{"id":"https://openalex.org/W2294637582","doi":"https://doi.org/10.1109/embc.2015.7318521","title":"Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology","display_name":"Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology","publication_year":2015,"publication_date":"2015-08-01","ids":{"openalex":"https://openalex.org/W2294637582","doi":"https://doi.org/10.1109/embc.2015.7318521","mag":"2294637582","pmid":"https://pubmed.ncbi.nlm.nih.gov/26736421"},"language":"en","primary_location":{"id":"doi:10.1109/embc.2015.7318521","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2015.7318521","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},"type":"conference-paper","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080904375","display_name":"Lorenzo Pasotti","orcid":"https://orcid.org/0000-0002-5431-3083"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Lorenzo Pasotti","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062705104","display_name":"Susanna Zucca","orcid":"https://orcid.org/0000-0001-8465-632X"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Susanna Zucca","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006570922","display_name":"Michela Casanova","orcid":"https://orcid.org/0000-0003-0368-7883"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Michela Casanova","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062152131","display_name":"Nicol\u00f2 Politi","orcid":null},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Nicolo' Politi","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027397773","display_name":"Ilaria Massaiu","orcid":"https://orcid.org/0000-0001-5805-1909"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Ilaria Massaiu","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111735796","display_name":"Giuliano Mazzini","orcid":null},"institutions":[{"id":"https://openalex.org/I4210155236","display_name":"National Research Council","ror":"https://ror.org/04zaypm56","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Giuliano Mazzini","raw_affiliation_strings":["The Institute of Molecular Genetics, National Resource Council, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Institute of Molecular Genetics, National Resource Council, Pavia, Italy","institution_ids":["https://openalex.org/I4210155236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064428733","display_name":"G. Micoli","orcid":"https://orcid.org/0000-0003-3168-6976"},"institutions":[{"id":"https://openalex.org/I4210105320","display_name":"Fondazione Salvatore Maugeri","ror":"https://ror.org/01e8tvg28","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I4210105320","https://openalex.org/I4210153126"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Giuseppina Micoli","raw_affiliation_strings":["The Centro di Ricerche Ambientali, IRCCS Fondazione Salvatore Maugeri, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Centro di Ricerche Ambientali, IRCCS Fondazione Salvatore Maugeri, Pavia, Italy","institution_ids":["https://openalex.org/I4210105320"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078908847","display_name":"Cinzia Calvio","orcid":"https://orcid.org/0000-0003-1631-0452"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Cinzia Calvio","raw_affiliation_strings":["The Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d, University of Pavia, Pavia, Italy","The Department of Biology and Biotechnology \"Lazzaro Spallanzani\", University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]},{"raw_affiliation_string":"The Department of Biology and Biotechnology \"Lazzaro Spallanzani\", University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063579573","display_name":"Maria Gabriella Cusella De Angelis","orcid":"https://orcid.org/0000-0003-2642-3346"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Maria Gabriella Cusella De Angelis","raw_affiliation_strings":["The Department of Public Health, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Department of Public Health, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081448028","display_name":"Paolo Magni","orcid":"https://orcid.org/0000-0002-8931-4676"},"institutions":[{"id":"https://openalex.org/I25217355","display_name":"University of Pavia","ror":"https://ror.org/00s6t1f81","country_code":"IT","type":"education","lineage":["https://openalex.org/I25217355"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Paolo Magni","raw_affiliation_strings":["Department of Electrical, University of Pavia, Pavia, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Electrical, University of Pavia, Pavia, Italy","institution_ids":["https://openalex.org/I25217355"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1769,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.82755946,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"2015","issue":null,"first_page":"953","last_page":"956"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9878000020980835,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactose","display_name":"Lactose","score":0.701513409614563},{"id":"https://openalex.org/keywords/biofuel","display_name":"Biofuel","score":0.6630212664604187},{"id":"https://openalex.org/keywords/food-waste","display_name":"Food waste","score":0.6204513907432556},{"id":"https://openalex.org/keywords/biochemical-engineering","display_name":"Biochemical engineering","score":0.57085120677948},{"id":"https://openalex.org/keywords/waste-management","display_name":"Waste management","score":0.5263527631759644},{"id":"https://openalex.org/keywords/fermentation","display_name":"Fermentation","score":0.5169093608856201},{"id":"https://openalex.org/keywords/environmental-science","display_name":"Environmental science","score":0.4626188576221466},{"id":"https://openalex.org/keywords/biotechnology","display_name":"Biotechnology","score":0.4558599591255188},{"id":"https://openalex.org/keywords/ethanol-fuel","display_name":"Ethanol fuel","score":0.4551042318344116},{"id":"https://openalex.org/keywords/bioconversion","display_name":"Bioconversion","score":0.4527592062950134},{"id":"https://openalex.org/keywords/production","display_name":"Production (economics)","score":0.4476739168167114},{"id":"https://openalex.org/keywords/food-science","display_name":"Food science","score":0.3067280054092407},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.26602065563201904},{"id":"https://openalex.org/keywords/engineering","display_name":"Engineering","score":0.2102709710597992},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.17527586221694946}],"concepts":[{"id":"https://openalex.org/C2778724459","wikidata":"https://www.wikidata.org/wiki/Q127900","display_name":"Lactose","level":2,"score":0.701513409614563},{"id":"https://openalex.org/C53991642","wikidata":"https://www.wikidata.org/wiki/Q128991","display_name":"Biofuel","level":2,"score":0.6630212664604187},{"id":"https://openalex.org/C2777499484","wikidata":"https://www.wikidata.org/wiki/Q18919","display_name":"Food waste","level":2,"score":0.6204513907432556},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.57085120677948},{"id":"https://openalex.org/C548081761","wikidata":"https://www.wikidata.org/wiki/Q180388","display_name":"Waste management","level":1,"score":0.5263527631759644},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.5169093608856201},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.4626188576221466},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.4558599591255188},{"id":"https://openalex.org/C65183824","wikidata":"https://www.wikidata.org/wiki/Q194361","display_name":"Ethanol fuel","level":3,"score":0.4551042318344116},{"id":"https://openalex.org/C2780074830","wikidata":"https://www.wikidata.org/wiki/Q864423","display_name":"Bioconversion","level":3,"score":0.4527592062950134},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.4476739168167114},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3067280054092407},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.26602065563201904},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.2102709710597992},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17527586221694946},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000431","descriptor_name":"Ethanol","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002611","descriptor_name":"Cheese","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002611","descriptor_name":"Cheese","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002611","descriptor_name":"Cheese","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007785","descriptor_name":"Lactose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007785","descriptor_name":"Lactose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007785","descriptor_name":"Lactose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056804","descriptor_name":"Biofuels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1109/embc.2015.7318521","is_oa":false,"landing_page_url":"https://doi.org/10.1109/embc.2015.7318521","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)","raw_type":"proceedings-article"},{"id":"pmid:26736421","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26736421","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/12","score":0.4000000059604645,"display_name":"Responsible consumption and production"}],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1531337437","https://openalex.org/W1559760291","https://openalex.org/W1972215573","https://openalex.org/W1973577260","https://openalex.org/W1982228455","https://openalex.org/W2034293258","https://openalex.org/W2043151065","https://openalex.org/W2053181423","https://openalex.org/W2058498605","https://openalex.org/W2065063088","https://openalex.org/W2065889411","https://openalex.org/W2068773609","https://openalex.org/W2069499427","https://openalex.org/W2088631531","https://openalex.org/W2095998067","https://openalex.org/W2110821951","https://openalex.org/W2130245744","https://openalex.org/W2164028644","https://openalex.org/W2164563592","https://openalex.org/W2170449887","https://openalex.org/W2770893645","https://openalex.org/W6746334690"],"related_works":["https://openalex.org/W2014259751","https://openalex.org/W2366528792","https://openalex.org/W2350960133","https://openalex.org/W2388053644","https://openalex.org/W2123253453","https://openalex.org/W2340976201","https://openalex.org/W4296625129","https://openalex.org/W2591045632","https://openalex.org/W3215486597","https://openalex.org/W1518817075"],"abstract_inverted_index":{"Whey":[0],"is":[1,11,48],"an":[2,96],"abundant":[3],"by-product":[4],"of":[5,64,76],"cheese":[6],"production":[7],"process":[8],"and":[9,22,80,94,111],"it":[10],"considered":[12,50],"a":[13,52,77],"special":[14],"waste":[15,35,54,79],"due":[16,55],"to":[17,56,92],"its":[18,57],"high":[19,58],"nutritional":[20],"load":[21],"hypertrophic":[23],"potential.":[24],"Technologies":[25],"for":[26],"whey":[27,40],"valorization":[28],"are":[29],"available.":[30],"They":[31],"can":[32,100],"convert":[33],"such":[34],"into":[36,66],"high-value":[37],"products,":[38],"like":[39],"proteins.":[41],"However,":[42],"the":[43,88,103],"remaining":[44],"liquid":[45],"(called":[46],"permeate)":[47],"still":[49],"as":[51],"polluting":[53],"lactose":[59,65],"concentration.":[60],"The":[61],"alcoholic":[62],"fermentation":[63],"ethanol":[67],"will":[68],"simultaneously":[69],"achieve":[70],"two":[71],"important":[72],"goals:":[73],"safe":[74],"disposal":[75],"pollutant":[78],"green":[81],"energy":[82],"production.":[83],"This":[84],"methodology":[85],"paper":[86],"illustrates":[87],"workflow":[89],"carried":[90],"out":[91],"design":[93],"realize":[95],"optimized":[97],"microorganism":[98],"that":[99],"efficiently":[101],"perform":[102],"lactose-to-ethanol":[104],"conversion,":[105],"engineered":[106],"via":[107],"synthetic":[108],"biology":[109],"experimental":[110],"computational":[112],"approaches.":[113]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1}],"updated_date":"2026-07-29T14:22:42.915294","created_date":"2016-06-24T00:00:00"}