{"id":"https://openalex.org/W4288389000","doi":"https://doi.org/10.1109/jcsse54890.2022.9836243","title":"Quantitative analysis of the 2D tissue skin layer with fluorescent dyes","display_name":"Quantitative analysis of the 2D tissue skin layer with fluorescent dyes","publication_year":2022,"publication_date":"2022-06-22","ids":{"openalex":"https://openalex.org/W4288389000","doi":"https://doi.org/10.1109/jcsse54890.2022.9836243"},"language":"en","primary_location":{"id":"doi:10.1109/jcsse54890.2022.9836243","is_oa":false,"landing_page_url":"https://doi.org/10.1109/jcsse54890.2022.9836243","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2022 19th International Joint Conference on Computer Science and Software Engineering (JCSSE)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051881603","display_name":"Rachata Chaiprasongsuk","orcid":null},"institutions":[{"id":"https://openalex.org/I1332387789","display_name":"Siriraj Hospital","ror":"https://ror.org/0331zs648","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I1332387789"]},{"id":"https://openalex.org/I198105771","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21","country_code":"TH","type":"education","lineage":["https://openalex.org/I198105771"]},{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":true,"raw_author_name":"Rachata Chaiprasongsuk","raw_affiliation_strings":["Kasetsart University, Mahidol University,Faculty of Engineering, Faculty of Medicine Siriraj Hospital,Department of Mechanical Engineering, Department of Surgery,Bangkok,Thailand","Department of Mechanical Engineering, Department of Surgery, Faculty of Engineering, Faculty of Medicine Siriraj Hospital, Kasetsart University, Mahidol University, Bangkok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kasetsart University, Mahidol University,Faculty of Engineering, Faculty of Medicine Siriraj Hospital,Department of Mechanical Engineering, Department of Surgery,Bangkok,Thailand","institution_ids":["https://openalex.org/I1332387789"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Department of Surgery, Faculty of Engineering, Faculty of Medicine Siriraj Hospital, Kasetsart University, Mahidol University, Bangkok, Thailand","institution_ids":["https://openalex.org/I1332387789","https://openalex.org/I198105771","https://openalex.org/I25399158"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069981232","display_name":"Paniti Achararit","orcid":"https://orcid.org/0000-0003-2753-7580"},"institutions":[{"id":"https://openalex.org/I4210106686","display_name":"Chulabhorn Hospital","ror":"https://ror.org/01qc5zk84","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I4210106686"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Paniti Achararit","raw_affiliation_strings":["Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","institution_ids":["https://openalex.org/I4210106686"]},{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand","institution_ids":["https://openalex.org/I4210106686"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056364313","display_name":"Pasit Jarutatsanangkoon","orcid":null},"institutions":[{"id":"https://openalex.org/I4210106686","display_name":"Chulabhorn Hospital","ror":"https://ror.org/01qc5zk84","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I4210106686"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pasit Jarutatsanangkoon","raw_affiliation_strings":["Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","institution_ids":["https://openalex.org/I4210106686"]},{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand","institution_ids":["https://openalex.org/I4210106686"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040780882","display_name":"Pawaree Nonthasaen","orcid":"https://orcid.org/0000-0001-7044-9063"},"institutions":[{"id":"https://openalex.org/I4210106686","display_name":"Chulabhorn Hospital","ror":"https://ror.org/01qc5zk84","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I4210106686"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pawaree Nonthasaen","raw_affiliation_strings":["Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","institution_ids":["https://openalex.org/I4210106686"]},{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand","institution_ids":["https://openalex.org/I4210106686"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035499081","display_name":"Wiriya Mahikul","orcid":"https://orcid.org/0000-0001-5834-5173"},"institutions":[{"id":"https://openalex.org/I4210106686","display_name":"Chulabhorn Hospital","ror":"https://ror.org/01qc5zk84","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I4210106686"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Wiriya Mahikul","raw_affiliation_strings":["Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand"],"raw_orcid":"https://orcid.org/0000-0001-5834-5173","affiliations":[{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","institution_ids":["https://openalex.org/I4210106686"]},{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand","institution_ids":["https://openalex.org/I4210106686"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027885683","display_name":"Anyamanee Chaiprasongsuk","orcid":"https://orcid.org/0000-0003-0428-0778"},"institutions":[{"id":"https://openalex.org/I4210106686","display_name":"Chulabhorn Hospital","ror":"https://ror.org/01qc5zk84","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I4210106686"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Anyamanee Chaiprasongsuk","raw_affiliation_strings":["Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand"],"raw_orcid":"https://orcid.org/0000-0003-0428-0778","affiliations":[{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy,Bangkok,Thailand","institution_ids":["https://openalex.org/I4210106686"]},{"raw_affiliation_string":"Princess Srisavangavadhana College of Medicine Chulabhorn Royal Academy, Bangkok, Thailand","institution_ids":["https://openalex.org/I4210106686"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046761489","display_name":"Uraiwan Panich","orcid":"https://orcid.org/0000-0002-4889-6729"},"institutions":[{"id":"https://openalex.org/I1332387789","display_name":"Siriraj Hospital","ror":"https://ror.org/0331zs648","country_code":"TH","type":"healthcare","lineage":["https://openalex.org/I1332387789"]},{"id":"https://openalex.org/I25399158","display_name":"Mahidol University","ror":"https://ror.org/01znkr924","country_code":"TH","type":"education","lineage":["https://openalex.org/I25399158"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Uraiwan Panich","raw_affiliation_strings":["Mahidol University,Faculty of Medicine Siriraj Hospital,Department of Pharmacology,Bangkok,Thailand","Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand"],"raw_orcid":"https://orcid.org/0000-0002-4889-6729","affiliations":[{"raw_affiliation_string":"Mahidol University,Faculty of Medicine Siriraj Hospital,Department of Pharmacology,Bangkok,Thailand","institution_ids":["https://openalex.org/I1332387789"]},{"raw_affiliation_string":"Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand","institution_ids":["https://openalex.org/I1332387789","https://openalex.org/I25399158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028152905","display_name":"Pongtorn Prombut","orcid":null},"institutions":[{"id":"https://openalex.org/I198105771","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21","country_code":"TH","type":"education","lineage":["https://openalex.org/I198105771"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pongtorn Prombut","raw_affiliation_strings":["Kasetsart University,Faculty of Engineering,Department of Mechanical Engineering,Bangkok,Thailand","Department of Mechanical Engineering, Faculty of Engineering, Kasetsart University, Bangkok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kasetsart University,Faculty of Engineering,Department of Mechanical Engineering,Bangkok,Thailand","institution_ids":["https://openalex.org/I198105771"]},{"raw_affiliation_string":"Department of Mechanical Engineering, Faculty of Engineering, Kasetsart University, Bangkok, Thailand","institution_ids":["https://openalex.org/I198105771"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5051881603"],"corresponding_institution_ids":["https://openalex.org/I1332387789","https://openalex.org/I198105771","https://openalex.org/I25399158"],"apc_list":null,"apc_paid":null,"fwci":0.5281,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.78508105,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":95},"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9983999729156494,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9983999729156494,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9873999953269958,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9621999859809875,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/grayscale","display_name":"Grayscale","score":0.8909836411476135},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.636620044708252},{"id":"https://openalex.org/keywords/rgb-color-model","display_name":"RGB color model","score":0.5352457761764526},{"id":"https://openalex.org/keywords/software","display_name":"Software","score":0.5332574248313904},{"id":"https://openalex.org/keywords/pixel","display_name":"Pixel","score":0.5321630239486694},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.523908257484436},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.49750903248786926},{"id":"https://openalex.org/keywords/process","display_name":"Process (computing)","score":0.46491605043411255},{"id":"https://openalex.org/keywords/biomedical-engineering","display_name":"Biomedical engineering","score":0.4449199140071869},{"id":"https://openalex.org/keywords/image-processing","display_name":"Image processing","score":0.4353005886077881},{"id":"https://openalex.org/keywords/intensity","display_name":"Intensity (physics)","score":0.4201992154121399},{"id":"https://openalex.org/keywords/image","display_name":"Image (mathematics)","score":0.18549072742462158},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.16187143325805664},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.0988190770149231},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.09009683132171631}],"concepts":[{"id":"https://openalex.org/C78201319","wikidata":"https://www.wikidata.org/wiki/Q685727","display_name":"Grayscale","level":3,"score":0.8909836411476135},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.636620044708252},{"id":"https://openalex.org/C82990744","wikidata":"https://www.wikidata.org/wiki/Q166194","display_name":"RGB color model","level":2,"score":0.5352457761764526},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.5332574248313904},{"id":"https://openalex.org/C160633673","wikidata":"https://www.wikidata.org/wiki/Q355198","display_name":"Pixel","level":2,"score":0.5321630239486694},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.523908257484436},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.49750903248786926},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.46491605043411255},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.4449199140071869},{"id":"https://openalex.org/C9417928","wikidata":"https://www.wikidata.org/wiki/Q1070689","display_name":"Image processing","level":3,"score":0.4353005886077881},{"id":"https://openalex.org/C93038891","wikidata":"https://www.wikidata.org/wiki/Q1061524","display_name":"Intensity (physics)","level":2,"score":0.4201992154121399},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.18549072742462158},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.16187143325805664},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0988190770149231},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09009683132171631},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/jcsse54890.2022.9836243","is_oa":false,"landing_page_url":"https://doi.org/10.1109/jcsse54890.2022.9836243","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2022 19th International Joint Conference on Computer Science and Software Engineering (JCSSE)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322401","display_name":"Mahidol University","ror":"https://ror.org/01znkr924"},{"id":"https://openalex.org/F4320322722","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1166534496","https://openalex.org/W1515135833","https://openalex.org/W1994576750","https://openalex.org/W2006366433","https://openalex.org/W2008568038","https://openalex.org/W2015159529","https://openalex.org/W2082454765","https://openalex.org/W2097524851","https://openalex.org/W2105956662","https://openalex.org/W2124160079","https://openalex.org/W2162629747","https://openalex.org/W2563575197","https://openalex.org/W2793685996","https://openalex.org/W2801422562","https://openalex.org/W2979896223","https://openalex.org/W3104927021","https://openalex.org/W3177818662","https://openalex.org/W4231627457","https://openalex.org/W4250282305"],"related_works":["https://openalex.org/W115686965","https://openalex.org/W2768918307","https://openalex.org/W2110031805","https://openalex.org/W2040020606","https://openalex.org/W4362659915","https://openalex.org/W3153082147","https://openalex.org/W1691631808","https://openalex.org/W2899689856","https://openalex.org/W1586320973","https://openalex.org/W2968833425"],"abstract_inverted_index":{"Development":[0],"of":[1,11,20,32,42,85,122,218],"an":[2,129,238],"automated":[3,226],"process":[4,112,131,149],"for":[5,47,82,135,196,246],"quantifying":[6],"proteins":[7],"in":[8,17],"multiple":[9],"layers":[10],"skin":[12],"is":[13,40,104,189],"essential":[14],"to":[15,25,73,125,139,177,191,209,214,221,241],"research":[16],"the":[18,27,43,48,54,58,63,75,83,90,97,102,136,147,152,158,182,193,197,211,216,230,243],"field":[19],"cell":[21,28,247],"biology.":[22],"Researchers":[23],"aimed":[24],"quantify":[26,74],"density":[29],"and":[30,99,142,155,161,233],"levels":[31],"biomarkers":[33],"on":[34,96,173,181],"tissue":[35,38,244],"samples.":[36],"Immunofluorescence":[37],"staining":[39,188],"one":[41],"standard":[44],"methods":[45],"used":[46],"protein":[49,76],"detection":[50],"by":[51,150],"tagging":[52],"with":[53,165],"fluorescein":[55],"isothiocyanate":[56],"(FITC),":[57],"green":[59],"fluorescent":[60],"agent.":[61],"Recently,":[62],"single-cell":[64],"(monolayer":[65],"tissue)":[66],"analysis":[67,84,92,103,138,245],"software":[68,110],"automation":[69],"has":[70,132],"been":[71,133],"obtained":[72],"levels.":[77],"However,":[78],"there":[79],"are":[80],"limitations":[81],"overlapping":[86],"multilayer":[87],"tissue.":[88],"Since":[89],"automatic":[91,130,148],"cannot":[93],"be":[94,126,171,192,210],"operated":[95],"rough":[98],"muti-layered":[100],"surface,":[101],"then":[105],"manually":[106],"performed":[107],"using":[108],"ImageJ":[109],"which":[111,236],"more":[113],"than":[114],"5":[115],"minutes/image.":[116],"Problems":[117],"arise":[118],"when":[119],"many":[120],"images":[121],"tissues":[123],"need":[124],"analyzed.":[127],"Therefore,":[128,224],"developed":[134,146],"image":[137],"reduce":[140],"time":[141],"error.":[143],"This":[144],"study":[145],"adjusting":[151],"pixel":[153,198],"intensity":[154,169,195,199],"splitting":[156],"into":[157,163],"RGB":[159],"channels":[160],"converting":[162],"gray":[164,202],"different":[166],"intensities.":[167],"The":[168,186,201],"can":[170],"measured":[172],"grayscale":[174,184,194],"from":[175],"0":[176],"255":[178],"units":[179,206],"based":[180],"KODAK":[183],"zone.":[185],"FITC":[187],"converted":[190],"calculation.":[200],"level":[203],"<":[204],"25":[205],"was":[207],"setup":[208],"exclusion":[212],"criteria":[213],"solve":[215],"problem":[217],"over-exposure":[219],"due":[220],"background":[222],"noise.":[223],"this":[225],"data":[227],"processing":[228],"enhances":[229],"technical":[231],"capabilities":[232],"computational":[234],"accuracy,":[235],"proposes":[237],"alternative":[239],"approach":[240],"improve":[242],"biology":[248],"research.":[249]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}