{"id":"https://openalex.org/W2039070119","doi":"https://doi.org/10.1109/isbi.2009.5193323","title":"Identification of cellular dynamic patterns resulting from repetitive photobleaching using Independent Component Analysis","display_name":"Identification of cellular dynamic patterns resulting from repetitive photobleaching using Independent Component Analysis","publication_year":2009,"publication_date":"2009-06-01","ids":{"openalex":"https://openalex.org/W2039070119","doi":"https://doi.org/10.1109/isbi.2009.5193323","mag":"2039070119"},"language":"en","primary_location":{"id":"doi:10.1109/isbi.2009.5193323","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2009.5193323","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065814741","display_name":"Alice M. C. van Gemert","orcid":null},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"A.M.C. van Gemert","raw_affiliation_strings":["Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028647030","display_name":"Carolina R. Jost","orcid":"https://orcid.org/0000-0001-8342-1094"},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"C.R. Jost","raw_affiliation_strings":["Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058149296","display_name":"Annelies M. A. van der Laan","orcid":"https://orcid.org/0000-0002-6104-2410"},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"A.M.A. van der Laan","raw_affiliation_strings":["Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110025160","display_name":"R.W. Dirks","orcid":null},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"R.W. Dirks","raw_affiliation_strings":["Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028379363","display_name":"Johan H. C. Reiber","orcid":"https://orcid.org/0000-0002-0387-6417"},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"J. H. C. Reiber","raw_affiliation_strings":["Division of Image Processing, Leiden University Medical Center, Leiden, Netherlands"],"affiliations":[{"raw_affiliation_string":"Division of Image Processing, Leiden University Medical Center, Leiden, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083219290","display_name":"Hans J. Tanke","orcid":null},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"H.J. Tanke","raw_affiliation_strings":["Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Molecular Cell Biology, Leiden University Medical Center, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019003563","display_name":"Boudewijn P. F. Lelieveldt","orcid":"https://orcid.org/0000-0001-8269-7603"},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]},{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"B. P. F. Lelieveldt","raw_affiliation_strings":["Department of Mediamatics, Delft University of Technnology, Delft, Netherlands","Division of Image Processing, Leiden University Medical Center, Leiden, Netherlands"],"affiliations":[{"raw_affiliation_string":"Department of Mediamatics, Delft University of Technnology, Delft, Netherlands","institution_ids":["https://openalex.org/I98358874"]},{"raw_affiliation_string":"Division of Image Processing, Leiden University Medical Center, Leiden, Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5065814741"],"corresponding_institution_ids":["https://openalex.org/I2800006345"],"apc_list":null,"apc_paid":null,"fwci":0.7049,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.67135706,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"27","issue":null,"first_page":"1382","last_page":"1385"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11447","display_name":"Blind Source Separation Techniques","score":0.9993000030517578,"subfield":{"id":"https://openalex.org/subfields/1711","display_name":"Signal Processing"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11447","display_name":"Blind Source Separation Techniques","score":0.9993000030517578,"subfield":{"id":"https://openalex.org/subfields/1711","display_name":"Signal Processing"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10640","display_name":"Spectroscopy and Chemometric Analyses","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9894999861717224,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/photobleaching","display_name":"Photobleaching","score":0.9237868785858154},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.7751583456993103},{"id":"https://openalex.org/keywords/component","display_name":"Component (thermodynamics)","score":0.7030878067016602},{"id":"https://openalex.org/keywords/fluorescence-recovery-after-photobleaching","display_name":"Fluorescence recovery after photobleaching","score":0.70073002576828},{"id":"https://openalex.org/keywords/independent-component-analysis","display_name":"Independent component analysis","score":0.5090743899345398},{"id":"https://openalex.org/keywords/dynamic-range","display_name":"Dynamic range","score":0.5040311217308044},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.48066914081573486},{"id":"https://openalex.org/keywords/interpretation","display_name":"Interpretation (philosophy)","score":0.4745386838912964},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.4506974518299103},{"id":"https://openalex.org/keywords/function","display_name":"Function (biology)","score":0.4390489459037781},{"id":"https://openalex.org/keywords/flip","display_name":"Flip","score":0.41910386085510254},{"id":"https://openalex.org/keywords/sequence","display_name":"Sequence (biology)","score":0.41785967350006104},{"id":"https://openalex.org/keywords/identification","display_name":"Identification (biology)","score":0.4102891683578491},{"id":"https://openalex.org/keywords/biophysics","display_name":"Biophysics","score":0.35551220178604126},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.33008432388305664},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.2863413095474243},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.23917004466056824},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.2167229950428009},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.155085951089859},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.1518154740333557},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.08552619814872742}],"concepts":[{"id":"https://openalex.org/C31332276","wikidata":"https://www.wikidata.org/wiki/Q899750","display_name":"Photobleaching","level":3,"score":0.9237868785858154},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.7751583456993103},{"id":"https://openalex.org/C168167062","wikidata":"https://www.wikidata.org/wiki/Q1117970","display_name":"Component (thermodynamics)","level":2,"score":0.7030878067016602},{"id":"https://openalex.org/C26659971","wikidata":"https://www.wikidata.org/wiki/Q729923","display_name":"Fluorescence recovery after photobleaching","level":3,"score":0.70073002576828},{"id":"https://openalex.org/C51432778","wikidata":"https://www.wikidata.org/wiki/Q1259145","display_name":"Independent component analysis","level":2,"score":0.5090743899345398},{"id":"https://openalex.org/C87133666","wikidata":"https://www.wikidata.org/wiki/Q1161699","display_name":"Dynamic range","level":2,"score":0.5040311217308044},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.48066914081573486},{"id":"https://openalex.org/C527412718","wikidata":"https://www.wikidata.org/wiki/Q855395","display_name":"Interpretation (philosophy)","level":2,"score":0.4745386838912964},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4506974518299103},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.4390489459037781},{"id":"https://openalex.org/C2776591724","wikidata":"https://www.wikidata.org/wiki/Q5459651","display_name":"Flip","level":3,"score":0.41910386085510254},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.41785967350006104},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.4102891683578491},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.35551220178604126},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33008432388305664},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.2863413095474243},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.23917004466056824},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.2167229950428009},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.155085951089859},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.1518154740333557},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08552619814872742},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/isbi.2009.5193323","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2009.5193323","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W85002878","https://openalex.org/W1914250865","https://openalex.org/W1966897054","https://openalex.org/W2019502123","https://openalex.org/W2123649031","https://openalex.org/W2163619454","https://openalex.org/W2170976240"],"related_works":["https://openalex.org/W1518240974","https://openalex.org/W2410074698","https://openalex.org/W1997452518","https://openalex.org/W113297786","https://openalex.org/W1978769331","https://openalex.org/W2140320154","https://openalex.org/W2143611296","https://openalex.org/W2789311093","https://openalex.org/W1574396544","https://openalex.org/W1964678604"],"abstract_inverted_index":{"Fluorescence":[0],"loss":[1],"in":[2,17,32],"photobleaching":[3],"(FLIP)":[4],"experiments":[5,34],"are":[6],"used":[7],"to":[8,25],"determine":[9],"the":[10,27,42,62,70,77],"dynamic":[11,30,50,86],"properties":[12],"of":[13,29,64,72,79],"fluorescently":[14],"labeled":[15],"macromolecules":[16],"cells.":[18],"This":[19],"paper":[20],"presents":[21],"a":[22,46,49,55],"novel":[23],"method":[24],"facilitate":[26],"interpretation":[28,71],"patterns":[31],"FLIP":[33,73],"based":[35],"on":[36],"independent":[37],"component":[38,65],"analysis.":[39],"By":[40],"decomposing":[41],"image":[43],"sequence":[44],"into":[45],"stationary":[47],"and":[48,52,66],"component,":[51],"by":[53,75],"deriving":[54],"bleaching":[56,67],"contrast":[57],"function,":[58],"we":[59],"demonstrate":[60],"that":[61],"triplet":[63],"images":[68],"enhances":[69],"sequences":[74],"outlining":[76],"movement":[78],"two":[80],"fluorescent":[81],"proteins":[82],"with":[83],"well":[84],"defined":[85],"characteristics.":[87]},"counts_by_year":[{"year":2012,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}