{"id":"https://openalex.org/W2099524123","doi":"https://doi.org/10.1093/bioinformatics/bts005","title":"Predicting folding free energy changes upon single point mutations","display_name":"Predicting folding free energy changes upon single point mutations","publication_year":2012,"publication_date":"2012-01-11","ids":{"openalex":"https://openalex.org/W2099524123","doi":"https://doi.org/10.1093/bioinformatics/bts005","mag":"2099524123"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/bts005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts005","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/28/5/664/16910996/bts005.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/bioinformatics/article-pdf/28/5/664/16910996/bts005.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100443049","display_name":"Zhe Zhang","orcid":"https://orcid.org/0000-0003-3983-1226"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Zhe Zhang","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115695074","display_name":"Lin Wang","orcid":"https://orcid.org/0000-0002-1588-588X"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lin Wang","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013879601","display_name":"Yang Gao","orcid":"https://orcid.org/0000-0001-5235-0713"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yang Gao","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100436742","display_name":"Jie Zhang","orcid":"https://orcid.org/0000-0002-4322-9118"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jie Zhang","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008380283","display_name":"Maksym Zhenirovskyy","orcid":"https://orcid.org/0000-0002-5478-0669"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maxim Zhenirovskyy","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5075727451","display_name":"Emil Alexov","orcid":"https://orcid.org/0000-0001-5346-0156"},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emil Alexov","raw_affiliation_strings":["1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA"],"affiliations":[{"raw_affiliation_string":"1 Computational Biophysics and Bioinformatics, Department of Physics and 2Department of Computer Science, Clemson University, Clemson, SC 29634, USA","institution_ids":["https://openalex.org/I8078737"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5100443049"],"corresponding_institution_ids":["https://openalex.org/I8078737"],"apc_list":{"value":3618,"currency":"USD","value_usd":3618},"apc_paid":null,"fwci":4.0216,"has_fulltext":false,"cited_by_count":99,"citation_normalized_percentile":{"value":0.94737772,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":"28","issue":"5","first_page":"664","last_page":"671"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9977999925613403,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/folding","display_name":"Folding (DSP implementation)","score":0.6516358852386475},{"id":"https://openalex.org/keywords/protein-data-bank","display_name":"Protein Data Bank (RCSB PDB)","score":0.6243008971214294},{"id":"https://openalex.org/keywords/point-mutation","display_name":"Point mutation","score":0.6188764572143555},{"id":"https://openalex.org/keywords/protein-folding","display_name":"Protein folding","score":0.5744982957839966},{"id":"https://openalex.org/keywords/mutation","display_name":"Mutation","score":0.5619717836380005},{"id":"https://openalex.org/keywords/stability","display_name":"Stability (learning theory)","score":0.5214656591415405},{"id":"https://openalex.org/keywords/in-silico","display_name":"In silico","score":0.5043123960494995},{"id":"https://openalex.org/keywords/missense-mutation","display_name":"Missense mutation","score":0.4353828728199005},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.4146520793437958},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.3701351284980774},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.3326755166053772},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.31638455390930176},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.31086987257003784},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.16183975338935852},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.1473836898803711},{"id":"https://openalex.org/keywords/machine-learning","display_name":"Machine learning","score":0.08103218674659729}],"concepts":[{"id":"https://openalex.org/C2776545253","wikidata":"https://www.wikidata.org/wiki/Q5464292","display_name":"Folding (DSP implementation)","level":2,"score":0.6516358852386475},{"id":"https://openalex.org/C65556437","wikidata":"https://www.wikidata.org/wiki/Q766195","display_name":"Protein Data Bank (RCSB PDB)","level":2,"score":0.6243008971214294},{"id":"https://openalex.org/C176944494","wikidata":"https://www.wikidata.org/wiki/Q1415380","display_name":"Point mutation","level":4,"score":0.6188764572143555},{"id":"https://openalex.org/C204328495","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein folding","level":2,"score":0.5744982957839966},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.5619717836380005},{"id":"https://openalex.org/C112972136","wikidata":"https://www.wikidata.org/wiki/Q7595718","display_name":"Stability (learning theory)","level":2,"score":0.5214656591415405},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.5043123960494995},{"id":"https://openalex.org/C75563809","wikidata":"https://www.wikidata.org/wiki/Q2656896","display_name":"Missense mutation","level":4,"score":0.4353828728199005},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4146520793437958},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3701351284980774},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3326755166053772},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.31638455390930176},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31086987257003784},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16183975338935852},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1473836898803711},{"id":"https://openalex.org/C119857082","wikidata":"https://www.wikidata.org/wiki/Q2539","display_name":"Machine learning","level":1,"score":0.08103218674659729},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C119599485","wikidata":"https://www.wikidata.org/wiki/Q43035","display_name":"Electrical engineering","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1093/bioinformatics/bts005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts005","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/28/5/664/16910996/bts005.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2581821","is_oa":false,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3289912","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/bts005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts005","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/28/5/664/16910996/bts005.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/7","display_name":"Affordable and clean energy","score":0.8999999761581421}],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2099524123.pdf","grobid_xml":"https://content.openalex.org/works/W2099524123.grobid-xml"},"referenced_works_count":97,"referenced_works":["https://openalex.org/W153460014","https://openalex.org/W1540550673","https://openalex.org/W1849447163","https://openalex.org/W1963697504","https://openalex.org/W1966743338","https://openalex.org/W1966979475","https://openalex.org/W1975580333","https://openalex.org/W1985006546","https://openalex.org/W1988915532","https://openalex.org/W1996201041","https://openalex.org/W2007886752","https://openalex.org/W2008628885","https://openalex.org/W2012490272","https://openalex.org/W2015357682","https://openalex.org/W2015872239","https://openalex.org/W2019574765","https://openalex.org/W2020248033","https://openalex.org/W2023490488","https://openalex.org/W2026339550","https://openalex.org/W2028843689","https://openalex.org/W2029903626","https://openalex.org/W2029999655","https://openalex.org/W2033140347","https://openalex.org/W2041189078","https://openalex.org/W2047730466","https://openalex.org/W2052868118","https://openalex.org/W2054243594","https://openalex.org/W2055607709","https://openalex.org/W2057646632","https://openalex.org/W2062436870","https://openalex.org/W2063253068","https://openalex.org/W2063274819","https://openalex.org/W2069297570","https://openalex.org/W2070179450","https://openalex.org/W2070989224","https://openalex.org/W2074727774","https://openalex.org/W2074756868","https://openalex.org/W2075481658","https://openalex.org/W2079306416","https://openalex.org/W2083624153","https://openalex.org/W2084936733","https://openalex.org/W2085726941","https://openalex.org/W2085764610","https://openalex.org/W2090004564","https://openalex.org/W2090847673","https://openalex.org/W2091076174","https://openalex.org/W2092387745","https://openalex.org/W2093847279","https://openalex.org/W2096635602","https://openalex.org/W2102697248","https://openalex.org/W2103449051","https://openalex.org/W2103866301","https://openalex.org/W2107191159","https://openalex.org/W2109309047","https://openalex.org/W2109372707","https://openalex.org/W2110068166","https://openalex.org/W2113710405","https://openalex.org/W2116075649","https://openalex.org/W2116502736","https://openalex.org/W2118233996","https://openalex.org/W2123340910","https://openalex.org/W2124406670","https://openalex.org/W2125677968","https://openalex.org/W2129651861","https://openalex.org/W2130479394","https://openalex.org/W2132262459","https://openalex.org/W2135503340","https://openalex.org/W2135815512","https://openalex.org/W2136206140","https://openalex.org/W2136513422","https://openalex.org/W2141109380","https://openalex.org/W2144482528","https://openalex.org/W2144840308","https://openalex.org/W2145644903","https://openalex.org/W2146043630","https://openalex.org/W2148830266","https://openalex.org/W2152279845","https://openalex.org/W2152486318","https://openalex.org/W2152949473","https://openalex.org/W2160185798","https://openalex.org/W2164561430","https://openalex.org/W2165304304","https://openalex.org/W2166744107","https://openalex.org/W2166765429","https://openalex.org/W2170342322","https://openalex.org/W2171741848","https://openalex.org/W2181723522","https://openalex.org/W2407287612","https://openalex.org/W2414035669","https://openalex.org/W2473551532","https://openalex.org/W2916078022","https://openalex.org/W4244205805","https://openalex.org/W4285719527","https://openalex.org/W6632273482","https://openalex.org/W6664947879","https://openalex.org/W6676993510","https://openalex.org/W6681811301"],"related_works":["https://openalex.org/W2997189992","https://openalex.org/W2260493136","https://openalex.org/W2113188411","https://openalex.org/W4210423528","https://openalex.org/W2976965029","https://openalex.org/W2023600921","https://openalex.org/W2382178265","https://openalex.org/W2055827177","https://openalex.org/W2072666835","https://openalex.org/W4248706500"],"abstract_inverted_index":{"Abstract":[0],"Motivation:":[1],"The":[2,21,147,200],"folding":[3,72,171,210],"free":[4,73,106,172,211],"energy":[5,74,107,212],"is":[6,14,64,123,202],"an":[7,101],"important":[8],"characteristic":[9],"of":[10,23,50,57,70,85,89,118,127,144,162,169,186,208],"proteins":[11],"stability":[12,25,56],"and":[13,140,154,184,195,217],"directly":[15],"related":[16],"to":[17,27,46,54,66,81,93,222],"protein's":[18,24],"wild-type":[19,120],"function.":[20],"changes":[22,168,213],"due":[26],"naturally":[28],"occurring":[29],"mutations,":[30,32],"missense":[31],"are":[33,43,150,157,246],"typically":[34],"causing":[35],"diseases.":[36],"Single":[37],"point":[38,77,111],"mutations":[39,78,149,164,216],"made":[40],"in":[41,79,152,176,232],"vitro":[42],"frequently":[44],"used":[45,231],"assess":[47],"the":[48,55,58,68,71,86,90,105,115,119,128,155,170,187,192,196,227],"contribution":[49],"given":[51],"amino":[52],"acid":[53],"protein.":[59,121],"In":[60],"both":[61],"cases,":[62],"it":[63],"desirable":[65],"predict":[67],"change":[69,91,108],"upon":[75,109,214],"single":[76,110,215],"order":[80],"either":[82],"provide":[83],"insights":[84],"molecular":[87,129],"mechanism":[88],"or":[92],"design":[94],"new":[95],"experimental":[96,193],"studies.":[97],"Results:":[98],"We":[99],"report":[100],"approach":[102],"that":[103],"predicts":[104],"mutation":[112],"by":[113],"utilizing":[114,141],"3D":[116],"structure":[117],"It":[122],"based":[124],"on":[125],"variation":[126],"mechanics":[130],"Generalized":[131],"Born":[132],"(MMGB)":[133],"method,":[134],"scaled":[135],"with":[136,165,204,220],"optimized":[137],"parameters":[138],"(sMMGB)":[139],"specific":[142],"model":[143],"unfolded":[145],"state.":[146],"corresponding":[148],"built":[151],"silico":[153],"predictions":[156],"tested":[158],"against":[159],"large":[160],"dataset":[161],"1109":[163],"experimentally":[166],"measured":[167],"energy.":[173],"Benchmarking":[174],"resulted":[175],"root":[177],"mean":[178],"square":[179],"deviation":[180],"=":[181],"1.78":[182],"kcal/mol":[183],"slope":[185],"linear":[188],"regression":[189],"fit":[190],"between":[191],"data":[194,245],"calculations":[197],"was":[198],"1.04.":[199],"sMMGB":[201],"compared":[203],"other":[205],"leading":[206],"methods":[207],"predicting":[209],"results":[218],"discussed":[219],"respect":[221],"various":[223],"parameters.":[224],"Availability:":[225],"All":[226],"pdb":[228],"files":[229],"we":[230],"this":[233],"article":[234],"can":[235],"be":[236],"downloaded":[237],"from":[238],"http://compbio.clemson.edu/downloadDir/mentaldisorders/sMMGB_pdb.rar":[239],"Contact:":[240],"ealexov@clemson.edu":[241],"Supplementary":[242,244],"information:":[243],"available":[247],"at":[248],"Bioinformatics":[249],"online.":[250]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":9},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":11},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":7},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":6},{"year":2013,"cited_by_count":9},{"year":2012,"cited_by_count":5}],"updated_date":"2026-03-10T16:38:18.471706","created_date":"2016-06-24T00:00:00"}
