{"id":"https://openalex.org/W1613751815","doi":"https://doi.org/10.1109/isbi.2002.1029364","title":"Automated cryo-electron microscopy","display_name":"Automated cryo-electron microscopy","publication_year":2003,"publication_date":"2003-06-25","ids":{"openalex":"https://openalex.org/W1613751815","doi":"https://doi.org/10.1109/isbi.2002.1029364","mag":"1613751815"},"language":"en","primary_location":{"id":"doi:10.1109/isbi.2002.1029364","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2002.1029364","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings IEEE International Symposium on Biomedical Imaging","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5046815472","display_name":"Clinton S. Potter","orcid":"https://orcid.org/0000-0003-2394-0831"},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"C.S. Potter","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065340901","display_name":"D. Fellmann","orcid":null},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. Fellmann","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110693847","display_name":"Ronald A. Milligan","orcid":null},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.A. Milligan","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112014552","display_name":"J Pulokas","orcid":null},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Pulokas","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039894080","display_name":"Christian Suloway","orcid":"https://orcid.org/0000-0002-6710-503X"},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Suloway","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086646606","display_name":"Yu Zhu","orcid":"https://orcid.org/0000-0002-0339-8283"},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y. Zhu","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034550631","display_name":"A. Carragher","orcid":null},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A. Carragher","raw_affiliation_strings":["Scripps Research Institute, La Jolla, CA, USA","The Scripps Research Institute, La Jolla, CA, USA"],"affiliations":[{"raw_affiliation_string":"Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"The Scripps Research Institute, La Jolla, CA, USA","institution_ids":["https://openalex.org/I123431417"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5046815472"],"corresponding_institution_ids":["https://openalex.org/I123431417"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0139485,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"7","issue":null,"first_page":"741","last_page":"744"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.9980999827384949,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cryo-electron-microscopy","display_name":"Cryo-electron microscopy","score":0.6283394694328308},{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.6037904024124146},{"id":"https://openalex.org/keywords/electron-microscope","display_name":"Electron microscope","score":0.5763298273086548},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.5577554106712341},{"id":"https://openalex.org/keywords/tobacco-mosaic-virus","display_name":"Tobacco mosaic virus","score":0.5518112182617188},{"id":"https://openalex.org/keywords/image-processing","display_name":"Image processing","score":0.447032630443573},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.3499823808670044},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.34546834230422974},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.34002962708473206},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.2944146394729614},{"id":"https://openalex.org/keywords/image","display_name":"Image (mathematics)","score":0.22398266196250916},{"id":"https://openalex.org/keywords/nuclear-magnetic-resonance","display_name":"Nuclear magnetic resonance","score":0.20426994562149048},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.18571433424949646},{"id":"https://openalex.org/keywords/virus","display_name":"Virus","score":0.18309512734413147},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.16730192303657532},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.15822923183441162},{"id":"https://openalex.org/keywords/virology","display_name":"Virology","score":0.11921676993370056}],"concepts":[{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.6283394694328308},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.6037904024124146},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.5763298273086548},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5577554106712341},{"id":"https://openalex.org/C144707691","wikidata":"https://www.wikidata.org/wiki/Q332874","display_name":"Tobacco mosaic virus","level":3,"score":0.5518112182617188},{"id":"https://openalex.org/C9417928","wikidata":"https://www.wikidata.org/wiki/Q1070689","display_name":"Image processing","level":3,"score":0.447032630443573},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.3499823808670044},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.34546834230422974},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.34002962708473206},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2944146394729614},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.22398266196250916},{"id":"https://openalex.org/C46141821","wikidata":"https://www.wikidata.org/wiki/Q209402","display_name":"Nuclear magnetic resonance","level":1,"score":0.20426994562149048},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.18571433424949646},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.18309512734413147},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.16730192303657532},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.15822923183441162},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.11921676993370056}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/isbi.2002.1029364","is_oa":false,"landing_page_url":"https://doi.org/10.1109/isbi.2002.1029364","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings IEEE International Symposium on Biomedical Imaging","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6100000143051147,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1677689941","https://openalex.org/W1947778485","https://openalex.org/W1994345832","https://openalex.org/W2025175582","https://openalex.org/W2037033695","https://openalex.org/W2044074387","https://openalex.org/W2069408618","https://openalex.org/W2069915544","https://openalex.org/W2072575434","https://openalex.org/W2094869942","https://openalex.org/W2095057795","https://openalex.org/W2095384140","https://openalex.org/W2148802050","https://openalex.org/W2149476505","https://openalex.org/W2149705812","https://openalex.org/W2167329917","https://openalex.org/W2423227224"],"related_works":["https://openalex.org/W1995785876","https://openalex.org/W1971080731","https://openalex.org/W2106173226","https://openalex.org/W3169882331","https://openalex.org/W1506992858","https://openalex.org/W3111994488","https://openalex.org/W168865677","https://openalex.org/W2072424450","https://openalex.org/W2567303839","https://openalex.org/W1520158892"],"abstract_inverted_index":{"Cryo-electron":[0],"microscopy":[1,42],"is":[2,47],"widely":[3],"viewed":[4,24],"as":[5,25,91],"a":[6,92],"uniquely":[7],"powerful":[8],"method":[9],"for":[10,65],"the":[11,38],"study":[12,31],"of":[13,40,75,94],"membrane":[14],"proteins":[15],"and":[16,50,63,81],"large":[17],"macromolecular":[18],"complexes":[19],"-":[20],"subjects":[21],"that":[22],"are":[23],"extremely":[26],"challenging":[27],"or":[28,34],"impossible":[29],"to":[30,59],"using":[32],"x-ray":[33],"NMR":[35],"methods.":[36],"Although":[37],"methodology":[39],"molecular":[41],"has":[43,55],"enormous":[44],"potential,":[45],"it":[46],"time":[48],"consuming":[49],"labor":[51],"intensive.":[52],"Our":[53],"group":[54],"done":[56],"extensive":[57],"work":[58],"automate":[60],"image":[61],"acquisition":[62],"processing":[64],"cryo-EM.":[66],"In":[67],"this":[68],"paper":[69],"we":[70,85],"will":[71],"provide":[72],"an":[73],"overview":[74],"our":[76],"automated":[77],"system,":[78],"called":[79],"Leginon,":[80],"present":[82],"results":[83],"where":[84],"used":[86],"tobacco":[87],"mosaic":[88],"virus":[89],"(TMV)":[90],"proof":[93],"concept.":[95]},"counts_by_year":[],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}