{"id":"https://openalex.org/W1890677866","doi":"https://doi.org/10.1109/isbi.2002.1029397","title":"Automated determination of protein subcellular locations from 3D fluorescence microscope images","display_name":"Automated determination of protein subcellular locations from 3D fluorescence microscope images","publication_year":2003,"publication_date":"2003-06-25","ids":{"openalex":"https://openalex.org/W1890677866","doi":"https://doi.org/10.1109/isbi.2002.1029397","mag":"1890677866"},"language":"en","primary_location":{"id":"doi:10.1109/isbi.2002.1029397","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2002.1029397","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings IEEE International Symposium on Biomedical Imaging","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042233120","display_name":"Meel Velliste","orcid":null},"institutions":[{"id":"https://openalex.org/I74973139","display_name":"Carnegie Mellon University","ror":"https://ror.org/05x2bcf33","country_code":"US","type":"education","lineage":["https://openalex.org/I74973139"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"M. Velliste","raw_affiliation_strings":["Biomedical and Health Engineering Program and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA","[Dept. of Biol. Sci., Carnegie Mellon Univ., Pittsburgh, PA, USA]"],"affiliations":[{"raw_affiliation_string":"Biomedical and Health Engineering Program and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA","institution_ids":["https://openalex.org/I74973139"]},{"raw_affiliation_string":"[Dept. of Biol. Sci., Carnegie Mellon Univ., Pittsburgh, PA, USA]","institution_ids":["https://openalex.org/I74973139"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5061773929","display_name":"Robert F. Murphy","orcid":"https://orcid.org/0000-0003-0358-901X"},"institutions":[{"id":"https://openalex.org/I74973139","display_name":"Carnegie Mellon University","ror":"https://ror.org/05x2bcf33","country_code":"US","type":"education","lineage":["https://openalex.org/I74973139"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.F. Murphy","raw_affiliation_strings":["Biomedical and Health Engineering Program and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA","[Dept. of Biol. Sci., Carnegie Mellon Univ., Pittsburgh, PA, USA]"],"affiliations":[{"raw_affiliation_string":"Biomedical and Health Engineering Program and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA","institution_ids":["https://openalex.org/I74973139"]},{"raw_affiliation_string":"[Dept. of Biol. Sci., Carnegie Mellon Univ., Pittsburgh, PA, USA]","institution_ids":["https://openalex.org/I74973139"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5042233120"],"corresponding_institution_ids":["https://openalex.org/I74973139"],"apc_list":null,"apc_paid":null,"fwci":11.608,"has_fulltext":false,"cited_by_count":67,"citation_normalized_percentile":{"value":0.98402005,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":null,"issue":null,"first_page":"867","last_page":"870"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9977999925613403,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9914000034332275,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/subcellular-localization","display_name":"Subcellular localization","score":0.7987856864929199},{"id":"https://openalex.org/keywords/fluorescence-microscope","display_name":"Fluorescence microscope","score":0.6514321565628052},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.5904206037521362},{"id":"https://openalex.org/keywords/protein-subcellular-localization-prediction","display_name":"Protein subcellular localization prediction","score":0.5773506164550781},{"id":"https://openalex.org/keywords/golgi-apparatus","display_name":"Golgi apparatus","score":0.5665143728256226},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5409920811653137},{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.5087116360664368},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.5047463178634644},{"id":"https://openalex.org/keywords/microscope","display_name":"Microscope","score":0.4501327872276306},{"id":"https://openalex.org/keywords/pattern-recognition","display_name":"Pattern recognition (psychology)","score":0.44420546293258667},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.3485836386680603},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.2197985053062439},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.1971488893032074},{"id":"https://openalex.org/keywords/cell","display_name":"Cell","score":0.17920950055122375},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.14236891269683838},{"id":"https://openalex.org/keywords/cytoplasm","display_name":"Cytoplasm","score":0.11666175723075867},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.11317160725593567},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.11049598455429077}],"concepts":[{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.7987856864929199},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.6514321565628052},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.5904206037521362},{"id":"https://openalex.org/C140051345","wikidata":"https://www.wikidata.org/wiki/Q7251501","display_name":"Protein subcellular localization prediction","level":3,"score":0.5773506164550781},{"id":"https://openalex.org/C119062480","wikidata":"https://www.wikidata.org/wiki/Q83181","display_name":"Golgi apparatus","level":3,"score":0.5665143728256226},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5409920811653137},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5087116360664368},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.5047463178634644},{"id":"https://openalex.org/C67649825","wikidata":"https://www.wikidata.org/wiki/Q196538","display_name":"Microscope","level":2,"score":0.4501327872276306},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.44420546293258667},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.3485836386680603},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2197985053062439},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.1971488893032074},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.17920950055122375},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.14236891269683838},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.11666175723075867},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11317160725593567},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.11049598455429077},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1109/isbi.2002.1029397","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2002.1029397","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings IEEE International Symposium on Biomedical Imaging","raw_type":"proceedings-article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.88.6673","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.88.6673","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://murphylab.web.cmu.edu/publications/86-velliste2002.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1489703101","https://openalex.org/W1535305972","https://openalex.org/W1579543411","https://openalex.org/W1984643873","https://openalex.org/W2086216256","https://openalex.org/W2114869075","https://openalex.org/W2127208784","https://openalex.org/W2137632737","https://openalex.org/W2145752049"],"related_works":["https://openalex.org/W2351398203","https://openalex.org/W4205775722","https://openalex.org/W4366498163","https://openalex.org/W2402975874","https://openalex.org/W3215969703","https://openalex.org/W4389024241","https://openalex.org/W2384565783","https://openalex.org/W2406531951","https://openalex.org/W2099701744","https://openalex.org/W1530366416"],"abstract_inverted_index":{"Knowing":[0],"the":[1,28,32,44,83,86,90,94],"subcellular":[2,51,65],"location":[3,101],"of":[4,13,27,34,85,89,99,117],"a":[5,10],"protein":[6,100],"is":[7,43,80],"critical":[8],"to":[9],"full":[11],"understanding":[12],"its":[14],"function,":[15],"and":[16,53,96],"automated,":[17],"objective":[18],"methods":[19],"for":[20,31,49],"assigning":[21],"locations":[22],"are":[23,129],"needed":[24],"as":[25],"part":[26],"characterization":[29],"process":[30],"thousands":[33],"proteins":[35,126],"expressed":[36],"in":[37,67,103,107,111],"each":[38],"cell":[39,95],"type.":[40],"Fluorescence":[41],"microscopy":[42],"most":[45],"common":[46],"method":[47],"used":[48],"determining":[50],"location,":[52],"we":[54,73],"have":[55],"previously":[56],"described":[57],"automated":[58,115],"systems":[59],"that":[60,75,97],"can":[61],"recognize":[62],"all":[63],"major":[64],"structures":[66],"2D":[68,76,91],"fluorescence":[69],"microscope":[70],"images.":[71],"Here":[72],"show":[74],"pattern":[77],"recognition":[78],"accuracy":[79,109],"dependent":[81],"on":[82],"choice":[84],"vertical":[87],"position":[88],"slice":[92],"through":[93],"classification":[98],"patterns":[102,128],"3D":[104,118],"images":[105,119],"results":[106],"higher":[108],"than":[110],"2D.":[112],"In":[113],"particular,":[114],"analysis":[116],"provides":[120],"excellent":[121],"distinction":[122],"between":[123],"two":[124],"Golgi":[125],"whose":[127],"indistinguishable":[130],"by":[131],"visual":[132],"examination.":[133]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-04-04T16:13:02.066488","created_date":"2025-10-10T00:00:00"}