{"id":"https://openalex.org/W3027246467","doi":"https://doi.org/10.1109/access.2020.2996201","title":"A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection","display_name":"A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection","publication_year":2020,"publication_date":"2020-01-01","ids":{"openalex":"https://openalex.org/W3027246467","doi":"https://doi.org/10.1109/access.2020.2996201","mag":"3027246467"},"language":"en","primary_location":{"id":"doi:10.1109/access.2020.2996201","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2020.2996201","pdf_url":"https://ieeexplore.ieee.org/ielx7/6287639/8948470/09097833.pdf","source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://ieeexplore.ieee.org/ielx7/6287639/8948470/09097833.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101817221","display_name":"Di Wu","orcid":"https://orcid.org/0000-0002-0245-8205"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210088164","display_name":"Changchun Institute of Optics, Fine Mechanics and Physics","ror":"https://ror.org/012rct222","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Di Wu","raw_affiliation_strings":["Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China","University of Chinese Academy of Sciences (UCAS), Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]},{"raw_affiliation_string":"University of Chinese Academy of Sciences (UCAS), Beijing, China","institution_ids":["https://openalex.org/I4210165038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029130019","display_name":"Bing Shi","orcid":"https://orcid.org/0000-0001-9117-1945"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210088164","display_name":"Changchun Institute of Optics, Fine Mechanics and Physics","ror":"https://ror.org/012rct222","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bing Shi","raw_affiliation_strings":["Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100344023","display_name":"Bin Li","orcid":"https://orcid.org/0000-0001-8693-1073"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210088164","display_name":"Changchun Institute of Optics, Fine Mechanics and Physics","ror":"https://ror.org/012rct222","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bin Li","raw_affiliation_strings":["Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029612576","display_name":"Wenming Wu","orcid":"https://orcid.org/0000-0003-4793-6132"},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210088164","display_name":"Changchun Institute of Optics, Fine Mechanics and Physics","ror":"https://ror.org/012rct222","country_code":"CN","type":"facility","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenming Wu","raw_affiliation_strings":["Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China"],"raw_orcid":"https://orcid.org/0000-0003-4793-6132","affiliations":[{"raw_affiliation_string":"Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210088164"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1850,"currency":"USD","value_usd":1850},"apc_paid":{"value":1850,"currency":"USD","value_usd":1850},"fwci":0.2946,"has_fulltext":true,"cited_by_count":7,"citation_normalized_percentile":{"value":0.47465047,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"8","issue":null,"first_page":"99598","last_page":"99604"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9954000115394592,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polymerase-chain-reaction","display_name":"Polymerase chain reaction","score":0.49434351921081543},{"id":"https://openalex.org/keywords/bacteria","display_name":"Bacteria","score":0.4758172631263733},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.4750385582447052},{"id":"https://openalex.org/keywords/pipeline-transport","display_name":"Pipeline transport","score":0.4105266332626343},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.41038841009140015},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.25437766313552856},{"id":"https://openalex.org/keywords/environmental-science","display_name":"Environmental science","score":0.22900167107582092},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.20747783780097961},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.10514488816261292},{"id":"https://openalex.org/keywords/environmental-engineering","display_name":"Environmental engineering","score":0.06348028779029846},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.06133013963699341}],"concepts":[{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.49434351921081543},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4758172631263733},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4750385582447052},{"id":"https://openalex.org/C175309249","wikidata":"https://www.wikidata.org/wiki/Q725864","display_name":"Pipeline transport","level":2,"score":0.4105266332626343},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41038841009140015},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.25437766313552856},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.22900167107582092},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.20747783780097961},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10514488816261292},{"id":"https://openalex.org/C87717796","wikidata":"https://www.wikidata.org/wiki/Q146326","display_name":"Environmental engineering","level":1,"score":0.06348028779029846},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06133013963699341},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1109/access.2020.2996201","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2020.2996201","pdf_url":"https://ieeexplore.ieee.org/ielx7/6287639/8948470/09097833.pdf","source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:6ba436aae1094fa794c3d6ba5403a176","is_oa":true,"landing_page_url":"https://doaj.org/article/6ba436aae1094fa794c3d6ba5403a176","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"IEEE Access, Vol 8, Pp 99598-99604 (2020)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1109/access.2020.2996201","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2020.2996201","pdf_url":"https://ieeexplore.ieee.org/ielx7/6287639/8948470/09097833.pdf","source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G5256704237","display_name":null,"funder_award_id":"61704169","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G712620219","display_name":null,"funder_award_id":"20180520112JH","funder_id":"https://openalex.org/F4320310121","funder_display_name":"Natural Science Foundation of Jilin Province"}],"funders":[{"id":"https://openalex.org/F4320310121","display_name":"Natural Science Foundation of Jilin Province","ror":null},{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321133","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35"},{"id":"https://openalex.org/F4320322174","display_name":"People's Government of Jilin Province","ror":"https://ror.org/02fzqav45"},{"id":"https://openalex.org/F4320335787","display_name":"Fundamental Research Funds for the Central Universities","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3027246467.pdf","grobid_xml":"https://content.openalex.org/works/W3027246467.grobid-xml"},"referenced_works_count":27,"referenced_works":["https://openalex.org/W176843724","https://openalex.org/W971392733","https://openalex.org/W1530476857","https://openalex.org/W1562528808","https://openalex.org/W1987547769","https://openalex.org/W2029377448","https://openalex.org/W2033894232","https://openalex.org/W2040080388","https://openalex.org/W2042235985","https://openalex.org/W2043628508","https://openalex.org/W2054252662","https://openalex.org/W2064868974","https://openalex.org/W2084064323","https://openalex.org/W2085559524","https://openalex.org/W2099652529","https://openalex.org/W2102598922","https://openalex.org/W2130646906","https://openalex.org/W2170895924","https://openalex.org/W2210444572","https://openalex.org/W2738853303","https://openalex.org/W2776580484","https://openalex.org/W2892128733","https://openalex.org/W2932336123","https://openalex.org/W2956622757","https://openalex.org/W2982302778","https://openalex.org/W3082689487","https://openalex.org/W6782110519"],"related_works":["https://openalex.org/W4391375266","https://openalex.org/W2899084033","https://openalex.org/W2748952813","https://openalex.org/W2390279801","https://openalex.org/W2050457088","https://openalex.org/W4391913857","https://openalex.org/W2358668433","https://openalex.org/W4396701345","https://openalex.org/W2376932109","https://openalex.org/W2001405890"],"abstract_inverted_index":{"Polymerase":[0],"chain":[1],"reaction":[2],"(PCR)":[3],"technology":[4,8],"is":[5,99],"an":[6,27],"essential":[7],"for":[9,47,177],"point-of-care":[10],"testing":[11],"(POCT)":[12],"application.":[13],"To":[14],"realize":[15],"the":[16,42,66,70,77,90,94,103,114,122,134,145,158],"simple,":[17],"portable":[18,175],"and":[19,45,59,87,105,111,128,136,167,174],"fast":[20],"detection":[21,46,106],"of":[22,79,83,102,107,120,124,138,157,163],"bacteria":[23],"has":[24],"always":[25],"been":[26],"important":[28],"technology.":[29],"In":[30],"this":[31,139],"paper,":[32],"we":[33,116],"developed":[34,150],"a":[35,51,55,60,172],"pipeline-based":[36,74,95,146],"oil-bath":[37,96,147],"PCR":[38,43,67,97,148],"method,":[39],"to":[40,132],"achieve":[41],"amplification":[44,104],"bacteria.":[48,112],"We":[49],"assembled":[50],"small-sized":[52],"system":[53],"with":[54,69],"uniform":[56],"temperature":[57],"environment":[58],"stable":[61,173],"heating":[62],"module.":[63],"By":[64],"comparing":[65],"performance":[68],"other":[71],"two":[72,118],"existing":[73],"methods":[75],"on":[76],"reactions":[78],"both":[80,108],"DNA":[81,109],"fragment":[82,110],"H7N9":[84],"avian":[85],"influenza":[86],"Escherichia":[88],"Coli,":[89],"results":[91,142],"show":[92,143],"that":[93,144],"method":[98,149],"fully":[100],"capable":[101],"At":[113],"meanwhile,":[115],"performed":[117],"sets":[119],"tests,":[121],"experiment":[123],"pipeline":[125],"diameter":[126],"change":[127],"bacterial":[129],"concentration":[130],"gradient,":[131],"verify":[133],"sensitivity":[135],"universality":[137],"method.":[140],"The":[141],"by":[151],"us":[152],"not":[153],"only":[154],"gets":[155],"rid":[156],"complicated":[159],"preparations,":[160],"offers":[161],"advantages":[162],"simple":[164],"operation,":[165],"low-cost":[166],"user-friendly,":[168],"but":[169],"also":[170],"provides":[171],"foundation":[176],"our":[178],"later":[179],"work.":[180]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1}],"updated_date":"2026-07-22T07:51:19.307946","created_date":"2025-10-10T00:00:00"}