{"id":"https://openalex.org/W4410428647","doi":"https://doi.org/10.1109/icst62759.2024.10992244","title":"Exploring the Enhancement Effect of Dithiothreitol on CRISPR/Cas12b Biosensing System","display_name":"Exploring the Enhancement Effect of Dithiothreitol on CRISPR/Cas12b Biosensing System","publication_year":2024,"publication_date":"2024-12-09","ids":{"openalex":"https://openalex.org/W4410428647","doi":"https://doi.org/10.1109/icst62759.2024.10992244"},"language":"en","primary_location":{"id":"doi:10.1109/icst62759.2024.10992244","is_oa":false,"landing_page_url":"https://doi.org/10.1109/icst62759.2024.10992244","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2024 17th International Conference on Sensing Technology (ICST)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://unsworks.unsw.edu.au/bitstreams/b6d503d0-8daa-468e-968b-95fb013a33b9/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113331720","display_name":"Ruier Xue","orcid":null},"institutions":[{"id":"https://openalex.org/I31746571","display_name":"UNSW Sydney","ror":"https://ror.org/03r8z3t63","country_code":"AU","type":"education","lineage":["https://openalex.org/I31746571"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Ruier Xue","raw_affiliation_strings":["Graduate School of Biomedical Engineering, UNSW Sydney,Sydney,Australia"],"affiliations":[{"raw_affiliation_string":"Graduate School of Biomedical Engineering, UNSW Sydney,Sydney,Australia","institution_ids":["https://openalex.org/I31746571"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016646734","display_name":"Fei Deng","orcid":"https://orcid.org/0000-0002-5606-0278"},"institutions":[{"id":"https://openalex.org/I31746571","display_name":"UNSW Sydney","ror":"https://ror.org/03r8z3t63","country_code":"AU","type":"education","lineage":["https://openalex.org/I31746571"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Fei Deng","raw_affiliation_strings":["Graduate School of Biomedical Engineering, UNSW Sydney,Sydney,Australia"],"affiliations":[{"raw_affiliation_string":"Graduate School of Biomedical Engineering, UNSW Sydney,Sydney,Australia","institution_ids":["https://openalex.org/I31746571"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5113331720"],"corresponding_institution_ids":["https://openalex.org/I31746571"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.23016581,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"4"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.3855000138282776,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.3855000138282776,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.3797999918460846,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.7806113958358765},{"id":"https://openalex.org/keywords/biosensor","display_name":"Biosensor","score":0.7790202498435974},{"id":"https://openalex.org/keywords/dithiothreitol","display_name":"Dithiothreitol","score":0.711786150932312},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.46618884801864624},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.45239558815956116},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.29789143800735474},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.28894928097724915},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.07940617203712463}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7806113958358765},{"id":"https://openalex.org/C160756335","wikidata":"https://www.wikidata.org/wiki/Q669391","display_name":"Biosensor","level":2,"score":0.7790202498435974},{"id":"https://openalex.org/C2777824588","wikidata":"https://www.wikidata.org/wiki/Q414783","display_name":"Dithiothreitol","level":3,"score":0.711786150932312},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.46618884801864624},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.45239558815956116},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.29789143800735474},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.28894928097724915},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07940617203712463},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1109/icst62759.2024.10992244","is_oa":false,"landing_page_url":"https://doi.org/10.1109/icst62759.2024.10992244","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2024 17th International Conference on Sensing Technology (ICST)","raw_type":"proceedings-article"},{"id":"pmh:oai:unsworks.library.unsw.edu.au:1959.4/105374","is_oa":true,"landing_page_url":"http://hdl.handle.net/1959.4/105374","pdf_url":"https://unsworks.unsw.edu.au/bitstreams/b6d503d0-8daa-468e-968b-95fb013a33b9/download","source":{"id":"https://openalex.org/S4306401737","display_name":"UNSWorks (University of New South Wales, Sydney, Australia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I40053085","host_organization_name":"Australian Defence Force Academy","host_organization_lineage":["https://openalex.org/I40053085"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"2024 17th International Conference on Sensing Technology (ICST), AUSTRALIA, Sydney, 2024-12-09 - 2024-12-11","raw_type":"http://purl.org/coar/resource_type/c_5794"}],"best_oa_location":{"id":"pmh:oai:unsworks.library.unsw.edu.au:1959.4/105374","is_oa":true,"landing_page_url":"http://hdl.handle.net/1959.4/105374","pdf_url":"https://unsworks.unsw.edu.au/bitstreams/b6d503d0-8daa-468e-968b-95fb013a33b9/download","source":{"id":"https://openalex.org/S4306401737","display_name":"UNSWorks (University of New South Wales, Sydney, Australia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I40053085","host_organization_name":"Australian Defence Force Academy","host_organization_lineage":["https://openalex.org/I40053085"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"2024 17th International Conference on Sensing Technology (ICST), AUSTRALIA, Sydney, 2024-12-09 - 2024-12-11","raw_type":"http://purl.org/coar/resource_type/c_5794"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4410428647.pdf","grobid_xml":"https://content.openalex.org/works/W4410428647.grobid-xml"},"referenced_works_count":14,"referenced_works":["https://openalex.org/W2020636366","https://openalex.org/W2563531760","https://openalex.org/W2921430890","https://openalex.org/W2953627903","https://openalex.org/W3000481248","https://openalex.org/W3135497523","https://openalex.org/W3162028474","https://openalex.org/W3182002460","https://openalex.org/W4220656942","https://openalex.org/W4281661400","https://openalex.org/W4297990592","https://openalex.org/W4362470284","https://openalex.org/W4389576855","https://openalex.org/W4390786904"],"related_works":["https://openalex.org/W4391375266","https://openalex.org/W2899084033","https://openalex.org/W2748952813","https://openalex.org/W2017595667","https://openalex.org/W2101874883","https://openalex.org/W655656535","https://openalex.org/W2057291091","https://openalex.org/W2074021765","https://openalex.org/W2199494561","https://openalex.org/W2049117872"],"abstract_inverted_index":{"Biosensors":[0],"are":[1],"essential":[2],"for":[3,107,122],"early":[4],"diagnoses":[5],"and":[6,13,23,27,104,125],"effective":[7],"disease":[8],"management":[9],"by":[10,91],"enabling":[11],"rapid":[12,105],"accurate":[14,124],"detection":[15,98],"of":[16,51,58,73],"specific":[17],"biomarkers.":[18],"However,":[19],"achieving":[20,92],"high":[21],"sensitivity":[22],"accuracy":[24],"with":[25,31],"simplicity":[26],"accessibility":[28],"remains":[29],"challenging":[30],"current":[32],"CRISPR/Cas":[33],"technologies,":[34],"hindering":[35],"their":[36],"clinical":[37],"diagnostic":[38],"applications.":[39],"To":[40],"address":[41],"this":[42,44],"issue,":[43],"study":[45],"investigates":[46],"the":[47,55],"chemical":[48],"enhancement":[49],"effect":[50],"dithiothreitol":[52],"(DTT)":[53],"on":[54,80],"trans-cleavage":[56,78],"activity":[57],"Cas12b":[59],"nucleases.":[60],"Our":[61],"optimised":[62],"biosensing":[63,116],"system":[64,86],"demonstrated":[65],"elevated":[66],"fluorescence":[67],"intensity":[68],"levels":[69],"after":[70],"30":[71],"minutes":[72],"thermal":[74],"incubation,":[75],"indicating":[76],"enhanced":[77],"efficiency":[79],"fluorescent-quench":[81],"reporters.":[82],"The":[83],"DTT-assisted":[84],"CRISPR/Cas12b":[85],"significantly":[87],"enhances":[88],"sensor":[89],"performance":[90],"a":[93,101,119],"tenfold":[94],"improvement":[95],"in":[96,114],"its":[97],"limit,":[99],"offering":[100],"precise,":[102],"sensitive":[103],"tool":[106],"detecting":[108],"nucleic":[109],"acid":[110],"targets.":[111],"This":[112],"advancement":[113],"CRISPR/Cas-based":[115],"technology":[117],"provides":[118],"promising":[120],"approach":[121],"more":[123],"efficient":[126],"point-of-care":[127],"diagnoses.":[128]},"counts_by_year":[],"updated_date":"2026-03-10T16:38:18.471706","created_date":"2025-10-10T00:00:00"}