{"id":"https://openalex.org/W2157205823","doi":"https://doi.org/10.1093/nar/gku410","title":"CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing","display_name":"CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing","publication_year":2014,"publication_date":"2014-05-26","ids":{"openalex":"https://openalex.org/W2157205823","doi":"https://doi.org/10.1093/nar/gku410","mag":"2157205823","pmid":"https://pubmed.ncbi.nlm.nih.gov/24861617"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gku410","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gku410","pdf_url":"https://academic.oup.com/nar/article-pdf/42/W1/W401/17423550/gku410.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/42/W1/W401/17423550/gku410.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011347709","display_name":"Tessa G. Montague","orcid":"https://orcid.org/0000-0002-5918-6327"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tessa G. Montague","raw_affiliation_strings":["Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018067069","display_name":"Jos\u00e9 Manuel Bautista Santa Cruz","orcid":null},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I2801851002","display_name":"Harvard University Press","ror":"https://ror.org/006v7bf86","country_code":"US","type":"other","lineage":["https://openalex.org/I136199984","https://openalex.org/I2801851002"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jos\u00e9 M. Cruz","raw_affiliation_strings":["Harvard School of Engineering and Applied Sciences, Cambridge, MA 02138, USA Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA","Harvard School of Engineering and Applied Sciences, Cambridge, MA 02138, USA","Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harvard School of Engineering and Applied Sciences, Cambridge, MA 02138, USA Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I2801851002"]},{"raw_affiliation_string":"Harvard School of Engineering and Applied Sciences, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025022209","display_name":"James A. Gagnon","orcid":"https://orcid.org/0000-0003-3978-6058"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James A. Gagnon","raw_affiliation_strings":["Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103076360","display_name":"George M. Church","orcid":"https://orcid.org/0000-0003-3535-2076"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George M. Church","raw_affiliation_strings":["Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA Department of Genetics, Harvard Medical School, Boston, MA 02115, USA","Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA","Department of Genetics, Harvard Medical School, Boston, MA 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA Department of Genetics, Harvard Medical School, Boston, MA 02115, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Genetics, Harvard Medical School, Boston, MA 02115, USA","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067360861","display_name":"Eivind Valen","orcid":"https://orcid.org/0000-0003-1840-6108"},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Eivind Valen","raw_affiliation_strings":["Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA eivind.valen@gmail.com","Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA eivind.valen@gmail.com","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA","institution_ids":["https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5067360861"],"corresponding_institution_ids":["https://openalex.org/I136199984"],"apc_list":{"value":3630,"currency":"USD","value_usd":3630},"apc_paid":{"value":3630,"currency":"USD","value_usd":3630},"fwci":29.3765,"has_fulltext":true,"cited_by_count":1330,"citation_normalized_percentile":{"value":0.99845794,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":100},"biblio":{"volume":"42","issue":"W1","first_page":"W401","last_page":"W407"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9975000023841858,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":0.9901999831199646,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transcription-activator-like-effector-nuclease","display_name":"Transcription activator-like effector nuclease","score":0.8205907344818115},{"id":"https://openalex.org/keywords/genome-editing","display_name":"Genome editing","score":0.8021253943443298},{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.8013293743133545},{"id":"https://openalex.org/keywords/cas9","display_name":"Cas9","score":0.7438093423843384},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.6949894428253174},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.60191810131073},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.549592912197113},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.4791014492511749},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.39397096633911133},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.3758503198623657}],"concepts":[{"id":"https://openalex.org/C31909778","wikidata":"https://www.wikidata.org/wiki/Q7833921","display_name":"Transcription activator-like effector nuclease","level":5,"score":0.8205907344818115},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8021253943443298},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8013293743133545},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7438093423843384},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6949894428253174},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.60191810131073},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.549592912197113},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4791014492511749},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.39397096633911133},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3758503198623657}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gku410","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gku410","pdf_url":"https://academic.oup.com/nar/article-pdf/42/W1/W401/17423550/gku410.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmid:24861617","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24861617","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":null},{"id":"pmh:oai:dash.harvard.edu:1/13581017","is_oa":true,"landing_page_url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:13581017","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:3221340","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4086086","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/nar/gku410","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gku410","pdf_url":"https://academic.oup.com/nar/article-pdf/42/W1/W401/17423550/gku410.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4399999976158142,"display_name":"Quality Education","id":"https://metadata.un.org/sdg/4"}],"awards":[{"id":"https://openalex.org/G2738422757","display_name":null,"funder_award_id":"P50 HG005550","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"}],"funders":[{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2157205823.pdf","grobid_xml":"https://content.openalex.org/works/W2157205823.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1493535861","https://openalex.org/W1930053815","https://openalex.org/W1976391205","https://openalex.org/W1977391294","https://openalex.org/W1978129709","https://openalex.org/W1982846895","https://openalex.org/W2000046950","https://openalex.org/W2003171404","https://openalex.org/W2006373538","https://openalex.org/W2025074364","https://openalex.org/W2026520402","https://openalex.org/W2036176224","https://openalex.org/W2045435533","https://openalex.org/W2064815984","https://openalex.org/W2073873559","https://openalex.org/W2077659966","https://openalex.org/W2089589904","https://openalex.org/W2101932407","https://openalex.org/W2108871359","https://openalex.org/W2112444085","https://openalex.org/W2114838239","https://openalex.org/W2115189270","https://openalex.org/W2115420193","https://openalex.org/W2120932131","https://openalex.org/W2123500370","https://openalex.org/W2124985265","https://openalex.org/W2132401721","https://openalex.org/W2133702185","https://openalex.org/W2135415614","https://openalex.org/W2138993899","https://openalex.org/W2143208261","https://openalex.org/W2149402050","https://openalex.org/W2154395280","https://openalex.org/W2156114027","https://openalex.org/W2158023793","https://openalex.org/W2162098634","https://openalex.org/W2164695230","https://openalex.org/W2166934949","https://openalex.org/W2167380395","https://openalex.org/W2170039745","https://openalex.org/W6669714862","https://openalex.org/W6676876283","https://openalex.org/W6677208431","https://openalex.org/W6680192589","https://openalex.org/W6683280080","https://openalex.org/W6683927505","https://openalex.org/W6684584065"],"related_works":["https://openalex.org/W2547051899","https://openalex.org/W3199043689","https://openalex.org/W2603161682","https://openalex.org/W2960163646","https://openalex.org/W2807464733","https://openalex.org/W2516842346","https://openalex.org/W2667943050","https://openalex.org/W2134268326","https://openalex.org/W2903635918","https://openalex.org/W2437118042"],"abstract_inverted_index":{"Major":[0],"advances":[1],"in":[2,40],"genome":[3,172],"editing":[4],"have":[5,56],"recently":[6],"been":[7],"made":[8],"possible":[9,141],"with":[10,122],"the":[11,14,48,65,120],"development":[12],"of":[13,23,32,52,73,86,107,119,155,164],"TALEN":[15,43],"and":[16,21,34,44,50,82,102,111,131,146,158,162],"CRISPR/Cas9":[17,45],"methods.":[18],"The":[19,160],"speed":[20,163],"ease":[22],"implementing":[24],"these":[25],"technologies":[26],"has":[27],"led":[28],"to":[29,63,98,134],"an":[30,58,84,116],"explosion":[31],"mutant":[33,156],"transgenic":[35],"organisms.":[36],"A":[37],"rate-limiting":[38],"step":[39],"efficiently":[41],"applying":[42],"methods":[46],"is":[47],"selection":[49],"design":[51,66],"targeting":[53],"constructs.":[54],"We":[55],"developed":[57],"online":[59],"tool,":[60],"CHOPCHOP":[61,68,165],"(https://chopchop.rc.fas.harvard.edu),":[62],"expedite":[64],"process.":[67],"accepts":[69],"a":[70,152,168],"wide":[71],"range":[72],"inputs":[74],"(gene":[75],"identifiers,":[76],"genomic":[77,129],"regions":[78],"or":[79],"pasted":[80],"sequences)":[81],"provides":[83],"array":[85],"advanced":[87],"options":[88],"for":[89,139,171],"target":[90,124,142],"selection.":[91],"It":[92],"uses":[93],"efficient":[94],"sequence":[95],"alignment":[96],"algorithms":[97],"minimize":[99],"search":[100],"times,":[101],"rigorously":[103],"predicts":[104],"off-target":[105],"binding":[106],"single-guide":[108],"RNAs":[109],"(sgRNAs)":[110],"TALENs.":[112],"Each":[113],"query":[114],"produces":[115],"interactive":[117],"visualization":[118],"gene":[121],"candidate":[123],"sites":[125,145],"displayed":[126],"at":[127],"their":[128],"positions":[130],"color-coded":[132],"according":[133],"quality":[135],"scores.":[136],"In":[137],"addition,":[138],"each":[140],"site,":[143],"restriction":[144],"primer":[147],"candidates":[148],"are":[149],"visualized,":[150],"facilitating":[151],"streamlined":[153],"pipeline":[154],"generation":[157],"validation.":[159],"ease-of-use":[161],"make":[166],"it":[167],"valuable":[169],"tool":[170],"engineering.":[173]},"counts_by_year":[{"year":2026,"cited_by_count":47},{"year":2025,"cited_by_count":119},{"year":2024,"cited_by_count":133},{"year":2023,"cited_by_count":109},{"year":2022,"cited_by_count":124},{"year":2021,"cited_by_count":131},{"year":2020,"cited_by_count":145},{"year":2019,"cited_by_count":168},{"year":2018,"cited_by_count":127},{"year":2017,"cited_by_count":83},{"year":2016,"cited_by_count":75},{"year":2015,"cited_by_count":52},{"year":2014,"cited_by_count":16},{"year":2013,"cited_by_count":1}],"updated_date":"2026-07-27T08:26:11.824852","created_date":"2025-10-10T00:00:00"}