{"id":"https://openalex.org/W2533243618","doi":"https://doi.org/10.1109/embc.2016.7590922","title":"Automated tracing of microglia using multilevel thresholding and minimum spanning trees","display_name":"Automated tracing of microglia using multilevel thresholding and minimum spanning trees","publication_year":2016,"publication_date":"2016-08-01","ids":{"openalex":"https://openalex.org/W2533243618","doi":"https://doi.org/10.1109/embc.2016.7590922","mag":"2533243618","pmid":"https://pubmed.ncbi.nlm.nih.gov/28268542"},"language":"en","primary_location":{"id":"doi:10.1109/embc.2016.7590922","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2016.7590922","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023565964","display_name":"Mahmoud Abdolhoseini","orcid":"https://orcid.org/0000-0002-7047-2084"},"institutions":[{"id":"https://openalex.org/I78757542","display_name":"University of Newcastle Australia","ror":"https://ror.org/00eae9z71","country_code":"AU","type":"education","lineage":["https://openalex.org/I78757542"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Mahmoud Abdolhoseini","raw_affiliation_strings":["School of Electrical Eng and Computer Science, The University of Newcastle, NSW"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Electrical Eng and Computer Science, The University of Newcastle, NSW","institution_ids":["https://openalex.org/I78757542"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051710706","display_name":"Frederick R. Walker","orcid":"https://orcid.org/0000-0002-9068-0761"},"institutions":[{"id":"https://openalex.org/I78757542","display_name":"University of Newcastle Australia","ror":"https://ror.org/00eae9z71","country_code":"AU","type":"education","lineage":["https://openalex.org/I78757542"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Frederick Walker","raw_affiliation_strings":["School of Biomedical Sciences and Pharmacy, The University of Newcastle"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biomedical Sciences and Pharmacy, The University of Newcastle","institution_ids":["https://openalex.org/I78757542"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5010753349","display_name":"Sarah J. Johnson","orcid":"https://orcid.org/0000-0001-6011-3013"},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sarah Johnson","raw_affiliation_strings":["School of Electrical Eng and Computer Science, The University of Newcastle"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Electrical Eng and Computer Science, The University of Newcastle","institution_ids":["https://openalex.org/I84884186"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5023565964"],"corresponding_institution_ids":["https://openalex.org/I78757542"],"apc_list":null,"apc_paid":null,"fwci":1.4171,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.83117856,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":98},"biblio":{"volume":"2016","issue":null,"first_page":"1208","last_page":"1211"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.9994000196456909,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.9994000196456909,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.995199978351593,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.9929999709129333,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thresholding","display_name":"Thresholding","score":0.808959424495697},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6598243713378906},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.6399087905883789},{"id":"https://openalex.org/keywords/tracing","display_name":"Tracing","score":0.6035316586494446},{"id":"https://openalex.org/keywords/pixel","display_name":"Pixel","score":0.5553311109542847},{"id":"https://openalex.org/keywords/minimum-spanning-tree","display_name":"Minimum spanning tree","score":0.5170236825942993},{"id":"https://openalex.org/keywords/a-priori-and-a-posteriori","display_name":"A priori and a posteriori","score":0.5051527619361877},{"id":"https://openalex.org/keywords/pattern-recognition","display_name":"Pattern recognition (psychology)","score":0.49688437581062317},{"id":"https://openalex.org/keywords/tree","display_name":"Tree (set theory)","score":0.4596303403377533},{"id":"https://openalex.org/keywords/spanning-tree","display_name":"Spanning tree","score":0.41155749559402466},{"id":"https://openalex.org/keywords/microglia","display_name":"Microglia","score":0.41139766573905945},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.39986199140548706},{"id":"https://openalex.org/keywords/image","display_name":"Image (mathematics)","score":0.2908620834350586},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.21727994084358215},{"id":"https://openalex.org/keywords/algorithm","display_name":"Algorithm","score":0.16629967093467712},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.07347691059112549}],"concepts":[{"id":"https://openalex.org/C191178318","wikidata":"https://www.wikidata.org/wiki/Q2256906","display_name":"Thresholding","level":3,"score":0.808959424495697},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6598243713378906},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.6399087905883789},{"id":"https://openalex.org/C138673069","wikidata":"https://www.wikidata.org/wiki/Q322229","display_name":"Tracing","level":2,"score":0.6035316586494446},{"id":"https://openalex.org/C160633673","wikidata":"https://www.wikidata.org/wiki/Q355198","display_name":"Pixel","level":2,"score":0.5553311109542847},{"id":"https://openalex.org/C13743678","wikidata":"https://www.wikidata.org/wiki/Q240464","display_name":"Minimum spanning tree","level":2,"score":0.5170236825942993},{"id":"https://openalex.org/C75553542","wikidata":"https://www.wikidata.org/wiki/Q178161","display_name":"A priori and a posteriori","level":2,"score":0.5051527619361877},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.49688437581062317},{"id":"https://openalex.org/C113174947","wikidata":"https://www.wikidata.org/wiki/Q2859736","display_name":"Tree (set theory)","level":2,"score":0.4596303403377533},{"id":"https://openalex.org/C64331007","wikidata":"https://www.wikidata.org/wiki/Q831672","display_name":"Spanning tree","level":2,"score":0.41155749559402466},{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.41139766573905945},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.39986199140548706},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.2908620834350586},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.21727994084358215},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.16629967093467712},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.07347691059112549},{"id":"https://openalex.org/C111472728","wikidata":"https://www.wikidata.org/wiki/Q9471","display_name":"Epistemology","level":1,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.0},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007090","descriptor_name":"Image Interpretation, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008853","descriptor_name":"Microscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008853","descriptor_name":"Microscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008853","descriptor_name":"Microscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017628","descriptor_name":"Microglia","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D017628","descriptor_name":"Microglia","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D017628","descriptor_name":"Microglia","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1109/embc.2016.7590922","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2016.7590922","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},{"id":"pmid:28268542","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28268542","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference","raw_type":null},{"id":"pmh:uon:25386","is_oa":false,"landing_page_url":"http://hdl.handle.net/1959.13/1326241","pdf_url":null,"source":{"id":"https://openalex.org/S4377196471","display_name":"NOVA (University of Newcastle Australia)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I78757542","host_organization_name":"University of Newcastle Australia","host_organization_lineage":["https://openalex.org/I78757542"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"conference paper"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/15","score":0.44999998807907104,"display_name":"Life in Land"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W173577600","https://openalex.org/W1964041350","https://openalex.org/W1967450909","https://openalex.org/W1999244633","https://openalex.org/W2022037436","https://openalex.org/W2044219916","https://openalex.org/W2061628168","https://openalex.org/W2066943555","https://openalex.org/W2067499927","https://openalex.org/W2084716923","https://openalex.org/W2113682088","https://openalex.org/W2119860717","https://openalex.org/W2133059825","https://openalex.org/W2142332605","https://openalex.org/W2142659995","https://openalex.org/W2158536428","https://openalex.org/W2163398148","https://openalex.org/W2228956989","https://openalex.org/W2285721383","https://openalex.org/W2489206674","https://openalex.org/W4246422566","https://openalex.org/W4300314589"],"related_works":["https://openalex.org/W2099915933","https://openalex.org/W2418231958","https://openalex.org/W4380840098","https://openalex.org/W3116169590","https://openalex.org/W1703428550","https://openalex.org/W2044443696","https://openalex.org/W3101281637","https://openalex.org/W2390742649","https://openalex.org/W1561849181","https://openalex.org/W3081212354"],"abstract_inverted_index":{"Microglia":[0],"are":[1],"immune":[2],"cells":[3],"of":[4,20,56,94,126],"the":[5,103,106],"central":[6],"nervous":[7],"system.":[8],"Good":[9],"knowledge":[10],"about":[11],"their":[12,21],"morphology":[13],"leads":[14],"us":[15],"to":[16,31,59,70,78,105,110],"a":[17,79,83,98],"better":[18],"understanding":[19],"functionality.":[22],"In":[23],"this":[24,112],"article":[25],"we":[26,63,101],"propose":[27],"an":[28],"automated":[29],"method":[30,119],"trace":[32],"microglia":[33,95],"in":[34,124],"microscopy":[35],"images.":[36,128],"Our":[37],"approach":[38],"is":[39,96,120],"powered":[40],"by":[41],"two":[42],"main":[43],"algorithms:":[44],"multilevel":[45],"thresholding":[46],"(MT)":[47],"and":[48,81,122],"minimum":[49],"spanning":[50],"tree":[51,92],"(MST).":[52],"MT":[53],"quantizes":[54],"intensities":[55],"image":[57],"pixels":[58],"several":[60],"levels,":[61],"then":[62],"sample":[64],"each":[65],"level":[66,80],"with":[67],"different":[68],"rates":[69],"produce":[71],"seed":[72,75,108],"points.":[73],"Each":[74],"point":[76],"belongs":[77],"has":[82],"specific":[84],"value,":[85],"therefore":[86],"they":[87],"can":[88],"be":[89],"prioritized.":[90],"The":[91],"structure":[93],"known":[97],"priori,":[99],"so":[100],"apply":[102],"MST":[104],"prioritized":[107],"points":[109],"preserve":[111],"feature.":[113],"Results":[114],"show":[115],"that":[116],"our":[117],"proposed":[118],"fast":[121],"accurate":[123],"reconstruction":[125],"large":[127]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4}],"updated_date":"2026-04-29T09:16:38.111599","created_date":"2025-10-10T00:00:00"}