{"id":"https://openalex.org/W2024344214","doi":"https://doi.org/10.1109/ias.2011.6074267","title":"A novel gene transformation technique using water-in-oil droplet in an electrostatic field","display_name":"A novel gene transformation technique using water-in-oil droplet in an electrostatic field","publication_year":2011,"publication_date":"2011-10-01","ids":{"openalex":"https://openalex.org/W2024344214","doi":"https://doi.org/10.1109/ias.2011.6074267","mag":"2024344214"},"language":"en","primary_location":{"id":"doi:10.1109/ias.2011.6074267","is_oa":false,"landing_page_url":"https://doi.org/10.1109/ias.2011.6074267","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 IEEE Industry Applications Society Annual Meeting","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079124410","display_name":"Atsushi Asada","orcid":null},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Atsushi Asada","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074222770","display_name":"Hironori Aoki","orcid":"https://orcid.org/0000-0002-0070-4900"},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hironori Aoki","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071258206","display_name":"Hirofumi Kurita","orcid":"https://orcid.org/0000-0002-2538-6590"},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hirofumi Kurita","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004468102","display_name":"Hachiro Yasuda","orcid":"https://orcid.org/0000-0001-9937-8166"},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hachiro Yasuda","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024658554","display_name":"Kazunori Takashima","orcid":"https://orcid.org/0000-0003-4863-3029"},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazunori Takashima","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056217295","display_name":"Akira Mizuno","orcid":"https://orcid.org/0000-0002-6414-6143"},"institutions":[{"id":"https://openalex.org/I136259955","display_name":"Toyohashi University of Technology","ror":"https://ror.org/04ezg6d83","country_code":"JP","type":"education","lineage":["https://openalex.org/I136259955"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akira Mizuno","raw_affiliation_strings":["Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan"],"affiliations":[{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, Toyohashi, Aichi, Japan","institution_ids":["https://openalex.org/I136259955"]},{"raw_affiliation_string":"Department of Environmental and Life Sciences, Graduate School of Engineering, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku-cho, Aichi 441-8580, Japan","institution_ids":["https://openalex.org/I136259955"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5079124410"],"corresponding_institution_ids":["https://openalex.org/I136259955"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.09950416,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"4"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12776","display_name":"Electrohydrodynamics and Fluid Dynamics","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/2208","display_name":"Electrical and Electronic Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.9962000250816345,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/electric-field","display_name":"Electric field","score":0.7420713901519775},{"id":"https://openalex.org/keywords/electrode","display_name":"Electrode","score":0.6472821235656738},{"id":"https://openalex.org/keywords/transformation","display_name":"Transformation (genetics)","score":0.6370261311531067},{"id":"https://openalex.org/keywords/coulombs-law","display_name":"Coulomb's law","score":0.6308801174163818},{"id":"https://openalex.org/keywords/electrostatics","display_name":"Electrostatics","score":0.6163722276687622},{"id":"https://openalex.org/keywords/voltage","display_name":"Voltage","score":0.5549910664558411},{"id":"https://openalex.org/keywords/coulomb","display_name":"Coulomb","score":0.5076097249984741},{"id":"https://openalex.org/keywords/transfection","display_name":"Transfection","score":0.45382723212242126},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.43115073442459106},{"id":"https://openalex.org/keywords/gene-delivery","display_name":"Gene delivery","score":0.4237546920776367},{"id":"https://openalex.org/keywords/nanotechnology","display_name":"Nanotechnology","score":0.4209240674972534},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.34663981199264526},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.2318483591079712},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.19578877091407776},{"id":"https://openalex.org/keywords/electrical-engineering","display_name":"Electrical engineering","score":0.1512024998664856},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.10172149538993835}],"concepts":[{"id":"https://openalex.org/C60799052","wikidata":"https://www.wikidata.org/wiki/Q46221","display_name":"Electric field","level":2,"score":0.7420713901519775},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.6472821235656738},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.6370261311531067},{"id":"https://openalex.org/C193874036","wikidata":"https://www.wikidata.org/wiki/Q83152","display_name":"Coulomb's law","level":4,"score":0.6308801174163818},{"id":"https://openalex.org/C117626034","wikidata":"https://www.wikidata.org/wiki/Q26336","display_name":"Electrostatics","level":2,"score":0.6163722276687622},{"id":"https://openalex.org/C165801399","wikidata":"https://www.wikidata.org/wiki/Q25428","display_name":"Voltage","level":2,"score":0.5549910664558411},{"id":"https://openalex.org/C9342510","wikidata":"https://www.wikidata.org/wiki/Q25406","display_name":"Coulomb","level":3,"score":0.5076097249984741},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.45382723212242126},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.43115073442459106},{"id":"https://openalex.org/C135983454","wikidata":"https://www.wikidata.org/wiki/Q2790163","display_name":"Gene delivery","level":4,"score":0.4237546920776367},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4209240674972534},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34663981199264526},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2318483591079712},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.19578877091407776},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.1512024998664856},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.10172149538993835},{"id":"https://openalex.org/C147120987","wikidata":"https://www.wikidata.org/wiki/Q2225","display_name":"Electron","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/ias.2011.6074267","is_oa":false,"landing_page_url":"https://doi.org/10.1109/ias.2011.6074267","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 IEEE Industry Applications Society Annual Meeting","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation","score":0.8299999833106995}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W1968326460","https://openalex.org/W1975960938","https://openalex.org/W2030711123","https://openalex.org/W2043700440","https://openalex.org/W2067358773"],"related_works":["https://openalex.org/W4281707391","https://openalex.org/W2523943737","https://openalex.org/W2976906301","https://openalex.org/W2904616103","https://openalex.org/W2086182564","https://openalex.org/W2052288565","https://openalex.org/W201818518","https://openalex.org/W2033029089","https://openalex.org/W1840006687","https://openalex.org/W3204150836"],"abstract_inverted_index":{"Droplet-based":[0],"micro":[1],"total":[2],"analysis":[3],"systems":[4],"(\u03bcTAS)":[5],"have":[6],"been":[7],"investigated":[8],"by":[9,42,79,116],"many":[10],"researchers":[11],"and":[12,65,92,100],"used":[13],"in":[14,52,56,149],"bioengineering.":[15,151],"In":[16,68,123],"these":[17],"systems,":[18],"individual":[19],"water-in-oil":[20],"(W/O)":[21],"droplets":[22,55],"perform":[23],"as":[24],"micro-reactors.":[25],"To":[26],"manipulate":[27],"W/O":[28,54],"droplets,":[29],"an":[30],"electrostatic":[31,118],"field":[32,48],"is":[33,142],"one":[34],"of":[35,133],"the":[36,57,81,98,108,113,117],"most":[37],"useful":[38],"tools.":[39],"For":[40],"example,":[41],"applying":[43],"a":[44,106,146],"direct":[45],"current":[46],"electric":[47],"between":[49,97],"electrodes":[50,58,99],"soaked":[51],"oil,":[53],"spacings":[59],"reciprocate":[60],"due":[61],"to":[62,73,144],"dielectrophoretic":[63],"force":[64],"Coulomb":[66],"force.":[67],"this":[69,124],"study,":[70],"gene":[71,114,139],"transformation":[72,115],"Escherichia":[74],"coli":[75,91],"(E.":[76],"coli)":[77],"cells":[78],"using":[80],"droplet":[82,88,119],"reciprocation":[83],"was":[84,95,103,121],"carried":[85],"out.":[86],"A":[87],"containing":[89],"E.":[90],"plasmid":[93],"DNA":[94],"located":[96],"DC":[101],"voltage":[102],"applied.":[104],"As":[105],"result,":[107],"transformants":[109],"were":[110],"obtained.":[111],"Therefore":[112],"manipulation":[120],"confirmed.":[122],"method,":[125],"sample":[126],"volume":[127],"can":[128],"be":[129,145],"smaller":[130],"than":[131],"that":[132],"conventional":[134],"method.":[135],"Therefore,":[136],"our":[137],"novel":[138],"transfection":[140],"method":[141],"expected":[143],"new":[147],"tool":[148],"miniaturized":[150]},"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}
