{"id":"https://openalex.org/W3184506305","doi":"https://doi.org/10.1109/memea52024.2021.9478777","title":"Enhancing the Fluorescence and Cycle Threshold of qPCR Devices Through Excitation Time Point Adjustment","display_name":"Enhancing the Fluorescence and Cycle Threshold of qPCR Devices Through Excitation Time Point Adjustment","publication_year":2021,"publication_date":"2021-06-23","ids":{"openalex":"https://openalex.org/W3184506305","doi":"https://doi.org/10.1109/memea52024.2021.9478777","mag":"3184506305"},"language":"en","primary_location":{"id":"doi:10.1109/memea52024.2021.9478777","is_oa":false,"landing_page_url":"https://doi.org/10.1109/memea52024.2021.9478777","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2021 IEEE International Symposium on Medical Measurements and Applications (MeMeA)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056415038","display_name":"Hsin\u2010Yi Tsai","orcid":"https://orcid.org/0000-0001-8275-6132"},"institutions":[{"id":"https://openalex.org/I4210166867","display_name":"National Institutes of Applied Research","ror":"https://ror.org/05wcstg80","country_code":"TW","type":"government","lineage":["https://openalex.org/I4210128167","https://openalex.org/I4210166867"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Hsin-Yi Tsai","raw_affiliation_strings":["National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan","institution_ids":["https://openalex.org/I4210166867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053558043","display_name":"Liang-Chieh Chao","orcid":null},"institutions":[{"id":"https://openalex.org/I4210166867","display_name":"National Institutes of Applied Research","ror":"https://ror.org/05wcstg80","country_code":"TW","type":"government","lineage":["https://openalex.org/I4210128167","https://openalex.org/I4210166867"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Liang-Chieh Chao","raw_affiliation_strings":["National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan","institution_ids":["https://openalex.org/I4210166867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080419061","display_name":"Cheng-Ru Li","orcid":"https://orcid.org/0000-0001-6391-1563"},"institutions":[{"id":"https://openalex.org/I4210166867","display_name":"National Institutes of Applied Research","ror":"https://ror.org/05wcstg80","country_code":"TW","type":"government","lineage":["https://openalex.org/I4210128167","https://openalex.org/I4210166867"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Cheng-Ru Li","raw_affiliation_strings":["National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan","institution_ids":["https://openalex.org/I4210166867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101750297","display_name":"Kuo\u2010Cheng Huang","orcid":"https://orcid.org/0000-0001-9971-8343"},"institutions":[{"id":"https://openalex.org/I4210166867","display_name":"National Institutes of Applied Research","ror":"https://ror.org/05wcstg80","country_code":"TW","type":"government","lineage":["https://openalex.org/I4210128167","https://openalex.org/I4210166867"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Kuo-Cheng Huang","raw_affiliation_strings":["National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan","institution_ids":["https://openalex.org/I4210166867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110696559","display_name":"Yu\u2013Hsuan Lin","orcid":"https://orcid.org/0000-0002-1981-0359"},"institutions":[{"id":"https://openalex.org/I4210166867","display_name":"National Institutes of Applied Research","ror":"https://ror.org/05wcstg80","country_code":"TW","type":"government","lineage":["https://openalex.org/I4210128167","https://openalex.org/I4210166867"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Yu-Hsuan Lin","raw_affiliation_strings":["National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Applied Research Laboratories, Taiwan Instrument Research Institute, Hsinchu, Taiwan","institution_ids":["https://openalex.org/I4210166867"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018379827","display_name":"Dar\u2010Bin Shieh","orcid":null},"institutions":[{"id":"https://openalex.org/I91807558","display_name":"National Cheng Kung University","ror":"https://ror.org/01b8kcc49","country_code":"TW","type":"education","lineage":["https://openalex.org/I91807558"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Dar-Bin Shieh","raw_affiliation_strings":["Institute of Oral Medicine, School of Dentistry, National Cheng Kung University, Tainan, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Oral Medicine, School of Dentistry, National Cheng Kung University, Tainan, Taiwan","institution_ids":["https://openalex.org/I91807558"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0814,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.43843452,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9991000294685364,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.8135699033737183},{"id":"https://openalex.org/keywords/excitation","display_name":"Excitation","score":0.7279400825500488},{"id":"https://openalex.org/keywords/fluorescence-correlation-spectroscopy","display_name":"Fluorescence correlation spectroscopy","score":0.5729159712791443},{"id":"https://openalex.org/keywords/intensity","display_name":"Intensity (physics)","score":0.5148527026176453},{"id":"https://openalex.org/keywords/fluorescence-spectroscopy","display_name":"Fluorescence spectroscopy","score":0.4577447772026062},{"id":"https://openalex.org/keywords/analytical-chemistry","display_name":"Analytical Chemistry (journal)","score":0.4293840527534485},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.4100739061832428},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.40230199694633484},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.255318820476532},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.1541060209274292},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.11890062689781189}],"concepts":[{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.8135699033737183},{"id":"https://openalex.org/C83581075","wikidata":"https://www.wikidata.org/wiki/Q1361503","display_name":"Excitation","level":2,"score":0.7279400825500488},{"id":"https://openalex.org/C144618238","wikidata":"https://www.wikidata.org/wiki/Q902601","display_name":"Fluorescence correlation spectroscopy","level":3,"score":0.5729159712791443},{"id":"https://openalex.org/C93038891","wikidata":"https://www.wikidata.org/wiki/Q1061524","display_name":"Intensity (physics)","level":2,"score":0.5148527026176453},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.4577447772026062},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.4293840527534485},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4100739061832428},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.40230199694633484},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.255318820476532},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1541060209274292},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.11890062689781189},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/memea52024.2021.9478777","is_oa":false,"landing_page_url":"https://doi.org/10.1109/memea52024.2021.9478777","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2021 IEEE International Symposium on Medical Measurements and Applications (MeMeA)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.47999998927116394,"display_name":"Affordable and clean energy","id":"https://metadata.un.org/sdg/7"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320322108","display_name":"Ministry of Science and Technology","ror":"https://ror.org/032e49973"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W2022227920","https://openalex.org/W2070721758","https://openalex.org/W2099155118","https://openalex.org/W2110168898","https://openalex.org/W2115513841","https://openalex.org/W2129109142","https://openalex.org/W2149551170","https://openalex.org/W2158894328","https://openalex.org/W2164095252","https://openalex.org/W2295770661","https://openalex.org/W2409289937","https://openalex.org/W2412578649","https://openalex.org/W2578588363","https://openalex.org/W2584810127","https://openalex.org/W3042073869","https://openalex.org/W3042099287","https://openalex.org/W3088011310"],"related_works":["https://openalex.org/W2137500070","https://openalex.org/W2095088622","https://openalex.org/W2361503254","https://openalex.org/W2172005118","https://openalex.org/W2070167001","https://openalex.org/W2015512651","https://openalex.org/W2791096033","https://openalex.org/W2090787555","https://openalex.org/W2468630222","https://openalex.org/W2033084752"],"abstract_inverted_index":{"Quantitative":[0],"polymerase":[1],"chain":[2],"reaction":[3,98,157],"(qPCR)":[4],"has":[5],"been":[6],"widely":[7],"employed":[8],"for":[9,29,82,117],"the":[10,30,46,61,92,97,101,128,144,150,163,192,200],"positive":[11,31],"or":[12,17],"negative":[13,33],"detection":[14,34],"of":[15,35,49,64,100,195,205],"bacteria":[16],"viruses,":[18],"particularly":[19],"SARS-CoV-2.":[20],"Fluorescence":[21,124],"signal":[22],"and":[23,32,67,96,113,143,162,167,180,203,214],"cycle":[24,51,169],"threshold":[25,170],"information":[26],"is":[27],"critical":[28],"target":[36],"test":[37],"samples":[38],"in":[39,109,120,127,211],"qPCR":[40,57,122,206],"systems.":[41],"To":[42],"determine":[43],"viral":[44,216],"concentration,":[45],"fluorescence":[47,65,72,84,111,118,131,165,178,201],"intensity":[48,70,125],"each":[50,104],"must":[52],"be":[53],"recorded":[54],"using":[55,138],"a":[56,121,139],"system.":[58,123],"In":[59],"general,":[60],"time":[62,94,99,158,193],"points":[63],"excitation":[66,68,80,89,93,119,134,145,196],"light":[69,90,146,182,197],"affect":[71],"intensity.":[73,85],"Thus,":[74],"this":[75],"study":[76],"proposed":[77],"an":[78],"effective":[79],"method":[81],"enhancing":[83],"Several":[86],"parameters,":[87],"including":[88],"intensity,":[91],"point,":[95],"reagent":[102],"at":[103],"temperature":[105,151],"stage,":[106],"were":[107,172,184],"modified":[108],"assessing":[110],"performance":[112,204],"determining":[114],"suitable":[115,168],"parameters":[116],"resulted":[126],"most":[129],"optimal":[130],"performance;":[132],"specifically,":[133],"was":[135,147,159],"triggered":[136],"by":[137],"30":[140],"mA":[141],"current,":[142],"activated":[148],"when":[149],"decreased":[152],"to":[153],"60":[154],"\u00b0C.":[155],"Total":[156],"1":[160],"s,":[161],"concentrated":[164],"value":[166,171],"obtained.":[173],"Overall,":[174],"high":[175,181],"efficiency,":[176],"low":[177],"decay,":[179],"stability":[183],"observed.":[185],"The":[186],"present":[187],"findings":[188],"demonstrate":[189],"that":[190],"controlling":[191],"point":[194],"can":[198],"enhance":[199],"efficiency":[202],"systems,":[207],"with":[208],"relevant":[209],"benefits":[210],"medical":[212],"diagnostics":[213],"rapid":[215],"detection,":[217],"among":[218],"other":[219],"applications.":[220]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-06-11T09:08:48.828518","created_date":"2025-10-10T00:00:00"}