{"id":"https://openalex.org/W2118016638","doi":"https://doi.org/10.1109/isbi.2008.4541119","title":"Automated evaluation of HER-2/neu immunohistochemical expression in breast cancer using digital microscopy","display_name":"Automated evaluation of HER-2/neu immunohistochemical expression in breast cancer using digital microscopy","publication_year":2008,"publication_date":"2008-05-01","ids":{"openalex":"https://openalex.org/W2118016638","doi":"https://doi.org/10.1109/isbi.2008.4541119","mag":"2118016638"},"language":"en","primary_location":{"id":"doi:10.1109/isbi.2008.4541119","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2008.4541119","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039348485","display_name":"Marios A. Gavrielides","orcid":null},"institutions":[{"id":"https://openalex.org/I1335686170","display_name":"Center for Devices and Radiological Health","ror":"https://ror.org/007x9se63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070","https://openalex.org/I1335686170"]},{"id":"https://openalex.org/I1318287680","display_name":"Center for Biologics Evaluation and Research","ror":"https://ror.org/02nr3fr97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1318287680","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Marios A. Gavrielides","raw_affiliation_strings":["Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A"],"affiliations":[{"raw_affiliation_string":"Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","institution_ids":["https://openalex.org/I1335686170","https://openalex.org/I1320320070"]},{"raw_affiliation_string":"U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A","institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081026290","display_name":"H\u00e9la Masmoudi","orcid":null},"institutions":[{"id":"https://openalex.org/I193531525","display_name":"George Washington University","ror":"https://ror.org/00y4zzh67","country_code":"US","type":"education","lineage":["https://openalex.org/I193531525"]},{"id":"https://openalex.org/I1335686170","display_name":"Center for Devices and Radiological Health","ror":"https://ror.org/007x9se63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070","https://openalex.org/I1335686170"]},{"id":"https://openalex.org/I1318287680","display_name":"Center for Biologics Evaluation and Research","ror":"https://ror.org/02nr3fr97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1318287680","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hela Masmoudi","raw_affiliation_strings":["Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","Department of Electrical and Computer Engineering, The George Washington University, Washington, DC, U.S.A","U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A"],"affiliations":[{"raw_affiliation_string":"Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","institution_ids":["https://openalex.org/I1335686170","https://openalex.org/I1320320070"]},{"raw_affiliation_string":"Department of Electrical and Computer Engineering, The George Washington University, Washington, DC, U.S.A","institution_ids":["https://openalex.org/I193531525"]},{"raw_affiliation_string":"U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A","institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006593300","display_name":"Nicholas Petrick","orcid":"https://orcid.org/0000-0001-5167-8899"},"institutions":[{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1318287680","display_name":"Center for Biologics Evaluation and Research","ror":"https://ror.org/02nr3fr97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1318287680","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1335686170","display_name":"Center for Devices and Radiological Health","ror":"https://ror.org/007x9se63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070","https://openalex.org/I1335686170"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas Petrick","raw_affiliation_strings":["Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A"],"affiliations":[{"raw_affiliation_string":"Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","institution_ids":["https://openalex.org/I1335686170","https://openalex.org/I1320320070"]},{"raw_affiliation_string":"U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A","institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010335705","display_name":"Kyle J. Myers","orcid":"https://orcid.org/0000-0001-7394-4932"},"institutions":[{"id":"https://openalex.org/I1335686170","display_name":"Center for Devices and Radiological Health","ror":"https://ror.org/007x9se63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070","https://openalex.org/I1335686170"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]},{"id":"https://openalex.org/I1318287680","display_name":"Center for Biologics Evaluation and Research","ror":"https://ror.org/02nr3fr97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kyle J. Myers","raw_affiliation_strings":["Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A"],"affiliations":[{"raw_affiliation_string":"Center for Devices and Radiological Health CDRH Laboratory for the Assessment of Medical Imaging Systems, U.S Food and Drug Administration, National Institute of Biomedical Imaging and BioEngineering, Silver Spring, MD, USA","institution_ids":["https://openalex.org/I1335686170","https://openalex.org/I1320320070"]},{"raw_affiliation_string":"U.S Food and Drug Administration, Laboratory for the Assessment of Medical Imaging Systems, Silver Spring, MD, U.S.A","institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5011055578","display_name":"Stephen M. Hewitt","orcid":"https://orcid.org/0000-0001-8283-1788"},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen M. Hewitt","raw_affiliation_strings":["Tissue Array Research Program, National Institutes of Health, MD, U.S.A"],"affiliations":[{"raw_affiliation_string":"Tissue Array Research Program, National Institutes of Health, MD, U.S.A","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5039348485"],"corresponding_institution_ids":["https://openalex.org/I1318287680","https://openalex.org/I1320320070","https://openalex.org/I1335686170"],"apc_list":null,"apc_paid":null,"fwci":0.372,"has_fulltext":false,"cited_by_count":16,"citation_normalized_percentile":{"value":0.79885489,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":null,"issue":null,"first_page":"808","last_page":"811"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.9968000054359436,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"Immunohistochemistry","score":0.6917552351951599},{"id":"https://openalex.org/keywords/her2/neu","display_name":"HER2/neu","score":0.6584357023239136},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5463318824768066},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"Breast cancer","score":0.5324101448059082},{"id":"https://openalex.org/keywords/staining","display_name":"Staining","score":0.5079060196876526},{"id":"https://openalex.org/keywords/reproducibility","display_name":"Reproducibility","score":0.4666362404823303},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.46399256587028503},{"id":"https://openalex.org/keywords/automated-method","display_name":"Automated method","score":0.45951998233795166},{"id":"https://openalex.org/keywords/pathology","display_name":"Pathology","score":0.4461829364299774},{"id":"https://openalex.org/keywords/pattern-recognition","display_name":"Pattern recognition (psychology)","score":0.3334646224975586},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.2820154130458832},{"id":"https://openalex.org/keywords/cancer","display_name":"Cancer","score":0.2085629105567932},{"id":"https://openalex.org/keywords/internal-medicine","display_name":"Internal medicine","score":0.14042115211486816},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.1312394142150879},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.07539084553718567}],"concepts":[{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.6917552351951599},{"id":"https://openalex.org/C2776872082","wikidata":"https://www.wikidata.org/wiki/Q181874","display_name":"HER2/neu","level":4,"score":0.6584357023239136},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5463318824768066},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.5324101448059082},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.5079060196876526},{"id":"https://openalex.org/C9893847","wikidata":"https://www.wikidata.org/wiki/Q1425625","display_name":"Reproducibility","level":2,"score":0.4666362404823303},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.46399256587028503},{"id":"https://openalex.org/C3019060180","wikidata":"https://www.wikidata.org/wiki/Q184199","display_name":"Automated method","level":2,"score":0.45951998233795166},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4461829364299774},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.3334646224975586},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2820154130458832},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.2085629105567932},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.14042115211486816},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1312394142150879},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.07539084553718567}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1109/isbi.2008.4541119","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2008.4541119","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro","raw_type":"proceedings-article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.661.3620","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.661.3620","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://vision.lbl.gov/People/han/isbi2008/pdfs/0000808.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.7599999904632568,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W1561442812","https://openalex.org/W1983626331","https://openalex.org/W2091004209","https://openalex.org/W2109008952","https://openalex.org/W2141393790","https://openalex.org/W2160416036","https://openalex.org/W2161428561","https://openalex.org/W2288763477"],"related_works":["https://openalex.org/W2413717610","https://openalex.org/W1973270181","https://openalex.org/W2417696084","https://openalex.org/W2368782778","https://openalex.org/W2087830269","https://openalex.org/W1925251076","https://openalex.org/W3126923892","https://openalex.org/W2886899602","https://openalex.org/W2043413483","https://openalex.org/W2014909020"],"abstract_inverted_index":{"HER-2/neu":[0],"(HER2)":[1],"has":[2,16,27,119],"been":[3,17,28],"shown":[4],"to":[5,95,122],"be":[6,123],"a":[7,36,126],"valuable":[8],"biomarker":[9],"for":[10,52,73,129],"breast":[11],"cancer.":[12],"However,":[13],"inter-observer":[14],"variability":[15],"reported":[18],"in":[19],"the":[20,53,74,97,112,120,130,137],"evaluation":[21,33,132],"of":[22,41,56,70,76,133,139],"HER2":[23,42,57,80,134],"with":[24,111,136],"immunohistochemistry.":[25],"It":[26],"suggested":[29],"that":[30],"automated":[31,50,117],"computer-based":[32],"can":[34],"provide":[35],"consistent":[37],"and":[38,87],"objective":[39,138],"measure":[40],"expression.":[43],"In":[44],"this":[45],"manuscript,":[46],"we":[47],"present":[48],"an":[49,101],"method":[51,62,118],"quantitative":[54],"assessment":[55,104],"using":[58],"digital":[59],"microscopy.":[60],"The":[61,115],"employs":[63],"imaging":[64],"algorithms":[65],"on":[66],"whole":[67],"slide":[68,103],"images":[69],"tissue":[71],"specimens":[72],"extraction":[75],"two":[77],"features":[78,99],"describing":[79],"membrane":[81,84,88],"staining,":[82],"namely":[83],"staining":[85,89],"completeness":[86],"intensity.":[90],"A":[91],"classifier":[92],"was":[93],"trained":[94],"merge":[96],"extracted":[98],"into":[100],"overall":[102],"score.":[105],"Preliminary":[106],"results":[107],"showed":[108],"good":[109],"agreement":[110],"provided":[113],"truth.":[114],"developed":[116],"potential":[121],"used":[124],"as":[125],"computer":[127],"aid":[128],"immunohistochemical":[131],"expression":[135],"increasing":[140],"observer":[141],"reproducibility.":[142]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":2}],"updated_date":"2026-04-05T17:49:38.594831","created_date":"2025-10-10T00:00:00"}