{"id":"https://openalex.org/W2010448983","doi":"https://doi.org/10.1109/bmei.2011.6098577","title":"Cloning, expression and characterization of a thermostable pullulanase from newly isolated thermophilic Geobacillus sp. LM14–3","display_name":"Cloning, expression and characterization of a thermostable pullulanase from newly isolated thermophilic Geobacillus sp. LM14–3","publication_year":2011,"publication_date":"2011-10-01","ids":{"openalex":"https://openalex.org/W2010448983","doi":"https://doi.org/10.1109/bmei.2011.6098577","mag":"2010448983"},"language":"en","primary_location":{"id":"doi:10.1109/bmei.2011.6098577","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2011.6098577","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 4th International Conference on Biomedical Engineering and Informatics (BMEI)","raw_type":"proceedings-article"},"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102612443","display_name":"Shaojing Sun","orcid":null},"institutions":[{"id":"https://openalex.org/I132369690","display_name":"Tianjin University of Science and Technology","ror":"https://ror.org/018rbtf37","country_code":"CN","type":"education","lineage":["https://openalex.org/I132369690"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shaojing Sun","raw_affiliation_strings":["Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China","institution_ids":["https://openalex.org/I132369690"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060741317","display_name":"Fuping Lu","orcid":"https://orcid.org/0000-0002-0610-8827"},"institutions":[{"id":"https://openalex.org/I132369690","display_name":"Tianjin University of Science and Technology","ror":"https://ror.org/018rbtf37","country_code":"CN","type":"education","lineage":["https://openalex.org/I132369690"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fuping Lu","raw_affiliation_strings":["Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China","institution_ids":["https://openalex.org/I132369690"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007162529","display_name":"Hui Song","orcid":"https://orcid.org/0000-0003-2642-1850"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hui Song","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085975071","display_name":"Jianyong Xu","orcid":"https://orcid.org/0000-0003-1910-4603"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianyong Xu","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066087154","display_name":"Nan Jiang","orcid":"https://orcid.org/0000-0003-4211-1385"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Nan Jiang","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101993717","display_name":"Yueling Zhang","orcid":"https://orcid.org/0000-0002-5619-8410"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yueling Zhang","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100361348","display_name":"Li Li","orcid":"https://orcid.org/0000-0003-2342-3658"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Li","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Tan Ming","orcid":null},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tan Ming","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5024296613","display_name":"Wen Zheng","orcid":"https://orcid.org/0000-0002-3686-5765"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen Zheng","raw_affiliation_strings":["Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China"],"affiliations":[{"raw_affiliation_string":"Application of Genetic Engineering and Microbial Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences (CAS), Tianjin, China","institution_ids":["https://openalex.org/I4210139473","https://openalex.org/I19820366"]},{"raw_affiliation_string":"Application of Genetic Engineering and Microbial, Technology Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese, Academy of Sciences, Tianjin, China","institution_ids":["https://openalex.org/I19820366"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":9,"corresponding_author_ids":["https://openalex.org/A5102612443"],"corresponding_institution_ids":["https://openalex.org/I132369690"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.13861301,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"26","issue":null,"first_page":"1567","last_page":"1570"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12991","display_name":"Phytase and its Applications","score":0.9861000180244446,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pullulanase","display_name":"Pullulanase","score":0.9875591993331909},{"id":"https://openalex.org/keywords/pullulan","display_name":"Pullulan","score":0.7754695415496826},{"id":"https://openalex.org/keywords/maltotriose","display_name":"Maltotriose","score":0.750022292137146},{"id":"https://openalex.org/keywords/thermophile","display_name":"Thermophile","score":0.7417182326316833},{"id":"https://openalex.org/keywords/geobacillus-stearothermophilus","display_name":"Geobacillus stearothermophilus","score":0.7045876979827881},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.49373993277549744},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.4579100012779236},{"id":"https://openalex.org/keywords/hydrolysis","display_name":"Hydrolysis","score":0.43839728832244873},{"id":"https://openalex.org/keywords/fermentation","display_name":"Fermentation","score":0.42922377586364746},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.35579437017440796},{"id":"https://openalex.org/keywords/enzyme","display_name":"Enzyme","score":0.3450111150741577},{"id":"https://openalex.org/keywords/polysaccharide","display_name":"Polysaccharide","score":0.17672932147979736},{"id":"https://openalex.org/keywords/maltose","display_name":"Maltose","score":0.0912749171257019}],"concepts":[{"id":"https://openalex.org/C2779423947","wikidata":"https://www.wikidata.org/wiki/Q909242","display_name":"Pullulanase","level":3,"score":0.9875591993331909},{"id":"https://openalex.org/C2779257994","wikidata":"https://www.wikidata.org/wiki/Q419779","display_name":"Pullulan","level":3,"score":0.7754695415496826},{"id":"https://openalex.org/C2780095022","wikidata":"https://www.wikidata.org/wiki/Q249208","display_name":"Maltotriose","level":4,"score":0.750022292137146},{"id":"https://openalex.org/C27911776","wikidata":"https://www.wikidata.org/wiki/Q834023","display_name":"Thermophile","level":3,"score":0.7417182326316833},{"id":"https://openalex.org/C2909197731","wikidata":"https://www.wikidata.org/wiki/Q136876","display_name":"Geobacillus stearothermophilus","level":4,"score":0.7045876979827881},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.49373993277549744},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4579100012779236},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.43839728832244873},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.42922377586364746},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.35579437017440796},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3450111150741577},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.17672932147979736},{"id":"https://openalex.org/C2778197599","wikidata":"https://www.wikidata.org/wiki/Q170002","display_name":"Maltose","level":3,"score":0.0912749171257019}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1109/bmei.2011.6098577","is_oa":false,"landing_page_url":"https://doi.org/10.1109/bmei.2011.6098577","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2011 4th International Conference on Biomedical Engineering and Informatics (BMEI)","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W2073472591","https://openalex.org/W2083162875","https://openalex.org/W2095789984","https://openalex.org/W2097382368","https://openalex.org/W2105734162","https://openalex.org/W2110899053","https://openalex.org/W2112207535","https://openalex.org/W2120024227","https://openalex.org/W2144094477","https://openalex.org/W2155776392","https://openalex.org/W2159973324","https://openalex.org/W2169243699","https://openalex.org/W2291209920","https://openalex.org/W2409430954","https://openalex.org/W2463167738","https://openalex.org/W2472297647","https://openalex.org/W4253577720","https://openalex.org/W6719315001"],"related_works":["https://openalex.org/W2074033167","https://openalex.org/W1984800721","https://openalex.org/W2001613509","https://openalex.org/W2282813785","https://openalex.org/W2020521526","https://openalex.org/W2087760382","https://openalex.org/W1989215918","https://openalex.org/W1950908415","https://openalex.org/W2076752454","https://openalex.org/W2080978915"],"abstract_inverted_index":{"A":[0],"novel":[1],"strain":[2,27],"LM14-3":[3],"producing":[4],"thermostable":[5,35,136],"pullulanase":[6,36,57,71,77,109,137],"was":[7,28,45,62,72,84,120],"separated":[8],"from":[9],"Lunma":[10],"hot":[11],"spring":[12],"of":[13,16,51,69,76,95,126],"Yunnan":[14],"Province":[15],"China.":[17],"According":[18],"to":[19,80,122],"colony":[20],"morphology":[21],"and":[22,41,151],"16S":[23],"rRNA":[24],"sequence,":[25],"this":[26,140],"named":[29],"as":[30,60,130,154,156],"Geobacillus":[31,52],"sp.":[32,53],"LM14-3.":[33,54],"The":[34,55,66,108,135],"with":[37,74],"optimal":[38,42],"pH":[39],"6.0":[40],"temperature":[43,149],"60\u00b0C":[44],"accumulated":[46],"in":[47,86,139],"the":[48,70,91,124,163],"fermentation":[49],"broth":[50],"corresponding":[56],"gene,":[58],"designated":[59],"pul-3,":[61],"obtained":[63],"through":[64],"PCR.":[65],"domain":[67],"structure":[68],"consistent":[73],"that":[75],"I":[78],"according":[79],"sequence":[81],"similarity.":[82],"Pul-3":[83],"expressed":[85],"E.":[87],"coli.":[88],"Maltotriose":[89],"is":[90],"unique":[92],"hydrolysis":[93],"product":[94],"pullulan":[96],"determined":[97],"by":[98],"HPLC,":[99],"which":[100,119,161],"confirmed":[101],"its":[102],"high":[103,117],"specificity":[104],"for":[105],"alpha-1,6-glycosidic":[106],"bond.":[107],"reported":[110],"previously":[111],"usually":[112],"lost":[113],"their":[114],"activity":[115],"under":[116],"temperature,":[118],"difficult":[121],"satisfy":[123],"demand":[125],"biotechnological":[127],"applications,":[128],"such":[129],"sugar":[131],"making,":[132],"detergent,":[133],"etc.":[134],"found":[138],"study":[141],"owned":[142],"excellent":[143],"thermal":[144],"stability":[145],"over":[146],"a":[147,152],"wide":[148],"span,":[150],"half-life":[153],"long":[155],"56":[157],"h":[158],"at":[159],"60\u00b0C,":[160],"favors":[162],"potential":[164],"industrial":[165],"application.":[166]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-04-16T08:26:57.006410","created_date":"2025-10-10T00:00:00"}