{"id":"https://openalex.org/W2126784042","doi":"https://doi.org/10.1093/bioinformatics/btr065","title":"uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application","display_name":"uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application","publication_year":2011,"publication_date":"2011-02-07","ids":{"openalex":"https://openalex.org/W2126784042","doi":"https://doi.org/10.1093/bioinformatics/btr065","mag":"2126784042"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/btr065","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btr065","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/27/7/1019/48867347/bioinformatics_27_7_1019.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/bioinformatics/article-pdf/27/7/1019/48867347/bioinformatics_27_7_1019.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070177905","display_name":"Zachary Dwight","orcid":null},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Zachary Dwight","raw_affiliation_strings":["1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"],"affiliations":[{"raw_affiliation_string":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA","institution_ids":["https://openalex.org/I223532165"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018572080","display_name":"Robert Palais","orcid":null},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert Palais","raw_affiliation_strings":["1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"],"affiliations":[{"raw_affiliation_string":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA","institution_ids":["https://openalex.org/I223532165"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051507868","display_name":"Carl T. Wittwer","orcid":"https://orcid.org/0000-0002-9993-8263"},"institutions":[{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carl T. Wittwer","raw_affiliation_strings":["1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"],"affiliations":[{"raw_affiliation_string":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA","institution_ids":["https://openalex.org/I223532165"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5070177905"],"corresponding_institution_ids":["https://openalex.org/I223532165"],"apc_list":{"value":3618,"currency":"USD","value_usd":3618},"apc_paid":null,"fwci":4.5355,"has_fulltext":false,"cited_by_count":253,"citation_normalized_percentile":{"value":0.95700888,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"27","issue":"7","first_page":"1019","last_page":"1020"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9987000226974487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9987000226974487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.996999979019165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9968000054359436,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/melting-temperature","display_name":"Melting temperature","score":0.44795456528663635},{"id":"https://openalex.org/keywords/amplicon","display_name":"Amplicon","score":0.44393259286880493},{"id":"https://openalex.org/keywords/melting-point","display_name":"Melting point","score":0.4186604619026184},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.4152003228664398},{"id":"https://openalex.org/keywords/materials-science","display_name":"Materials science","score":0.3259972929954529}],"concepts":[{"id":"https://openalex.org/C2985276288","wikidata":"https://www.wikidata.org/wiki/Q16873139","display_name":"Melting temperature","level":2,"score":0.44795456528663635},{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.44393259286880493},{"id":"https://openalex.org/C13530604","wikidata":"https://www.wikidata.org/wiki/Q15318","display_name":"Melting point","level":2,"score":0.4186604619026184},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4152003228664398},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3259972929954529},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1093/bioinformatics/btr065","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btr065","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/27/7/1019/48867347/bioinformatics_27_7_1019.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.981.1725","is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.981.1725","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://bioinformatics.oxfordjournals.org/content/early/2011/02/07/bioinformatics.btr065.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/btr065","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btr065","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/27/7/1019/48867347/bioinformatics_27_7_1019.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2126784042.pdf","grobid_xml":"https://content.openalex.org/works/W2126784042.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1964568874","https://openalex.org/W1968860042","https://openalex.org/W1988361470","https://openalex.org/W1989162094","https://openalex.org/W1996259602","https://openalex.org/W2004541345","https://openalex.org/W2009095852","https://openalex.org/W2010759469","https://openalex.org/W2051753857","https://openalex.org/W2054115419","https://openalex.org/W2070484449","https://openalex.org/W2088306370","https://openalex.org/W2088998577","https://openalex.org/W2097632362","https://openalex.org/W2119699021","https://openalex.org/W2133935621","https://openalex.org/W2135995702","https://openalex.org/W2138753036","https://openalex.org/W2143559628","https://openalex.org/W2144145255","https://openalex.org/W2147851569","https://openalex.org/W2150705960"],"related_works":["https://openalex.org/W2097640472","https://openalex.org/W2055376970","https://openalex.org/W2082856704","https://openalex.org/W1655211217","https://openalex.org/W2804790611","https://openalex.org/W1893846133","https://openalex.org/W2032693502","https://openalex.org/W2100878359","https://openalex.org/W3003938081","https://openalex.org/W2000498896"],"abstract_inverted_index":{"Abstract":[0],"Summary:":[1],"uMeltSM":[2],"is":[3,221],"a":[4,27,49,92,105,192],"flexible":[5],"web-based":[6],"tool":[7],"for":[8,176,195],"predicting":[9],"DNA":[10],"melting":[11,146,153,162,179,188,201],"curves":[12,86],"and":[13,25,31,45,48,66,71,155,170,181,197,204,212],"denaturation":[14],"profiles":[15,101],"of":[16,29,94,107,125,151,199],"PCR":[17],"products.":[18],"The":[19,123,206],"user":[20],"defines":[21],"an":[22,53],"amplicon":[23],"sequence":[24,77,108],"chooses":[26],"set":[28],"thermodynamic":[30,168],"experimental":[32],"parameters":[33,169],"that":[34],"include":[35],"nearest":[36],"neighbor":[37],"stacking":[38],"energies,":[39],"loop":[40],"entropy":[41],"effects,":[42],"cation":[43],"(monovalent":[44],"Mg++)":[46],"concentrations":[47],"temperature":[50,83,95,130],"range.":[51,84],"Using":[52],"accelerated":[54],"partition":[55],"function":[56,93,106],"algorithm":[57],"along":[58],"with":[59,128,165],"chosen":[60],"parameter":[61],"values,":[62],"uMelt":[63,190],"interactively":[64],"calculates":[65],"visualizes":[67],"the":[68,72,82,88,117,140,149,160,166,177],"mean":[69,89],"helicity":[70,90,113,118,126],"dissociation":[73],"probability":[74,119],"at":[75,79,120,217,236],"each":[76],"position":[78,109],"temperatures":[80,114,163],"within":[81,139],"Predicted":[85,100],"display":[87,102],"as":[91,97,104,111,116],"or":[96,115],"derivative":[98],"plots.":[99],"stability":[103],"either":[110],"50%":[112],"specific":[121],"temperatures.":[122,158],"loss":[124],"associated":[127],"increasing":[129],"may":[131],"be":[132,214],"viewed":[133],"dynamically":[134],"to":[135,223],"visualize":[136],"domain":[137],"formation":[138],"molecule.":[141],"Results":[142],"from":[143],"fluorescent":[144,187],"high-resolution":[145,200],"experiments":[147],"match":[148],"number":[150],"predicted":[152],"domains":[154],"their":[156],"relative":[157],"However,":[159],"absolute":[161],"vary":[164],"selected":[167],"current":[171],"libraries":[172],"do":[173],"not":[174],"account":[175],"rapid":[178],"rates":[180],"helix":[182],"stabilizing":[183],"dyes":[184],"used":[185],"in":[186,210,225],"experiments.":[189],"provides":[191],"convenient":[193],"platform":[194],"simulation":[196],"design":[198],"assays.":[202],"Availability":[203],"implementation:":[205],"application":[207],"was":[208],"developed":[209],"Actionscript":[211],"can":[213],"found":[215],"online":[216],"http://www.dna.utah.edu/umelt/umelt.html.":[218],"Adobe":[219],"Flash":[220],"required":[222],"run":[224],"all":[226],"browsers.":[227],"Contact:":[228],"zach.dwight@path.utah.edu":[229],"Supplementary":[230,232],"information:":[231],"data":[233],"are":[234],"available":[235],"Bioinformatics":[237],"online.":[238]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":17},{"year":2024,"cited_by_count":24},{"year":2023,"cited_by_count":26},{"year":2022,"cited_by_count":16},{"year":2021,"cited_by_count":21},{"year":2020,"cited_by_count":23},{"year":2019,"cited_by_count":20},{"year":2018,"cited_by_count":19},{"year":2017,"cited_by_count":19},{"year":2016,"cited_by_count":18},{"year":2015,"cited_by_count":13},{"year":2014,"cited_by_count":10},{"year":2013,"cited_by_count":12},{"year":2012,"cited_by_count":11}],"updated_date":"2026-04-05T17:49:38.594831","created_date":"2025-10-10T00:00:00"}