{"id":"https://openalex.org/W4389537687","doi":"https://doi.org/10.1109/embc40787.2023.10340148","title":"Quantitative Analysis of Differentiation Activity for Mouse Embryonic Stem Cells by Deep Learning for Cell Center Detection using Three-Dimensional Confocal Fluorescence Microscopy Images","display_name":"Quantitative Analysis of Differentiation Activity for Mouse Embryonic Stem Cells by Deep Learning for Cell Center Detection using Three-Dimensional Confocal Fluorescence Microscopy Images","publication_year":2023,"publication_date":"2023-07-24","ids":{"openalex":"https://openalex.org/W4389537687","doi":"https://doi.org/10.1109/embc40787.2023.10340148","pmid":"https://pubmed.ncbi.nlm.nih.gov/38083144"},"language":"en","primary_location":{"id":"doi:10.1109/embc40787.2023.10340148","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1109/embc40787.2023.10340148","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2023 45th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038121796","display_name":"Slo\u2010Li Chu","orcid":"https://orcid.org/0000-0003-3999-7293"},"institutions":[{"id":"https://openalex.org/I151221077","display_name":"Chung Yuan Christian University","ror":"https://ror.org/02w8ws377","country_code":"TW","type":"education","lineage":["https://openalex.org/I151221077"]}],"countries":["TW"],"is_corresponding":true,"raw_author_name":"Slo-Li Chu","raw_affiliation_strings":["Chung-Yuan Christian University,Department of Information and Computer Engineering,Taoyuan,Taiwan,32023"],"affiliations":[{"raw_affiliation_string":"Chung-Yuan Christian University,Department of Information and Computer Engineering,Taoyuan,Taiwan,32023","institution_ids":["https://openalex.org/I151221077"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014175129","display_name":"Hideo Yokota","orcid":"https://orcid.org/0000-0003-1395-309X"},"institutions":[{"id":"https://openalex.org/I4210165842","display_name":"RIKEN Center for Advanced Photonics","ror":"https://ror.org/05vmjks78","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210110652","https://openalex.org/I4210165842"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hideo Yokota","raw_affiliation_strings":["RIKEN,Center for Advanced Photonics,Saitama,Japan,351-0198"],"affiliations":[{"raw_affiliation_string":"RIKEN,Center for Advanced Photonics,Saitama,Japan,351-0198","institution_ids":["https://openalex.org/I4210165842"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088485562","display_name":"Hao-Lun Hsieh","orcid":null},"institutions":[{"id":"https://openalex.org/I151221077","display_name":"Chung Yuan Christian University","ror":"https://ror.org/02w8ws377","country_code":"TW","type":"education","lineage":["https://openalex.org/I151221077"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Hao-Lun Hsieh","raw_affiliation_strings":["Chung-Yuan Christian University,Master Program in Department of Information and Computer Engineering","Master Program in Department of Information and Computer Engineering, Chung-Yuan Christian University"],"affiliations":[{"raw_affiliation_string":"Chung-Yuan Christian University,Master Program in Department of Information and Computer Engineering","institution_ids":["https://openalex.org/I151221077"]},{"raw_affiliation_string":"Master Program in Department of Information and Computer Engineering, Chung-Yuan Christian University","institution_ids":["https://openalex.org/I151221077"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026112234","display_name":"Kuniya Abe","orcid":"https://orcid.org/0000-0002-6912-8526"},"institutions":[{"id":"https://openalex.org/I4210096362","display_name":"RIKEN BioResource Research Center","ror":"https://ror.org/00s05em53","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210096362","https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kuniya Abe","raw_affiliation_strings":["RIKEN,BioResource Research Center,Ibaraki,Japan,305-0074"],"affiliations":[{"raw_affiliation_string":"RIKEN,BioResource Research Center,Ibaraki,Japan,305-0074","institution_ids":["https://openalex.org/I4210096362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001012166","display_name":"Dooseon Cho","orcid":"https://orcid.org/0000-0002-0067-5804"},"institutions":[{"id":"https://openalex.org/I4210096362","display_name":"RIKEN BioResource Research Center","ror":"https://ror.org/00s05em53","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210096362","https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Dooseon Cho","raw_affiliation_strings":["RIKEN,BioResource Research Center,Ibaraki,Japan,305-0074"],"affiliations":[{"raw_affiliation_string":"RIKEN,BioResource Research Center,Ibaraki,Japan,305-0074","institution_ids":["https://openalex.org/I4210096362"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109115083","display_name":"Ming-Dar Tsai","orcid":null},"institutions":[{"id":"https://openalex.org/I151221077","display_name":"Chung Yuan Christian University","ror":"https://ror.org/02w8ws377","country_code":"TW","type":"education","lineage":["https://openalex.org/I151221077"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Ming-Dar Tsai","raw_affiliation_strings":["Chung-Yuan Christian University,Department of Information and Computer Engineering,Taoyuan,Taiwan,32023"],"affiliations":[{"raw_affiliation_string":"Chung-Yuan Christian University,Department of Information and Computer Engineering,Taoyuan,Taiwan,32023","institution_ids":["https://openalex.org/I151221077"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5038121796"],"corresponding_institution_ids":["https://openalex.org/I151221077"],"apc_list":null,"apc_paid":null,"fwci":0.3661,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.78340581,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"2023","issue":null,"first_page":"1","last_page":"4"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13114","display_name":"Image Processing Techniques and Applications","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/2214","display_name":"Media Technology"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/confocal","display_name":"Confocal","score":0.7089521884918213},{"id":"https://openalex.org/keywords/confocal-microscopy","display_name":"Confocal microscopy","score":0.6423951983451843},{"id":"https://openalex.org/keywords/embryonic-stem-cell","display_name":"Embryonic stem cell","score":0.6228030323982239},{"id":"https://openalex.org/keywords/segmentation","display_name":"Segmentation","score":0.5220080018043518},{"id":"https://openalex.org/keywords/population","display_name":"Population","score":0.49190640449523926},{"id":"https://openalex.org/keywords/microscopy","display_name":"Microscopy","score":0.4542085528373718},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.43820708990097046},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.43539321422576904},{"id":"https://openalex.org/keywords/image-segmentation","display_name":"Image segmentation","score":0.4288588762283325},{"id":"https://openalex.org/keywords/center","display_name":"Center (category theory)","score":0.4259512424468994},{"id":"https://openalex.org/keywords/fluorescence-microscope","display_name":"Fluorescence microscope","score":0.41851240396499634},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.411584734916687},{"id":"https://openalex.org/keywords/fluorescence","display_name":"Fluorescence","score":0.37092721462249756},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.21999090909957886},{"id":"https://openalex.org/keywords/optics","display_name":"Optics","score":0.21286731958389282},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.20542758703231812},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.19481846690177917},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.1942976415157318},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.08732986450195312}],"concepts":[{"id":"https://openalex.org/C136009344","wikidata":"https://www.wikidata.org/wiki/Q336201","display_name":"Confocal","level":2,"score":0.7089521884918213},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.6423951983451843},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.6228030323982239},{"id":"https://openalex.org/C89600930","wikidata":"https://www.wikidata.org/wiki/Q1423946","display_name":"Segmentation","level":2,"score":0.5220080018043518},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.49190640449523926},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4542085528373718},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.43820708990097046},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.43539321422576904},{"id":"https://openalex.org/C124504099","wikidata":"https://www.wikidata.org/wiki/Q56933","display_name":"Image segmentation","level":3,"score":0.4288588762283325},{"id":"https://openalex.org/C2779463800","wikidata":"https://www.wikidata.org/wiki/Q5062222","display_name":"Center (category theory)","level":2,"score":0.4259512424468994},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.41851240396499634},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.411584734916687},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.37092721462249756},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.21999090909957886},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.21286731958389282},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20542758703231812},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.19481846690177917},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1942976415157318},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08732986450195312},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000066450","descriptor_name":"Mouse Embryonic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000066450","descriptor_name":"Mouse Embryonic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000066450","descriptor_name":"Mouse Embryonic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000066450","descriptor_name":"Mouse Embryonic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000066450","descriptor_name":"Mouse Embryonic Stem Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000077321","descriptor_name":"Deep Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000077321","descriptor_name":"Deep Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000077321","descriptor_name":"Deep Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000077321","descriptor_name":"Deep Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000077321","descriptor_name":"Deep Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021621","descriptor_name":"Imaging, Three-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021621","descriptor_name":"Imaging, Three-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021621","descriptor_name":"Imaging, Three-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021621","descriptor_name":"Imaging, Three-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D021621","descriptor_name":"Imaging, Three-Dimensional","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1109/embc40787.2023.10340148","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1109/embc40787.2023.10340148","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2023 45th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC)","raw_type":"proceedings-article"},{"id":"pmid:38083144","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38083144","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320309618","display_name":"Ministry of Science and Technology","ror":"https://ror.org/02b207r52"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":7,"referenced_works":["https://openalex.org/W2109798202","https://openalex.org/W2900936384","https://openalex.org/W3045763514","https://openalex.org/W3093569261","https://openalex.org/W4200208483","https://openalex.org/W4200337482","https://openalex.org/W4295434144"],"related_works":["https://openalex.org/W2726111829","https://openalex.org/W2008049857","https://openalex.org/W2056668469","https://openalex.org/W4250585504","https://openalex.org/W2105513520","https://openalex.org/W1978803831","https://openalex.org/W2999303294","https://openalex.org/W2580002495","https://openalex.org/W82035557","https://openalex.org/W1539232953"],"abstract_inverted_index":{"Accurate":[0],"single":[1,11,28,55,89],"cell":[2,12,32,40,56,68,72,90,135],"segmentation":[3,57],"provides":[4],"means":[5],"to":[6,23,61,83,101,119],"monitor":[7,36],"the":[8,39,50,63,74,102,121,140],"behavior":[9],"of":[10,16,27,38,44,49,66,87,97],"within":[13],"a":[14,79],"population":[15],"cells.":[17],"Time-lapse":[18],"fluorescence":[19],"images":[20,100],"are":[21],"used":[22,60],"reveal":[24],"heterogeneous":[25],"nature":[26],"mouse":[29],"embryonic":[30],"stem":[31],"(ESC)":[33],"colony":[34],"and":[35,46,69,138,145],"fluctuations":[37],"states.":[41],"Automatic":[42],"quantification":[43,141],"speed":[45],"status":[47,146],"shifts":[48],"ESCs":[51],"depends":[52],"on":[53],"accurate":[54],"that":[58,107,128],"is":[59,104],"calculate":[62],"3D":[64,81,92,99],"center":[65,86,109,122,136],"every":[67],"track":[70],"this":[71],"for":[73,134,142],"quantification.":[75],"This":[76,125],"study":[77,126],"proposes":[78],"new":[80],"U-net":[82,103],"accurately":[84],"detect":[85],"each":[88],"in":[91],"confocal":[93],"images.":[94],"The":[95],"dimension":[96],"input":[98],"flexible":[105],"so":[106],"multiple":[108],"detections":[110],"from":[111],"different":[112],"image":[113],"directions":[114],"can":[115,131],"be":[116],"implemented":[117],"simultaneously":[118],"improve":[120,132],"detection":[123,137],"accuracy.":[124],"showed":[127],"our":[129],"method":[130],"accuracy":[133],"thus":[139],"ESC":[143],"speeds":[144],"shifts.":[147]},"counts_by_year":[{"year":2024,"cited_by_count":1}],"updated_date":"2025-12-25T23:11:45.687758","created_date":"2025-10-10T00:00:00"}