{"id":"https://openalex.org/W2536882482","doi":"https://doi.org/10.1109/embc.2016.7590984","title":"Modeling of coupled differential equations for cellular chemical signaling pathways: Implications for assay protocols utilized in cellular engineering","display_name":"Modeling of coupled differential equations for cellular chemical signaling pathways: Implications for assay protocols utilized in cellular engineering","publication_year":2016,"publication_date":"2016-08-01","ids":{"openalex":"https://openalex.org/W2536882482","doi":"https://doi.org/10.1109/embc.2016.7590984","mag":"2536882482","pmid":"https://pubmed.ncbi.nlm.nih.gov/28268601"},"language":"en","primary_location":{"id":"doi:10.1109/embc.2016.7590984","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2016.7590984","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013311247","display_name":"George D. O\u2019Clock","orcid":null},"institutions":[{"id":"https://openalex.org/I4210135252","display_name":"University of Minnesota Medical Center","ror":"https://ror.org/03e1ayz78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1309104334","https://openalex.org/I4210135252"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"George D. O'Clock","raw_affiliation_strings":["Biomedical Engineering Department, University of Minnesota Medical School, Minneapolis, MN, USA"],"affiliations":[{"raw_affiliation_string":"Biomedical Engineering Department, University of Minnesota Medical School, Minneapolis, MN, USA","institution_ids":["https://openalex.org/I4210135252"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013311247"],"corresponding_institution_ids":["https://openalex.org/I4210135252"],"apc_list":null,"apc_paid":null,"fwci":0.1667,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.63980461,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"2016","issue":null,"first_page":"1459","last_page":"1462"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9954000115394592,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9696000218391418,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/signal-transduction","display_name":"Signal transduction","score":0.5353990197181702},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.4869919717311859},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.46052178740501404},{"id":"https://openalex.org/keywords/control-theory","display_name":"Control theory (sociology)","score":0.3620607852935791},{"id":"https://openalex.org/keywords/electronic-engineering","display_name":"Electronic engineering","score":0.3221718668937683},{"id":"https://openalex.org/keywords/cell-biology","display_name":"Cell biology","score":0.29839491844177246},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.21471071243286133},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.18103983998298645},{"id":"https://openalex.org/keywords/control","display_name":"Control (management)","score":0.15435945987701416}],"concepts":[{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.5353990197181702},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.4869919717311859},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.46052178740501404},{"id":"https://openalex.org/C47446073","wikidata":"https://www.wikidata.org/wiki/Q5165890","display_name":"Control theory (sociology)","level":3,"score":0.3620607852935791},{"id":"https://openalex.org/C24326235","wikidata":"https://www.wikidata.org/wiki/Q126095","display_name":"Electronic engineering","level":1,"score":0.3221718668937683},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.29839491844177246},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21471071243286133},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.18103983998298645},{"id":"https://openalex.org/C2775924081","wikidata":"https://www.wikidata.org/wiki/Q55608371","display_name":"Control (management)","level":2,"score":0.15435945987701416},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005246","descriptor_name":"Feedback","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005246","descriptor_name":"Feedback","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005246","descriptor_name":"Feedback","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060846","descriptor_name":"Cell Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D060846","descriptor_name":"Cell Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D060846","descriptor_name":"Cell Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1109/embc.2016.7590984","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2016.7590984","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},{"id":"pmid:28268601","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28268601","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W2015628012","https://openalex.org/W2016876355","https://openalex.org/W2042990867","https://openalex.org/W2053618966","https://openalex.org/W2067833766","https://openalex.org/W2077206871","https://openalex.org/W2109295942","https://openalex.org/W2117820574","https://openalex.org/W2144981148","https://openalex.org/W2152585118","https://openalex.org/W2155306598","https://openalex.org/W2331384579","https://openalex.org/W2479263976","https://openalex.org/W2560320833","https://openalex.org/W3187945838"],"related_works":["https://openalex.org/W4391375266","https://openalex.org/W2748952813","https://openalex.org/W2390279801","https://openalex.org/W2358668433","https://openalex.org/W4396701345","https://openalex.org/W2376932109","https://openalex.org/W2001405890","https://openalex.org/W4396696052","https://openalex.org/W4402327032","https://openalex.org/W2382290278"],"abstract_inverted_index":{"Cellular":[0],"engineering":[1,32],"involves":[2,33],"modification":[3],"and":[4,9,15,36,65,77,80,143,148,155,164,184,192],"control":[5],"of":[6,13,17,25,38,49,61,124,194],"cell":[7,42,68,85],"properties,":[8],"requires":[10],"an":[11,113,128],"understanding":[12,159],"fundamentals":[14],"mechanisms":[16],"action":[18],"for":[19,116,131,175],"cellular":[20,31],"derived":[21],"product":[22],"development.":[23],"One":[24],"the":[26,34,50,59,84,98,117,125],"keys":[27],"to":[28,171,189],"success":[29],"in":[30,83,93,169],"quality":[35],"validity":[37],"results":[39],"obtained":[40],"from":[41],"chemical":[43,69,86],"signaling":[44,70,87],"pathway":[45,71,88,99,140,153,162],"assays.":[46],"The":[47,158],"accuracy":[48,191],"assay":[51,94,108,132,176,185],"data":[52,95],"cannot":[53],"be":[54],"verified":[55],"or":[56],"assured":[57],"if":[58],"effect":[60],"positive":[62,81],"feedback,":[63],"nonlinearities,":[64],"interrelationships":[66],"between":[67,182],"elements":[72],"are":[73],"not":[74],"understood,":[75],"modeled,":[76],"simulated.":[78],"Nonlinearities":[79],"feedback":[82,146],"can":[89,100],"produce":[90],"significant":[91],"aberrations":[92],"collection.":[96],"Simulating":[97],"reveal":[101],"potential":[102],"instability":[103],"problems":[104],"that":[105],"will":[106],"affect":[107],"results.":[109,195],"A":[110],"simulation,":[111],"using":[112],"electrical":[114],"analog":[115],"coupled":[118],"differential":[119],"equations":[120],"representing":[121],"each":[122],"segment":[123],"pathway,":[126],"provides":[127],"excellent":[129],"tool":[130],"validation":[133],"purposes.":[134],"With":[135],"this":[136],"approach,":[137],"voltages":[138],"represent":[139],"enzyme":[141],"concentrations":[142],"operational":[144],"amplifier":[145],"resistance":[147,150],"input":[149],"values":[151],"determine":[152],"gain":[154],"rate":[156],"constants.":[157],"provided":[160],"by":[161],"modeling":[163],"simulation":[165],"is":[166],"strategically":[167],"important":[168],"order":[170],"establish":[172],"experimental":[173],"controls":[174],"protocol":[177],"structure,":[178],"time":[179],"frames":[180],"specified":[181],"assays,":[183],"concentration":[186],"variation":[187],"limits;":[188],"ensure":[190],"reproducibility":[193]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}