":[115],"be":[116],"iPSCs":[120],"markedly":[122],"increased":[123],"efficiency":[124],"in":[125],"an":[126],"artificially":[127],"reconstituted":[128],"liver":[130,134],"way":[132],"transplantation.":[136]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}