{"id":"https://openalex.org/W4386352550","doi":"https://doi.org/10.1109/isbi53787.2023.10230449","title":"Three-Dimensional Nuclei Synthesis for Fluorescence Microscopy Image Analysis","display_name":"Three-Dimensional Nuclei Synthesis for Fluorescence Microscopy Image Analysis","publication_year":2023,"publication_date":"2023-04-18","ids":{"openalex":"https://openalex.org/W4386352550","doi":"https://doi.org/10.1109/isbi53787.2023.10230449"},"language":"en","primary_location":{"id":"doi:10.1109/isbi53787.2023.10230449","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1109/isbi53787.2023.10230449","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2023 IEEE 20th International Symposium on Biomedical Imaging (ISBI)","raw_type":"proceedings-article"},"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078509204","display_name":"Liming Wu","orcid":"https://orcid.org/0000-0002-5738-0468"},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Liming Wu","raw_affiliation_strings":["Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006890201","display_name":"Alain Chen","orcid":"https://orcid.org/0000-0001-7119-0815"},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alain Chen","raw_affiliation_strings":["Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026393791","display_name":"Paul Salama","orcid":"https://orcid.org/0000-0002-7643-3879"},"institutions":[{"id":"https://openalex.org/I135191193","display_name":"University of Indianapolis","ror":"https://ror.org/052133d12","country_code":"US","type":"education","lineage":["https://openalex.org/I135191193"]},{"id":"https://openalex.org/I55769427","display_name":"Indiana University \u2013 Purdue University Indianapolis","ror":"https://ror.org/05gxnyn08","country_code":"US","type":"education","lineage":["https://openalex.org/I55769427","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul Salama","raw_affiliation_strings":["Indiana University-Purdue University,Department of Electrical and Computer Engineering,Indianapolis,Indiana,USA","Department of Electrical and Computer Engineering, Indiana University-Purdue University, Indianapolis, Indiana, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University-Purdue University,Department of Electrical and Computer Engineering,Indianapolis,Indiana,USA","institution_ids":["https://openalex.org/I135191193","https://openalex.org/I55769427"]},{"raw_affiliation_string":"Department of Electrical and Computer Engineering, Indiana University-Purdue University, Indianapolis, Indiana, USA","institution_ids":["https://openalex.org/I55769427"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070413882","display_name":"Kenneth W. Dunn","orcid":"https://orcid.org/0000-0003-4245-496X"},"institutions":[{"id":"https://openalex.org/I55769427","display_name":"Indiana University \u2013 Purdue University Indianapolis","ror":"https://ror.org/05gxnyn08","country_code":"US","type":"education","lineage":["https://openalex.org/I55769427","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth W. Dunn","raw_affiliation_strings":["Indiana University,Division of Nephrology School of Medicine,Indianapolis,Indiana,USA","Division of Nephrology School of Medicine, Indiana University, Indianapolis, Indiana, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University,Division of Nephrology School of Medicine,Indianapolis,Indiana,USA","institution_ids":["https://openalex.org/I55769427"]},{"raw_affiliation_string":"Division of Nephrology School of Medicine, Indiana University, Indianapolis, Indiana, USA","institution_ids":["https://openalex.org/I55769427"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089688702","display_name":"Edward J. Delp","orcid":"https://orcid.org/0000-0002-2909-7323"},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Edward J. Delp","raw_affiliation_strings":["Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Purdue University,Video and Image Processing Laboratory, School of Electrical and Computer Engineering,West Lafayette,Indiana,USA","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Video and Image Processing Laboratory, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana, USA","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"30","issue":null,"first_page":"1","last_page":"5"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9993000030517578,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9993000030517578,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10862","display_name":"AI in cancer detection","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1702","display_name":"Artificial Intelligence"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10531","display_name":"Advanced Vision and Imaging","score":0.9933000206947327,"subfield":{"id":"https://openalex.org/subfields/1707","display_name":"Computer Vision and Pattern Recognition"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.7736712694168091},{"id":"https://openalex.org/keywords/segmentation","display_name":"Segmentation","score":0.7714732885360718},{"id":"https://openalex.org/keywords/ground-truth","display_name":"Ground truth","score":0.7679957151412964},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.7393670678138733},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6527889370918274},{"id":"https://openalex.org/keywords/fluorescence-microscope","display_name":"Fluorescence microscope","score":0.6252642869949341},{"id":"https://openalex.org/keywords/deep-learning","display_name":"Deep learning","score":0.5768991112709045},{"id":"https://openalex.org/keywords/image-segmentation","display_name":"Image segmentation","score":0.559330940246582},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.5224632024765015},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.4697841703891754},{"id":"https://openalex.org/keywords/pattern-recognition","display_name":"Pattern recognition (psychology)","score":0.4218355715274811},{"id":"https://openalex.org/keywords/cytometry","display_name":"Cytometry","score":0.4147692024707794},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.21939942240715027},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.1989399492740631},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"Flow cytometry","score":0.17323288321495056},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.12299180030822754},{"id":"https://openalex.org/keywords/molecular-biology","display_name":"Molecular biology","score":0.07397574186325073}],"concepts":[{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.7736712694168091},{"id":"https://openalex.org/C89600930","wikidata":"https://www.wikidata.org/wiki/Q1423946","display_name":"Segmentation","level":2,"score":0.7714732885360718},{"id":"https://openalex.org/C146849305","wikidata":"https://www.wikidata.org/wiki/Q370766","display_name":"Ground truth","level":2,"score":0.7679957151412964},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.7393670678138733},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6527889370918274},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.6252642869949341},{"id":"https://openalex.org/C108583219","wikidata":"https://www.wikidata.org/wiki/Q197536","display_name":"Deep learning","level":2,"score":0.5768991112709045},{"id":"https://openalex.org/C124504099","wikidata":"https://www.wikidata.org/wiki/Q56933","display_name":"Image segmentation","level":3,"score":0.559330940246582},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.5224632024765015},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4697841703891754},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.4218355715274811},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.4147692024707794},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.21939942240715027},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1989399492740631},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.17323288321495056},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.12299180030822754},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.07397574186325073}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/isbi53787.2023.10230449","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1109/isbi53787.2023.10230449","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2023 IEEE 20th International Symposium on Biomedical Imaging (ISBI)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6299999952316284,"display_name":"Decent work and economic growth","id":"https://metadata.un.org/sdg/8"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320338440","display_name":"HORIZON EUROPE Health","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1976526581","https://openalex.org/W2070008638","https://openalex.org/W2105456967","https://openalex.org/W2156163116","https://openalex.org/W2194775991","https://openalex.org/W2736597453","https://openalex.org/W2798976889","https://openalex.org/W2962793481","https://openalex.org/W2963073614","https://openalex.org/W2991915699","https://openalex.org/W3021714323","https://openalex.org/W3099064910","https://openalex.org/W3111521801","https://openalex.org/W3118337643","https://openalex.org/W3165489647","https://openalex.org/W3216522454","https://openalex.org/W4212875960","https://openalex.org/W4282585107","https://openalex.org/W4289774685","https://openalex.org/W4292828884","https://openalex.org/W4297312588","https://openalex.org/W4301206121","https://openalex.org/W6765779288","https://openalex.org/W6838594866","https://openalex.org/W6842521865"],"related_works":["https://openalex.org/W4211107619","https://openalex.org/W2161433272","https://openalex.org/W2894139091","https://openalex.org/W1637909","https://openalex.org/W1983780159","https://openalex.org/W659245948","https://openalex.org/W2094643072","https://openalex.org/W2087957833","https://openalex.org/W4206210906","https://openalex.org/W4251922430"],"abstract_inverted_index":{"Three-dimensional":[0],"tissue":[1,29],"cytometry":[2],"is":[3,23,53],"an":[4,24],"important":[5,25],"technique":[6],"for":[7,27,38,48,58,75,128],"quantitative":[8],"analysis":[9],"of":[10],"cell":[11],"structures":[12],"in":[13],"large":[14,59],"fluorescence":[15,90],"microscopy":[16,85,91],"volumes.":[17,61,86],"Accurate":[18],"nuclei":[19,39,69],"detection":[20,40],"and":[21,41,55,106],"segmentation":[22,126,131],"step":[26],"3D":[28,60,68,77,100],"cytometry.":[30],"Deep":[31],"learning":[32,51,130],"methods":[33,52],"have":[34],"shown":[35],"promising":[36],"results":[37,88],"segmentation.":[42],"However,":[43],"manually":[44],"annotating":[45],"ground":[46,78],"truth":[47,79],"training":[49,123],"deep":[50,129],"labor-intensive":[54],"not":[56],"practical":[57],"In":[62],"this":[63],"paper,":[64],"we":[65],"propose":[66],"a":[67],"synthesis":[70],"method,":[71],"known":[72],"as":[73,122],"3DSpCycleGAN,":[74],"generating":[76],"volumes":[80,92,101,118],"along":[81],"with":[82],"corresponding":[83],"synthetic":[84,117],"Experimental":[87],"using":[89,115],"demonstrate":[93],"that":[94,114],"our":[95],"method":[96],"generates":[97],"more":[98],"realistic":[99],"when":[102],"evaluated":[103],"both":[104],"visually":[105],"quantitatively":[107],"than":[108],"previously":[109],"reported.":[110],"We":[111],"also":[112],"show":[113],"the":[116],"generated":[119],"by":[120],"3DSpCycleGAN":[121],"data":[124],"improves":[125],"accuracy":[127],"techniques.":[132]},"counts_by_year":[],"updated_date":"2026-07-22T07:51:19.307946","created_date":"2025-10-10T00:00:00"}