{"id":"https://openalex.org/W2902593458","doi":"https://doi.org/10.1109/access.2018.2884189","title":"Evolutionary Computation-Based Memetic Algorithm Against Genetic Algorithm to Improve PCR-RFLP Assay Primers of SNP Genotyping","display_name":"Evolutionary Computation-Based Memetic Algorithm Against Genetic Algorithm to Improve PCR-RFLP Assay Primers of SNP Genotyping","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2902593458","doi":"https://doi.org/10.1109/access.2018.2884189","mag":"2902593458"},"language":"en","primary_location":{"id":"doi:10.1109/access.2018.2884189","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2018.2884189","pdf_url":null,"source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1109/access.2018.2884189","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014905668","display_name":"Yu\u2010Huei Cheng","orcid":"https://orcid.org/0000-0002-1468-6686"},"institutions":[{"id":"https://openalex.org/I126145234","display_name":"Chaoyang University of Technology","ror":"https://ror.org/04xwksx09","country_code":"TW","type":"education","lineage":["https://openalex.org/I126145234"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Yu-Huei Cheng","raw_affiliation_strings":["Department of Information and Communication Engineering, Chaoyang University of Technology, Taichung, Taiwan"],"raw_orcid":"https://orcid.org/0000-0002-1468-6686","affiliations":[{"raw_affiliation_string":"Department of Information and Communication Engineering, Chaoyang University of Technology, Taichung, Taiwan","institution_ids":["https://openalex.org/I126145234"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019780601","display_name":"Ching\u2010Ming Lai","orcid":"https://orcid.org/0000-0002-7224-3955"},"institutions":[{"id":"https://openalex.org/I118292597","display_name":"National Taipei University of Technology","ror":"https://ror.org/00cn92c09","country_code":"TW","type":"education","lineage":["https://openalex.org/I118292597"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Ching-Ming Lai","raw_affiliation_strings":["Department of Vehicle Engineering, National Taipei University of Technology, Taipei, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Vehicle Engineering, National Taipei University of Technology, Taipei, Taiwan","institution_ids":["https://openalex.org/I118292597"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042023563","display_name":"Jiashen Teh","orcid":"https://orcid.org/0000-0001-9741-6245"},"institutions":[{"id":"https://openalex.org/I139322472","display_name":"Universiti Sains Malaysia","ror":"https://ror.org/02rgb2k63","country_code":"MY","type":"education","lineage":["https://openalex.org/I139322472"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Jiashen Teh","raw_affiliation_strings":["School of Electrical and Electronic Engineering, Universiti Sains Malaysia, Nibong Tebal, Malaysia"],"raw_orcid":"https://orcid.org/0000-0001-9741-6245","affiliations":[{"raw_affiliation_string":"School of Electrical and Electronic Engineering, Universiti Sains Malaysia, Nibong Tebal, Malaysia","institution_ids":["https://openalex.org/I139322472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037626830","display_name":"Che-Nan Kuo","orcid":"https://orcid.org/0000-0001-7705-4208"},"institutions":[{"id":"https://openalex.org/I38320133","display_name":"CTBC Business School","ror":"https://ror.org/04dmnxh66","country_code":"TW","type":"education","lineage":["https://openalex.org/I38320133"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Che-Nan Kuo","raw_affiliation_strings":["Department of Business Administration, CTBC Financial Management College, Tainan, Taiwan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Business Administration, CTBC Financial Management College, Tainan, Taiwan","institution_ids":["https://openalex.org/I38320133"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1850,"currency":"USD","value_usd":1850},"apc_paid":{"value":1850,"currency":"USD","value_usd":1850},"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.09620201,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"6","issue":null,"first_page":"77807","last_page":"77815"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9972000122070312,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.995199978351593,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genotyping","display_name":"Genotyping","score":0.8527652025222778},{"id":"https://openalex.org/keywords/restriction-fragment-length-polymorphism","display_name":"Restriction fragment length polymorphism","score":0.7681236267089844},{"id":"https://openalex.org/keywords/snp-genotyping","display_name":"SNP genotyping","score":0.7317242622375488},{"id":"https://openalex.org/keywords/restriction-enzyme","display_name":"Restriction enzyme","score":0.6466811895370483},{"id":"https://openalex.org/keywords/molecular-inversion-probe","display_name":"Molecular Inversion Probe","score":0.61346834897995},{"id":"https://openalex.org/keywords/snp","display_name":"SNP","score":0.5811558961868286},{"id":"https://openalex.org/keywords/primer","display_name":"Primer (cosmetics)","score":0.4987633228302002},{"id":"https://openalex.org/keywords/single-nucleotide-polymorphism","display_name":"Single-nucleotide polymorphism","score":0.4683994948863983},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.447174072265625},{"id":"https://openalex.org/keywords/melting-curve-analysis","display_name":"Melting curve analysis","score":0.4364444613456726},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.4336668848991394},{"id":"https://openalex.org/keywords/polymerase-chain-reaction","display_name":"Polymerase chain reaction","score":0.42885956168174744},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.32805120944976807},{"id":"https://openalex.org/keywords/genotype","display_name":"Genotype","score":0.32487934827804565},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.15176990628242493},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.1028645932674408},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.08055120706558228}],"concepts":[{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.8527652025222778},{"id":"https://openalex.org/C168007829","wikidata":"https://www.wikidata.org/wiki/Q857299","display_name":"Restriction fragment length polymorphism","level":4,"score":0.7681236267089844},{"id":"https://openalex.org/C163691529","wikidata":"https://www.wikidata.org/wiki/Q7391886","display_name":"SNP genotyping","level":5,"score":0.7317242622375488},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.6466811895370483},{"id":"https://openalex.org/C59701697","wikidata":"https://www.wikidata.org/wiki/Q6895959","display_name":"Molecular Inversion Probe","level":5,"score":0.61346834897995},{"id":"https://openalex.org/C139275648","wikidata":"https://www.wikidata.org/wiki/Q17134011","display_name":"SNP","level":5,"score":0.5811558961868286},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.4987633228302002},{"id":"https://openalex.org/C153209595","wikidata":"https://www.wikidata.org/wiki/Q501128","display_name":"Single-nucleotide polymorphism","level":4,"score":0.4683994948863983},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.447174072265625},{"id":"https://openalex.org/C84133368","wikidata":"https://www.wikidata.org/wiki/Q6813768","display_name":"Melting curve analysis","level":4,"score":0.4364444613456726},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4336668848991394},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.42885956168174744},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.32805120944976807},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.32487934827804565},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.15176990628242493},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1028645932674408},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08055120706558228},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1109/access.2018.2884189","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2018.2884189","pdf_url":null,"source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:bd8c96736b4a44f9807bad85a542d5a5","is_oa":true,"landing_page_url":"https://doaj.org/article/bd8c96736b4a44f9807bad85a542d5a5","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"IEEE Access, Vol 6, Pp 77807-77815 (2018)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1109/access.2018.2884189","is_oa":true,"landing_page_url":"https://doi.org/10.1109/access.2018.2884189","pdf_url":null,"source":{"id":"https://openalex.org/S2485537415","display_name":"IEEE Access","issn_l":"2169-3536","issn":["2169-3536"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319808","host_organization_name":"Institute of Electrical and Electronics Engineers","host_organization_lineage":["https://openalex.org/P4310319808"],"host_organization_lineage_names":["Institute of Electrical and Electronics Engineers"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IEEE Access","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G1255042389","display_name":"Innovative High-Throughput Multi-Point Mutagenic Primer Design for Genotyping Single Nucleotide Polymorphisms","funder_award_id":"MOST107-2221-E324-020","funder_id":"https://openalex.org/F4320322795","funder_display_name":"Ministry of Science and Technology, Taiwan"},{"id":"https://openalex.org/G6845830881","display_name":"A Portable Cloud Long-Term System with Mobile Medical Applications - Development of Expandable Integrated Biomedical Signal Extraction Device","funder_award_id":"MOST107-2622-E324-002-CC3","funder_id":"https://openalex.org/F4320322795","funder_display_name":"Ministry of Science and Technology, Taiwan"},{"id":"https://openalex.org/G8457390545","display_name":null,"funder_award_id":"MOST107-2821-C-324-001-ES","funder_id":"https://openalex.org/F4320322795","funder_display_name":"Ministry of Science and Technology, Taiwan"}],"funders":[{"id":"https://openalex.org/F4320322795","display_name":"Ministry of Science and Technology, Taiwan","ror":"https://ror.org/02kv4zf79"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1583721197","https://openalex.org/W1778913906","https://openalex.org/W1809786947","https://openalex.org/W1965747182","https://openalex.org/W1991452997","https://openalex.org/W2012570686","https://openalex.org/W2020246934","https://openalex.org/W2022716620","https://openalex.org/W2022919035","https://openalex.org/W2029292949","https://openalex.org/W2036402320","https://openalex.org/W2045523100","https://openalex.org/W2050617172","https://openalex.org/W2053009004","https://openalex.org/W2053962611","https://openalex.org/W2059186085","https://openalex.org/W2059562367","https://openalex.org/W2062373810","https://openalex.org/W2072579680","https://openalex.org/W2090018380","https://openalex.org/W2091963315","https://openalex.org/W2107421761","https://openalex.org/W2122732537","https://openalex.org/W2123950145","https://openalex.org/W2133620956","https://openalex.org/W2135995702","https://openalex.org/W2138833955","https://openalex.org/W2140609744","https://openalex.org/W2149788957","https://openalex.org/W2156256232","https://openalex.org/W2296457500","https://openalex.org/W2322200642","https://openalex.org/W2505614110","https://openalex.org/W2805967232","https://openalex.org/W6638040569"],"related_works":["https://openalex.org/W2153663358","https://openalex.org/W2914786560","https://openalex.org/W2350226114","https://openalex.org/W1551009089","https://openalex.org/W1612043471","https://openalex.org/W2416752024","https://openalex.org/W2155886944","https://openalex.org/W2578378181","https://openalex.org/W2164805996","https://openalex.org/W306073491"],"abstract_inverted_index":{"A":[0,58,95],"genetic":[1],"algorithm":[2,60],"(GA)":[3],"combines":[4],"the":[5,70,87,109,112,119,129,154,165,172,187,195,214,218],"restriction":[6,15,79,93,176],"enzyme":[7],"mining":[8],"core":[9],"of":[10,49,111,131,167,171,181],"single":[11],"nucleotide":[12],"polymorphism":[13,18],"(SNP)":[14],"fragment":[16],"length":[17],"(RFLP)":[19],"to":[20,64,73,90,97,148,186,204],"design":[21,48,65,205],"polymerase":[22],"chain":[23],"reaction":[24],"(PCR)-RFLP":[25],"primer":[26,102,188],"pairs":[27],"for":[28,53,69,118,164,175,194,209,224],"SNP-based":[29],"genotyping":[30,55,151],"with":[31,128,153,213],"feasible":[32,223],"estimated":[33],"GA":[34,38,113,155,216],"parameters.":[35],"However,":[36],"this":[37],"method":[39,89,121,198,220],"is":[40,56,221],"easily":[41],"trapped":[42],"into":[43,86],"local":[44],"optima.":[45],"An":[46],"improved":[47],"PCR-RFLP":[50,71,146,207,225],"assay":[51,72],"primers":[52,68,147,182,208],"SNP":[54,75,150,210,226],"needed.":[57],"memetic":[59],"(MA)":[61],"was":[62,84,105],"used":[63],"more":[66,99,222],"robust":[67,145,206],"enable":[74],"genotyping.":[76,211,227],"The":[77,190],"novel":[78,173],"enzymes":[80,177],"hunting":[81],"(REHUNT)":[82],"package":[83],"embedded":[85],"MA":[88,120,138,197,219],"provide":[91],"available":[92],"enzymes.":[94],"formula":[96,163],"calculate":[98],"accurate":[100,160],"thermodynamic":[101,161],"melting":[103,168],"temperatures":[104],"also":[106],"introduced.":[107],"Using":[108],"criteria":[110],"method,":[114,217],"in":[115,143],"silico":[116],"simulations":[117],"under":[122],"different":[123],"parameter":[124,139,192],"settings":[125,140,193],"were":[126,135,141,199],"performed":[127],"SNPs":[130],"SLC6A4,":[132],"and":[133,179,201],"results":[134],"compared.":[136],"Appropriate":[137],"superior":[142],"providing":[144],"achieve":[149],"compared":[152],"method.":[156],"Improvements":[157],"included":[158],"an":[159],"SantaLucia's":[162],"calculation":[166],"temperature,":[169],"use":[170],"REHUNT":[174],"mining,":[178],"selection":[180],"that":[183],"better":[184],"conformed":[185],"constraints.":[189],"appropriate":[191],"proposed":[196],"identified":[200],"carefully":[202],"evaluated":[203],"Compared":[212],"former":[215]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2026-08-01T09:00:35.917206","created_date":"2025-10-10T00:00:00"}