{"id":"https://openalex.org/W1996962371","doi":"https://doi.org/10.1109/bmei.2011.6098681","title":"Development of transgenic Phalaenopsis resistant to two viral infections","display_name":"Development of transgenic Phalaenopsis resistant to two viral infections","publication_year":2011,"publication_date":"2011-10-01","ids":{"openalex":"https://openalex.org/W1996962371","doi":"https://doi.org/10.1109/bmei.2011.6098681","mag":"1996962371"},"language":"en","primary_location":{"id":"doi:10.1109/bmei.2011.6098681","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2011.6098681","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 4th International Conference on Biomedical Engineering and Informatics (BMEI)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102148063","display_name":"Shuguo Fan","orcid":null},"institutions":[{"id":"https://openalex.org/I4210143356","display_name":"Chuxiong Normal University","ror":"https://ror.org/04c14yn55","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210143356"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shuguo Fan","raw_affiliation_strings":["Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, China","Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong 675000, China"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, China","institution_ids":["https://openalex.org/I4210143356"]},{"raw_affiliation_string":"Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong 675000, China","institution_ids":["https://openalex.org/I4210143356"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5102148063"],"corresponding_institution_ids":["https://openalex.org/I4210143356"],"apc_list":null,"apc_paid":null,"fwci":0.1303,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.5088382,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"37","issue":null,"first_page":"1963","last_page":"1966"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phalaenopsis","display_name":"Phalaenopsis","score":0.8772464394569397},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.6625477075576782},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.576799213886261},{"id":"https://openalex.org/keywords/virology","display_name":"Virology","score":0.5754109621047974},{"id":"https://openalex.org/keywords/transgene","display_name":"Transgene","score":0.5263934135437012},{"id":"https://openalex.org/keywords/southern-blot","display_name":"Southern blot","score":0.494733065366745},{"id":"https://openalex.org/keywords/selection","display_name":"Selection (genetic algorithm)","score":0.48818689584732056},{"id":"https://openalex.org/keywords/genetically-modified-crops","display_name":"Genetically modified crops","score":0.46186286211013794},{"id":"https://openalex.org/keywords/molecular-biology","display_name":"Molecular biology","score":0.4028772711753845},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.3143109083175659},{"id":"https://openalex.org/keywords/botany","display_name":"Botany","score":0.22877731919288635}],"concepts":[{"id":"https://openalex.org/C2779207651","wikidata":"https://www.wikidata.org/wiki/Q133897","display_name":"Phalaenopsis","level":2,"score":0.8772464394569397},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6625477075576782},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.576799213886261},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5754109621047974},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.5263934135437012},{"id":"https://openalex.org/C133307689","wikidata":"https://www.wikidata.org/wiki/Q48749","display_name":"Southern blot","level":3,"score":0.494733065366745},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.48818689584732056},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.46186286211013794},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4028772711753845},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3143109083175659},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.22877731919288635},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/bmei.2011.6098681","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2011.6098681","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 4th International Conference on Biomedical Engineering and Informatics (BMEI)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Life in Land","score":0.6000000238418579,"id":"https://metadata.un.org/sdg/15"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W1963519683","https://openalex.org/W1965944947","https://openalex.org/W1977091294","https://openalex.org/W1992075432","https://openalex.org/W1994919921","https://openalex.org/W2032463086","https://openalex.org/W2061506729","https://openalex.org/W2158493418","https://openalex.org/W2379734693","https://openalex.org/W7061430888"],"related_works":["https://openalex.org/W4385962901","https://openalex.org/W2693754724","https://openalex.org/W2198747735","https://openalex.org/W307256944","https://openalex.org/W2121130226","https://openalex.org/W3014497569","https://openalex.org/W4379141261","https://openalex.org/W1597171259","https://openalex.org/W2266971042","https://openalex.org/W2793991214"],"abstract_inverted_index":{"Cymbididum":[0],"mosaic":[1],"virus":[2,7],"(CymMV)":[3],"and":[4,23,37,51,67,98,107,117,131,140,145,155,179],"Odontoglossum":[5],"ringspot":[6],"(ORSV)":[8],"are":[9],"the":[10,13,29,35,43,46,52,123,136,163,171,181,188],"two":[11,189],"of":[12,34,49,122,138,143],"most":[14],"prevalent":[15],"plant":[16],"viruses":[17],"in":[18,95,112],"orchids.":[19],"To":[20],"generate":[21],"CymMV":[22,36,50,144],"ORSV-resistant":[24],"Phalaenopsis":[25],"amabilis":[26,151,174],"overexpressing":[27],"either":[28],"sense":[30,139],"or":[31],"anti-sense":[32,141],"strand":[33,142],"ORSV":[38,53,146],"coat":[39],"protein":[40],"(CP)":[41],"genes,":[42],"gene":[44],"comprising":[45],"capsid":[47],"proteins":[48],"has":[54,175],"been":[55,63,71,160,176],"constructed,":[56],"optimum":[57],"conditions":[58],"for":[59,81,129,162],"particle":[60],"bombardment":[61],"have":[62,70,158],"investigated.":[64],"Resistant":[65],"calli":[66],"transgenic":[68,172,182],"plantlets":[69],"screened":[72],"on":[73],"selection":[74,77,90,111],"medium.":[75,100],"The":[76],"medium":[78,114],"was":[79,93,102,115],"optimized":[80],"shoot":[82],"induction":[83],"with":[84],"L-methionine":[85],"sulfoximine":[86],"(MSO)":[87],"added":[88],"as":[89],"agent.":[91],"Selection":[92],"done":[94,161],"both":[96],"solid":[97],"liquid":[99,105,113],"It":[101],"found":[103],"that":[104,110,170],"media":[106],"experiment":[108],"showed":[109,169],"faster":[116],"more":[118],"efficient.":[119],"PCR":[120,132],"amplification":[121],"inserted":[124],"CP":[125,147],"genes":[126,148],"were":[127],"used":[128],"genotyping":[130],"Southern":[133],"assay":[134],"confirm":[135],"insertion":[137],"into":[149],"P.":[150,173],"transformants.":[152],"Northern":[153],"blot":[154,157],"Western":[156],"also":[159],"putative":[164],"transformed":[165],"plants.":[166],"Our":[167],"results":[168],"successfully":[177],"created,":[178],"hopefully,":[180],"orchids":[183],"will":[184],"be":[185],"resistant":[186],"against":[187],"viral":[190],"infection.":[191]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}